Browsing by Author "Radha Singh"

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Morpho-physiological attributes of field pea (Pisum sativum L.) genotypes as influenced by salicylic acid under salinity stress
(2013) Radha Singh; A. Hemantaranjan; Pradeep K. Patel; Dinesh K. Yadav
Salinity is major environmental factor that limits productivity of pulse crops all over the world. Field pea (Pisum sativum L.), one of the most important grain legume crops, is highly sensitive to salinity stress. Salinity stress adversely affects the morpho-physiological parameters, viz., germination, root length, plant height, number of leaves, leaf area, and total dry weight at vegetative growth stage of plant. In this investigation, salinity was induced in soil by the application of NaCl @ 50 mM, 100 mM and 150 mM in one shock followed by measurement of electrical conductivity (EC) for growing two field pea genotypes, viz., DDR 61, HUDP 15. Prior to sowing, seeds of both the field pea genotypes were hardened with salicylic acid (SA) @ 1.0 mM for 6 hours with a view to alleviating the harmful effect of salinity stress on morpho-physiological parameters of plants.
Salicylic acid improves salinity tolerance in field pea (Pisum sativum L.) by intensifying antioxidant defense system and preventing salt-induced nitrate reductase (NR) activity loss
(Agricultural Research Communication Centre, 2015) Radha Singh; A. Hemantaranjan; Pradeep Kumar Patel
To improve the antioxidant system and protect the nitrate reductase (NR) activity of two field pea genotypes (DDR 61 and HUDP 15) by seed hardening through optimum concentration salicylic acid (SA) @ 1.0 mM under salinity stress condition. Salinity was imposed by NaCl @ 50,100 and 150 mM with their corresponding EC 4.0, 8.2 and 10.6 dSm-1 respectively. Nitrate reductase (NR) activity significantly reduced under stress at the reproductive (i.e. post anthesis) stage but was maintained higher in 1.0 mM SA treated plants upto the level of 100 mM NaCl. In addition to NR, membrane stability index (MSI) also decreased significantly under salinity stress. In DDR 61 MSI was found to be more as compared to HUDP 15. On the other hand, activities of antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) were up-regulated by salinity stress and further enhanced remarkably by 1.0 mM SA treatment. DDR 61 genotype was found to be more responsive to SA application as compared to HUDP 15. Salt stress was found to have more damaging effects during the pre-anthesis phase than the post-anthesis phase of development. Hence, results signify the role of SA in protecting NR metabolic activity along with regulating salinity response of plants. © 2015, Agricultural Research Communication Centre. All rights reserved.