Browsing by Author "Punuri Jayasekhar Babu"

Now showing 1 - 2 of 2

Cyanobacterial availability for CRISPR-based genome editing: Current and future challenges
(Elsevier, 2022) Sandeep Kumar Singh; Ajay Kumar; Avinash Chandra Rai; Mukesh Kumar Yadav; Punuri Jayasekhar Babu; Zothanpuia; Liansangmawii Chhakchhuak; Prashant Kumar Singh; Garima Singh; Naveen Chandra Joshi; Avinash Singh; Kaushalendra; Rosie Lalmuanpuii; Esther Lalnunmawii; Bendangchuchang Longchar
Cyanobacterial genetic manipulations are optimistic for producing feedstocks, fuels, valuable chemicals, and a basic understanding of stress-induced responses. Regrettably, the newly available genome manipulation tools for cyanobacteria are far from other organisms despite their significant contributions to humanity. This chapter primarily focused on genome engineering efforts available to date for synthetic biology applications and the recent advances in investigations in the development of genome editing in cyanobacteria. Moreover, in recent years, clustered regularly interspaced short palindromic repeats (CRISPR) dependent approaches rapidly gained engineering popularity in all life domains. Such techniques permit markerless genome editing, simultaneous manipulation of multiple genes, and transcriptional regulation of genes. However, the cyanobacterial genome manipulations by employing the CRISPR tool are still in infancy and limited to very few reports for its synthetic applications, even though the CRISPR drastically shortened the mutant selection time and the segregation advantages. In this chapter, we highlighted the studies that have implemented CRISPR-based tools for cyanobacteria’s metabolic engineering. © 2022 Elsevier Inc. All rights reserved.
Cyanobacterial photosynthetic reaction center in wobbly light: Modulation of light energy by orange carotenoid proteins (OCPs)
(Elsevier, 2022) Rahul Prasad Singh; Sandeep Kumar Singh; Ajay Kumar; Arpan Modi; Avinash Chandra Rai; Sandip Ghuge; Anil Kumar; Mukesh Kumar Yadav; Punuri Jayasekhar Babu; Prashant Kumar Singh; Garima Singh; Kaushalendra; Naveen Chandra Joshi; Avinash Singh; Wenjing Wang
High irradiance and fluctuating light boons substantial risk to photosynthetic life forms by summoning responsive oxygen species (ROS). To bear the high irradiance level, plants, algae, and cyanobacteria have developed mechanisms to diminution the energy hitting at reaction centers to protect it from high irradiance by a photo-defensive system. In cyanobacteria, these photoprotection systems reduce the light energy arriving at the reaction centers by reducing photosynthesis and enhancing thermal energy dissipation at the level of the phycobilisome (PB), the extra-membranous light-harvesting antenna. Fluorescence recovery proteins (FRPs) and orange carotenoid proteins (OCPs) alluded to as essential elements for this mechanism by nonphotochemical quenching (NPQ). Initially, cyanobacteria were considered not to fit for performing NPQ, and the last shreds of evidence advocated NPQ as a crucial and primary photoprotective tool. OCP comprises two domains, (1) N-terminal domain (NTD) and (2) C-terminal domain (CTD), with a solitary carotenoid as a chromophore traversing evenly in the two areas. Blue-green or high irradiance light actuates the transformation of OCP from a dormant orange state (OCPO) to a dynamic red state (OCPR). Dynamic OCP (OCPR) ties to the center of the light-harvesting antenna complex, phycobilisomes (PBs), extinguishes fluorescence, and aids dispersal abundance energy’s nonradiative pathway. OCP-intervened photoprotection mechanism effectively managed by FRP by accelerating the OCP transformation of active OCP (OCPR) to the resting state (OCPO) under light-limiting conditions. However, numerous inquiries concerning the working of FRP have stayed dubious. This chapter summarizes the current information and comprehension of the FRP and OCP in cyanobacterial photoprotection and the possibilities of exploiting these systems for plant resilience to high irradiance. © 2022 Elsevier Inc. All rights reserved.