Browsing by Author "Pritee Chaudhary"

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Association of oxidative stress and endothelial dysfunction in hypertension
(Academic Press Inc., 2020) Pritee Chaudhary; Anand Pandey; Chandra Shekhar Azad; Neelam Tia; Manish Singh; Indrajeet Singh Gambhir
Background: Biomarkers of oxidative stress (OS) are involved in the pathophysiology of hypertension (HTN) and endothelial dysfunction is also related to HTN. Still, a significant association of OS, as well as endothelial function, remains unclear in HTN. Methods: Totalling 222 North Indian peoples aged 18–80 participated in the study. Of these participants, 74 were elderly hypertensive subjects (age ≥60 years), and 128 were normotensive subjects (age ≥60 years-control I; n = 74, and <60 years-control II; n = 74). OS was assessed by measurement of total oxidant status (TOS) and total antioxidant status (TAS) using a colorimetric and automated method developed by Erel O. Endothelial dysfunction was assessed by measurement of flow-mediated dilation (FMD) using doppler ultrasound system. Results: TOS and OSI were significantly increased and TAS and FMD significantly decreased in patients with HTN as compared to control I and control II. The increase in the level of TOS and a decrease in the level of TAS and FMD were also evident with advancing age. FMD was negatively correlated with TOS and positively correlated with TAS. Conclusion: Decreased TAS level, increased TOS level reflect OS that may be the reason for reduced FMD in elderly hypertensive patients. © 2019 Elsevier Inc.
Lipid-Based Nanocarriers for Brain Delivery of Drugs in Neurodegenerative Disorders
(CRC Press, 2024) Mukesh Kumar Yadav; Manish Singh; Pritee Chaudhary; Shardendu Kumar Mishra
The central nervous system (CNS) is vulnerable to neurodegenerative disorders like Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and multiple sclerosis. These are chronic and progressive pathologies characterized by the loss of neurons and the formation of misfolded proteins, for example, α-synuclein, β- amyloid. A neurodegenerative disease is characterized by symptoms such as memory loss, moodiness, anxiety, depression, and agitation. Neurodegeneration involves structural and functional dysfunction of the blood-brain barrier (BBB) that acts as a shield to precent blood toxins from accessing the brain. With the focus on drug discovery and research for targeted drug therapy to the CNS, advancements in therapeutics and medicine have allowed for a better understanding of the many pathways involved in the emergence of neurodegenerative diseases. In this situation, nanotechnology methods have emerged as a viable tactic to enhance drug targeting to the brain and to boost bioavailability. Nanocarrier drug delivery for neurodegenerative diseases opens the door for promising approaches and advances in the diagnosis and treatment of various neurodegenerative diseases. Lipid-based nanocarriers, categorized as bilayered vesicular drug-delivery systems; unilayered lipid drug-delivery systems; and submicron emulsions have been developed to overcome shortcomings related to CNS delivery of therapeutic molecules. Over the years, various studies have used the application of lipid-based nanocarriers for the treatment of different neurological conditions. This chapter comprises knowledge about different neurodegenerative disorders, the role of the BBB, the development of lipid-based nanocarriers, and their application of drug-delivery systems in the treatment of neurodegenerative disorders. © 2024 selection and editorial matter, Anurag Kumar Singh, Vivek K. Chaturvedi, and Jay Singh; individual chapters, the contributors.
Pharmacokinetic screening to estimate the drug likeliness characteristics of selected herbal anticancer drugs
(Research Journal of Pharmacy and Technology, 2023) Manish Singh; Brijesh Kumar; Alok K Singh; Chandra Shekhar Azad; Mukesh K Yadav; Ashutosh Kumar; Rajesh Kumar; Ajay Kumar; Pritee Chaudhary
The pharmacokinetic parameters of a drug plays a very essential role in determining the therapeutic success of an experimental compound, so it is one of the aspects of drug discovery which are essential to be determined in the early phases. The pharmacokinetic studies further help the drug discovery team to optimize their in vivo pharmacokinetic and drug safety bioassays.Low solubility, low absorbency, and chemical instability can seriously affect bioassay results. Today a lot of computational software are available which use their algorithms to calculate the pharmacokinetic parameters of the selected compounds and hence may help the drug discovery team to move in a direction where the chances of getting a good clinical candidate are higher. This paper presents the screening of nine selected herbal anticancer agents (Catechin, Cinnamaldehyde, Epicatechin, Eugenol, Oxyresveratrol, Quercetin, Crocin, Kaempferol, and Emodin) based upon their pharmacokinetic properties with the help of Discovery Studio 2.5. The main parameters which are estimated under this pharmacokinetic ADMET (absorption, distribution, metabolism, excretion and toxicity) study are aqueous solubility, human intestinal absorption, plasma protein binding (PPB), blood-brain-barrier (BBB) penetration, cytochrome P4502D6 inhibition and hepatotoxicity levels. Four compounds (Cinnamaldehyde, Eugenol, Crocin and Oxyresveratrol) were found to possess the required pharmacokinetic properties and are suitable for further anticancer in vivo and in vitro analysis. © RJPT All right reserved.
Role of Forkhead Box O (FOXO) transcription factor in aging and diseases
(Elsevier B.V., 2018) Neelam Tia; Alok Kumar Singh; Poorti Pandey; Chandra Shekhar Azad; Pritee Chaudhary; Indrajeet Singh Gambhir
Fork head box O (FOXO) transcription factor is a key player in an evolutionarily conserved pathway. The mammalian FOXO family consists of FOXO1, 3, 4 and 6, are highly similar in their structure, function and regulation. To maintain optimum body function, the organisms have developed complex mechanisms for homeostasis. Importantly, it is well known that when these mechanisms dysregulate it results in the development of age-related disease. FOXO proteins are involved in a diverse cellular function and also have clinical significance including cell cycle arrest, cell differentiation, tumour suppression, DNA repair, longevity, diabetic complications, immunity, wound healing, regulation of metabolism and thus treatment of several types of diseases. By the combinations of post-translational modifications FOXO's serve as a ‘molecular code’ to sense external stimuli and recruit it as to specific regions of the genome and provide an integrated cellular response to changing physiological conditions. Akt/Protein kinase B a signaling pathway as a main regulator of FOXO to perform a diverse function in organisms. The present review summarizes the molecular and clinical aspects of FOXO transcription factor. And also elaborate the interaction of FOXO with the nucleosome remodelling complex to target genes, which is essential to cellular homeostasis. © 2017