Browsing by Author "Priyatama Behera"

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Fabrication of bioactive transparent glass ceramics 55SiO2–25Na2O-(15-x)CaO–5P2O5- xZrO2 (0≤x≤6): Physical, structural and in vitro cell viability insights for biomedical applications
(Elsevier Ltd, 2024) Shweta; Rajat Kumar Mishra; Bijay Laxmi Pradhan; Shama Parveen; Priyatama Behera; Sarvesh Kumar Avinashi; Savita Kumari; Zaireen Fatima; Prince Sen; Saurabh Kumar; Monalisa Mishra; Monisha Banerjee; Krishna Kishor Dey; Manasi Ghosh; Chandkiram Gautam
Zirconia (ZrO2) reinforced transparent glass ceramics (TGCs) are excellent materials for enhanced cell viability and biocompatibility for biomedical applications. Herein, ZrO2 doped SiO2–Na2O–CaO–P2O5 base compositions derived from traditional melt-quench technique. The impacts of ZrO2 on the physical, structural, optical, morphological, and biological evaluation were studied. XRD discloses the major phase formation of buchwaldite (CaNaPO4) and disodium calcium silicate (Na2CaSiO4). Density of the TGC samples was calculated and found to be in the range of 2.535–2.910 g/cm3. The optical parameters, and particle size were estimated and analyzed. Herein, ZrO2 plays a significant role as a network modifier and various bond assignments in the glassy network that confirmed by FTIR spectroscopy. Surface morphology and its elemental investigations were also studied using SEM and XPS techniques. Solid state NMR spectrum on 23Na, 29Si, and 31P nucleus were studied for various interactions. Moreover, the cell viability of the fabricated samples on the cancer cells were analyzed and resulted to possess the half maximum inhibitory concentrations (IC50) of the samples were calculated and valued to be 92 to 40 μg/ml respectively. The sample 55SiO2–25Na2O–9CaO–5P2O5–6ZrO2 (BG4) demonstrated a remarkable biological activity for bone regeneration and implants. Further, the cell cytotoxicity was evaluated by performing trypan blue assay, DAPI and DCFH-DA staining on the TGC samples. The data assembled in this research approves the hypothesis that the TGCs represent a feasible material for the biomedical applications for bone and tooth implants. © 2024 Elsevier Ltd and Techna Group S.r.l.
Influence of TiO2as a filler into PMMA-ZrO2composites on structural, mechanical and biological properties for denture fabrication
(Elsevier Ltd, 2025) Akhileshwar Gupta; Savita Kumari; Sarvesh Kumar Avinashi; Rajat Kumar Mishra; Rupesh Kumar; Priyatama Behera; Monalisa Mishra; Rakesh Kumar C. Gautam; Saurabh Kumar; Shama Parveen; Jitendra Rao; Monisha Banerjee; Chandkiram Gautam
Polymethyl-methacrylate (PMMA) is widely being used in dentistry, while it has limited mechanical strength. Herein, PMMA composites were fabricated using heat cure technique within a system [(95-x) PMMA + 5 ZrO2 + x TiO2] (x = 8, 6, 4 and 2 wt%) and thoroughly characterized using density measurements, XRD, FTIR, SEM, EDAX, compression tests, 3-point bending, tribological, and hardness tests respectively. Moreover density, crystallite size, percentage of crystallinity, compressive strength, Young's modulus, flexural strength, flexural modulus, modulus of resilience, modulus of toughness, and brittleness coefficient were determined. Composite contains 5 wt% ZrO2and 2 wt% TiO2referred as ZPT2 exhibited the maximum compressive strength. Additionally, flexural strength and flexural modulus were found to be highest for ZPT2. The friction coefficient and hardness were found to be the lowest for ZPT2. In MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5- diphenyl tetrazolium bromide) assay, MG-63 osteoblast cells were exposed to various concentrations (5–30 μg/ml) of the compounds for 24 h. The results indicated that no significant cytotoxicity was observed for ZPT2. According to the results of the dye exclusion experiment, DAPI (4, 6-diamidino-2-phenylindole), and DCFH-DA (2, 7-dichlorodihydrofluorescein diacetate) staining, ZPT2 did not exhibit cellular damage, genotoxicity and reduced ROS (reactive oxygen species) production at lower concentrations. The combined biological results suggest that oral administration of the ZPT2 sample does not exhibit cytotoxicity and genotoxicity that makes it valuable for dentistry. © 2025 Elsevier Ltd and Techna Group S.r.l. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Preparation of ZrO2–ZnO-Poly-Methyl-Methacrylate Composite with Enhanced Physical and Mechanical Properties for Dentistry
(Springer, 2025) Akhileshwar Gupta; Rashmi Rekha Devi; Savita Kumari; Anshu Raj; Priyatama Behera; Monalisa Mishra; Rakesh Kumar C. Gautam; Shama Parveen; Saurabh Kumar; Jitendra Rao; Monisha Banerjee; Chandkiram Gautam
Poly-methyl-methacrylate (PMMA) is the most widely used denture material for various dental applications. There are certain limitations associated with PMMA, such as its mechanical strength, dimensional stability and biocompatibility. In this study, efforts have been made to overcome these shortcomings by using ZrO2 and ZnO as fillers. The main motive of the study is to fabricate ZrO2 and ZnO reinforced PMMA composites with superior physical and mechanical properties. Herein, numerous composites were synthesized within a system [(95−x) PMMA + 5 ZrO2 + x ZnO)] (x = 2, 4, 6 & 8% wt.) using a conventional heat cure method. A comprehensive evaluation of the PMMA-ZrO2–ZnO (PZZ) composites was carried out through both theoretical and experimental density measurements, along with a range of characterization techniques including XRD, SEM, compression and three-point bending tests, water sorption analysis, tribological testing, hardness assessment, and biological assays such as MTT, DAPI, DCFH-DA, and trypan blue staining. As the ZnO content increased, there was a corresponding rise in the density, crystallinity percentage, Young’s modulus, and flexural modulus of the composites. However, an increase in ZnO content led to a reduction in compressive strength, fracture toughness, and flexural strength of the PZZ composites. Biological evaluation indicated that cell viability was highest in composites with lower ZnO concentrations. Overall, this study provides comprehensive insights into the physical, mechanical, and biological properties of the synthesized PMMA-ZrO2–ZnO composites. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Synergetic impact of MgO on PMMA-ZrO2 hybrid composites: Evaluation of structural, morphological and improved mechanical behavior for dental applications
(Elsevier Inc., 2024) Savita Kumari; Anuj Verma; Rajat Kumar Mishra; Sarvesh Kumar Avinashi; Shweta; Shweta Singh; Priyatama Behera; Jitendra Rao; Rakesh Kumar Gautam; Bijay Laxmi Pradhan; Krishna Kishor Dey; Manasi Ghosh; Monalisa Mishra; Chandkiram Gautam
This work aims to demonstrate the effect of ZrO2 and MgO inclusion into the Poly(methyl methacrylate) (PMMA). To fabricate novel hybrid composites via heat cure method, various composites (PZM2, PZM4 and PZM6) were synthesized in the system [(95-x) PMMA + 5 ZrO2 + x MgO] (x = 2, 4, and 6) respectively. Density of the prepared composites were determined and varying between 1.035–1.152 g/cm3. X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) followed by EDAX and mechanical testing were performed to evaluate the fabricated composite properties. Moreover, to explore the structure of the fabricated composites the 13 C CP-MAS SSNMR and 1 H-13 C Phase-Modulated Lee Goldberg (PMLG) HETCOR Spectrum were recorded which clarify chemical shifting and motional dynamics of the composites. Mechanical tests were performed by UTM and the obtained parameters such as compressive strength, Young's modulus, fracture toughness, brittleness coefficient, flexural strength and flexural modulus are found to be in the range of 91–100 MPa, 0.48–0.51 GPa, 9.122–9.705 MPa.m1/2, 0.66–0.815, 51.03–42.78 MPa and 499–663 MPa respectively. Some more mechanical parameters such as proportional limit, elastic limit, failure strength, modulus of resilience and modulus of toughness were also calculated. Furthermore, tribological properties were also determined and the coefficient of friction (COF) was decreased by 17.4 % and 38 % for composite PZM6 at 20 N and 40 N as compared to the composite PZM2 and the lowest wear volume of 1.55 mm3 was observed for PZM2, whereas the maximum volume loss of 5.64 mm3 is observed for composite PZM6. To check out the biocompatibility, cytotoxicity and genotoxicity of the fabricated composites the Trypan-blue assay was also performed for PZM2 and PZM6 composites. Dissection on the gut of larvae was also performed on the both composites followed by DAPI and DCFH-DA staining. Therefore, these synthesized samples can be used for the fabrication of denture materials. © 2024 Elsevier Inc.