Browsing by Author "Ramwant Kumar Gupta"

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Identification and characterization of Eco-miR 169-EcNF-YA13 gene regulatory network reveal their role in conferring tolerance to dehydration and salinity stress in finger millet
(Nature Research, 2025) Varsha Rani; Sumi Rana; Mehanathan Muthamilarasan; Dinesh Chandra Joshi; Ramwant Kumar Gupta; Rajesh Kumar Singh; D. L. Yadav
The finger millet (Eleusine coracana (L.) Gaertn) genome, comprised 166 conserved microRNAs (miRNAs) belonging to 39 families and three novel miRNAs. The miR169 is one of the most conserved miRNA families, while Eco_N1 is a species-specific miRNA prevalent in finger millet. Its members regulate the expression of genes encoding the Nuclear Factor-Y subunit A (NF-YA) via transcript cleavage. However, the role of miRNA genes in regulating the expression of NF-YA transcription factors in finger millet needs to be deciphered. The present study characterized 166 conserved and novel miRNAs (Eco_N1, Eco_N2 and Eco_N3). Further, secondary structures were predicted, and the potential miR genes targeting the NF-YA transcription factors regulating abiotic stress tolerance were analysed. Twenty-three Eco-miR169 members and one Eco_N1 miRNA targeting EcNF-YA13 were identified in the finger millet genome. The presence of relevant cis-elements such as ABRE (abscisic acid-responsive elements), DRE (dehydration-responsive element), and MYB (myeloblastosis) indicates that the target of Eco-miR169 might be involved in abiotic stress responses. The tissue-specific RNA-seq transcriptomic expression pattern of Eco-miR169 showed variable fold of expression in seedlings compared to the control. At the same time, the expression of EcNF-YA13 (target genes of Eco-miR169 members and Eco_N1) presented a downregulated trend under salinity and dehydration conditions compared to the control. Tissue-specific RNA-seq followed by expression analysis confirmed the antagonistic effect of Eco-miR genes on EcNF-YA13. In a nutshell, the results of this study could be utilized as a platform for further exploration and characterization of finger millet Eco-miR169-EcNF-YA13gene regulatory network. © The Author(s) 2025.
Regulation of Transcription Factors in Abscisic Acid-Mediated Signaling Under Abiotic Stresses
(CRC Press, 2025) Varsha Rani; Ramwant Kumar Gupta; Manoj Kumar Yadav; Rajesh Kumar Singh; D. L. Yadav
Abiotic stresses like drought, salinity, heat, cold, and osmotic pressure have adverse effects on a plant’s growth and development. The stress response in plants is mediated by several signaling pathways, mediated by the intervention of different phytohormones. The phytohormones that are linked to biotic and abiotic stresses are salicylic acid, jasmonic acid, auxin, abscisic acid, and gibberellin. The role of auxins, cytokinins, ethylene, gibberellins, brassinosteroids, and jasmonic acid has been studied in the context of developing abiotic stress-tolerant plants by metabolic engineering. The phytohormone abscisic acid (ABA) is a key hormone known to be involved in the regulation of abiotic stresses. ABA regulates osmolytes and detoxifies reactive oxygen species (ROS) as part of stress signaling pathways. During abiotic stress, signaling and plant response, phytohormones induce the expression of the transcription factors that regulate stress-responsive genes. Over the past few years, several transcription factors (TF) have been identified and are essential for regulating plant responses to these stresses. Activation of these TF can be ABA-dependent or independent in nature. In plants, several transcription factors like NAC, NAM, NF-Y, WRKY, bZIP, MYC, MYB, NAM, DREB, AP2, YABBY, Zinc finger, and their cis-acting elements regulate abiotic stresses in association with different plant hormones. This chapter highlights the ABA-dependent and independent transcription factors responsible for abiotic stress tolerance and their regulatory mechanisms. © 2026 selection and editorial matter, Kapil Gupta, Keshawanand Tripathi, Amit Joshi, and Dinesh Yadav.