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  1. Home
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Browsing by Author "Ratul Moni Ram"

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    PublicationBook Chapter
    A deeper insight into the symbiotic mechanism of Rhizobium spp. from the perspective of secondary metabolism
    (Springer Singapore, 2019) Prachi Singh; Rahul Singh Rajput; Ratul Moni Ram; Harikesh Bahadur Singh
    Rhizobia are group of organism, known globally for their nitrogen-fixing ability. In addition to nitrogen fixation, they significantly contribute to plant growth promotion and disease control. Rhizobial aptness for plant growth promotion and antagonism against a wide range of pathogens is due to its ability to produce a wide range of secondary metabolites such as HCN, siderophore, antibiotics, rhizobitoxin, lytic enzymes, IAA, phosphate solubilization and induced systemic resistance. Rhizobial inoculants, ascribed with multiple roles of nitrogen fixation, growth promotion and disease suppression, have strengthened crop productivity. This compilation urges the need to recognize and exploit the potential multifaceted secondary metabolites of rhizobia for biological control and growth promotion. © Springer Nature Singapore Pte Ltd. 2019.
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    PublicationArticle
    Biological management of Sclerotinia rot of bean through enhanced host defense responses triggered by Pseudomonas and Trichoderma species
    (Journal of Pure and Applied Microbiology, 2015) Ratul Moni Ram; Akansha Jain; Akanksha Singh; Harikesh Bahadur Singh
    Plant growth promoting microorganisms have been reported to induce systemic resistance in plants. In the present study, a consortium comprising of Trichoderma harzianum and Pseudomonas aeruginosa were evaluated for their efficacy to control Sclerotinia rot of bean. Upon infection of bean plants with the S. scIerotiorum resulted in substantial changes in enzymatic and non-enzymatic defense related compounds. The induction of phenylalanine ammonia-lyase (PAL), peroxidase (POx), polyphenoloxidase (PPO), superoxide dismutase (SOD), and the accumulation of proline and phenols in bean leaves were studied periodically at 2 d intervals after pathogen infection. Levels of PAL and phenols were induced most rapidly and increased significantly in all the treatments after pathogen challenge and reached maximum after 2 d and 4 d of pathogen challenge, respectively. Similarly, the activities of POx, PPO, SOD, along with proline content was also found to increase consistently and reached maximum at 6 d after pathogen challenge and their content was maximum in plants treated with microbial consortium. These results demonstrated that interaction of microorganism in rhizosphere could provide enhanced tolerance against pathogen through induction of host defenserelated compounds.
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    PublicationNote
    Discovering life on omics plane: The genius of Frederick Sanger
    (Indian Academy of Sciences, 2014) Chetan Keswani; Ratul Moni Ram; H.B. Singh
    [No abstract available]
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    PublicationBook Chapter
    Microbe-based novel biostimulants for sustainable crop production
    (Springer Singapore, 2019) Rahul Singh Rajput; Ratul Moni Ram; Anukool Vaishnav; Harikesh Bahadur Singh
    The emerging status and scope of microbial products for better plant growth and prevention of diseases have attracted attention of researchers, industrialists to promote this field and farmers to utilize them as microbial stimulants. The hazardous impact of chemical fungicides in our ecosystem can also be mitigated through these strategies. Owing to the multifarious applications of biostimulants, agriculturally important microorganisms (AIMs) have been incorporated in agricultural system as biofertilizers and biopesticides. AIMs employed multiple mechanisms including nutrient solubilization, production of siderophores, phytohormone, antimicrobial compounds and volatiles, ACC deaminase and exopolysaccharide to work as biostimulant for alleviation of abiotic and biotic stresses in plants. In the present chapter, a comprehensive study on microbial biostimulants has been emphasized to confer their growth promoting and stress alleviation activities in plants. This would surely facilitate in a profound perception about mechanism of the plant-microbe interaction. Once a better knowledge developed about the governing action mechanisms of the microbe-based biostimulants is made, it will be easy to target next generation of biostimulants which may have multitargeted approach. © Springer Nature Singapore Pte Ltd. 2019.
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    PublicationArticle
    Mixed PGPR consortium: an effective modulator of antioxidant network for management of collar rot in cauliflower
    (Taylor and Francis Ltd., 2019) Ratul Moni Ram; Ruchi Tripathi; Hareram Birla; Hagera Dilnashin; Surya Pratap Singh; Chetan Keswani
    Sclerotinia sclerotiorum is a cosmopolitan fungal pathogen causing stem and collar watery soft rot of cauliflower. Previous management of this disease with chemical pesticides caused hazardous results which lead to use of more eco-friendly microbial approaches. In the present study, consortia of Trichoderma harzianum TNHU27 and Pseudomonas aeruginosa PJHU15 were assessed for their ability in controlling Sclerotinia rot in cauliflower. The seedlings of cauliflower were challenged singly and in consortium with these two compatible microbes. The plants were evaluated upon challenge inoculation with S. sclerotiorum for changes in total phenolic content, the activity of defense and antioxidative enzymes. The microbial consortium comprising of T. harzianum and P. aeruginosa significantly enhanced the defense responses of the plant in comparison to pathogen challenged and unchallenged control. The study elucidates that plant beneficial microbes in the consortium may provide superior protection by induction of faster and enhanced defense responses in comparison to unchallenged and single microbe challenged plants under pathogen challenged conditions. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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    PublicationBook Chapter
    Mycorrhiza Based Approaches for Soil Remediation and Abiotic Stress Management
    (Springer Singapore, 2019) Ratul Moni Ram; Prakash Jyoti Kalita; Rahul Singh Rajput; H.B. Singh
    Anthropogenic activities have resulted into degradation of land and water bodies. Excessive mining activities lead to the contamination of nearby areas with heavy metals viz. Pb, As, Cd, Zn etc. All these elements are highly toxic to the plants when they are exposed at a higher concentration. Apart from these heavy metals even the excessive use of fertilizers, herbicides and unmetabolized antibiotics from livestock farming can also leaves considerable amount of toxic residues in the soil which hinder the overall growth of plants. In addition to this, abiotic stresses viz. drought stress, salt stress, osmotic stress and ozone stress etc. also limits the crop production. The level of their impact on the different crops across the globe varies depending upon the geographical location and unscrupulous human activities but altogether it results into a huge annual loss to the global crop productivity. Several approaches have been designed to figure out the stress right from artificial to biological. The biological approach includes the use of living organism for stress alleviation among which Arbuscular Mycorrhizal fungi (AMF) emerged as a potent tool for stress alleviation and phytoremediation. The term “phytoremediation” has got more and more attention over the past decade. Due to the multifaceted applications of AMF, it has been widely used as a xenobiotic tool. © Springer Nature Singapore Pte Ltd. 2019.
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