Browsing by Author "R. Mishra"

Now showing 1 - 10 of 10

A zinc(II) directed triple-stranded helicate incorporating a nine membered metallamacrocycle: Supramolecular cylinders mimicking P1 nuclease
(2012) Rakesh K. Mishra; K.K. Upadhyay; Sachin Shukla; R. Mishra
We report herein the first small molecule based self-assembled fluorescent trinuclear Zn(II) supramolecular cylindrical motif as a structural and functional model of the active site of P1 nuclease. The DNA binding of the same was investigated through UV-Vis, fluorescence and circular dichroism studies. © 2012 Royal Society of Chemistry.
Deciphering the link between Diabetes mellitus and SARS-CoV-2 infection through differential targeting of microRNAs in the human pancreas
(Springer Science and Business Media Deutschland GmbH, 2022) Bhavya; E. Pathak; R. Mishra
Purpose: Coronavirus Disease 2019 (COVID-19) severity and Diabetes mellitus affect each other bidirectionally. However, the cause of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection on the incidence of diabetes is unclear. In the SARS-CoV-2-infected cells, host microRNAs (miRNAs) may target the native gene transcripts as well as the viral genomic and subgenomic RNAs. Here, we investigated the role of miRNAs in linking Diabetes to SARS-CoV-2 infection in the human pancreas. Methods : Differential gene expression and disease enrichment analyses were performed on an RNA-Seq dataset of human embryonic stem cell-derived (hESC) mock-infected and SARS-CoV-2-infected pancreatic organoids to obtain the dysregulated Diabetes-associated genes. The miRNA target prediction for the Diabetes-associated gene transcripts and the SARS-CoV-2 RNAs has been made to determine the common miRNAs targeting them. Minimum Free Energy (MFE) analysis was done to identify the miRNAs, preferably targeting SARS-CoV-2 RNAs over the Diabetes-associated gene transcripts. Results : The gene expression and disease enrichment analyses of the RNA-Seq data have revealed five biomarker genes, i.e., CP, SOCS3, AGT, PSMB8 and CFB that are associated with Diabetes and get significantly upregulated in the pancreas following SARS-CoV-2-infection. Four miRNAs, i.e., hsa-miR-298, hsa-miR-3925-5p, hsa-miR-4691-3p and hsa-miR-5196-5p, showed preferential targeting of the SARS-CoV-2 genome over the cell’s Diabetes-associated messenger RNAs (mRNAs) in the human pancreas. Conclusion: Our study proposes that the differential targeting of the Diabetes-associated host genes by the miRNAs may lead to diabetic complications or new-onset Diabetes that can worsen the condition of COVID-19 patients. © 2021, Italian Society of Endocrinology (SIE).
Myths, facts and scope of spinal cord tolerance dose revision in Intensity modulated SIB treatment of locally advanced head and neck cancer: A dosimetrical and radiobiological demonstration
(Elsevier Masson s.r.l., 2021) G. Patel; A. Mandal; S. Choudhary; R. Mishra; U. Shahi; H. Mishra
Purpose: To explore the possibility of revising the spinal cord tolerance dose in Simultaneously Integrated Boost (SIB) intensity modulated treatment plan of locally advanced head and neck (H&N) cancer and assessment of achieved planning gain due to the revision. In SIB regimen, the Organ at Risk (OARs) tolerance dose is equally distributed throughout the treatment. Clinicians have usually considered the spinal cord tolerance to be the same as in conventional technique. However, in SIB fractionation regimen with intensity modulation treatment, the spinal cord may receive a physical dose of 45 Gy, with much lesser dose per fraction than 2 Gy per fraction. So when the dose of spinal cord is distributed throughout the treatment, the tolerance dose limit of physical dose can be considered higher than the usual conventional dose limits. In this study, an attempt has been made to explore the possibilities of dose escalation and treatment planning benefits while exploiting this “Window of Opportunity (WoO)” of increase in spinal cord and Planning Risk Volume (PRV) spinal cord tolerance dose. Material and methods: A total of 12 patients CT data set along with approved structure set of H&N cancer used for treatment planning in. Three independent SIB VMAT plans named as SPC, SPR and SPDE were generated for the 12 patients. First plan (SPC) was generated by considering standard spinal cord tissue constraint of maximum dose of 45 Gy and PRV spinal cord maximum dose 50 Gy as per QUANTEC summary and second plan (SPR) was generated considering spinal cord tissue constraint of maximum dose 52.50 Gy and PRV spinal cord maximum dose 56.35 Gy while optimization and dose calculation. The objectives for rest of the Organ at Risk (OAR) were kept same in both the plans during optimization and dose calculation. The SPC plan was copied for creation of third plan (SPDE) in which dose was escalated by increasing dose per fraction for target volumes such that dose to spinal cord reached a maximum dose of 52.50 Gy and PRV spinal cord maximum dose of 56.35 Gy. In this plan there have been changes to only dose per fraction, however dose optimization and dose calculation have not been performed. Radiobiological parameters TCP and NTCP were also calculated by using indigenously developed software. Results: Considering the increase of spinal cord tolerance dose as “window of opportunity”, a sufficient escalation in physical dose, Biological Effective Dose (BED) and Tumor Control Probability (TCP) was observed for all target volumes with acceptable level of NTCP values. Conclusion: Sufficient dose escalation and increased in TCP for target volumes or effective planning benefits can be achieved by revising the spinal cord tolerance dose in intensity modulated SIB treatment of locally advanced H&N cancers. © 2020 Société française de radiothérapie oncologique (SFRO)
Performance on a mirror-drawing task by non-right-handers
(2000) Braj Bhushan; C.B. Dwivedi; R. Mishra; Manas K. Mandal
Left-, mixed-, and right-handed (each n = 10, N= 30) adolescent boys who were classified on the basis of a hand preference inventory performed a mirror-drawing task with a bilateral transfer of a skill paradigm. Participants' hand preference and the magnitude of bilateral transfer of skill were assessed in terms of errors committed and time taken to complete the mirror-drawing task. Mixed-handed participants exhibited significantly less habit interference for mirror drawing, and they performed the task significantly faster than the left-handers did; the group difference was not significant for the frequency of errors committed. These groups did not differ in terms of the magnitude of bilateral transfer of skill; the trend, however, showed that the transfer of skill was minimum in mixed-handers. These findings extend the theory that mixed-handed participants' inability to transfer motor skill from one hand to the other could be attributable to their lack of a clear pattern of lateralization. Their ability to perform well either at initial or later trials may be a function of less interference from their normal motor habits.
Serum adenosine deaminase levels as an index of tumor growth in head and neck malignancy
(2000) R. Mishra; M.K. Agarwal; J.P.N. Chansuria
This study was conducted on 40 biopsy proved patients of head and neck cancers. 85% of patients presented with squamous cell carcinoma in various grades of differentiation. When seruma denosine deaminase activity was compared between controls and cases, significant increase was found in the activities (control 51.54 ± 12.09 IU/L and cases 106.87 ± 29.75 IU/L). The duration of illness didn't reflect any statistical significance with the adenosine deminase activity. It was 97.50 ± 62.93 IU/L in case where duration of illness was 98 ± 30.98 IU/L in patient with more than one year of disease. The lymph node showed stronger correlation with adenosine deaminase activity. Its level was 83.41± 1.41 IU/L in patients with N3. The rise in serum adenosine deaminase activity was found to be directly related to the stage I disease. It was 57.80 ± 4.60 IU/L L in patient with stage I disease while in patients with stage IV had 135.87 ± 18.39 IU/L of activity. According to histological grading, highest level was found in patients having squamous cell carcinoma(113.41 ± 32.31 IU/L). The activity of adenosine deaminase decreases with radiotherapy and after surgery. This may help in assessing the decrease in tumour mass and improvement in patient's clinical condition.
Sunthi (Dry Zingiber Officinale) as a Prophylactic Agent Against SARS-CoV-2 Transmission and COVID-19 Symptomatology: Evidence From a Non-randomised Single-Arm Study
(Elsevier GmbH, 2023) S.K. Dubey; S.K. Mishra; V. Singh; Y.B. Tripathi; R.N. Chaurasia; P.S. Byadgi; H. Jani; R. Mishra; N. Joshi; T.B. Singh; A. Kumar; A. Yadav; A. Jaiswal
Introduction: Ancient Indian medical system Ayurveda cites Sunthi (an ayurvedic preparation of dry Zingiber officinale) as ‘Vishvabhesaja’—medicine of highest relevance for the whole world—upholding its special status in managing pandemics. The present study assessed the prophylactic response of Sunthi in reducing SARS-CoV-2 transmission, moderating COVID-19 symptomatology, and improving individuals’ quality of life. The COVID-19-relevant phytochemicals in Z.officinale were analysed, and the prominent predictive factors for quality of life were identified. Methods: This study used multicentre, non-randomised, open-label, single-arm, pre-post design. The sample (n = 824) comprised household members of hospitalised coronavirus-infected patients (n = 770) and the frontline health care workers (n = 54) operating in COVID-19 hospitals. Participants consumed Sunthi powder four times daily, twice via oral (2 g) and twice via nasal (0.5 g) routes, for 15 days. They were followed up after 15, 30, and 90 days. For phytochemical analysis, liquid chromatography coupled with mass spectrometry, and for data analysis, McNemar's test, repeated measures t-test, and multiple linear regression were performed. Results: Z.officinale comprises phytoconstituents with anti-SARS-CoV-2 properties, has no severe effects, alleviates coronavirus-compatible symptoms, and improves the quality of life. Conclusions: Dry Z.officinale can be a safe, effective, readily deployable, and affordable treatment for preventing coronavirus infection and managing COVID-19 symptomatology. Incorporating it into the COVID-19 prevention and management protocol can be of relevance for public health systems globally. However, this is a preliminary observation that may be informative for future research. No causal inference pertaining to the observed association could be drawn, and this result shall be interpreted with caution. © 2023 Elsevier GmbH
Synthesis, crystal structure and nuclease activity of a Cu(II) complex having two different co-ordination geometries in the same unit cell
(Elsevier B.V., 2013) Virendra Kumar; Rakesh K. Mishra; Sachin Shukla; R. Mishra; Monali Singh; Ida Tiwari; Kamlesh Thapliyal; K.K. Upadhyay
A new Cu(II) complex of a Schiff base ligand having N2O donor set has been synthesized and fully characterized through its single crystal X-ray studies. Two different co-ordination patterns viz. square planar and square pyramidal for Cu(II) were observed within the same unit cell. The DNA binding of copper(II) complex was investigated through UV-vis, fluorescence as well as cyclic voltammetric studies. The complex exhibited efficient functional mimicking of nuclease activity over the plasmid pBR322 DNA. © 2013 Elsevier B.V. All rights reserved.
Synthesis, structural investigation, DNA and protein binding study of some 3d-metal complexes with N′-(phenyl-pyridin-2-yl-methylene)-thiophene-2- carboxylic acid hydrazide
(Elsevier B.V., 2014) Monika Mishra; Karishma Tiwari; Sachin Shukla; R. Mishra; Vinod P. Singh
The ligand, N′-(phenyl-pyridin-2-yl-methylene)-thiophene-2-carboxylic acid hydrazide (Hpmtc) derived from thiophene-2-carboxylic acid hydrazide and 2-benzoyl pyridine, and its metal complexes with Co(II), Ni(II), Cu(II) and Zn(II) have been synthesized. These compounds are characterized by elemental analyses, magnetic susceptibility measurements, IR, NMR and UV-Vis spectral studies. The molecular structures of Hpmtc and its Co(II) (1), Ni(II) (2), Cu(II) (3) and Zn(II) (4) complexes are finally determined by X-ray crystallography. Various spectral and single-crystal X-ray diffraction studies suggest that Hpmtc coordinates with metal ions as a monobasic tridentate ligand forming mononuclear distorted octahedral complexes of the type [M(pmtc) 2]. The molecular structures of the complexes are stabilized by CH-N, CH-O intermolecular H-bonding, and CH-π and π-π interactions. The DNA binding experiment of the complexes 1, 3 and 4 by UV-Vis absorption, and EB-DNA displacement by fluorescence spectroscopy, reveal an intercalative mode of binding between CT-DNA (calf-thymus DNA) and the metal complexes. These complexes exhibit a moderate ability to cleave pBR322 plasmid DNA. A comparative bovine serum albumin (BSA) protein binding activity of the complexes 1, 3 and 4 has also been determined by UV-Vis absorption and fluorescence spectroscopy. The DNA binding and protein binding studies suggest that the complex 3 exhibits more effective binding activity (Kb = 5.54 × 105 and Kq = 1.26 × 106 M-1, respectively) than complexes 1 and 4. However, the complex 1 shows better hydrolytic DNA cleavage activity compared to 3 and 4 complexes. © 2014 Elsevier B.V. All rights reserved.
Treatment seeking in reproductive age women with RTIs/STIs: A community based cross-sectional study
(2011) M.A. Hussain; R. Mishra; S. Kansal; C.P. Mishra; S.K. Jha; H. Hasan
The socio-cultural and economic contexts in developing countries influence the epidemiology or STls and helps in making them an important public health priority. This study was carried out to explore the health care seeking pathway of the women suffering from RTls/STls. influence or major socio-demographic variables on treatment seeking pattern. This cross sectional study was carried out comprising or eight hundred reproductive age (15-49 years) women selected following a multistage sampling procedure. The information pertaining to health seeking behaviour were collected from them using a pre-designed pretested interview schedule. Quantum of RTls/STls in the study group was estimated and symptomatics were asked specially about time lag between appearance of symptoms and seeking acre; reason for not seeking care; outcome of treatment they have taken if any. Out of total 359 subjects with symptoms of RTls/STls only about one-third (37.3%) had sought treatment. Only a few (4.5%) had sought treatment within 1 month of appearance of symptoms. Considering it to be physiological about half of the subjects (45.3%) did not seek treatment. The treatment seeking pattern was highly associated with the level of education (p=0.000). Reluctance in seeking treatment, delay in its initiation, prelerencc for unqualified practitioners in first consultation and significant association between education and treatment seeking pattern emphasize BCC and service provision for control and prevention of RTls/STls.
Willems I and Willems II Methods for Dental Age Assessment in Children and Adolescents aged 3–16 years in the Varanasi Region: Applicability and Comparability
(Indian Academy of Forensic Medicine, 2024) R. Mishra; R. Mourya; V.K. Srivastava; S.K. Pandey; N.P.G. Kumar
In order to create new, simplified tables (Willems I method) for males and females in the Belgian Caucasian population, Willems G et al. (2001) reexamined Demirjian's technique. With these tables, dental age can be represented directly in years without the need for additional conversion tables. Using the prior data and a new study group, Willems G et al. (2010) created a common table for males and females to overcome cases of unknown gender and created a non-gender-specific method (Willems II method) that was not gender-specific. This study aims to evaluate the Willems I and Willems II methods for age estimation in a sample of the Varanasi region population. In this cross-sectional study, 432 samples (237 boys and 195 girls of age range 3–16 years) from the population of Varanasi region were evaluated to validate the Willems I and Willems II age estimation methods. A paired t-test was applied to determine the statistical significance between estimated dental age and chronological age. The Willems I method underestimated the dental age in boys by-0.27 ± 0.80 years and in girls by-0.60 ± 0.95 years. The Willems II method also underestimated the age by-0.57 ± 0.86 years in boys and-0.38 ± 0.93 years in girls. Pearson correlation revealed a strong positive association in both methods. The Willems I and Willems II methods are not exactly applicable, but the Willems I method is more relatable to the actual age of the boys sample and the Willems II method for the girls sample. © 2024, Indian Academy of Forensic Medicine. All rights reserved.