Browsing by Author "Priya Shree"

Now showing 1 - 8 of 8

Active phytochemicals of Pueraria tuberosa for DPP-IV inhibition: In silico and experimental approach
(BioMed Central Ltd., 2017) Shivani Srivastava; Priya Shree; Yamini Bhusan Tripathi
Background: We had earlier reported that the extract of Pueraria tuberosa significantly inhibits DPP-IV enzyme, resulting in glucose tolerance response in rats. In this study, we have explored the active phytochemicals responsible for this potential. The results have been validated in both fasting and postprandial states in the plasma of normal rats and also in fasting blood and intestinal homogenates of diabetic models. Methods: Pueraria tuberosa water extract (PTWE) was administered to normal Charles Foster rats for 35days and to diabetic model (65mg/kg bw) for 10days. After treatments, oral glucose tolerance test (OGTT) and insulin was done for 90min, and the changes in the levels of GLP-1, GIP, and DPP-IV activities were monitored in fasting and postprandial states. In the case of the diabetic model, DPP-IV activity was measured in intestinal homogenate and basal insulin in plasma. The components of PTWE were analyzed via HPLC-MS based on their chemical formula, molecular mass, and retention time. Using the molecular docking study, we have selected the top five components having strong binding energy with DPP-IV. Results: The increase in secretion of GLP-1 and GIP was significantly higher in the postprandial state when compared to fasting condition. GLP-1 plasma concentration increased by 5.8 and 2.9 folds and GIP increased by 8.7 and 2.4 folds in PTWE and control rats, respectively. In contrast, the postprandial decrease in DPP-IV specific activities was recorded at 2.3 and 1.4 folds. The response in OGTT and insulin was also consistent with these changes. In comparison to diabetic controls, PTWE-administered rats showed decreased DPP-IV activity in the intestine, leading to enhanced basal insulin concentration. Through molecular docking, we found Puerarone and Robinin to be the most potential phytochemicals of PTWE for DPP-IV inhibition. Binding energy (kcal/mol) and dissociation constant (pM) of Robinin with DPP-IV protein were found to be 7.543 and 2,957,383.75, respectively. For Puerarone, it was 7.376 and 3,920,309, respectively. Conclusions: Thus, this study provides the novel active components that contribute to the DPP-IV inhibitory property of PTWE. © 2017 The Author(s).
Corrigendum to “Incretin hormones receptor signaling plays the key role in antidiabetic potential of PTY-2 against STZ-induced pancreatitis” [Biomed. Pharmacother. 97 (330–338) 330–338] (Biomedicine & Pharmacotherapy (2018) 97 (330–338), (S0753332217332328) (10.1016/j.biopha.2017.10.071))
(Elsevier Masson SAS, 2018) Shivani Srivastava; Priya Shree; Harsh Pandey; Yamini Bhusan Tripathi
The authors regret the authors would like to change the word “Pancreatitis” to “β cells damage” throughout the manuscript, from heading to conclusion. The authors would like to change the third sentence of introduction “Thus, for diabetes progression, it is necessary to prevent the rate of pancreatitis.” to “Thus, for diabetes prevention, it is necessary to reduce the rate of β cells damage.” The authors would like to change the unit of glucose in Animal Design “200 g/dL” to 200 mg/dL.” The authors would like to change the name of secondary antibodies “anti-mouse-AF546 and anti-rabbit-AF488” to “anti-rabbit AF 546 (Red) and anti-mouse AF 488 (Green)” The authors would like to change the last sentence of discussion “All these changes collectively inhibit the apoptosis of pancreatic β cells through downregulation of Bcl 2 and improved insulin synthesis” to “All these changes collectively inhibit the apoptosis of pancreatic β cells through upregulation of Bcl 2 and improved insulin synthesis” The authors would like to apologise for any inconvenience caused. © 2017 Elsevier Masson SAS
In silico screening of Pueraria tuberosa (PTY-2) for targeting COVID-19 by countering dual targets Mpro and TMPRSS2
(Taylor and Francis Ltd., 2022) Priya Shree; Priyanka Mishra; Prateek Kumar; Harsh Pandey; Rajanish Giri; Radha Chaube; Neha Garg; Yamini Bhusan Tripathi
COVID-19 pandemic was started in Wuhan city of China in December 2019; immensely affected global population. Herein, an effort was made to identify potential inhibitors from active phytochemicals of Pueraria tuberosa (PTY-2) via molecular docking study. Our study showed five potential inhibitors (Robinin, Genistin, Daidzin, Hydroxytuberosone, Tuberostan) against Mpro and five inhibitors (Robinin, Anhydrotuberosin, Daidzin, Hydroxytuberosone, Stigmasterol) against TMPRSS2. Out of these, Robinin, Daidzin and Hydroxytuberosone were common inhibitors for Mpro and TMPRSS2. Among these, Robinin showed the highest binding affinity, therefore, tested for MD simulation runs and found stable. ADMET analysis revealed the best-docked compounds are safe and follow the Lipinski Rule of Five. Thus, it could be suggested that phytochemicals of PTY-2 could serve as potential inhibitors for COVID-19 targets. Communicated by Ramaswamy H. Sarma. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Incretin hormones receptor signaling plays the key role in antidiabetic potential of PTY-2 against STZ-induced pancreatitis
(Elsevier Masson SAS, 2018) Shivani Srivastava; Priya Shree; Harsh Pandey; Yamini Bhusan Tripathi
Aims Incretin therapy is one of the most potential approaches in the treatment of diabetes. In contrast to markedly available drugs, the herbal incretin modulators have lesser side effects with low economic cost. The main aim of this work was to analyze the potential of previously reported DPPIV inhibitor, aqueous extract of Pueraria tuberosa tubers (PTY-2) as incretin hormones receptor agonist against streptozotocin (STZ)-induced diabetes. Methods Chronic diabetes was induced with STZ (65 mg/kg bw) in rats for 60 days and grouped into diabetic control and PTY-2. Expression of genes was assessed by PCR, IHC, and ELISA. Morphological analysis of tissue was observed using H & E stain. In silico molecular docking approach has been used to see the interaction of active phytochemicals of PTY-2 on the basis of their binding energy [kcal/mol] and dissociation constant [pM] using YASARA software. Interactive visualization was done using Discovery studio 3.0. Results In comparison to diabetic control, the size and number of islet cells along with the plasma level of GLP-1, GIP, and pancreatic expressions of GLP-1R, GIP-R, Bcl2, and insulin were enhanced significantly after PTY-2 treatment. Through in silico molecular docking, tuberostan showed the best interaction for GLP-1R with binding energy at 8.15 kcal/mol and dissociation constant at 1061624.125 pM. Puererone showed the best interaction for GIP-R with binding energy at 8.31 kcal/mol and dissociation constant at 810381 pM. Conclusions In addition to previously studied DPPIV inhibitor, PTY-2 also acts as incretin receptors agonist and protects against STZ-induced diabetes by down regulating β cells apoptosis. © 2017 Elsevier Masson SAS
Phytonutrient Inhibitors of SARS-CoV-2/NSP5-Encoded Main Protease (Mpro) Autocleavage Enzyme Critical for COVID-19 Pathogenesis
(Taylor and Francis Ltd., 2023) Sreus A. G. Naidu; Yamini B. Tripathi; Priya Shree; Roger A. Clemens; A. Satyanarayan Naidu
The genomic reshuffling, mutagenicity, and high transmission rate of the SARS-CoV-2 pathogen highlights an urgent need for effective antiviral interventions for COVID-19 control. Targeting the highly conserved viral genes and/or gene-encoded viral proteins such as main proteinase (Mpro), RNA-dependent RNA polymerase (RdRp) and helicases are plausible antiviral approaches to prevent replication and propagation of the SARS-CoV-2 infection. Coronaviruses (CoVs) are prone to extensive mutagenesis; however, any genetic alteration to its highly conserved Mpro enzyme is often detrimental to the viral pathogen. Therefore, inhibitors that target the Mpro enzyme could reduce the risk of mutation-mediated drug resistance and provide effective antiviral protection. Several existing antiviral drugs and dietary bioactives are currently repurposed to treat COVID-19. Dietary bioactives from three ayurvedic medicinal herbs, 18 β-glycyrrhetinic acid (ΔG = 8.86 kcal/mol), Solanocapsine (ΔG = 8.59 kcal/mol), and Vasicoline (ΔG = 7.34 kcal/mol), showed high-affinity binding to Mpro enzyme than the native N3 inhibitor (ΔG = 5.41 kcal/mol). Flavonoids strongly inhibited SARS-CoV-2 Mpro with comparable or higher potency than the antiviral drug, remdesivir. Several tannin hydrolysates avidly bound to the receptor-binding domain and catalytic dyad (His41 and Cys145) of SARS‐CoV‐2 Mpro through H-bonding forces. Quercetin binding to Mpro altered the thermostability of the viral protein through redox-based mechanism and inhibited the viral enzymatic activity. Interaction of quercetin-derivatives with the Mpro seem to be influenced by the 7-OH group and the acetoxylation of sugar moiety on the ligand molecule. Based on pharmacokinetic and ADMET profiles, several phytonutrients could serve as a promising redox nutraceutical for COVID-19 management. © 2021 Taylor & Francis Group, LLC.
Saliva Based Diagnostic Prediction of Oral Squamous Cell Carcinoma using FTIR Spectroscopy
(Springer, 2024) Priya Shree; Yogendra Aggarwal; Manish Kumar; Lakhan Majhee; Narendra Nath Singh; Om Prakash; Akhilesh Chandra; Simpy Amit Mahuli; Shoa Shamsi; Arpita Rai
Oral cancer ranks as the sixth most prevalent form of cancer worldwide, presenting a significant public health concern. According to the World Health Organization (WHO), within a 5-year period following diagnosis, the mortality rate among oral cancer patients of all stages stands at 45%. In this study, a total of 60 patients divided into 2 groups were recruited. Group A included 30 histo-pathologically confirmed OSCC patients and Group B included 30 healthy controls. A standardized procedure was followed to collect saliva samples. FTIR spectroscopy was done for all the saliva samples collected from both Group A and B. An IR Prestige-21 (Shimadzu Corp, Japan) spectrometer was used to record IR spectra in the 40–4000 cm−1 range SVM classifier was applied in the classification of disease state from normal subjects using FTIR data. The peaks were identified at wave no 1180 cm−1, 1230 cm−1, 1340 cm−1, 1360 cm−1, 1420 cm−1, 1460 cm−1, 1500 cm−1, 1540 cm−1, 1560 cm−1, and 1637 cm−1. The observed results of SVM demonstrated the accuracy of 91.66% in the classification of Cancer tissues from the normal subjects with sensitivity of 83.33% while specificity and precision of 100.0%. The development of oral cancer leads to noticeable alterations in the secondary structure of proteins. These findings emphasize the promising use of ATR-FTIR platforms in conjunction with machine learning as a reliable, non-invasive, reagent-free, and highly sensitive method for screening and monitoring individuals with oral cancer. © Association of Otolaryngologists of India 2024.
Salivary transforming growth factor beta in oral submucous fibrosis: A diagnostic and predictive marker
(Wolters Kluwer Medknow Publications, 2024) Arpita Rai; Shama Parveen; Priya Shree; Tanveer Ahmed; Sher Ali; Mandeep Kaur; Keya Sircar; Debora Sybil; Akhilesh Chandra
Context: Growth factors and cytokines like transforming growth factor beta (TGF-b) play a key role in the pathogenesis of oral submucous fibrosis. Aims: To elucidate the role of Salivary TGF-b isoforms as a predictive and diagnostic marker for oral submucous fibrosis. Settings and Design: A total of 30 OSMF and 10 control patients were included in this study, and their clinic-epidemiological data was recorded. Methodology: The expression of TGF-b genes—TGF-b1, TGF-b2, TGF-b3—was studied by a real-time polymerase chain reaction in tissue and saliva. Patients were given medicinal intervention for 12 weeks along with jaw-opening exercises. Expression of salivary TGF-b genes was studied at 12 weeks. Statistical Analysis Used: SPSS software version 20. Result: Expression of salivary TGF beta isoforms in OSMF was more than in the control group. There was an increase in salivary TGF-b1, b2, b3 expressions with increasing clinical grades of OSMF and advancing the stage of the disease. Expression of all the TGF beta isoforms was decreased after treatment with statistically significant results. Statistically significant correlations were found between the mean difference of TGF-b1 and the mean difference between mouth opening and tongue protrusion. Conclusion: Salivary TGF-b isoforms may be used in diagnosis, risk assessment, and screening of the entire population at risk of OSMF after its clinical validation. However, adequate sample size and segmental assessment of the expression of TGF-b isoforms are needed for further evaluation. © 2023 Journal of Cancer Research and Therapeutics.
Targeting COVID-19 (SARS-CoV-2) main protease through active phytochemicals of ayurvedic medicinal plants–Withania somnifera (Ashwagandha), Tinospora cordifolia (Giloy) and Ocimum sanctum (Tulsi)–a molecular docking study
(Taylor and Francis Ltd., 2022) Priya Shree; Priyanka Mishra; Chandrabose Selvaraj; Sanjeev Kumar Singh; Radha Chaube; Neha Garg; Yamini Bhusan Tripathi
COVID-19 (Coronavirus disease 2019) is a transmissible disease initiated and propagated through a new virus strain SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) since 31st December 2019 in Wuhan city of China and the infection has outspread globally influencing millions of people. Here, an attempt was made to recognize natural phytochemicals from medicinal plants, in order to reutilize them against COVID-19 by the virtue of molecular docking and molecular dynamics (MD) simulation study. Molecular docking study showed six probable inhibitors against SARS-CoV-2 Mpro (Main protease), two from Withania somnifera (Ashwagandha) (Withanoside V [10.32 kcal/mol] and Somniferine [9.62 kcal/mol]), one from Tinospora cordifolia (Giloy) (Tinocordiside [8.10 kcal/mol]) and three from Ocimum sanctum (Tulsi) (Vicenin [8.97 kcal/mol], Isorientin 4′-O-glucoside 2″-O-p-hydroxybenzoagte [8.55 kcal/mol] and Ursolic acid [8.52 kcal/mol]). ADMET profile prediction showed that the best docked phytochemicals from present work were safe and possesses drug-like properties. Further MD simulation study was performed to assess the constancy of docked complexes and found stable. Hence from present study it could be suggested that active phytochemicals from medicinal plants could potentially inhibit Mpro of SARS-CoV-2 and further equip the management strategy against COVID-19-a global contagion. Highlights Holistic approach of Ayurvedic medicinal plants to avenge against COVID-19 pandemic. Active phytoconstituents of Ayurvedic medicinal plants Withania somnifera (Ashwagandha), Tinospora cordifolia (Giloy) and Ocimum sanctum (Tulsi) predicted to significantly hinder main protease (Mpro or 3Clpro) of SARS-CoV-2. Through molecular docking and molecular dynamic simulation study, Withanoside V, Somniferine, Tinocordiside, Vicenin, Ursolic acid and Isorientin 4′-O-glucoside 2″-O-p-hydroxybenzoagte were anticipated to impede the activity of SARS-CoV-2 Mpro. Drug-likeness and ADMET profile prediction of best docked compounds from present study were predicted to be safe, drug-like compounds with no toxicity. Communicated by Ramaswamy H. Sarma. © 2020 Informa UK Limited, trading as Taylor & Francis Group.