Browsing by Author "Rajeshwari Negi"

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Biodiversity and biotechnological applications of rhizomicrobiome for agricultural, environmental and industrial sustainability
(Elsevier, 2024) Divjot Kour; Simranjeet Kaur; Tanvir Kaur; Babita Sharma; Rajeshwari Negi; Sofia Sharief Khan; Imran Sheikh; Damini Maithani; Harpreet Kour; Seema Ramniwas; Sarvesh Rustagi; Ashok Yadav; Neelam Thakur; Ajar Nath Yadav; Amrik Singh Ahluwalia
A century of incremental research along with the technological advances and need for sustainable agricultural practices, the study of beneficial rhizomicrobiomes has increased. Rhizomicrobiomes have emerged as an important and promising tool for sustainable agriculture. These plant associated microbiomes play a major role in plant growth promotion by direct and indirect mechanisms and are generally referred to as plant growth promoting microbes. Plant growth promoting microbes also act as the stress alleviators for plants by increasing the accumulation of the osmolytes, reducing the inhibitory ethylene concentrations and producing reactive oxygen species scavengers. These beneficial microbes can be applied to the plants as biofertilizers and biopesticides to reduce the use of the harmful chemicals as well as for the amelioration of abiotic stresses. The present chapter describes the functional diversity of the rhizomicrobiomes, their agricultural and environmental applications further highlighting their role in industrial sector. © 2025 Elsevier Inc. All rights reserved.
Biodiversity, mechanisms, and potential biotechnological applications of minerals solubilizing extremophilic microbes: A review
(Open Science Publishers LLP Inc., 2024) Rubee Devi; Tanvir Kaur; Rajeshwari Negi; Babita Sharma; Sohini Chowdhury; Monit Kapoor; Sangram Singh; Sarvesh Rustagi; Sheikh Shreaz; Pankaj Kumar Rai; Ashutosh Kumar Rai; Ashok Yadav; Divjot Kour; Ajar Nath Yadav
The earth’s surface consists of arid, semi-arid, and hyper-arid lands, where life is profoundly challenged by harsh conditions such as temperature fluctuations, water scarcity, high levels of solar radiations, and soil salinity. The harsh environmental conditions pose serious consequences on plant survival, growth, and productivity accessibility of nutrients reduces. To cope with the harsh environments and increase plant productivity, an extremophilic microbe has attracted agriculturists and environmentalists. The extremophilic microbes, adapted to extreme environmental conditions, offer an unexploited reservoir for biofertilizers, which could provide various forms of nutrients and alleviate the stress caused by the abiotic factors in an environment friendly manner. Worldwide, minerals solubilizing extremophilic microbes are distributed in various hotspots and belong to three domains of life including, archaea, bacteria, and eukarya. The minerals solubilizing extremophilic microbes belongs to diverse phyla, namely, Ascomycota, Actinobacteria, Basidiomycota, Bacteroidetes, Crenarchaeota, Deinococcus-Thermus, Euryarchaeota, Firmicutes, and Proteobacteria. Mineral solubilizing extremophilic microbes achieve the mineral solubilization of phosphorus, potassium, zinc, and selenium by secreting special compounds such as organic acid, exopolysaccharides, and different enzymes. Consequently, extremophilic microbes are becoming increasingly important in agriculture, industries and environmental biotechnology as well, paving the way for novel sequencing technologies and “metaomics” methods, including metagenomics, metatranscriptomics, and metaproteomics. The extremophilic microbial diversity and their biotechnological application in agriculture and industrial applications will be a milestone for future needs. The present review deals with biodiversity, mechanisms and potential biotechnological applications of minerals solubilizing extremophilic microbes. © 2024 Rubee Devi, et al.
Bioformulation of mineral solubilizing microbes as novel microbial consortium for the growth promotion of wheat (Triticum aestivum) under the controlled and natural conditions
(Elsevier Ltd, 2024) Rubee Devi; Tanvir Kaur; Rajeshwari Negi; Divjot Kour; Sanjeev Kumar; Ashok Yadav; Sangram Singh; Kundan Kumar Chaubey; Ashutosh Kumar Rai; Sheikh Shreaz; Ajar Nath Yadav
Microbes are a worthwhile organism of the earth that could be formulated as consortium which can be utilized as biofertilizers. Consortium-based bioinoculants or biofertilizers are superior to single strain-based inoculants for sustainable agricultural productivity and increased micronutrient content in yield. The aim of present study was to evaluate the effect of different combinations of beneficial bacteria that are more effective than single-based bioinoculants. The current work focuses on the isolation of rhizospheric microorganisms from various cereals and pseudocereal crops and the development of a single inoculum as well as a bacterial consortium which were evaluated on wheat crop. A total 214 rhizospheric bacteria were sorted out and, screened for mineral solubilizing attributes i.e., phosphorus, potassium, zinc and selenium solubilization. Among all the bacterial isolates, four potential strains exhibiting P, K, Zn and Se-solubilizing attributes were identified with the help of 16S rRNA gene sequencing as Rahnella aquatilis EU-A3Rb1, Erwinia aphidicola EU-A2RNL1, Brevibacillus brevis EU-C3SK2, and Bacillus mycoides EU-WRSe4, respectively. The identified strains formulated as a consortium which were found to improve the plant growth and physiological parameters in comparison to single culture inoculants and control. To the best of our knowledge, the present investigation is the first report that has developed the consortium from bacterial strains Rahnella aquatilis EU-A3Rb1, Erwinia aphidicola EU-A2RNL1, Brevibacillus brevis EU-C3SK2, and Bacillus mycoides EU-WRSe4. A combination of bacterial strains could be used as liquid inoculants for cereal crops growing in mountainous regions. © 2024
Effect of indigenous mineral availing microbial consortia and cattle manure combination for growth of maize (Zea mays L.)
(Springer, 2024) Rubee Devi; Marwa Fadhil Alsaffar; Duraid K.A. AL-Taey; Sanjeev Kumar; Rajeshwari Negi; Babita Sharma; Sangram Singh; Ashutosh Kumar Rai; Sarvesh Rustagi; Ashok Yadav; Tanvir Kaur; Divjot Kour; Ajar Nath Yadav; Amrik Singh Ahluwalia
Plant growth promoting bacterial strains were used as bioinoculants on cereal crops to improve plant growth and plant productivity. Crop responses to inoculation are complex because bacteria are not compatible with each other. Therefore, it is necessary to increase our understanding of the microbial ecology of crop rhizosphere under various agricultural techniques. In tropical agriculture, cattle manure is used as an organic fertilizer to increase soil fertility, however use of microbes as consortium have found as sustainable method for the enhancement of crops productivity. The purpose of this study was to evaluate the effects of three potential plant growth-promoting rhizospheric and endophytic bacterial strains EU-C3ST.R1, IARI-JR-44, and IARI-S-45 and organic fertilizers (cattle manure) individually and as consortia on maize (Zea mays L.) under both in-vitro and in-vivo conditions. A total of 123 bacterial strains were sorted out and screened for nitrogen fixation, phosphorus, and potassium solubilization. The potential N2-fixing; P and K solubilizing bacterial strains were identified using 16 S rRNA gene sequencing as Pseudomonas sp. EU-C3ST.R1, Micrococcus indicus IARI-JR-44, and Bacillus horikoshii IARI-S-45 respectively. The inoculation of these three strains on maize as microbial consortium and individual inoculum significantly increased the growth characteristic including height and biomass of the plants, as well as physiological characteristics i.e., chlorophyll, carotenoids, flavonoids, phenolics, and total soluble sugar content of the plant with respect to chemical fertilizers, cattle manure, and untreated control plant. The consortia were found to be more effective with respect to individual inoculants, cattle manure, and uninoculated control plants, so it can be utilized as biofertilizers for inoculation of cereal crops growing in hilly regions. © The Author(s) under exclusive licence to Society for Plant Research 2024.
Effect of indigenous mineral availing microbial consortia and cattle manure combination for growth of maize (Zea mays L.)
(Springer, 2025) Rubee Devi; Marwa Fadhil Fadhil Alsaffar; Duraid K.A. Altaey; Sanjeev Kumar; Rajeshwari Negi; Babita Sharma; Sangram N. Singh; Ashutosh Kumar Rai; Sarvesh Rustagi; Ashok Yadav; Tanvir Kaur; Divjot KOUR; Ajar Nath Yadav; Amrik Singh Ahluwalia
Plant growth promoting bacterial strains were used as bioinoculants on cereal crops to improve plant growth and plant productivity. Crop responses to inoculation are complex because bacteria are not compatible with each other. Therefore, it is necessary to increase our understanding of the microbial ecology of crop rhizosphere under various agricultural techniques. In tropical agriculture, cattle manure is used as an organic fertilizer to increase soil fertility, however use of microbes as consortium have found as sustainable method for the enhancement of crops productivity. The purpose of this study was to evaluate the effects of three potential plant growth-promoting rhizospheric and endophytic bacterial strains EU-C3ST.R1, IARI-JR-44, and IARI-S-45 and organic fertilizers (cattle manure) individually and as consortia on maize (Zea mays L.) under both in-vitro and in-vivo conditions. A total of 123 bacterial strains were sorted out and screened for nitrogen fixation, phosphorus, and potassium solubilization. The potential N2-fixing; P and K solubilizing bacterial strains were identified using 16 S rRNA gene sequencing as Pseudomonas sp. EU-C3ST.R1, Micrococcus indicus IARI-JR-44, and Bacillus horikoshii IARI-S-45 respectively. The inoculation of these three strains on maize as microbial consortium and individual inoculum significantly increased the growth characteristic including height and biomass of the plants, as well as physiological characteristics i.e., chlorophyll, carotenoids, flavonoids, phenolics, and total soluble sugar content of the plant with respect to chemical fertilizers, cattle manure, and untreated control plant. The consortia were found to be more effective with respect to individual inoculants, cattle manure, and uninoculated control plants, so it can be utilized as biofertilizers for inoculation of cereal crops growing in hilly regions. © The Author(s) under exclusive licence to Society for Plant Research 2024.
Endophytic nitrogen-fixing bacteria: Untapped treasurer for agricultural sustainability
(Open Science Publishers LLP Inc., 2023) Kusam Lata Rana; Divjot Kour; Tanvir Kaur; Rajeshwari Negi; Rubee Devi; Neelam Yadav; Pankaj Kumar Rai; Sangram Singh; Ashutosh Kumar Rai; Ashok Yadav; R.Z. Sayyed; Ajar Nath Yadav
Nitrogen (N) is one of the vital elements required for proper growth and development of plants. In the earth’s atmosphere, N is available in the form of nitrogen gas (N2) and mostly plants utilize N in the form nitrate (NO3-) and ammonium ion (NH4+) which are fixed through the biological process known as N2 fixation. As N is one of the elements most likely to be limiting to plant growth, this phenomenon provides an alternative to the implementations of chemical fertilizers as source of nutrients which have resulted in the ammonia volatilization, leading to significant impact on global warming in the atmosphere which, further, diverts the focus of scientist to find out eco-friendly technology. Globally, the demand for introducing eco-friendly practices for improving sustainable agriculture productivity has been increased. Since long time, microbes play an important role in providing pollution-free environment. Endophytic microbes being present inside the specific tissues of plants mostly empower in the growth of plants. The endophytic nitrogen-fixing microbe has been well characterized from leguminous as well non-legume crops. Endophytic bacteria belong to different phyla such as Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. The predominant N2-fixing endophytic Burkholderia, Rhizobium, Pseudomonas, Bradyrhizobium, Bacillus, Frankia, Enterobacter, and Azospirillum have been reported from different host plant. Nitrogen-fixing endophytic bacteria has a wide variety of application for maintaining growth of plant, crop yield, and health of soil for sustainable agriculture. The present review focuses on major developments on biodiversity of N-fixing endophytic microbiomes and their role for plant growth promotion and soil health for agroenvironmental sustainability. © 2023 Kusam Lata Rana, et al.
Fungi as an unseen heritage and wealth: Conclusion and future challenges
(Elsevier, 2024) Ajar Nath Yadav; Tanvir Kaur; Rubee Devi; Rajeshwari Negi; Divjot Kour; Ashok Yadav; Ait Bessai Sylia; Ashutosh Kumar Rai; Elhafid Nabti; Neelam Yadav; Ahmed M. Abdel-Azeem; Amrik Singh Ahluwalia
Endophytic fungi are largely recognized for their wide range of applications. They are known to be associated with a wide range of plants, both land- and water-based. Endophytic fungi residing inside plant tissues are very diverse and belong to phyla such as Ascomycota, Basidiomycota, and Mucoromycota. Endophytic fungi are a key source of natural compounds such as enzymes, secondary metabolites, and bioactive compounds, and these have several applications in human health as well as the agricultural and industrial sectors. Fungal endophytes are also utilized as bioinoculants to promote plant growth. Fungal endophytes show different plant growth-promoting abilities such as the solubilization of minerals as well as the production of phytohormones, siderophores, ammonia, and hydrogen cyanide that alleviate stress caused by nutrient depletion as well as environmental factors such as drought, salinity, flooding, and heavy metals. Endophytic fungi also play a pivotal role as biocontrol agents that help control pests and pathogens attacking various plants. In this chapter, their biodiversity, roles, and applications in various sectors are discussed in detail. © 2024 Elsevier Inc. All rights are reserved including those for text and data mining AI training and similar technologies.
Microbes Mediated Nutrient Dynamics for Plant Growth Promotion: Current Research and Future Challenges
(Springer, 2024) Rubee Devi; Tanvir Kaur; Rajeshwari Negi; Babita Sharma; Sanjeev Kumar; Sangram Singh; Ashutosh Kumar Rai; Sarvesh Rustagi; Ashok Yadav; Anu Kumar; Divjot Kour; Ajar Nath Yadav
On earth, soil is one of the most essential parts of nature which plays critical roles in plant growth, water flow, waste products recycling and provides habitats to various organisms. Soil is the combination of organic matter, air, water minerals, and sixteen different essential nutrient elements which are categorized into primary macronutrients, secondary macronutrients, and micronutrients. The nutrients elements present in soil either in organic forms or organic forms interchanged by the various microbial mechanisms such including fixation, chelation and solubilization. The microbes from all three domain i.e., archaea, bacteria, and eukarya have been reported for exhibiting the various mechanisms and strain belonging to genera Arthrobacter, Burkholderia, Bacillus, Paenibacillus, Pseudomonas, Rhizobium, Natrinema, and Serratia are widely known for ruling the nutrients dynamics. The microbes playing role in nutrients dynamics, have great economic importance in agriculture sector as agriculturist is in pressure of producing high quality and quantity of food along with managing the sustainability. These microbes could solve agricultural problems such as soil degradation and environmental pollution by using them as bio-fertilizer over chemical-based products. A huge number of reports have supported such statements so, the purpose of the present review aims to complies microbial role in all category nutrients dynamics and their role in plant growth promotion. © Association of Microbiologists of India 2024.
Microbes Mediated Nutrient Dynamics for Plant Growth Promotion: Current Research and Future Challenges
(Springer, 2025) Rubee Devi; Tanvir Kaur; Rajeshwari Negi; Babita Sharma; Sanjeev Raj Kumar; Sangram N. Singh; Ashutosh Kumar Rai; Sarvesh Rustagi; Ashok Yadav; Anu Kumar; Divjot KOUR; Ajar Nath Yadav
On earth, soil is one of the most essential parts of nature which plays critical roles in plant growth, water flow, waste products recycling and provides habitats to various organisms. Soil is the combination of organic matter, air, water minerals, and sixteen different essential nutrient elements which are categorized into primary macronutrients, secondary macronutrients, and micronutrients. The nutrients elements present in soil either in organic forms or organic forms interchanged by the various microbial mechanisms such including fixation, chelation and solubilization. The microbes from all three domain i.e., archaea, bacteria, and eukarya have been reported for exhibiting the various mechanisms and strain belonging to genera Arthrobacter, Burkholderia, Bacillus, Paenibacillus, Pseudomonas, Rhizobium, Natrinema, and Serratia are widely known for ruling the nutrients dynamics. The microbes playing role in nutrients dynamics, have great economic importance in agriculture sector as agriculturist is in pressure of producing high quality and quantity of food along with managing the sustainability. These microbes could solve agricultural problems such as soil degradation and environmental pollution by using them as bio-fertilizer over chemical-based products. A huge number of reports have supported such statements so, the purpose of the present review aims to complies microbial role in all category nutrients dynamics and their role in plant growth promotion. © Association of Microbiologists of India 2024.
Plant endophytes: unveiling hidden applications toward agro-environment sustainability
(Springer Science and Business Media B.V., 2024) Rajeshwari Negi; Babita Sharma; Sanjeev Kumar; Kundan Kumar Chaubey; Tanvir Kaur; Rubee Devi; Ashok Yadav; Divjot Kour; Ajar Nath Yadav
Endophytic microbes are plant-associated microorganisms that reside in the interior tissue of plants without causing damage to the host plant. Endophytic microbes can boost the availability of nutrient for plant by using a variety of mechanisms such as fixing nitrogen, solubilizing phosphorus, potassium, and zinc, and producing siderophores, ammonia, hydrogen cyanide, and phytohormones that help plant for growth and protection against various abiotic and biotic stresses. The microbial endophytes have attained the mechanism of producing various hydrolytic enzymes such as cellulase, pectinase, xylanase, amylase, gelatinase, and bioactive compounds for plant growth promotion and protection. The efficient plant growth promoting endophytic microbes could be used as an alternative of chemical fertilizers for agro-environmental sustainability. Endophytic microbes belong to different phyla including Euryarchaeota, Ascomycota, Basidiomycota, Mucoromycota, Firmicutes, Proteobacteria, and Actinobacteria. The most pre-dominant group of bacteria belongs to Proteobacteria including α-, β-, γ-, and δ-Proteobacteria. The least diversity of the endophytic microbes have been revealed from Bacteroidetes, Deinococcus-Thermus, and Acidobacteria. Among reported genera, Achromobacter, Burkholderia, Bacillus, Enterobacter, Herbaspirillum, Pseudomonas, Pantoea, Rhizobium, and Streptomyces were dominant in most host plants. The present review deals with plant endophytic diversity, mechanisms of plant growth promotion, protection, and their role for agro-environmental sustainability. In the future, application of endophytic microbes have potential role in enhancement of crop productivity and maintaining the soil health in sustainable manner. © Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2023.
Potential effect of novel endophytic nitrogen fixing diverse species of Rahnella on growth promotion of wheat (Triticum aestivum L.)
(Springer, 2024) Kusam Lata Rana; Rajeshwari Negi; Babita Sharma; Ashok Yadav; Rubee Devi; Tanvir Kaur; Sheikh Shreaz; Sarvesh Rustagi; Ashutosh Kumar Rai; Sangram Singh; Divjot Kour; Ajar Nath Yadav
The present investigation aims to isolate nitrogen fixing endophytic bacteria from cereals crops and their potential role in plant growth promotion of wheat (Triticum aestivum L.) for sustainable growth. In the present investigation, endophytic bacteria were isolated from different cereal crops growing in the Divine Valley of Baru Sahib, Himachal Pradesh, India and isolates were screened for nitrogen fixation. The nitrogenase activity exhibiting bacterial isolates were further screened for other plant growth promoting traits including solubilization of phosphorus, potassium, and zinc; production of indole-3-acetic acid, siderophores, ammonia, hydrogen cyanide and extracellular enzyme. The potential nitrogen fixing strains were molecularly identified and evaluated for the growth promotion of wheat. A total of 304 putative endophytic bacterial isolates were isolated from wheat, oats, barley, and maize using selective and complex growth media. Among 304 putative endophytic bacteria, 8 isolates exhibits nitrogenase activity. On the basis of nitrogenase activity and other plant promoting traits, two efficient strains i.e. EU-E1ST3.1 and EU-A2RNfb were molecularly identified using 16S rRNA gene sequencing and found that these strains belongs to genera Rahnella. The wheat inoculated with two selected nitrogen-fixing endophytic bacterial strains showed considerable enhancement in total chlorophyll, nitrogen, Fe and Zn content over the un-inoculated control. In comparison of two selected nitrogen-fixing endophytic bacterial strains, Rahnella aquatilis EU-E1ST3.1 was found to enhance better growth and physiological parameters and it might be developed as biofertilizers to establish a sustainable agriculture system. In the present investigation, the isolated potential nitrogen fixing endophytic bacteria could be used as biofertilizer or bioinoculant for growth of diverse cereal crops growing in hilly region for agricultural sustainability. © The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS) 2024.
Rhizomicrobiome: Biodiversity and functional annotation for agricultural sustainability
(Elsevier, 2024) Sofia Sharief Khan; Babita Sharma; Rajeshwari Negi; Simranjeet Kaur; Tanvir Kaur; Damini Maithani; Imran Sheikh; Harpreet Kour; Seema Ramniwas; Ashok Yadav; Divjot Kour; Neelam Thakur; Ajar Nath Yadav; Shafaq Rasool; Amrik Singh Ahluwalia
The rhizomicrobiome, which consists of a rich and diverse assemblage of microbial organisms, functions analogously to the gut microbiome in the context of the plant genome. This resemblance can be attributed to the presence of microbial communities within the human gut. The habitat in question exhibits exceptional qualities, serving as a refuge for a remarkably diverse microbial community. Even though many studies have revealed the enormous functional versatility of rhizomicrobiome communities, our knowledge of the specific processes underlying the influence of rhizomicrobiome assemblies is still in its infancy. Furthermore, little is understood about the numerous advantageous properties of the rhizomicrobiome. An urgent need arises for a comprehensive and holistic investigation aimed at enhancing our comprehension of the dynamics of microbial communities, specifically the diversity and function of soil microbiomes. This study focuses on exploring this community's diversity and understanding the rhizomicrobiome role as biofertilizers, phytostimulators, stress alleviators, and biocontrol agents. © 2025 Elsevier Inc. All rights reserved.