Browsing by Author "Priyanka Pandey"

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A computational system biology approach to construct gene regulatory networks for salinity response in rice (Oryza sativa)
(Indian Council of Agricultural Research, 2015) Samarendra Das; Priyanka Pandey; Anil Rai; Chinmayee Mohapatra
Salinity is one of the most common abiotic stress which limits agricultural crop production. Salinity stress tolerance in rice (Oryza sativa L.) is an important trait controlled by various genes. The mechanism of salinity stress response in rice is quite complex. Modelling and construction of genetic regulatory networks is an important tool and can be used for understanding this underlying mechanism. This paper considers the problem of modeling and construction of Gene Regulatory Networks using Multiple Linear Regression and Singular Value Decomposition approach coupled with a number of computational tools. The gene networks constructed by using this approach satisfied the scale free property of biological networks and such networks can be used to extract valuable information on the transcription factors, which are salt responsive. The gene ontology enrichment analysis of selected nodes is performed. The developed model can also be used for predicting the gene responses under stress condition and the result shows that the model fits well for the given gene expression data in rice. In this paper, we have identified ten target genes and a series of potential transcription factors for each target gene in rice which are highly salt responsive.
[Cu2(en)2(N3)4]n–A new member in the family of copper(II)-azido assemblies: structural and magnetic studies
(Taylor and Francis Ltd., 2017) Priyanka Pandey; Bhagwan Kharediya; Bahjat Elrez; Jean-Pascal Sutter; Sailaja S. Sunkari
A copper(II) complex with ethylene diamine and azide, viz., [Cu2(en)2(N3)4]n has been isolated under self-assembling conditions and characterized both structurally and magnetically in order to probe the role of reactant molar ratios in generating supramolecular assemblies with strong magnetic interactions toward achieving molecule-based magnets as a long-term objective. The title structure with ferromagnetic interactions is an intermediate member in the family of copper-azido assemblies involving ethylene diamine, obtained by systematic modifications in the reactant molar ratios, highlighting the inherent difficulty in controlling the final solid formation and potential of the delicate equilibrium conditions in directing supramolecular structures, which may display interesting structure-correlated properties. The structure is a chain of μ-1,1 bridged dimers of Cu2, connected to Cu1 through μ-1,3 azide bridges, leading to a rail-road arrangement of polymeric chains. Two such parallel chains are further linked to each other through Cu3, which is connected to Cu2′ of the dimer, through asymmetric end-on μ-1,1 azide bridges, resulting in a 2-D network in the unit cell. The magnetic behavior revealed ferromagnetic interactions in Cu(II) pairs and is dominated by overall antiferromagnetic interactions within the network. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Discrete and one dimensional extended Mn(III) Schiff base complexes with pseudohalides: Synthesis, structure and magnetic studies
(Elsevier S.A., 2019) Priyanka Pandey; Shalini Tripathi; Maheswaran Shanmugam; R.J. Butcher; Sailaja S. Sunkari
Slight variations in the Schiff base ligand and the linker length, led to the isolation of three different Mn(III) complexes under similar synthetic conditions that were characterized structurally and magnetically. [MnIII(L1)(N3)(H2O)]∙DMSO (1) and [MnIII(L2)N(CN)2(CH3OH)]∙CHCl3 (3) are azide and dicyanamide based monomers of Mn(III) with salophen and naphthophen as ligands respectively while [MnIII(L1)N(CN)2]n·H2O (2) is a dicyanamide based 1D-extended structure of Mn(III) with salophen ligand. Magnetic studies on 2 reveal antiferromagnetic interactions between the magnetic centers (J1 = −0.49 cm−1). All the three complexes have strong hydrogen bonding interactions connecting the monomeric units or polymeric chains into 2-D assemblies. The presence of supramolecular interactions within 1–3 was analyzed using Hirshfeld method. © 2019 Elsevier B.V.
Drought Exposed Burkholderia seminalis JRBHU6 Exhibits Antimicrobial Potential Through Pyrazine-1,4-Dione Derivatives Targeting Multiple Bacterial and Fungal Proteins
(Frontiers Media S.A., 2021) Jay Kishor Prasad; Priyanka Pandey; Richa Anand; Richa Raghuwanshi
The present study aimed to explore the antimicrobial potentials of soil bacteria and identify the bioactive compounds and their likely targets through in silico studies. A total 53 bacterial isolates were screened for their antimicrobial potential of which the strain JRBHU6 showing highest antimicrobial activity was identified as Burkholderia seminalis (GenBank accession no. MK500868) based on 16S ribosomal RNA (rRNA) gene sequencing and phylogenetic analysis. B. seminalis JRBHU6 also produced hydrolytic enzymes chitinases and cellulase of significance in accrediting its antimicrobial nature. The bioactive metabolites produced by the isolate were extracted in different organic solvents among which methanolic extract showed best growth-suppressing activities toward multidrug resistant Staphylococcus aureus and fungal strains, viz Fusarium oxysporum, Aspergillus niger, Microsporum gypseum, Trichophyton mentagrophytes, and Trichoderma harzianum. The antimicrobial compounds were purified using silica gel thin layer chromatography and high-performance liquid chromatography (HPLC). On the basis of spectroscopic analysis, the bioactive metabolites were identified as pyrrolo(1,2-a)pyrazine-1,4-dione,hexahydro (PPDH) and pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3(2-methylpropyl) (PPDHMP). In silico molecular docking studies showed the bioactive compounds targeting fungal and bacterial proteins, among which PPDHMP was multitargeting in nature as reported for the first time through this study. © Copyright © 2021 Prasad, Pandey, Anand and Raghuwanshi.
Exploring Drought Tolerance in PGPR-Protected Wheat Plants Through Physiological and Molecular Expression
(Springer, 2025) Jay Kishor Prasad; Riddha Dey; Priyanka Pandey; Richa Raghuwanshi
Osmotic stress exposure adversely affects wheat growth leading to early maturity along with compromised yield. The adverse effects could be partially overcome by seed biopriming with potential PGPR strains Pseudomonas azotoformans JRBHU5, Burkholderia seminalis JRBHU6, and consortia (Pseudomonas azotoformans JRBHU5 + Enterobacter hormaechei JRBHU9) isolated during the study. PGPR with good IAA, EPS, and ACC-D producing capabilities performed better than other bacterial strains. Inoculation of PGPR in wheat plants exposed to osmotic stress significantly strengthened the proline and lignin contents and antioxidant defense system to protect wheat plants against oxidative stress. To combat the oxidative and osmotic stress that frequently occurs during water shortage, PGPR enhances the plants RWC by maintaining soil moisture in the rhizosphere of inoculated plants. The selected bacterial strains were good exopolysaccharide producers which formed a thick biofilm over the root surface helpful in maintaining moisture even under drought. Among all the PGPR strains tested JRBHU5 was most effective in inducing osmotic tolerance in wheat bringing yields comparable to the irrigated control plants. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Ligand directed structural diversity and magnetism in copper(II)-azido assemblies with isomeric aminopyridines: Synthesis, structure, magnetism and theoretical studies
(Royal Society of Chemistry, 2017) Priyanka Pandey; Bhagwan Kharediya; Bahjat Elrez; J.-P. Sutter; G. Bhargavi; M.V. Rajasekharan; Sailaja S. Sunkari
Four new copper complexes, viz. [{Cu(2-aminopyridine)(N3)2(H2O)}2]n (1), [Cu3(3-aminopyridine)2(N3)6]n (2), [{Cu(3-aminopyridine)(N3)2}2]n (3), and [Cu(4-aminopyridine)2(N3)2]n (4), have been synthesized with isomeric aminopyridines, viz. 2-aminopyridine (2-ap), 3-aminopyridine (3-ap), and 4-aminopyridine (4-ap), to probe the role of ligand and reactant molar ratios in directing the polynuclear assemblage and the associated magnetic properties. Ligand geometry is quite influential as can be seen through the versatile structures formed, viz. a hydrogen bonded layer of μ-1,1 azide bridged Cu dimers in 1; a network of two different types of dimers (Cu1-Cu2 & Cu3-Cu3′) involving μ-1,1; μ-1,3; μ-1,1,3; & μ-1,1,3,3 azide bridges in 2; a ladder structure in which μ-1,1 azide bridges form the rungs and μ-1,3 azide bridges form the rails of the ladder in 3; and a 1-D polymer chain involving μ-1,1 azide bridges in 4. Consistent with the bridge geometry, compounds 1 & 2 display ferromagnetic interactions, while 3 & 4 display antiferromagnetic interactions. The rather unexpected antiferromagnetic interactions in 3, in spite of μ-1,1 azide bridged rungs may be due to the crossover near the bridge angle. The ferromagnetic interactions in 1 and 2 are supported by DFT calculations. © The Royal Society of Chemistry.
MTHFR C677T polymorphism is associated with hyperlipidemia in women with polycystic ovary syndrome
(2012) Madhu Jain; Priyanka Pandey; Narendra Tiwary; Shuchi Jain
Context: Women with polycystic ovary syndrome (PCOS) are prone for coronary artery disease (CAD), and hyperhomocysteinemia is an independent risk factor for CAD. MTHFR deficiency is the most common cause of hyperhomocysteinemia, thereby provoking a possible association between PCOS and MTHFR C677T polymorphism. AIMS: The aim of this study was to investigate an association of MTHFR C677T polymorphism with PCOS. Settings and Design: 92 women with PCOS (Rotterdam criteria) and 95 age-matched controls were compared with respect to MTHFR C677T polymorphism. The 2 genotypes (CC and CT) obtained were compared with clinical and laboratory parameters in women with PCOS. Materials and Methods: In a case-control study, clinical, biochemical, hormonal and genetic analysis (PCR-RFLP of peripheral leucocytes) was carried out on all women with PCOS as well as controls. Statistical Analysis: Student "t" test for quantitative and Chi-square test for nominal variables was used. For estimation of risk, odds ratio and 95% confidence interval were calculated. Results: The odds ratio of bearing a heterozygous genotype (CT) was 1.32 in women with PCOS as compared to controls (P = 0.48). No homozygous mutation (TT) was found in the study population. Serum cholesterol was more in heterozygous (CT) genotype (215.48 25.56 mg/dl) as compared to normal (CC) genotype (203.29 16.35 mg/dl) in women with PCOS ( P = 0.01). Similarly, serum triglyceride was more in heterozygous (CT) genotype (95.86 37.34 mg/dl) as compared to normal (CC) genotype (82.36 20.88 mg/dl) in women with PCOS ( P = 0.04). Conclusions: Although not statistically significant, there is a slightly higher prevalence of heterozygous (CT) genotype in women with PCOS. MTHFR C677T polymorphism when present may confer an increased susceptibility to develop hyperlipidemia in women with PCOS. More prospective studies are needed to confirm whether this hyperlipidemia due to MTHFR C677T polymorphism clinically manifests into CAD in long term in women with PCOS.
Novel Supramolecular Assemblies of Co(III) & Cu(II) with Diethylenetriamine and Azide: Synthesis, Structure, Spectroscopic and Magnetic Studies
(Wiley-Blackwell, 2018) Priyanka Pandey; Goulven Cosquer; Masahiro Yamashita; Sailaja S. Sunkari
In exploring the role of increased ligand basicity as well as denticity on moving from ethylenediamine (en) or 1,3-diaminopropane (1,3-dap), on supramolecular solid formation, complexation involving diethylenetriamine (det) and azide with CoII and CuII afforded three new complexes [Co(det)2](N3)3 (1), [Co(det)(N3)3] (2) and [Cu2(det)2(N3)5(H2O)2Na] (3) under self assembling conditions and are characterized structurally and magnetically. Formation of 1 and 2 is sensitive to temperature of crystallization, while such a temperature influence could not be observed for CuII system due to lack of crystals at low temperatures. As expected with a tridentate ligand, strong chelation stabilizes 1 at LT and is a thermodynamically stable product, while isolation of 2 at RT conditions is under kinetic control. Decomposition of 2 at high temperatures (above 70 °C) as revealed through temperature dependent UV-vis spectral studies also confirms its instability. Presence of three donor sites of det, leads to interesting structural pattern in Jahn-Teller active CuII, resulting in cationic chains of [Cu(det)(N3)]n + bridged by μ-1,3 azido bridges to the neighboring [Na(H2O)2(N3)2]− units, thus leading to a 2-D assembly of alternating layers of cationic and anionic chains in the unit cell of 3. Magnetic studies of 3 reveals anti-ferromagnetic interaction between the copper ions (J=– 0.479 cm–1), in conformity with the bridge angle dependent magnetic behavior in azido bridged copper complexes. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Phase diagram and thermal properties of organic analogues of nonmetal-nonmetal systems
(Springer Netherlands, 2016) R.N. Rai; Priyanka Pandey; U.S. Rai
The solid-liquid phase equilibrium data on three binary organic systems, namely 4-hydroxy benzaldehyde (HB)-2-methyl-4-nitro aniline (MNA), 3-hydroxy-4-methoxybenzaldehyde (HMB)-2-chloro-4-nitroaniline (CNA) and 4-aminobenzophenone (ABP)-m-nitrobenzoic acid (NBA), determined by the thaw-melt method, show the formation of a eutectic at 0.60 mol fraction of HB, 0.48 mol fraction of HMB and 0.51 mol fraction of ABP. These systems are organic analogue of nonmetal-nonmetal system. The values of enthalpy of fusion of eutectics and parent components were determined by the DSC method. Using the heat of fusion values, entropy of fusion, heat of mixing, excess thermodynamic functions and Jackson's roughness parameter were calculated. The negative values of enthalpy of mixing in each case suggest the associative interaction between the molecules of the eutectic melt. The positive value of excess free energy for HB-MNA system indicates the associative interaction between like molecules, while the negative values of excess free energy for HMB-CNA and ABP-NBA indicate the associative interaction between unlike molecules. © 2015 Akadémiai Kiadó, Budapest, Hungary.
Solid-liquid equilibrium, thermal, and physicochemical studies on salicylamide-4-nitrophenol and 2-cyanoacetamide-4-aminoacetophenone organic eutectic systems
(2013) Manjeet Singh; Priyanka Pandey; R.N. Rai; U.S. Rai
The solid-liquid phase equilibrium data of two binary organic systems, namely, salicylamide-4-nitrophenol and 2-cyanoacetamide-4-aminoacetophenone show the formation of a eutectic in each case. The values of enthalpy of fusion of pure components and binary eutectics have been determined using differential scanning calorimeter (Mettler DSC-4000 system). The thermal properties of the materials, such as, heat of mixing, entropy of fusion, roughness parameter, interfacial energy, and excess thermodynamic functions were computed using the enthalpy of fusion values. The microstructures of eutectics were developed using unidirectional thermal gradient, and regions of interest for microstructures were photographed. © 2012 Akadémiai Kiadó, Budapest, Hungary.
Synthesis and studies on structural, optical and nonlinear optical properties of novel organic inter-molecular compounds: 4-chloro-3-nitroaniline−3-hydroxy benzaldehyde and urea−4-dimethylaminopyridine
(Elsevier B.V., 2018) Priyanka Pandey; R.N. Rai
Two novel organic inter-molecular compounds (IMCs), (3-(4-chloro-3-nitrophenylimino) methyl) phenol) (CNMP) and urea ̶ 4-dimethylaminopyridine complex (UDMAP), have been synthesized by solid state reaction. These two IMCs were identified by phase diagram study of CNA−HB and U−DMAP systems. The single crystals of newly obtained IMCs were grown by slow solvent evaporation technique at room temperature. Both the IMCs were further studied for their thermal, spectral, single crystal XRD for their atomic packing in molecule, crystallinity, optical and nonlinear optical behaviour. In both the cases, melting point of inter-molecular compounds was found to be higher than that of their parent components, CNMP was found to be thermally stable up to 158 °C while UDMAP was stable up to 144 °C, which indicate their extra stability than their parents. The single crystal XRD studies confirmed that CNMP has crystallized in orthorhombic unit cell with non-centrosymmetric space group P212121 while UDMAP has crystallized in monoclinic unit cell with centrosymmetric space group C2/c. The absorption spectrum of CNMP was found to be in between the absorption of parents, while broadening of peak and red shift was observed in UDMAP as compared to the parents. Second order nonlinear optical property of CNMP and UDMAP was studied using Kurtz Perry powder technique and intense green light emission was observed with CNMP on excitation with 1064 nm of Nd:YAG laser while no emission was observed with UDMAP. © 2018 Elsevier B.V.
Synthesis, spectroscopic, crystal structure, thermal and optical studies of a novel proton transfer complex: 2-Methyl-8-hydroxyquinoliniumpicrate
(Elsevier B.V., 2019) Umesh Neupane; Manjeet Singh; Priyanka Pandey; R.N. Rai
A novel proton transfer complex, 2-methyl-8-hydroxyquinoliniumpicrate (MHQP), has been synthesized in methanol solvent using 2-methyl-8-hydroxyquinoline (MHQ) as proton acceptor and picric acid (PA) as proton donor in 1:1 M ratio, and its single crystal growth, thermal, spectroscopic, optical properties along with atomic packing of crystal have been studied. The DSC and TGA infer the thermal behavior of complex and FT-IR and proton NMR spectroscopic studies infer the charge-transfer interaction via transfer of proton. The powder XRD indicates novelty of the complex and single crystal analysis of MHQP crystal confirms the ionic interaction along with H-bonding interactions between MHQ and PA. The association constant and molar extinction coefficients were studied based on higher absorption band in UV–vis. absorption spectrum of the complex. The significant optical transmittance spectrum of grown MHQP crystal is indicative for its possible applications in opto-electronic devices. © 2019 Elsevier B.V.
Template directed synthesis of half condensed Schiff base complexes of Cu(II) and Co(III): Structural and magnetic studies
(Elsevier Ltd, 2019) Priyanka Pandey; Abhineet Verma; Kateryna Bretosh; Jean-Pascal Sutter; Sailaja S. Sunkari
By the proper choice of the substituents on the aldehyde and amine groups, the nature of the Schiff base formed (fully condensed or half condensed) can be tuned by the templating effect of the metal ion. The condensation of 5-chlorosalicylaldehyde with ethylene diamine in the presence of Co(II) or Cu(II) cations and azido anions has afforded two new half condensed Schiff base metal complexes [CuL(µ1,1-N3)]2 (1) and [CoL2]N3·H2O (2), where L = (E)-2-((2-aminoethyl)methyl)-4-chlorophenol, that were structurally characterized by X-ray analysis, IR and UV spectra. Complex 1 is an asymmetric µ-1,1 azide bridged dimer displaying weak antiferromagnetic interactions (J = −2.93 ± 0.03 cm−1), in agreement with the axial-equatorial N3-linkage between the two copper centers. Complex 2 is a monomer of a low spin Co(III) ion. © 2019
Tetranuclear Copper(II) Complexes with Simultaneous Phenoxo and Azido Bridges - Synthesis, Structural and Magnetic Studies
(Wiley-Blackwell, 2018) Priyanka Pandey; Nidhi Dwivedi; Goulven Cosquer; Masahiro Yamashita; Sailaja Sunkari
Tetradentate Schiff base ligands with multiple coordinaton sites are one of the best choice for the construction of multinuclear metal complexes. Coupled with auxiliary ligands under self assembling conditions, the scope for generating new structures increases manifold due to various structure directing factors in operation. Though, phenoxo or azido bridged copper(II) systems have been investigated thoroughly from structural and magnetic aspects, the literature is scanty with combined phenoxo and azido bridged systems. In a study on the effect of synthetic conditions on the structures generated and associated magnetic properties, two new tetranuclear copper complexes, viz., [Cu4(L1)2(μ-N3)2(N3)2] (1) and [Cu4(L2)2(μ-N3)2(N3)2] (2) where L1=N,N′-Bis(salicylidene)diaminopropane (salpn) and L2=N,N′-Bis(salicylidene)diaminobenzene (salophen) with both phenoxo and azido bridges are reported. The tetranuclear cluster is made of two inversion related dimers built of phenoxo bridged Cu1 and Cu2 with distorted square planar and square pyramidal geometries respectively. These dimers are bridged by symmetrically bridging azides leading to a tetranuclear core. The contrasting magnetic behavior inspite of same coordination environments, antiferromagnetic in 1 (J1=−2.17 cm−1; J2=−28.15 cm−1) and ferromagnetic in 2 (J1=−74.7 cm−1; J2=146.5 cm−1), is understood in terms of lower bridge angles and closer separation between copper centers in 2 than in 1. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim