Browsing by Author "Pratibha Chaturvedi"

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Chloroplast derived SSRs reveals genetic relationships in domesticated alliums and wild relatives
(Springer Science and Business Media B.V., 2022) Kuldip Jayaswall; Himanshu Sharma; Abhishek Bhandawat; R. Sagar; Deepanshu Jayaswal; Akash Kumar; Pratibha Chaturvedi; Vijay Mahajan; Sanjay Kumar; Major Singh
Alliums comprise of popular spices and used for various culinary purposes and nutraceuticals. Poor genetic characterization and scarce information regarding domestication, evolution of important alliums like onion, garlic and wild relatives are the major bottleneck to alliums improvement programs. In present study, chloroplast simple sequence repeat (cpSSR) markers were developed from Allium paradoxum plastome to examine the genetic relationships among onion, garlic and wild germplasms. A total of 15 cpSSR markers were used to establish genetic relationships in 18 individuals of alliums, producing 40 alleles ranging from 1 to 6 alleles per SSR locus and polymorphism potential of 100%.Average PIC and heterozygosities was found to be 0.392 and 0.330, respectively. The developed cpSSR significantly assisted the phylogenetic study and evolutionary relationships among various Allium species. In future, these cpSSR markers will be useful for varietal identification, purity testing and genetic improvement of alliums. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
Development of chloroplast derived SSR markers for genus Allium and their characterization in the allies for genetic improvement of Alliums
(Elsevier B.V., 2023) Kuldip Jayaswall; Himanshu Sharma; Deepanshu Jayaswal; R. Sagar; Abhishek Bhandawat; Akash Kumar; Isha Sharma; Pratibha Chaturvedi; Dalasanuru Chandregowda Manjunathagowda; Deepesh Kumar; Vijay Mahajan; Sanjay Kumar; Major Singh
Alliums are the most popular for their culinary usage and nutraceutical benefits. Their production is greatly affected by the multiple biotic and abiotic stresses. Poor characterizations of genetic resources are the major bottleneck in the genetic improvement of Alliums. Chloroplast-derived simple sequence repeat has recently gained much popularity due to their maternal inheritance and low recombination along with their hypervariable nature. In this study, 22 of the 28 chloroplast-derived markers from chloroplast genomes of A. cepa and A. sativum were reported as polymorphic and cross-transferable. Tetra nucleotide repeats were more prevalent (52 %) in these 22 cpSSR markers as compared to other repeat motifs. Further polymorphic information content of these 22cpSSR markers ranged from 0.007 to 0.427. Based on the genotyping of 22cpSSR markers, 25 Alliums were clustered into two groups (groups I, IIA, and IIB). This showed that cultivated Allium cepa and Allium sativum belong to group II rather than the majority of wild Alliums, which belong to group I. Hence This proved the widespread use of these AccpSSR and AscpSSR markers. Additionally, it was clear from 79 Alliums morphological and genotyping-based clustering that these 79 wild Alliums exhibit strong clustering consistency in genotyping and phenotyping-based dendrograms, hence confirming these cpSSR's discrimination potential. Allium tuberosum Rottler ex. Spreng is quite distinct from Allium fistulosum L, A. schoenoprasumas as well as Allium cepa var. aggregatum G.Don. Further Allium fistulosum L, A. schoenoprasum and Allium cepa var. aggregatum are closely related, according to genotyping-based dendrograms of 79 wild Alliums. Hence Allium fistulosum L, A. schoenoprasum, and Allium cepa provide a brilliant opportunity for introgression of the high total soluble content, high quercetin content, and allicin content of cultivated Allium into wild Allium as well as the biotic and abiotic stress tolerance of wild Alliums into cultivated Alliums. Subsequent clustering of 79 Alliums further allowed us to identify diverse Alliums for constructing a core collection. Hence present study will be useful for molecular breeding and genomic selection-based crop improvement. © 2023 SAAB
Ethnobotanical knowledge and socioeconomic potential of honey wine in the horn of Africa
(National Institute of Science Communication and Information Resources (NISCAIR), 2019) Anurag Dhyani; Kamal C. Semwal; Yishak Gebrekidan; Meheretu Yonas; Vinod Kumar Yadav; Pratibha Chaturvedi
The traditional honey wine is a ceremonial drink made locally in Ethiopia and Eritrea. The drink is known as Tej in Amharic (a widely spoken language in Ethiopia) and Mess in Tigrigna (a widely spoken language in Eritrea). It is consumed mostly during social and religious ceremonies, albeit sold in honey wine bars. It is easy to prepare with varied tastes by local people from its main components; honey, chopped stems of Rhamnus prinoides or roots of R. staddo and water. Honey and the shrubs used for the preparation of the wine are recognized for their medicinal importance worldwide. Particularly, after the isolation of geshoidin, a bitter glycoside from R. prinoides, that is currently being investigated for its role in providing novel-pharmacological leads for Alzheimer’s treatment. On the other hand, R. staddo has been investigated for potential antimalarial candidate. These with other beneficial metabolites from the shrubs call for a wider investigation into the medicinal benefits of the honey wine. Furthermore, considering its declining consumption, limited efforts to preserve the indigenous knowledge of preparing the drink and lack of adequate promotion, further research is needed to lead into the sustainability of drink for generations and its global usage. © 2019, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.
Exploring the Strategies, Utilisation and Limitations of Digital Tool Adoption in Sugarcane Farming
(Indian Society of Extension Education, 2025) Pratibha Chaturvedi; Lalita Vatta
The study investigates how sugarcane farmers are currently using digital technologies, identifies the obstacles to their efficient use, and suggests ways to improve the digitalization of sugarcane farming methods. The research was carried out during 2024 in Deoria (district) Sugarcane Committee’s Jaura Bazar centre “A”, Uttar Pradesh, which is the Deoria Sugarcane Committee’s highest-producing sugarcane centre. Purposive sampling was used to choose the sugarcane committee and centre, while random sampling method was used to select the villages and respondent farmers. Garrett’s ranking approach was used to assess the constraints of digital tools and technology as perceived by sugarcane cultivators. Data was collected from 230 respondents using a pre-structured interview schedule, revealing that mobile applications are mostly used for seed treatment and pest control in sugarcane farming, and they are less frequently used for other tasks. The primary barriers include technological challenges, budgetary limitations, and societal concerns. It is suggested to use strategies like offline apps, training, free subscriptions, and local language assistance to boost acceptability. © 2025, Indian Society of Extension Education. All rights reserved.
Validation of carbon contents in different traits and components of herbaceous species from tropical grassland
(Ecological Society of India, 2020) Pratibha Chaturvedi; Preeti Verma; R. Sagar; Hariom Verma; Kuldeep Kumar
Because of global climate change events; the quantification of carbon (C) present in different species from diverse ecosystems are urgently needed. Study evaluated the performances of different methods used for the C estimation in different plant components and traits by comparing with harvest method. For this, 117 herbaceous species just-before flowering were harvested from the campus of Banaras Hindu University, Varanasi. Across the components, the mean C contents (g plant-1) varied between 0.33 for roots of procumbent and 7.93 for shoots of native trait categories. On g m-2 basis, it ranged from 29 (roots) to 85 (shoots). Similarly across the components, the mean C: B ratio ranged between 0.41 (roots) and 0.44 (shoots). The linear relationship between C and B had high correlation coefficient, low standard error of estimate together with low discrepancy in extrapolation compared to the other models. Hence, compared to other models, the linear equation could be used in C estimation. Further among all the estimators; at the species, component and functional group levels, the mean C: B (0.43) ratio of the entire herbaceous species resulted as an accurate estimator of the C. Because of its simplicity and low discrepancy, the mean C: B (0.43) ratio seems to be reasonable to estimate the C content based on the known biomass. Stems of perennials, erects, leguminous and native plants had greater C than the others. Thus, the perennial, erect, leguminous and native herbaceous species could be an alternative for reducing the atmospheric C in the tropical grasslands. © 2020 Ecological Society of India. All rights reserved.
Variations of biomass and carbon contents in different traits and components of herbaceous species from tropical grassland
(African Science Publications, 2019) Preeti Verma; R. Sagar; Hariom Verma; Abhishek Rai; Pratibha Chaturvedi; Prem Pratap Singh; Kuldeep Kumar; Sandeep Kumar Singh
Grasslands play a critical role in the global storage of atmospheric carbon (C). Precise estimation of C contents in different plant components is essential to formulate a strategy for mitigating the atmospheric C. Biomass (B) and C of different herbaceous plant components at species, functional group and site levels from tropical grassland locating on the campus of Banaras Hindu University, Varanasi, India were estimated. For this; 117 herbaceous species just-before flowering were harvested. B and C contents for each species and component were measured and statistically analyzed. The measured C (g plant-1) across the components varied from 0.08 to 31.12. On gm–2 basis; it varied between 29 (leaf) and 49 (root). Plant components, species and functional groups in isolation caused significant differences in the measured C. In the present study; the C content of stem was greater than the leaf and root. The perennial, erect, leguminous and native traits had greater C than the others. Therefore, this observation revealed that the perennial, erect, leguminous and native plants could be a better option for reducing the atmospheric CO2 by capturing it and then converting into B through photosynthesis. Further, the fitted regression equation between the root and shoot for B and C could be used for the extrapolation of B and C of the root component based on the shoot component. The conservative field measurement methods may give precise data on B and C but are destructive to grassland, difficult, time-consuming, and costly to cover at large scale. Hence, the present work could be substantial for the estimation of root C based on shoot component. © 2019 African Journal of Biological Sciences. All rights reserved.