Browsing by Author "Rakesh Maurya"

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Anti-ulcer constituents of Annona squamosa twigs
(2011) Dinesh K. Yadav; Neetu Singh; Kapil Dev; Rolee Sharma; Mahendra Sahai; Gautam Palit; Rakesh Maurya
Phytochemical investigation of Annona squamosa twigs, resulted in isolation and identification of twelve known (1-12) compounds among them one 1-(4-β-D-glucopyranosyloxyphenyl)-2-(β-D-glucopyranosyloxy)-ethane (11) is synthetically known but first time isolated from natural sources. Their structures were elucidated using 1D and 2D NMR spectroscopic analysis. The isolated compounds (2-8, 11) were evaluated for H+ K +-ATPase activity. Three of these compounds (+)-O-methylarmepavine (2), N-methylcorydaldine (3), isocorydine (6) showed promising anti-secretory activity. Activity of these compounds, comparable to the standard drug omeprazole is novel to our finding. Moreover, there is no information accessible regarding the pharmacological effect of A. squamosa on the gastrointestinal system. This study is the first of its kind to show the significant anti-ulcer effect of A. squamosa. The present study aimed to evaluate the gastroprotective effect of A. squamosa (AS) and to identify its active constituents. Anti-ulcer activity was evaluated against cold restraint (CRU), pyloric ligation (PL), aspirin (ASP), alcohol (AL) induced gastric ulcer and histamine (HA) induced duodenal ulcer model and further confirmed through in vitro assay of H + K+-ATPase activity and plasma gastrin level. AS and its chloroform and hexane fraction attenuated ulcer formation in CRU, PL, HA model and displayed anti-secretory activity in vivo through reduced free, total acidity and pepsin in PL, confirmed by in vitro inhibition of H+ K+-ATPase activity with corresponding decrease in plasma gastrin level. Cytoprotection of AS was apparent with protection in AL, ASP models and enhanced mucin level in PL. © 2011 Elsevier B.V. All rights reserved.
Development of ultra performance liquid chromatography tandem mass spectrometry method for simultaneous identification and quantitation of potential osteogenic phytochemicals in butea monosperma
(Oxford University Press, 2018) Vikas Bajpai; Awantika Singh; Pratibha Singh; Khushbu Sharma; Bikarma Singh; Bhim Pratap Singh; Mahendra Sahai; Rakesh Maurya; Brijesh Kumar
An ultra performance liquid chromatography coupled with hybrid triple-quadrupole linear ion trap tandem mass spectrometry (UPLC-ESI-QqQLIT-MS-MS) method in multiple reaction monitoring mode was developed for identification and simultaneous determination of potential osteogenic compounds in ethanol extracts of different plant parts of Butea monosperma collected from different geographical regions. The chromatographic separation was carried out on an Acquity UPLC CSH C18 column (1.7 μm, 2.1 × 100 mm) with 0.1% (v/v) formic acid in water and methanol as mobile phase under gradient conditions in 8 min. The developed method was validated according to the guidelines of international conference on harmonization. The correlation coefficients of all the calibration curves were ≥0.9995 and recoveries ranged from 95.2 to 105.8% (RSD ≤ 1.95%). Relative standard deviations of intra-day, inter-day precisions and stability were ≤1.74, 1.84 and 2.8%, respectively. The quantitative results showed remarkable differences in the content of all potential osteogenic compounds in different parts of the plant as well as samples from different geographical regions. Quantitative variations studied from principal component analysis indicated tentative markers for B. monosperma cultivars which can discriminate sample of different geographical regions. © The Author(s) 2018. Published by Oxford University Press. All rights reserved.
Effect of nitrogen levels on growth attributes, yield and nutrient uptake of different rice (Oryza sativa l.) varieties under the transplanted condition
(Editorial board of Journal of Experimental Biology and Agricultural Sciences, 2021) Rakesh Maurya; Manoj Kumar Singh; Nikhil Kumar Singh; Manish Kumar Singh; Anurag Kumar Singh
Rice is very responsive to nitrogen fertilization under transplanted conditions; however N use efficiency and uptake by different varieties may vary. A field study was conducted during the rainy season of 2017 to assess the effect of four nitrogen levels in three rice varieties under the transplanted condition at Agricultural Research Farm, Banaras Hindu University, Varanasi, Uttar Pradesh. The experiment was carried out in a split-plot design (SPD) with three replications. The total number of treatments was twelve involving three rice varieties (V1-BPT-5204, V2-Rajendra Kasturi and V3-HUBR 2-1) in main plots and four nitrogen levels (N1-100, N2-120, N3-140, and N4-160 kg ha-1) in sub-plots. Rice variety BPT-5204 recorded significantly higher growth parameters viz. leaf number hill-1(37.17), SPAD value (chlorophyll content 31.83), the higher number of days taken to 50 percent flowering (103 days), maturity (130.17 days), grain yield (50.73 q ha-1), biological yield (112.90 q ha-1) and harvest index (0.45) as well as NPK uptakes by grain (60.96, 13.01 & 10.95 kg ha-1) and straw (29.72, 7.15 & 101.55 kg ha-1) as compared to other varieties. However, straw yield (62.17 q ha-1) was reported higher under HUBR 2-1 as compared to the rest varieties. Among the nitrogen levels, N4-160 kg N ha-1 was recorded significantly higher above the same parameter as compared to other nitrogen levels. Hence, the application of N @ 160 kg ha-1 along with rice variety BPT-5204 can be recommended for achieving higher rice yield, nutrient uptake, and efficiency in Eastern Uttar Pradesh. © 2021, Editorial board of Journal of Experimental Biology and Agricultural Sciences. All rights reserved.
Immunomodulatory constituents from annona squamosa twigs provoke differential immune response in BALB/c mice
(2013) Vishal Kumar Soni; Manisha Pathak; Dinesh Kumar Yadav; Rakesh Maurya; Mahendra Sahai; Swatantra Kumar Jain; Shailja Misra-Bhattacharya
Annona squamosa (AS) has traditionally been used as ethnomedicine and various parts of the plant have been used to combat several disorders including dysentery, cancer and hyperthyroidism. Since the twig of this plant is reported to contain a large number of alkaloids, we chose to study its medicinal properties on the immune response of BALB/c mice. The present study, thus, aims at evaluation of immunomodulatory activity in the crude ethanolic extract and its four fractions, viz. hexane (F1), chloroform (F2), n-butanol (F3) and aqueous (F4) prepared from the twigs of AS to locate the active constituents in the fractions. The extract and fractions were fed orally at 3, 10 and 30 mg/kg for 14 consecutive days and mice were euthanized to assess various immune parameters. The ethanolic extract and its three fractions F2, F3 and F4 were found active since they increased splenic T and B cellular proliferation with a significant accentuation in peritoneal macrophage function, differentially increased the CD4+, CD8+ T lymphocytes and CD19+ B lymphocytes. The extract and its active fractions also demonstrated significant Th1 or Th2 mixed cytokine response at almost all doses tried in a dose-dependent manner. Its hexane fraction, however, could only induce reactive oxygen species production in peritoneal macrophages and could not induce lymphocytes; thus, it remained inactive. Thus, the activity could be localized distributed in its three fractions (chloroform, n-butanol and aqueous). Further purification and evaluation of the active molecule/s is underway in our laboratory.
N-methyl-6, 7-dimethoxyisoquinolone in Annona squamosa twigs is the major immune modifier to elicit polarized Th1 immune response in BALB/c mice
(2012) Vishal Kumar Soni; Dinesh Kumar Yadav; Nasreen Bano; Preety Dixit; Manisha Pathak; Rakesh Maurya; Mahendra Sahai; Swatantra Kumar Jain; Shailja Misra-Bhattacharya
Annona squamosa (AS) has traditionally been used as ethnomedicine. We have earlier extracted and fractionated the twigs of AS based upon its bioactivity and observed its immune potentiating activity that was localized in its three fractions. Present communication deals with the phytochemical analysis and pharmacological investigation of the most active chloroform fraction that led to isolation and identification of a number of compounds whose structures were elucidated using 1D and 2D NMR spectroscopic analysis. Amongst the twelve pure compounds isolated, five compounds Lanuginosine (1), (+) -O- methylarmepavine (2), (+)-anomuricine (3), Isocorydine (4), and N-methyl-6, 7- dimethoxyisoquinolone (5) were evaluated in vivo for their immune modifier activities in BALB/c mice after oral administration at three log doses of 0.3, 1.0 and 3.0 mg/kg for 14 consecutive days. Of these, three compounds (1, 2 and 5) showed dose dependent immune stimulating activity. However, the uppermost activity was noted in the compound N-methyl-6, 7-dimethoxyisoquinolone at the 3.0 mg/kg oral dose. The activity was assessed in the form of increased splenic T and B cellular proliferation, up-regulated CD4+, CD8+ and CD19+ cell population and accentuation in the peritoneal macrophage function. The compound possibly acted modifying the expression of Th1- and Th2- cytokines via stimulation of pro-inflammatory Th1 cytokines IL-2 and IFN-γ. These results warrant the use of the above compounds as an efficient immune-stimulant or immune-adjuvant against diseases with immune suppression. The analogs of the compound may further be chemically synthesized to achieve desired immune modifying activity. Crown Copyright © 2011 Published by Elsevier B.V. All rights reserved.
New lignan glycosides from Cissus quadrangularis stems
(Taylor and Francis Ltd., 2019) Padam Kumar; Kapil Dev; Khushbu Sharma; Mahendra Sahai; Rakesh Maurya
Phytochemical investigation of Cissus quadrangularis stems led to the isolation of one new phenolic glycoside (1) and two new lignan glycosides (7 & 8) along with twelve known compounds (2–6 & 9–15). Their chemical structures were determined on the basis of extensive spectroscopic analysis using 1D, 2D NMR, and mass spectrometric analysis. Among the known compounds, 4–6, 9 and 12 were isolated for the first time from the genus Cissus whereas compounds 10, 11 and 13 for the first time from this plant. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Osteogenic activity of constituents from Butea monosperma
(2009) Rakesh Maurya; Dinesh K. Yadav; Geetu Singh; Biju Bhargavan; P.S. Narayana Murthy; Mahendra Sahai; Man Mohan Singh
Phytochemical investigation from the stem bark of Butea monosperma, led to the isolation and identification of three new compounds named buteaspermin A (1), buteaspermin B (2) and buteaspermanol (3), along with 19 known compounds. The structure of compounds 1-22 were established on the basis of their spectroscopic data. The isolated compounds 2-17 were evaluated using neonatal (1-3 day old) rat calvaria derived primary osteoblast cultures. Five of these compounds 7, 10-13 showed promising osteogenic activity, attributed to increased osteoblast proliferation, differentiation and mineralization as evidenced by marked increase in expression of alkaline phosphatase, an early phase differentiation marker, and alizarin Red S staining of osteoblasts cultured for 48 h and von Kossa silver staining of nodules formed 15 days after culture with these compounds. Quantification of mineralization by optical density measurement of Alizarin Red S extracted from stained osteoblasts cultured for 7 days in presence of these compounds showed significant (P < 0.05, vs corresponding vehicle control group) increase in mineralization. On the basis of biological results, structure-activity relationships are discussed. © 2009 Elsevier Ltd. All rights reserved.
Performance of various rice (Oryza sativa l.) varieties under variable nitrogen levels in the eastern uttar pradesh
(Editorial board of Journal of Experimental Biology and Agricultural Sciences, 2021) Rakesh Maurya; Manoj Kumar Singh; Mona Nagargade; Deepak Kumar Yadav; Vijay Pratap
A field experiment was conducted at the Indo Gangetic plain of Varanasi in 2017 during the rainy season. The study was conducted in a split-plot design with three main plots treatments viz. three rice varieties (V1-BPT-5204, V2-Rajendra Kasturi and V3-HUBR 2-1) and four subplots treatments viz. nitrogen levels (N1-100, N2-120, N3-140 and N4-160 kg ha-1). Each treatment was replicated three times. The results of the study revealed that the BPT-5204 variety had significantly higher growth attributes as compared to the other two varieties, including plant height (104.92 cm), leaf area index (5.26), tiller hill-1 (8.25), and dry matter production (46.59 g hill-1), similarly yield and yield parameters were highest in BPT-5204 rice variety viz. the number of panicles m-2 (352.50), grains panicle-1 (177.08), panicles weight (3.51g), panicle length (23.67 cm), grain yield (50.73q ha-1), biological yield (112.90 q ha-1) and harvest index (0.45) but HUBR 2-1 variety gave significantly higher straw yields (62.17q ha-1) and test weight (20.91 g). For the subplots treatments, nitrogen (N) level 160 kg ha-1 gave the significantly highest growth parameters, yield and yield parameters. © 2021, Editorial board of Journal of Experimental Biology and Agricultural Sciences. All rights reserved.
Synthetic analogs of daidzein, having more potent osteoblast stimulating effect
(2011) Dinesh K. Yadav; Abnish K. Gautam; Jyoti Kureel; Kamini Srivastava; Mahendra Sahai; Divya Singh; Naibedya Chattopadhyay; Rakesh Maurya
A series of didzein derivatives were synthesized and assessed for stimulation of osteoblast differentiation using primary cultures of rat calvarial osteoblasts. Data suggested that three synthetic analogs, 1c, 3a and 3c were several folds more potent than daidzein in stimulating differentiation and mineralization of osteoblasts. Further, these three compounds did not show any estrogen agonistic activity, however had mild estrogen antagonistic effect. Out of the three compounds, 3c was found to maximally increase the mineralization of bone marrow osteoprogenitor cells. Compound 3c also robustly increased the mRNA levels of osteogenic genes including bone morphogenetic protein-2 and osteocalcin in osteoblasts. Unlike daidzein, 3c did not inhibit osteoclastogenesis. Collectively, we demonstrate osteogenic activity of daidzein analogs at significantly lower concentrations than daidzein. © 2010 Elsevier Ltd. All rights reserved.
Tinosporaside from Tinospora cordifolia Encourages Skeletal Muscle Glucose Transport through Both PI-3-Kinase- and AMPK-Dependent Mechanisms
(MDPI, 2023) Akansha Mishra; Khushbu Sharma; Jyotsana Pandey; Kapil Dev; Sleman Kadan; Mahendra Sahai; Ishbal Ahmad; Arvind K. Srivastava; Akhilesh K. Tamrakar; Hilal Zaid; Rakesh Maurya
The stem of Tinospora cordifolia has been traditionally used in traditional Indian systems of medicine for blood sugar control, without the knowledge of the underlying mechanism and chemical constitution responsible for the observed anti-diabetic effect. In the present study, Tinosporaside, a diterpenoid isolated from the stem of T. cordifolia, was investigated for its effects on glucose utilization in skeletal muscle cells, which was followed by determining the anti-hyperglycemic efficacy in our diabetic db/db mice model. We found that tinosporaside augmented glucose uptake by increasing the translocation of GLUT4 to the plasma membrane in L6 myotubes, upon prolonged exposure for 16 h. Moreover, tinosporaside treatment significantly increased the phosphorylation of protein kinase B/AKT (Ser-473) and 5′ AMP-activated protein kinase (AMPK, Thr-172). These effects were abolished in the presence of the wortmannin and compound C. Administration of tinosporaside to db/db mice improved glucose tolerance and peripheral insulin sensitivity associated with increased gene expression and phosphorylation of the markers of phosphoinositide 3-kinases (PI3Ks) and AMPK signaling in skeletal muscle tissue. The findings revealed that tinosporaside exerted its antidiabetic efficacy by enhancing the rate of glucose utilization in skeletal muscle, mediated by PI3K- and AMPK-dependent signaling mechanisms. © 2023 by the authors.