Browsing by Author "Praveen Gupta"

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24-Epibrassinolide and Sodium Nitroprusside alleviate the salinity stress in Brassica juncea L. cv. Varuna through cross talk among proline, nitrogen metabolism and abscisic acid
(Springer International Publishing, 2017) Praveen Gupta; Sudhakar Srivastava; Chandra Shekhar Seth
Background and aim: Soil salinity is one of the major environmental obstacles that limit the crop productivity. A pot experiment was conducted with an aim to explore the ameliorative effects of exogenously applied 24-epibrassinolide (EBL) and sodium nitroprusside (SNP) in alone and/or combination against salinity stress in Brassica juncea (L.) cv. Varuna. Methods: Plants were grown in earthen pots and were exposed to 100 mM NaCl. Further, 30 days old plants were sprayed with 24-epibrassinolide (EBL, 10−6 M) and sodium nitroprusside (SNP, 100 μM) solutions in alone and/or combination. Plant growth indices, gas exchange parameters, chlorophyll fluorescence, nitrogen and proline metabolism, malondialdehyde, electrolytic leakage and ABA content were analyzed. Results: Salinity stress hampered the general plant growth, affected gas exchange parameters, chlorophyll fluorescence and nitrogen metabolism, showed increase in MDA, proline and ABA content while decreased electrolytic leakage and K+/Na+ ratio. The endogenous levels of ABA revealed significant increase in salinity treatment (12.25 μg g−1 FW) and approaching control value (4.31 μg g−1 FW) in combined EBL and SNP treatment. Conclusion: The present findings demonstrated that EBL and SNP application could protect the plants against salinity stress suggesting the involvement of nitric oxide and brassinosteroids in salinity stress amelioration through impact on nitrogen, proline and ABA metabolism. © 2016, Springer International Publishing Switzerland.
Acquired dorsal intraspinal epidermoid cyst in an adult female
(Medknow Publications, 2016) Kulwant Singh; Sharad Pandey; Praveen Gupta; Vivek Sharma; Deepa Santhosh; Amrita Ghosh
Background: Epidermoid and dermoid cyst comprise <1% of spinal tumors and may be congenital (hamartoma) or acquired (iatrogenic) in origin. Epidermoid cysts within the neuraxis are rare benign neoplasms that are most commonly located in the intracranial region. Case Description: Here, we report the a case of an acquired intradural extramedullary epidermoid cyst involving the thoracic region in an adult female who had no associated history of an accompanying congenital spinal deformity. Conclusion: Early diagnosis and immediate surgical intervention reduce patient morbidity. Near complete or subtotal excision of the cyst wall is warranted to prevent inadvertent injury to the spinal cord thus minimizing neurological morbidity. © 2016 Surgical Neurology International.
Nitric oxide mediated regulation of ascorbate-glutathione pathway alleviates mitotic aberrations and DNA damage in Allium cepa L. under salinity stress
(Taylor and Francis Ltd., 2023) Priyanka Prajapati; Praveen Gupta; Ravindra Nath Kharwar; Chandra Shekhar Seth
Allium cepa L. is an important medicinal and food plant enormously affected by salinity in terms of its growth and quality. This experiment investigates ameliorative potential of NO donor sodium nitroprusside (SNP) on chromosomal aberrations and physiological parameters in A. cepa L. roots exposed to salinity stress. Roots with different concentrations of NaCl (25, 50, and 100 mM) alone, and in combination with 100 µM SNP were analyzed for mitotic aberrations, DNA damage, proline, malondialdehyde (MDA) content, and ascorbate-glutathione (AsA-GSH) cycle after 120 h of salinity treatments. Results revealed that salinity stress increased chromosomal aberrations, MDA, proline accumulation, and severely hampered the AsA-GSH cycle function. The comet assay revealed a significant (p ≤ 0.05) enhancement in tail length (4.35 ± 0.05 µm) and olive tail moment (3.19 ± 0.04 µm) at 100 mM NaCl exposure. However, SNP supplementation decreased total percent abnormalities, while increased the prophase, metaphase, anaphase, and telophase indexes. Moreover, ascorbate peroxidase and glutathione reductase activities increased with AsA/DHA and GSH/GSSG ratios, respectively. Results suggest that SNP supplementation alleviates salinity stress responses by improving AsA-GSH cycle and proline accumulation. Based on present findings, NO supplementation could be recommended as a promising approach for sustainable crop production under salinity stress. © 2022 Taylor & Francis Group, LLC.