Browsing by Author "Prashant Bisen"

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Comparative Study of Trichoderma BHU-1 Mediated Drought Alleviation of Susceptible and Tolerant Rice Cultivars
(Journal of Pure and Applied Microbiology, 2025) Ram Nandan Yadav; Renu Yadav; Najam Waris Zaidi; Harikesh Bahadur Singh; Prashant Bisen; Md Mahtab Rashid; Sudheer Kumar Yadav
The study investigated Trichoderma-mediated morphological and biochemical responses in droughtsusceptible and tolerant rice cultivars, namely IR-64 and DRR-44, under drought-stressed and normal conditions. Various morphological and biochemical parameters were recorded 30, 60, and 90 days after transplanting. The shoot length was insignificant, while the root length was significant in droughtsusceptible DRR-44 compared to non-stressed plants. The number of roots was also significant in Trichoderma BHU-1 treated plants of both cultivars. Proline content was more substantial in drought susceptible cultivars than tolerant and similarly, lignin, TPC, PAL, and PO activities were higher in Trichoderma BHU-1 treated drought-stressed plants than in normal ones. The result revealed that Trichoderma BHU-1 treatment modulates an increase in root length, shoot length, and the total number of tillers and roots under drought conditions. It also maintained the level of phenolics in plants by upregulating the pathway thereby helping the plant to sustain drought. © The Author(s) 2025.
Genetic analysis and trait association for yield related traits in F2:3 biparental population of rice under slightly sodic condition
(Indian Society of Plant Breeders, 2019) Prashant Bisen; P.K. Singh; Bapsila Loitongbam; Namrata Rathi; Sanket R. Rathi; Sameer Upadhyay; B. Sinha
Genetic variability and correlation studies provide basic information concerning the genetic properties of the population based on which, breeding methods could be formulated for further improvement of the crop. The estimates of heritability, coefficients of variability, genetic advance and trait association were computed for eleven yield related traits in F2:3 segregating populations of biparental crosses under slightly sodic condition. Analysis of variance revealed significant differences for the traits studied except, effective tillers per plant, panicle length and test weight, indicating the existence of high genetic variability among the lines. Estimates of phenotypic coefficients of variation (PCV) were higher than genotypic coefficient of variation (GCV) for all the characters studied, indicating considerable effect of environment. Moderate heritability accompanied with high genetic advance was recorded for filled grains and unfilled grains per panicle which, indicates heritability is most likely due to additive gene effect and selection for the traits will be effective. Traits like: effective tillers per plant, panicle length, filled grains per panicle and total grains per panicle that showed, positive and significant association with grain yield per plant, could be useful for breeders in any breeding program for further improvement of the traits under sodic condition. © 2019 Indian Society of Plant Breeders.
Genetic analysis of F4:F5 marker assisted derived lines of rice against bacterial leaf blight (BLB) and their marker validation
(Society for Plant Research, 2017) Shoumik Shah; Deep Rashmi; Bapsila Loitongbam; Prashant Bisen; V.K. Singh; Sandhya; U.S. Singh; P.K. Singh
The 23 F4:F5 lines of rice derived from the cross HUR 105 and IRBB 55 against bacterial leaf blight (BLB) at phenotypic and genetic levels were evaluated during Kharif 2015. The validation for the BLB resistance genes Xa21 and xa13 by the static markers pTA-248 and xa13pro conferring that these two genes were present in all the 23 F4:F5 rice lines. The highest value of heritability was obtained for plant height (89%) followed by number of effective tillers per plant (77.48%), test weight (74.81%) and grain yield per plant (73.66%). Positive and significant association were recorded between grain yield per plant with plant height, panicle weight, number of effective tillers, filled grains per panicle and total grains per panicle. Path analysis revealed direct and positive effect on grain yield per plant through traits like plant height, panicle weight, number of tillers, filled grains per panicle and total grains per panicle. Highest genetic advance was observed for total grains per panicle (31.87) followed by filled grains per panicle (22.84) and plant height (8.11). High heritability and high genetic advance may be attributed to additive gene action and simple selection could be applied for genetic improvement. © 2017, SPR, All Rights Reserved.
Genetic variability and association studies for blast disease and yield traits in F2 population of Cross Hur 3022 × TETEP in rice (Oryza sativa L.)
(Plant Archives, 2019) Namrata; R.P. Singh; Ramlakhan Verma; Prashant Bisen; Manish Kumar; Arsode Pandurang; Madhu Choudhary
Analysis of variance revealed significant differences for the traits studied except, weight of panicle and length breadth ratio of grain, indicating the existence of high genetic variability among individuals of F2 population. Less difference was observed between phenotypic coefficients of variation (PCV) and genotypic coefficients of variation (GCV) for all the characters studied, indicating absence of considerable effect of the environment. Higher value for both GCV and PCV were observed for number of effective tillers per plant, weight of panicle, number of filled grains per panicle, grain yield per plant, disease severity per cent and area under disease progress curve. High heritability with high genetic advance observed for number of filled grains per panicle, number of unfilled grains per panicle, spikelet fertility percent and area under disease progress curve hence selection will be highly effective for these traits. Number of effective tillers per plant, number of filled grains per panicle, plant height and spikelet fertility % showed significant positive correlation with grain yield per plant, thus performing selection for these traits will be resulted into improvement of grain yield per plant. Leaf blast infection cause significant decrease in grain yield per plant because both blast disease related traits (disease severity % and area under disease progress curve) in this study showed significant negative correlation with the grain yield per plant. © 2019 Plant Archives. All rights reserved.
Identification of QTLs for zinc deficiency tolerance in a recombinant inbred population of rice (Oryza sativa L.)
(John Wiley and Sons Ltd, 2022) Bapsila Loitongbam; Pawan Kumar Singh; Rameswar Prasad Sah; Om Prakash Verma; Balwant Singh; Prashant Bisen; Sandhya Kulhari; Sanket R Rathi; Sameer Upadhyay; Nagendra Kumar Singh; Rabin Sahu; Rakesh Kumar Singh
BACKGROUND: Deficiency of Zn is a major soil constraint in rice plant growth and yield. Edaphic factors such as Zn deficiency in soil in relation to plant performance are still poorly understood. Here, we report promising quantitative trait loci (QTL) conferring tolerance to Zn deficiency, which were identified through biparental mapping. The experiment was conducted using the 236 F7 recombinant inbred line mapping population derived from the cross of Kinandang Patong (Zn deficiency sensitive) and A69-1 (Zn deficiency tolerant). RESULTS: A total of six QTLs (qLB-2B, qLB-4B, qPM-4B, qPM-6B, qRZC-4B, qSZC-4B) on chromosomes 2, 4 and 6 were identified for environment 1, whereas five QTLs (qLB-2 N, qLB-4 N, qPM-4 N, qRZC-4 N, qSZC-4 N) on chromosomes 2 and 4 were detected for environment 2. Among these, five major (51.30, 48.70, 28.60, 56.00, 52.00 > 10 R2) and one minor (5.40 < 10 R2) QTLs for environment 1 and four major (51.48, 50.20, 53.00, 48.00 > 10 R2) and one minor (4.44 < 10) QTLs for environment 2 for Zn deficiency tolerance with a logarithm of odd threshold value higher than 3 were identified. The QTLs (qLB-4B, qPM-4B, qRZC-4B, qSZC-4B, qLB-4 N, qPM-4 N, qRZC-4 N, qSZC-4 N) for leaf bronzing, plant mortality root zinc concentration and shoot zinc concentration identified on chromosome 4 were found to be the most promising and highly reproducible across the locations that explained phenotypic variation from 48.00% to 56.00% with the same marker interval RM6748–RM303. CONCLUSION: The new QTLs and its linked markers identified in the present study can be utilized for Zn deficiency tolerance in elite cultivars using marker-assisted backcrossing. © 2022 Society of Chemical Industry. © 2022 Society of Chemical Industry.
Inheritance of blast disease resistance in the cross hur 3022 x tetep of rice (Oryza sativa l.)
(Editorial board of Journal of Experimental Biology and Agricultural Sciences, 2019) Namrata; R.P. Singh; Ramlakhan Verma; Prashant Bisen; Prakash Singh; Basavaraj Teli
An experiment was carried out using six generations (P1, P2, F1, F2, B1 and B2) of cross HUR 3022 x Tetep in rice at BHU, Varanasi during year 2017-2018 to know the inheritance pattern of leaf blast disease under artificial inoculation with LB-TN-2 isolate of Magnaporthe oryzae in the field condition. The blast disease resistant cultivar ‘Tetep’ showed 9.32% disease severity, while high yielding, early maturing susceptible cultivar HUR 3022 showed 43.65% disease severity against M. oryzae. The area under the disease progress curve (AUDPC) of resistance cultivar was observed 127.95 which are significantly less than the susceptible cultivar 605.62. The F1 (HUR 3022 x Tetep) plants were observed to be resistant with an average disease severity and AUDPC are 17.54% and 224.7, respectively. The F2 population was observed to show three distinct phenotypic classes resistant, moderately resistant and susceptible with a ratio of 9:6:1, respectively. Two backcross Populations, B1 and B2 showed different response from each other during evaluation which results in the phenotypic ratio of 1R:2MR:1S in B1 and 1R:0S in B2, respectively. The results showed that blast disease resistance occurs in the cross is due to duplicate cumulative effects or polymeric gene effect of two dominant resistant genes i.e., Pi1 and Pi54. © 2019, Editorial board of Journal of Experimental Biology and Agricultural Sciences. All rights reserved.
Sustainable production of rice under sodicity stress condition
(Springer Singapore, 2020) Sameer Upadhyay; P.K. Singh; S.R. Rathi; Prashant Bisen; Bapsila Loitongbam
In today's world population, explosion is not an unknown phenomenon and so is climate change. Resources are limited, and hence, the act of sustainable production comes into play in agriculture. The word sustainable production in agriculture aims at producing food not compromising the food security of future generation. Rice is one of the major cereals which feed the entire South East Asia and its neighbouring countries contributing a major share in dietary consumption, and on the other hand, sodicity stress is engulfing areas which could otherwise contribute towards increased rice production. Therefore, to achieve the target to feed the ever-growing population, it is of paramount importance to utilise the sodicity condition such that it should contribute towards rice production in a sustainable way. Growing science of agriculture will play a prominent role for increasing production and will provide an opportunity to achieve the objective of extirpating hunger and ensuring food security. © Springer Nature Singapore Pte Ltd. 2020. All rights reserved.
Variability analysis and character association for sodicity tolerance in F2:3 biparental population of rice under sodic condition
(Plant Archives, 2020) Prashant Bisen; P.K. Singh; Bapsila Loitongbam; Namrata; Ashok Singmasetti; B. Sinha
The present investigation was under taken with the objectives to estimate genetic variability for sodicity related traits and its association with grain yield in F2:3 segregating population under slightly sodic condition.. The experiment was conducted at the Agricultural Research Farm of Banaras Hindu University, Varanasi (U. P.), India during kharif, 2018. Analysis of variance revealed significant differences for the traits studied indicating the existence of high genetic variability among the lines. Estimates of phenotypic coefficients of variation (PCV) were higher than genotypic coefficient of variation (GCV) for all the characters studied, indicating considerable effect of environment. Tolerant parent CSR 43 performed better than sensitive parent Pusa 44 for all the traits studied under sodic stress. CSR 43 and the F2:3 lines which showed tolerance response, recorded low shoot Na+ concentration and Na+: K+ ratio, high shoot K+ concentration, total chlorophyll content and all together respond to gain higher yield than susceptible lines. Heritability was highest among the traits studied for total chlorophyll content. Selection for shoot Na+ concentration, Na+: K+ ratio and shoot K+ concentration, total chlorophyll content will support the breeder in any breeding program for further improvement of the traits under sodic condition as these traits showed significant association with grain yield per plant in favorable negative and positive direction, respectively. The present investigation was under taken with the objectives to estimate genetic variability for sodicity related traits and its association with grain yield in F2:3 segregating population under slightly sodic condition.. The experiment was conducted at the Agricultural Research Farm of Banaras Hindu University, Varanasi (U. P.), India during kharif, 2018. Analysis of variance revealed significant differences for the traits studied indicating the existence of high genetic variability among the lines. Estimates of phenotypic coefficients of variation (PCV) were higher than genotypic coefficient of variation (GCV) for all the characters studied, indicating considerable effect of environment. Tolerant parent CSR 43 performed better than sensitive parent Pusa 44 for all the traits studied under sodic stress. CSR 43 and the F2:3 lines which showed tolerance response, recorded low shoot Na+ concentration and Na+: K+ ratio, high shoot K+ concentration, total chlorophyll content and all together respond to gain higher yield than susceptible lines. Heritability was highest among the traits studied for total chlorophyll content. Selection for shoot Na+ concentration, Na+: K+ ratio and shoot K+ concentration, total chlorophyll content will support the breeder in any breeding program for further improvement of the traits under sodic condition as these traits showed significant association with grain yield per plant in favorable negative and positive direction, respectively. © 2020 Plant Archives. All rights reserved.