Browsing by Author "Priya Singh"

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A Chemodosimeter Exhibiting Fluorescence “Turn-On” Response to Detect Copper(II) Ions: Cell Imaging and Logic Function
(Wiley-Blackwell, 2019) Ramesh C. Gupta; Sushil K. Dwivedi; Syed S. Razi; Priya Singh; Biplob Koch; Arvind Misra
In the present work the photophysical properties of a chemodosimeter, 2-[4’-(N,N’-dimethylamino-biphenyl)-4-methylene]-iminophenol, 5 has been explored to recognize Cu 2+ ion selectively through naked eye sensitive fluorescence “turn-on” response. The observed fluorescence enhancement (∼58 fold) and rise in quantum yield (34%) with rapid response time is attributed to the hydrolysis of aldimine function in the presence of copper. The cell imaging studies showed that chemodosimeter, 5 is well-suited to detect Cu 2+ intracellularly. Additionally, the “Off–On” switching behavior of 5 has been utilized to mimic the function of a sequential logic circuit at molecular level. © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
A dihydrazone based “turn–on” fluorescent probe for selective determination of Al3+ ions in aqueous ethanol
(Elsevier B.V., 2017) Divya Pratap Singh; Romi Dwivedi; Ashish Kumar Singh; Biplob Koch; Priya Singh; Vinod Prasad Singh
An efficient and highly selective dihydrazone based fluorescent probe N′,N′–bis((2–hydroxynaphthalen–1–yl)methylene)malonohydrazide (H2nmh), has been synthesized for selective detection of Al3+ ions and characterized by different physico–chemical and spectroscopic techniques. The probe shows an enhanced fluorescence in the presence of Al3+ ions in ethanol–water (2:3 v/v) solution which is not observed in the presence of other cations (Na+, K+, Mg2+, Ca2+, Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Pb2+, Cd2+ and Hg2+). The binding modes of H2nmh with Al3+ were studied by UV–vis, fluorescence and 1H NMR titrations. The probe act as dibasic hexa–dentate ligand and interacts with two Al3+ ions with a binding constant KB = 5.74 × 109 M−1 and detection limit 5.78 × 10−8 M. Detailed insights of probe–metal interaction mechanism were studied by mean of density functional theory (DFT) as well as time dependent–DFT calculation. MTT assay on live MCF–7 cells has been performed to evaluate the cytotoxicity of the probe which suggests viability of the probe to MCF–7 cells even at higher concentration (100 μM) with no serious cytotoxicity in cells. Live cell imaging study clearly indicates that the accumulation of Al3+ in the cytoplasm of cells can be detected by H2nmh. © 2016 Elsevier B.V.
A fluorescent Ce-EDTA probe for the sensing of ascorbic acid and lysine in real samples
(Royal Society of Chemistry, 2022) None Rajpal; Ashish Kumar; Subhajit Jana; Priya Singh; Rajiv Prakash
In the present work, a fluorescent probe based on an aqueous dispersion of coordination complex Ce-EDTA is utilized for sensing carboxyl compounds. Ce-EDTA is synthesized herein by a hydrothermal method with the help of stoichiometric ratios of cerium (Ce) (+iii) salts and green ligand ethylenediamine N,N,N,N tetra-acetic acid (EDTA). In Ce-EDTA, the Ce ion is surrounded by hexadentate EDTA and three labile aqua ligands, evidenced by FT-IR, XPS, and UV-visible spectroscopy. It exists in the form of polygonal microplates, as apparent by SEM. The sensing mechanism is proposed by choosing carboxyl groups as a part of the ring and open chain. Under the optimized situations, the fluorescence intensities of Ce-EDTA are monitored towards various concentrations of l-lysine (Lys) and ascorbic acid (AA) in these regards. They exhibit a linear response from 20.29 μM to 251.74 μM and 5.62 μM to 60.87 μM with a linear correlation coefficient (R2) of 0.98 and 0.99, respectively. The lower limit of detection is observed as 3.6 μM in the case of Lys and 2.04 μM in the case of AA. The as-prepared probe exhibits suitability for selectivity of AA and Lys in the presence of various interfering agents as well as in real samples for the detection of AA in orange and Lys in soybean seed. © 2022 RSC.
A study on analytical techniques and statistical design for tertiary treatment of secondary wastewater using Zetag-4120 coagulant
(Indian Academy of Sciences, 2024) Priya Singh; Mahendra Kumar Pal; Prabhat Kumar Singh Dikshit; Goutham Banerjee
The primary and secondary wastewater treatments from different sewage treatment plants are of great importance not only for removing pollutants but also for using treated water for different purposes. The latter involves using suitable coagulants and their optimal dosages for the treatment. Given this, the present study identified the most suitable coagulant for tertiary treatment and its optimal dosage to achieve the maximum total suspended solids (TSS) and biological oxygen demand (BOD) removal. We used six coagulants (alum, ferric chloride, polyaluminium chloride (PAC), Zetag-4120, Mecafloc-25 and Rothfloc-27) and found that Zetag-4120 is the cost-efficient coagulant at varying coagulant dosage. We arrived at this conclusion based on several experiments using varying coagulant dosages. Furthermore, the regression prediction models for BOD and TSS removal for coagulant Zetag-4120 have been generated using statistical models and validated by calculating R2 value, F-test and Lack-of-fit test using ANOVA. Optimization analysis suggests the optimal dosage, initial wastewater pH and settling time to be 1.86 mg/l, 6.73 and 89.12 min respectively. Furthermore, BOD and TSS removal efficiency values are 50.0% and 92.4% respectively. The treated wastewater has BOD and TSS equal to 12.27 and 5.66 mg/l respectively, conforming to the standard for re-usability of treated water for unrestricted irrigation. © (2024), (Indian Academy of Sciences). All rights reserved.
A water soluble, highly sensitive and selective fluorescent probe for Al3+ ions and its application in live cell imaging
(Elsevier B.V., 2017) Shraddha Rani Gupta; Priya Singh; Biplob Koch; Vinod P. Singh
An efficient, aminothiazole based, fluorescent probe (E)-2-(2-aminothiazol-5-yl)-N'-((2-hydroxynaphthalen-1-yl)methylene)acetohydrazide (NTH) for the detection of Al3+ ions was synthesized and characterized by different physico-chemical and spectroscopic tools. An attractive glowing blue color was observed in the presence of Al3+ with single channel emissions for NTH (λem 451 & λex 391 nm). NTH, selectively detected Al3+ ions among various other ions without any significant interference in Tris-HCl buffer solution (10 mM, pH ∼ 7.4). The >C[dbnd]N– isomerization was responsible for the turn ‘on’ fluorescence response after Al3+ binding. The stoichiometry of NTH with Al3+ was determined to be 1:1 by Job's plot. The binding constant and limit of detection (LOD) were observed as 3.65 × 109 M−1 and 1.09 × 10−9 M, respectively. The 1H NMR titration and DFT studies were also performed in support of binding details of NTH-Al3+ complex. MTT assay on live A549 cells suggested viability of the probe to A549 cells even at higher concentration (100 μM) with no serious cytotoxicity in cells. Live cell imaging study clearly indicated that the accumulation of Al3+ in the cytoplasm of cells could be detected by NTH. © 2017 Elsevier B.V.
An efficient Hg2+ ensemble based on a triazole bridged anthracene and quinoline system for selective detection of cyanide through fluorescence turn-off–on response in solution and live cell
(Elsevier B.V., 2017) Ramesh C. Gupta; Syed S. Razi; Rashid Ali; Sushil K. Dwivedi; Priyanka Srivastava; Priya Singh; Biplob Koch; Hirdyesh Mishra; Arvind Misra
A simple triazole bridged molecular probe containing anthracene and quinoline moieties has been designed and synthesized as a fluorescent molecular probe (7). The photophysical behavior of the probe 7 has been examined and tested for the recognition of ions in partial aqueous medium and HeLa cell. The probe 7 has shown strong affinity for Hg2+ and has been utilized to develop a stable ensemble, 7·Hg2+. The ensemble, 7·Hg2+ upon interaction with different class of anions showed high affinity for cyanide with ∼16 fold fluorescence enhancement and high limit of detection, 1.25 μM. The live cell imaging studies showed that ensemble 7·Hg2+ is capable to detect CN− intracellularly. The analytical applicability of the probe for Hg2+ and CN− has been tested on the test paper strips. Additionally, the naked eye sensitive “On–Off–On” switching behavior of 7 has been utilized to mimic the function of a sequential logic circuit at molecular level. The 1H NMR titration, ESI-MS spectroscopy and DFT calculation confirms the binding behavior between 7 and Hg2+. © 2017 Elsevier B.V.
Association between job control and psychological health in middle-level managers
(National Institute of Industrial Health, 2023) Priya Singh; Prabhas Bhardwaj; Susheel K. Sharma
The present study analyses the effect of job control at work on psychological stress for Indian Middle-Level Managers (MLMs) of a public telecom organisation. Two hundred ten MLMs from different parts of India have participated in the survey. Three dimensions of job control visualize control over work (CoW), control over working time (CoT1) and control over working days (CoT2), were considered. The validity and reliability were confirmed using Factor and reliability analysis. A Binary Logistics Regression (BLR) was performed to find the effect of job control on behavioural, somatic and cognitive stress controlling for age, gender, and experience. The Odds Ratio and Adjusted Odds ratio were calculated. 56% of the participants reported suffering from psychological stress. Results showed that CoT1 had a significant association with somatic stress while CoT1 and CoT2 with cognitive stress. Low CoW and low CoT2 were associated with high psychological stress among middle-level managers while low CoT1 to low psychological stress. The findings indicate that job control have both positive and negative relationships with psychological well-being depending on its dimension. Increasing job control cannot entirely ensure the psychological well-being of employees. Therefore, organisations need to assess different dimensions of job control carefully before providing work flexibility to employees. ©2023 National Institute of Occupational Safety and Health.
CoCl2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis
(NLM (Medline), 2019) Nishant Kumar Rana; Priya Singh; Biplob Koch
BACKGROUND/AIMS: Hypoxia microenvironment plays a crucial role during tumor progression and it tends to exhibit poor prognosis and make resistant to various conventional therapies. HIF-1α acts as an important transcriptional regulator directly or indirectly associated with genes involved in cell proliferation, angiogenesis, apoptosis and energy metabolism during tumor progression in hypoxic microenvironment. This study was aimed to investigate the expression pattern of the hypoxia associated genes and their association during breast cancer progression under hypoxic microenvironment in breast cancer cells. METHODS: Cell proliferation in MCF-7 and MDA-MB-231 cell lines treated with different concentration of CoCl2 was analyzed by MTT assay. Flow cytometry was performed to check cell cycle distribution, whereas cell morphology was examined by phase contrast microscopy in both the cells during hypoxia induction. Expression of hypoxia associated genes HIF-1α, VEGF, p53 and BAX were determined by semiquantitative RT-PCR and real-time PCR. Western blotting was performed to detect the expression at protein level. RESULTS: Our study revealed that cell proliferation in CoCl2 treated breast cancer cells were concentration dependent and varies with different cell types, further increase in CoCl2 concentration leads to apoptotic cell death. Further, accumulation of p53 protein in response to hypoxia as compare to normoxia showed that induction of p53 in breast cancer cells is HIF-1α dependent. HIF-1α dependent BAX expression during hypoxia revealed that after certain extent of hypoxia induction, over expression of BAX conquers the effect of anti-apoptotic proteins and ultimately leads to apoptosis in breast cancer cells. CONCLUSION: In conclusion our results clearly indicate that CoCl2 simulated hypoxia induce the accumulation of HIF-1α protein and alter the expression of hypoxia associated genes involved in angiogenesis and apoptosis.
Digital twins and artificial intelligence
(CRC Press, 2024) Priya Singh; Sankata Tiwari
[No abstract available]
Dual Fluorophore Containing Efficient Photoinduced Electron Transfer Based Molecular Probe for Selective Detection of Cr3+ and PO4 3- Ions through Fluorescence " Turn-On-Off" Response in Partial Aqueous and Biological Medium: Live Cell Imaging and Logic Application
(American Chemical Society, 2018) Sushil K. Dwivedi; Ramesh C. Gupta; Priyanka Srivastava; Priya Singh; Biplob Koch; Biswajit Maiti; Arvind Misra
The present work describes a new photoinduced electron transfer (PET) based molecular probe in which naphthalimide (NPI) and anthracene (AN) chromophores are linked through a molecular bridge of piperazine and triazole units by the Click reaction. A typical meaningful structural variation has made the present probe highly selective for Cr3+ ion (limit of detection (LOD), 5.567 × 10-8 M) that displayed enhanced, "turn-On" emission (due to the PET-Off photophysical mechanism) and naked-eye sensitive bright green color fluorescence in the environment of interfering and competitive ions, in Tris-HCl buffer. The minimum energy structure obtained through theoretical calculations (density functional theory (DFT) and time-dependent (TD)-DFT) revealed a "tub" shape structure for probe 10. Upon complexation, the conformation of piperazine fragment changes from chair to boat in which the triazole and piperazine units create a cavity to tether Cr3+. Moreover, the probe showed excellent biocompatibility and cell permeability to sense Cr3+ sensitively in live cells and, thus, holds great promise for application in biological and environmental sciences. Additionally, the sensitive "Off-On-Off" sensing behavior of probe 10 providing two chemical inputs (Cr3+ and PO4 3-) helps to construct an INHIBIT logic gate. Also the probe has been utilized as printing material to decode secret information through the Cr3+ ion containing "marker ink" under UV light. © 2018 American Chemical Society.
Ecotourism: A method for sustainable development in the period of international integration
(Nova Science Publishers, Inc., 2022) Priya Singh
Tourism has been one of the world's fastest-growing business, with sales and volume increasing by 7% yearly. In 2016, tourism accounted for one in every eleven employment and ten percent of global economic output. To be efficient, ecotourism should promote sustainable development by establishing a long-term productive base that benefits both residents and ecotourism providers. It was found in a study conducted on the Monarch Butterfly's wintering grounds in western-central Mexico that ecotourism is an option in contrast to tourism. It is the practice of visiting natural areas to educate, study or participate in ecologically useful things, i.e., tourism based on the natural experience that supports local communities economic and social growth, focused on experiencing and learning about ecology, such as the region's geography, flora, fauna habitats and cultural artifacts. Sustainable development became a potent and divisive topic with policymakers and development practitioners facing unachievable targets within economic, social, cultural, ecological, and physical restrictions. Ecotourism as a means of achieving sustainable development is a serious topic on the modern world stage. Ecotourism is a broad term that encompasses a range of nature-based activities that enable visitors to respect and comprehend the natural and cultural heritage. There are many strategies through which we can increase ecotourism with increasing productivity like adopting agro-ecological techniques. It also implies introducing new activities that build on the community's and region's cultural and resource bases for future development. © 2022 Nova Science Publishers, Inc..
Effect of carbon quantum dots derived from extracts of UV-B-exposed Eclipta alba on alcohol-induced liver cirrhosis in Golden Hamster
(Springer Nature, 2023) Kshama Rai; Kanchan Yadav; Megha Das; Shilpi Chaudhary; Kaustubh Naik; Priya Singh; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Shashi Bhushan Agrawal; Avanish Singh Parmar
The Eclipta alba plant is considered hepatoprotective, owing to its phytoconstituents wedelolactone. In the current study, effect of elevated ultraviolet-B (eUV-B) radiation was investigated on biochemical, phytochemical, and antioxidative enzymatic activities of E. alba (Bhringraj) plant. The UV-B exposure resulted in an increase in oxidative stress, which has caused an imbalance in phytochemical, biochemical constituents, and induced antioxidative enzymatic activities. It was observed that the UV-B exposure promoted wedelolactone yield by 23.64%. Further, the leaf extract of UV-B-exposed plants was used for the synthesis of carbon quantum dots (CQDs) using low cost, one-step hydrothermal technique and its biocompatibility was studied using in vitro MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay on HepG2 liver cell line. It revealed no toxicity in any treatment groups in comparison to the control. Both CQDs and leaf extract were orally administered to the golden hamster suffering from alcohol-induced liver cirrhosis. In the morphometric study, it was clearly observed that a combination of UV-B-exposed leaf extract and synthesized CQDs delivered the best result with maximum recovery of liver tissues. The present study reveals the positive impact of UV-B exposure on the medicinally important plant, increased yield of wedelolactone, and its enhanced hepatoprotective efficacy for the treatment of damaged liver tissues. Graphical Abstract: [Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
Electret-induced antibacterial response of Mg1-xCaxSi1-xZrxO3 (x = 0–0.4) bioceramics
(John Wiley and Sons Inc, 2024) Priya Singh; Ashutosh Kumar Dubey
The potential risk of bacteria-induced prosthetic infection during implantation/post-operative healing raises a serious concern about the success of implant/surgery. In this context, the present study successfully established the antibacterial efficacy of the electret form of Mg1-xCaxSi1-xZrxO3 (x = 0, 0.1, 0.2, 0.3, 0.4 [MCSZO-X]) bioceramics, toward both, Escherichia Coli and Staphylococcus aureus bacteria with reference to those of hydroxyapatite (HAP). MCSZO-X bioceramics were synthesized using a solid-state route. The influence of Ca- and Zr co-doping on the crystallite size of MCSZO-X has been analyzed using X-ray peak profile analyses. The electrets were developed by corona poling of sintered MCSZO-X samples at voltage and temperature of 20 kV and 500°C (30 min), respectively. The charge, stored on the surface of MCSZO-X electret samples was calculated to be 0.253, 0.294, 0.320, 0.173, and 0.161 µC/cm2 via thermally stimulated depolarized current measurement. The positive and negative ends of MCSZO- X electrets exhibited a reduction in the viability of E. coli bacteria by (24%, 25%, 30%, 43%, and 42%) and (29%, 37%, 39%, 51%, and 48%), respectively, in comparison to those of HAP. On the contrary, the viability of S. aureus bacteria has decreased by (26%, 33%, 35%, 46%, and 42%) and (21%, 22%, 27%, 395, and 37%) on the surfaces of the positive and negative ends of MCSZO- X electrets, respectively, with the reference of those of HAP. The mechanism of electret-induced antibacterial activity has been revealed via various assays, such as catalase activity, superoxide production (SOD), protein estimation, and lipid peroxidation (LPO) assays. The positive ends of MCSZO-X electrets demonstrate antibacterial efficacy by means of more reactive oxygen species generation as compared to their negative ends of electrets and uncharged surfaces of MCSZO-X samples, as revealed by SOD, protein estimation, catalase activity, and LPO assays. However, the negative ends of electrets prevent the adhesion of bacterial cells via electrostatic repulsion. © 2024 The American Ceramic Society.
Impact of rice-husk biochar on soil attributes, microbiome interaction and functional traits of radish plants: A smart candidate for soil engineering
(Elsevier B.V., 2024) Kaushik Gautam; Priya Singh; Rajeev Pratap Singh; Anita Singh
This study is based on the application of different doses of rice husk biochar; RHB (1, 5, 10, 15, and 20 t/ha) in wastewater-irrigated soil to observe its impact on the soil qualities and functional attributes of the radish plants. The results showed that the RHB treatments increased the soil pH, electrical conductivity (EC), bulk density (BD), soil organic carbon (SOC), total nitrogen (TN), available phosphorous (AP), enzymatic activities, and microbial biomass in the soil. The soil metagenome analysis at 15 t/ha showed more abundance of microbial communities like Actinobacteria, Acidobacteria, and Nitrospira over to control soil. On the other hand, more significantly it led to a reduction in the availability of Cd, Cr, Ni, Zn, and Cu by 73, 60, 63, 61, and 68 %, respectively maximally at 20 t/ha dose of biochar application. Along with that it also led to the level of the all the toxic heavy metals below their safe limit in the edible part of the radish plants. All the doses of RHB application have resulted improvements in functional attributes of the radish plants by reducing the production of oxidative biomarkers more significantly at 15 and 20 t/ha doses of biochar compared to the control plants. Among all the doses the biomass of edible parts of plant i.e., root was increased and the maximum increment was found at 15 t/ha over to control plants and later on, there was an insignificant difference. The total concentrations of Cd, Cr, Ni, Zn, and Cu were decreased in the root and shoot of the plants in a dose-dependent manner. The correlation biplot also showed that with the amendment of biochar, there was a significant correlation between the soil properties and plant yield. © 2024
Impact of Thirty-Four Years of Fertilization and Manuring on Soil Nutrients Availability, Yield and Quality of Crops in Indo-Gangetic Plains
(Springer Science and Business Media Deutschland GmbH, 2024) Priya Singh; Sunanda Biswas; Nandita Ghoshal; Nirmal De; Divyadarshan Singh
Dryland rainfed agriculture needs an appropriate fertilization strategy to achieve sustainable yield with good soil health. This study was aimed at assessing the impact of long-term fertilization on soil available nutrients, its enzymatic activities, and yield and quality of crops. Depth-wise soil samples were collected from a 34-year-old long-term fertilizer experiment (LTFE) with a rainfed rice-lentil cropping system. Treatments used for comparison were: control, 100% NPK (recommended NPK), 50% NPK, 50% FYM (50% of recommended nitrogen from farmyard manure), 100% FYM, and 50% NPK + FYM. Collected samples were analyzed for the physico-chemical and biological properties of soils along with the quality and yield of crops harvested. Among the treatments, 50%NPK + FYM showed the highest activities of dehydrogenase (DHA) (72.7%), alkaline phosphatase (ALKP) (48%), arylsulphatase (ASP) (92.8%) and urease (URE) (112%) compared with the control at the surface layer. It (NPK + FYM) also enhanced soil macro- (available N, P, K and S) and micro-nutrients (available Zn, Cu, Fe, Mn and B). The use of FYM either alone or in combination with inorganic fertilizer, had significant impacts on uptake of macro- and micro-nutrients by grains and straw of rice. Conjoint use of FYM and inorganic fertilizer also improved grain yield of rice (2038 kg ha-1) and lentil (965 kg ha-1), values of sustainable yield index (SYI), and quality of rice in terms of enrichment of N, P and K (1.34%, 0.37% and 0.24%) in grains. Results also revealed an improved agronomic efficiency (AE) and apparent recovery efficiency (ARE) of N (38.8%), P (33.7%) and K (91.4%) with FYM. With the exception of control, there was a positive apparent N and P balance shown in all the treatments, whereas negative apparent K balance in all except the FYM-treated plots. Application of NPK with FYM improves soil physico-chemical and biological properties, crop productivity and also its quality. It also ensures a steady supply of N, P and K to crops enhancing their use efficiencies. A balanced and conjoint application of inorganic fertilizer and FYM to rainfed rice-lentil cropping system is recommended for upkeeping soil health, improving crop productivity and its quality under Indo-Gangetic Plains. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2024.
Influence of anchoring moieties on new benzimidazole-based Schiff base copper(ii) complexes towards estrogen dependent breast cancer cells
(Royal Society of Chemistry, 2021) Anup Paul; Priya Singh; Maxim L. Kuznetsov; Anirban Karmakar; M.F.C. Guedes da Silva; Biplob Koch; Armando J. L. Pombeiro
Two new benzimidazole Schiff base copper(ii) compounds [Cu(5-CH2PPh3-2-salmethylben)(NO3)(H2O)][BF4]·2/3(H2O)·1/3(MeOH) (1) and [Cu(5-CH2NEt3-2-salmethylben)(Cl)][BF4] (2) were synthesised by mixing 2-(1-methyl-1H-benzo[d]imidazol-2-yl)aniline, (3-formyl-4-hydroxybenzyl)triphenylphosphonium chloride orN,N-diethyl-N-(3-formyl-4-hydroxybenzyl)ethanaminium chloride and Cu(NO3)2·3H2O or CuCl2·2H2O in the presence of tetrafluoroborate in a binary mixture of MeOH : H2O under refluxing conditions. The structures of the compounds were established by elemental analysis, FT-IR, ESI-MS analytical techniques and, for1, by single-crystal X-ray diffraction analysis. Absorption and fluorescence spectroscopic methods were performed to evaluate the calf thymus DNA interactions with the compounds. The calculated binding constants (Kb) of 3.14 × 105M−1for1and 3.20 × 105M−1for2were established. The intercalative DNA binding mode was also verified by molecular docking studies. Both compounds demonstrated a notablein vitrocytotoxic effect against human A-549 (lung carcinoma), MCF-7 (breast cancer) and HeLa (cervical cancer) cancer cell lines. A substantial repressive effect on the proliferation of MCF-7 cells (breast cancer cells) was observed for compound1. The mechanism of action for the effective antiproliferative activity of1has additionally been confirmed by means of various biological studies such as morphological assessment through AO/EB, detection of apoptotic inductionviaHoechst/PI dual staining, flow cytometry for detection of cell cycle arrest, quantitative analysis of apoptotic cells, DNA degradation, generation of reactive oxygen species (ROS) and by apoptotic induction through mitochondrial staining. © The Royal Society of Chemistry 2021.
Integration of Generative AI and Deep Tabular Data Learning Architecture for Heart Attack Prediction
(Springer Science and Business Media Deutschland GmbH, 2024) Priya Singh; Jyoti Singh Kirar
Heart attacks, also known as myocardial infarctions, are currently the top cause of death across all age categories. Through early detection, recent advances in healthcare—particularly in artificial intelligence (AI)—have increased the survival rate of heart attack patients. However, an enormous quantity of high-quality data is necessary for meaningful advancement in AI research for heart attacks. Real-world cardiac attack datasets were used to validate the research findings. A detailed analysis showed that the CTGAN model produced synthetic heart attack data superior to expectations. The TabNet architecture also beat all prior deep-learning and machine-learning classifiers in the classification stage. In conclusion, this study shows the potential of using deep generative models to generate high-quality synthetic data for heart attack research. It emphasizes the superior performance of interpretable TabNet over traditional machine-learning methods in categorizing heart attacks. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
Non-canonical role of Yorkie in the development of Malpighian tubules of Drosophila melanogaster
(Elsevier Inc., 2025) Priya Singh; Saurabh Chand Sagar; Madhu Gwaldas Tapadia
Tissue morphogenesis is a highly coordinated process necessary for correct morphology and function. The Hippo pathway plays a crucial role in regulating the size of organs, and mutation in this pathway can lead to the development of several diseases including tumor. Our study shows that transcriptional coactivator Yorkie, the downstream target of the Hippo pathway, plays a critical role in developing Malpighian tubules in Drosophila. The polycystic phenotype of Malpighian tubules, caused by the loss of the executioner caspase, Drice, results in enhanced expression of Yorkie. Consequently, the genetic reduction of Yorkie in Drice mutants can reduce the polycystic phenotype, reverting it to normal. Yorkie mutation restores actin organization in Drice mutant Malpighian tubules, leading to the proper cellular arrangement. Additionally, improvement in fluid secretion in the Malpighian tubules of Drice mutants was observed when Yorkie levels was reduced. Unexpectedly, Yorkie mutants have greatly elongated Malpighian tubules and its overexpression reduces the size. Collectively, our findings suggest that Yorkie has a non-canonical role in Malpighian tubule development and it genetically interacts with Drice to regulate the development of renal tubule. © 2025
p53 dependent apoptosis and cell cycle delay induced by heteroleptic complexes in human cervical cancer cells
(Elsevier Masson SAS, 2017) Gunjan Sharma; Nishant Kumar Rana; Priya Singh; Pradeep Dubey; Daya Shankar Pandey; Biplob Koch
We previously reported synthesis of novel arene ruthenium (Ru) complexes and evaluated their antitumor activity in murine lymphoma (DL) cells. In this present study we further investigated the mechanism of action of two ruthenium complexes [complex 1 (η6-arene)RuCl(2-pcdpm)] and complex 2 (η6-arene)RuCl(4-mtdpm)] in cervical cancer cell line (HeLa). Our studies demonstrate that anticancer property of these two complexes was due to induction of apoptosis through p53 mediated pathway as well as arrest of cells in G2/M phase of cell cycle. It is worth to note that the complexes did not cause any substantial cytotoxic effect on normal cells. Further in comprehensive studies, apoptosis inducing property of both complexes were established in accordance with array of morphological changes ranging from membrane blebbing to formation of apoptotic bodies and followed by DNA fragmentation assay. Furthermore, Flow cytometry by Annexin V/PI staining delineate that complex 1 and 2 have strident impact to induce apoptosis in HeLa cells. The complex 1 and 2 treated cells show increased level of intracellular ROS generation which was preceded by p53 activation. Apoptosis induced by 1 and 2 was preceded by mitochondrial aggregations which were monitored by mitotracker. In addition flow cytometry analysis showed that both complexes also effectively arrest cells at G2/M phase of cell cycle. Western blot, RT-PCR as well as Real Time analysis were used to further confirm that the complexes induced apoptosis in p53 dependent pathway. Thus, our promising results can contribute to the rational design of novel potential anticancer agents. © 2017 Elsevier Masson SAS
Palladium nanoparticles supported on mesoporous silica microspheres for enzyme-free amperometric detection of H2O2 released from living cells
(Elsevier B.V., 2018) Rupali Gupta; Priya Singh; Vellaichamy Ganesan; Biplob Koch; Pankaj Kumar Rastogi; Dharmendra Kumar Yadav; Piyush Kumar Sonkar
An enzyme-free sensitive hydrogen peroxide (H2O2) amperometric sensor is developed to detect H2O2 released from living cells using palladium nanoparticles supported on sulfonic acid functionalized mesoporous silica microspheres (Pd@SO3H-MSM). It is synthesized by an easy and facile method and is subsequently used for fabrication of an electrochemical sensing scaffold via drop-casting modification of a glassy carbon electrode (represented as GC/Pd@SO3H-MSM). Comprehensive characterizations including transmission electron microscopy, scanning electron microscopy, powder X-ray diffraction, X-ray photoelectron spectroscopy, UV–vis spectrophotometry and electrochemical impedance spectroscopy to confirm the existence and nature of Pd nanoparticles in Pd@SO3H-MSM. GC/Pd@SO3H-MSM electrode demonstrates electrocatalytic activity for H2O2 reduction in phosphate buffer, leading to a sensitive H2O2 amperometric sensor with wide linear range (47.0 nM-1.0 mM), low detection limit (14.0 nM) and high sensitivity (0.36 μA mM−1 cm-2). It exhibits high selectivity, good reproducibility and long-term stability. More importantly, Pd@SO3H-MSM exhibits no toxicity to living cells and based on its remarkable analytical advantages, it is further unswervingly used to execute real-time detection of H2O2 released from living tumor cells and healthy normal cells. Thus Pd@SO3H-MSM acts as promising material for amperometric determination of H2O2 as well as used to accomplish real-time quantitative detection of H2O2 in biological environment. © 2018 Elsevier B.V.