Browsing by Author "Rajib Roychowdhury"

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Advancement of Abiotic Stresses for Microalgal Lipid Production and Its Bioprospecting into Sustainable Biofuels
(Multidisciplinary Digital Publishing Institute (MDPI), 2023) Rahul Prasad Singh; Priya Yadav; Indrajeet Kumar; Manoj Kumar Solanki; Rajib Roychowdhury; Ajay Kumar; Rajan Kumar Gupta
The world is currently facing global energy crises and escalating environmental pollution, which are caused by the extensive exploitation of conventional energy sources. The limited availability of conventional energy sources has opened the door to the search for alternative energy sources. In this regard, microalgae have emerged as a promising substitute for conventional energy sources due to their high photosynthetic rate, high carbohydrate and lipid content, efficient CO2 fixation capacity, and ability to thrive in adverse environments. The research and development of microalgal-based biofuel as a clean and sustainable alternative energy source has been ongoing for many years, but it has not yet been widely adopted commercially. However, it is currently gaining greater attention due to the integrated biorefinery concept. This study provides an in-depth review of recent advances in microalgae cultivation techniques and explores methods for increasing lipid production by manipulating environmental factors. Furthermore, our discussions have covered high lipid content microalgal species, harvesting methods, biorefinery concepts, process optimizing software tools, and the accumulation of triglycerides in lipid droplets. The study additionally explores the influence of abiotic stresses on the response of biosynthetic genes involved in lipid synthesis and metabolism. In conclusion, algae-based biofuels offer a viable alternative to traditional fuels for meeting the growing demand for energy. © 2023 by the authors.
Plant-Endophyte Interaction during Biotic Stress Management
(MDPI, 2022) Parul Pathak; Vineet Kumar Rai; Hasan Can; Sandeep Kumar Singh; Dharmendra Kumar; Nikunj Bhardwaj; Rajib Roychowdhury; Lucas Carvalho Basilio de Azevedo; Kaushalendra; Hariom Verma; Ajay Kumar
Plants interact with diverse microbial communities and share complex relationships with each other. The intimate association between microbes and their host mutually benefit each other and provide stability against various biotic and abiotic stresses to plants. Endophytes are heterogeneous groups of microbes that live inside the host tissue without showing any apparent sign of infection. However, their functional attributes such as nutrient acquisition, phytohormone modulation, synthesis of bioactive compounds, and antioxidant enzymes of endophytes are similar to the other rhizospheric microorganisms. Nevertheless, their higher colonization efficacy and stability against abiotic stress make them superior to other microorganisms. In recent studies, the potential role of endophytes in bioprospecting has been broadly reported. However, the molecular aspect of host–endophyte interactions is still unclear. In this study, we have briefly discussed the endophyte biology, colonization efficacy and diversity pattern of endophytes. In addition, it also summarizes the molecular aspect of plant–endophyte interaction in biotic stress management. © 2022 by the authors.
Recent advances in biotechnology and bioengineering for efficient microalgal biofuel production
(Elsevier B.V., 2025) Chaoqun Zhang; Rahul Prasad Singh; Priya Yadav; Indrajeet Anil Kumar; Amit Kaushik; Rajib Roychowdhury; Mustansar Mubeen; Sandeep Kumar Singh; Ajay Kumar; Jie Wang
Microalgal biofuels have emerged as a promising avenue for meeting the growing demands for clean and efficient energy. However, the integration of microalgae into the biofuel industry is still in the early stages, primarily due to low productivity and high production costs. To address these challenges, researchers are actively exploring innovative methods to enhance biomass, concurrently increasing lipid and carbohydrate content. This review paper discusses the unique attributes of microalgae that make them attractive candidates for biofuel production. Advancements in cultivation techniques, such as photobioreactor design, co-cultivation strategies (microalgae-microalgae, microalgae-bacteria, and microalgae-fungi), and the optimization of nutrient conditions (carbon, nitrogen, and phosphorus) as well as environmental factors (salinity, light, and temperature) were explored to enhance biomass and lipid productivity. Furthermore, genetic engineering tools (genetic elements, gene interference, genome editing, and genome reconstruction) and omics technologies (genomics, transcriptomics, and proteomics) were discussed to gain a deeper understanding of microalgal lipid synthesis metabolism. The application of these techniques in microalgae facilitates enhanced lipid productivity, improved stress tolerance, optimized carbon sequestration and utilization, and reduced harvesting and processing costs. The study also delves into the decision-making process related to software selection, with the overarching goal of improving performance, profitability, and sustainability while mitigating risks, operational costs, and environmental impacts. Additionally, this review highlights future perspectives on large-scale microalgal biofuel production and its industry. © 2025 The Author(s)