Browsing by Author "R.D. Gupta"

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Effect of dietary protein composition on hepatic protein and enzymes (arginase tyrosine amino transferase and lactic dehydrogenase) in young rats
(1984) R.D. Gupta; K.N. Agarwal; O.P. Malhotra
[No abstract available]
Effect of dietary protein composition on placental protein, nucleic acid, free α amino N and enzymes in rats
(1980) R.D. Gupta; K.N. Agarwal
Pregnant rats were fed 6 different diets from the first to the 15th, 17th or 19th day of pregnancy. Diets 1 to 5 contained the same amount of nitrogen (10% casein and unsupplemented or supplemented wheat or Bengalgram diets). Diet 6 contained 20% casein. Total placental protein, RNA, free α amino N contents and the activities of the enzymes arginase (EC 3.5.3.1), tyrosine amino transferase (TAT, EC 2.6.1.5) and lactate dehydrogenase (LDH, EC 1.1.1.27) were estimated. The fetal weight and placental weight, total placental protein, RNA and free α amino N and the activities of the enzymes increased with the gestational age, but the DNA content became constant after day 17 of gestation. The placental weight, protein, free α amino N and RNA contents were significantly reduced on wheat and Bengalgram diets as compared to 10% casein (control) diet. The low activities of arginase, TAT and LDH on these diets indicated impaired protein synthesis, as a result of reduction in the amino acid pool size. The fortification of wheat with lysine and Benglagram with cystine, methionine and tryptophan showed significant improvement in the fetal weight and placental parameters. The values on the 20% casein diet were significantly higher than those observed on the 10% casein diet.