Browsing by Author "R. Sinha"

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Binding of Ru(II) polypyridyl complexes with DNA
(2001) L. Mishra; A.K. Yadaw; R. Sinha; A.K. Singh
In the present review salient features of ruthenium nucleic acid binding and factors governing the binding affinity with different forms of DNA are presented. Attempts are also made to explore the mode of binding through spectroscopic techniques and correlation of spectroscopic parameters in the presence and absence of DNA.
Conjoined twins with a single heart: A rare case report
(2011) S. Pandey; S. Mendiratta; S. Pandey; R. Sinha; L.K. Pandey
A case of abdomino-thoracopagus twins with a single heart is described. The male twins were delivered in the 15th week of gestation following the parents' request to terminate the pregnancy. This case is of particular interest because of the rarity of the abdominothoracopagus twins with a single heart, in the literature.
Construction of Ru(II) polypyridyl based macrocycles: Synthesis, characterization, electrochemical, Li+ binding, antitumour and anti-HIV properties
(Freund Publishing House Ltd, 2001) L. Mishra; R. Sinha; P.C. Pandey
Some ruthenium (II) polypyridyl complexes with a bis-chalcone (obtained by the condensation of 3-methyl-thiophene-2-carboxaldehyde and 4-acetyl pyridine) have been synthesized and characterized spectroscopically (IR, NMR, UV/Vis), conductimetric, elemental analysis and FAB mass data. Their luminescent, redox and Li+ binding properties have been studied. The anti-HIV and antitumour activities have also been reported.
High fat diet induces obesity in British Angora Rabbit: A model for experimental obesity
(2009) S. Dhungel; R. Sinha; M. Sinha; B.H. Paudel; N. Bhattacharya; M.B. Mandal
A reliable and cost-effective animal model for human obesity with its manifested disorders is yet to be established in the context of increased morbidity and mortality due to obesity and its related problems. Therefore, an attempt was made to produce obesity in locally available British Angora Rabbits (BAR) and examine the effect on metabolic and cardiovascular parameters. Adult male BARs weighing nearly 2 kg were randomly divided into two groups, one of the groups was fed with high fat diet (HFD) ad libitum for 10 weeks and the control group received standard normal rabbit chow for same period. Body weight, skinfold thickness, serum cholesterol, serum glucose and resting heart rate were measured before and after the dietary regimens. After 10 weeks, HFD group of rabbits demonstrated significant (P<0.05) increase in body weight (+24%) and skinfold thickness (+37%). The gain in body weight was positively correlated to skinfold thickness (r=0.61). Serum cholesterol, serum glucose and resting heart rate were also increased by 46%, 52% and 15%, respectively. Whereas no such increases in any of these parameters were observed in control group of rabbits. Our results suggest that obesity can be produced in BARs by feeding HFD. The obesity manifests with cardiovascular and metabolic changes. It is proposed that this may serve as a valid and reliable model of experimental obesity.
HPV 16 DNA sequences in different grades of cervical lesions
(1996) M. Katyayani; B. Chatterjee; R. Sinha; M. Matah; M. Mohan Kumar; G.R.K. Rao
A simple rapid method for isolation of genomic DNA from cervical scrape cells has been standardized. Sufficient amount of good quality genomic DNA was isolated from each cervical scrape specimen. Total 120 cervical scrape specimens from wide spectrum of cervical lesions were analysed for the presence of HPV 16 DNA by Southern hybridization using 32P labelled HPV 16 DNA as probe under stringent conditions. Viral DNA sequences were detected in 78 of the specimens. About 36% (5 out of 14) of normal cervical smears and 59% (46 out of 78) of inflammatory cervical smears had the viral DNA sequences. Except a lone sample, the rest 27 samples of different grades of dysplasia specimens and squamous cell carcinoma samples had viral DNA sequences. Due to development of sensitive Southern hybridization method, the presence of HPV 16 DNA could be detected in high percentage of specimens belonging to the inflammatory cervical specimens.
Mononuclear and binuclear ruthenium(III) polypyridyl complexes containing 2,6-bis(2′-benzimidazyl)-pyridine as co-ligand: Synthesis, spectroscopic properties and redox activity
(2000) L. Mishra; R. Sinha
A series of complexes of the general formula [Ru Hbbip X Cl]+, [Ru Hbbip (X)2]2+ and [(Ru Hbbip X)2 pyz]3+; H2bbip = 2,6-bis-(2′-benzimidazyl) pyridine; pyz = pyrazine and X = 2.2′-bipyridine/1,10-phenanthroline have been synthesized and characterized by their elemental analysis, spectral (IR, 1H NMR, UV-visible and ESR) and redox data. Comparative luminescent behaviour of the complexes in the presence and absence of calf-thymus DNA has also been studied.
Obesity attenuates formalin-induced tonic pain in British Angora rabbits
(2009) R. Sinha; S. Dhungel; M. Sinha; B.H. Paudel; N. Bhattacharya; M.B. Mandal
Obesity is known to alter various physiological parameters including the pain sensitivity. There are conflicting reports on the pain sensitivity in obesity. In this context, the present study was aimed to investigate the tonic pain response in obese rabbit model. To achieve this aim, two groups of adult male British Angora rabbits were used. One of the groups was fed with standard rabbit chow and served as control. The other group was fed high fat diet (HFD) for 10 weeks to produce obesity. The standard formalin test was performed at the start and after 10 weeks of dietary regimen in both the groups. Timed behavioral responses (limping, elevation of paw, licking, biting, grooming etc.) were categorized and quantified with the help of standard pain rating scale. The total average pain rating score decreased significantly from 2.01 ± 0.02 to 1.47 ± 0.08 (P<0.05) in HFD group after 10 weeks of dietary regimen, whereas there was no change in the control group. A significant negative correlation was observed between body weight and pain rating score in HFD group of rabbits (P<0.05, r=-0.62). Results suggest that obesity attenuates the tonic pain responses induced by formalin in British Angora rabbits.
Reply to " Comment on 'Shallow sub-surface stratigraphy of interfluves inferred from vertical electrical soundings in western Ganga plains, India'" by S.K. Lahiri
(2011) G.S. Yadav; A.S. Dasgupta; R. Sinha; T. Lal; K.M. Srivastava; S.K. Singh
[No abstract available]
Shallow sub-surface stratigraphy of interfluves inferred from vertical electric soundings in western Ganga plains, India
(2010) G.S. Yadav; A.S. Dasgupta; R. Sinha; T. Lal; K.M. Srivastava; S.K. Singh
The application of geophysical surveys such as Vertical Electric Sounding (VES) for inferring shallow sub-surface stratigraphy in the vast alluvial tract of India has been under-utilized. This paper is aimed at demonstrating the potential of resistivity methods for mapping alluvial stratigraphy in parts of the Ganga plains where the availability of exposed sections is scarce and bore logs are very limited. Data was generated from 40 vertical electrical resistivity soundings in a stretch of ∼150. km in the interfluve areas between the Ganga and Yamuna rivers and Yamuna and Betwa rivers in the western Ganga plains. Based on the available lithological data from cliff sections along the major and minor rivers draining the study area and a series of 7 drill cores along the transect, the resistivity sounding data was calibrated to interpret the sub-surface stratigraphy. The data shows the vertical extension of the valley fills and interfluve sequences down to ∼100. m. A high-resistivity layer runs quite extensively across the interfluves and below the valley fills, interpreted as a calcrete layer that may mark a major discontinuity. Patches of very low resistivity layers in valley fills as well as interfluves suggest the presence of saline aquifers. This study demonstrates significant sub-surface heterogeneity across the interfluves, which is a manifestation of variable fluvial activity over a period in excess of 100. ka. Lateral continuity of the sandy aquifers is often broken by muddy deposits of the floodplains which form a dominant component of the interfluve sequence. Except for the locations close to major valleys, there are no major sand bodies in the upper ∼100. m of the interfluves which suggests that the modern configuration of the valleys and interfluves in this region is of Late Quaternary antiquity. A few buried sand bodies, however, are comparable to the dimensions of the minor rivers draining the interfluve surfaces. © 2010 Elsevier Ltd and INQUA.
Synthesis, characterization, luminescent and redox properties of some mononuclear and dinuclear ruthenium(II) polypyridyl complexes
(2000) L. Mishra; R. Sinha
A series of mononuclear and dinuclear Ru(II) complexes of the type [RuL(X)2]n+ and [Ru2L′(X)4]n+, L=L1H, n = 1; L=L+ n=2; L′=L3H2, n=2; L′=L4, n =4 and X=2,2′- bipyridyl as well as 1,10-phenanthroline, have been prepared and characterized by IR, 1H-NMR UV-vis spectra, FAB Mass and elemental analysis data. The complexes display metal-ligand charge-transfer (MLCT) transitions in the visible region and show hyperchromic shift upon addition of DNA solution. Emission observed in the range 580-595 nm is quenched upto 7.3-40.5% upon addition of buffered solution of calf thymus DNA. Acetonitrile solution of the complexes show electrochemical oxidation of the ligands.
tris-(Benzimidazol-2-yl-methyl)-amine as a versatile building block in Ru(II) polypyridyl chemistry
(2002) L. Mishra; R. Sinha
tris-(Benzimidazol-2-yl-methyl)-amine, H3ntb, was prepared and used in the synthesis of dinuclear Ru(II) polypyridyl and polynuclear Ru(II)-Co(III) complexes of the type [Ru2(H2ntb) (bpy)4]3+, [Ru2(Hntb)(phen)4]2+, [(Ru2(H2ntb)(bpy)4)2Co(en) 2]9+, and [(Ru2(Hntb)(phen)4)2 Co(en)2]7+ (bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, en = 1,2-diaminoethane). The complexes were characterized by elemental analysis as well as spectroscopic and redox data. The luminescent properties of the complexes were also studied. The complexes showed significant antitumour and anti-HIV activities.