Browsing by Author "Ravi Thakur"

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Anti-cancer evaluation of quercetin embedded PLA nanoparticles synthesized by emulsified nanoprecipitation
(Elsevier B.V., 2015) Sanjeev K. Pandey; Dinesh K. Patel; Ravi Thakur; Durga P. Mishra; Pralay Maiti; Chandana Haldar
This study was carried out to synthesize quercetin (Qt) embedded poly(lactic acid) (PLA) nanoparticles (PLA-Qt) and to evaluate anti-cancer efficacy of PLA-Qt by using human breast cancer cells. PLA-Qt were synthesized by using novel emulsified nanoprecipitation technique with varying dimension of 32 ± 8 to 152 ± 9. nm of PLA-Qt with 62 ± 3% (w/w) entrapment efficiency by varying the concentration of polymer, emulsifier, drug and preparation temperature. The dimension of PLA-Qt was measured through transmission electron microscopy indicating larger particle size at higher concentration of PLA. The release rate of Qt from PLA-Qt was found to be more sustained for larger particle dimension (152 ± 9. nm) as compared to smaller particle dimension (32 ± 8. nm). Interaction between Qt and PLA was verified through spectroscopic and calorimetric methods. Delayed diffusion and stronger interaction in PLA-Qt caused the sustained delivery of Qt from the polymer matrix. In vitro cytotoxicity study indicate the killing of ~50% breast cancer cells in two days at 100. μg/ml of drug concentration while the ~40% destruction of cells require 5 days for PLA-Qt (46 ± 6. nm; 20. mg/ml of PLA). Thus our results propose anticancer efficacy of PLA-Qt nanoparticles in terms of its sustained release kinetics revealing novel vehicle for the treatment of cancer. © 2015 Elsevier B.V.
Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles
(Elsevier B.V., 2016) Sanjeev K. Pandey; Dinesh K. Patel; Akhilendra K. Maurya; Ravi Thakur; Durga P. Mishra; Manjula Vinayak; Chandana Haldar; Pralay Maiti
Tamoxifen (Tmx) embedded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-Tmx) is prepared to evaluate its better DNA cleavage potential, cytotoxicity using Dalton's lymphoma ascite (DLA) cells and MDA-MB231 breast cancer cells. PLGA-Tmx nanoparticles are prepared through emulsified nanoprecipitation technique with varying dimension of 17-30 nm by changing the concentrations of polymer, emulsifier and drug. Nanoparticles dimension are measured through electron and atomic force microscopy. Interactions between tamoxifen and PLGA are verified through spectroscopic and calorimetric methods. PLGA-Tmx shows excellent DNA cleavage potential as compared to pure Tmx raising better bioavailability. In vitro cytotoxicity studies indicate that PLGA-Tmx reduces DLA cells viability up to ~38% against ~15% in pure Tmx. Hoechst stain is used to detect apoptotic DLA cells through fluorescence imaging of nuclear fragmentation and condensation exhibiting significant increase of apoptosis (70%) in PLGA-Tmx vis-à-vis pure drug (58%). Enhanced DNA cleavage potential, nuclear fragmentation and condensation in apoptotic cells confirm greater bioavailability of PLGA-Tmx as compared to pure Tmx in terms of receptor mediated endocytosis. Hence, the sustained release kinetics of PLGA-Tmx nanoparticles shows much better anticancer efficacy through enhanced DNA cleavage potential and nuclear fragmentation and, thereby, reveal a novel vehicle for the treatment of cancer. © 2016 Elsevier B.V.
Corrigendum to “Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles” [Int. J. Biol. Macromol. 89 (2016) 99–110] (S0141813016303737) (10.1016/j.ijbiomac.2016.04.065))
(Elsevier B.V., 2018) Sanjeev K. Pandey; Dinesh K. Patel; Akhilendra K. Maurya; Ravi Thakur; Durga P. Mishra; Manjula Vinayak; Chandana Haldar; Pralay Maiti
The authors regretfully submit that there was human mistake and it can be rectified by adding a corrigendum in which the following be mentioned: [Figure presented] Fig. 1a incorrect one be immediately replaced by the Fig. 1a correct one. The human mistake was due to folders having electron micrograph of all experiment and during transfer of images from technical person to us. The authors would like to apologies for inconvenience caused. © 2018 Elsevier B.V.
Corrigendum to “Layered double hydroxides as effective carrier for anticancer drugs and tailoring of release rate through interlayer anions” [Journal of Controlled Release 224 (2106) 186–198] (Layered double hydroxides as effective carrier for anticancer drugs and tailoring of release rate through interlayer anions (2016) 224 (186–198), (S016836591630013X), (10.1016/j.jconrel.2016.01.016))
(Elsevier B.V., 2021) Sudipta Senapati; Ravi Thakur; Shiv Prakash Verma; Shivali Duggal; Durga Prasad Mishra; Parimal Das; T. Shripathi; Mohan Kumar; Dipak Rana; Pralay Maiti
The authors regret that the initial published version of this article an error in the assembly of Fig. 7b resulted in some image duplications. The corrected Fig. 7b includes the correct images of the experiment. This correction/omission doesn't alter any conclusion of the article as quantitative analysis of the experiment has been performed through MTT assay, presented in Fig. 7a. [Figure Presented] The figure legend remains the same. The corrections made in this corrigendum do not affect the original conclusions of the article. The author's apologies for any inconvenience caused. © 2016 Elsevier B.V.
Layered double hydroxides as effective carrier for anticancer drugs and tailoring of release rate through interlayer anions
(Elsevier B.V., 2016) Sudipta Senapati; Ravi Thakur; Shiv Prakash Verma; Shivali Duggal; Durga Prasad Mishra; Parimal Das; T. Shripathi; Mohan Kumar; Dipak Rana; Pralay Maiti
Hydrophobic anticancer drug, raloxifene hydrochloride (RH) is intercalated into a series of magnesium aluminum layered double hydroxides (LDHs) with various charge density anions through ion exchange technique for controlled drug delivery. The particle nature of the LDH in presence of drug is determined through electron microscopy and surface morphology. The release of drug from the RH intercalated LDHs was made very fast or sustained by altering the exchangeable anions followed by the modified Freundlich and parabolic diffusion models. The drug release rate is explained from the interactions between the drug and LDHs along with order-disorder structure of drug intercalated LDHs. Nitrate bound LDH exhibits greater interaction with drug and sustained drug delivery against the loosely interacted phosphate bound LDH-drug, which shows fast release. Cell viability through MTT assay suggests drug intercalated LDHs as better drug delivery vehicle for cancer cell line against poor bioavailability of the pure drug. In vivo study with mice indicates the differential tumor healing which becomes fast for greater drug release system but the body weight index clearly hints at damaged organ in the case of fast release system. Histopathological experiment confirms the damaged liver of the mice treated either with pure drug or phosphate bound LDH-drug, fast release system, vis-à-vis normal liver cell morphology for sluggish drug release system with steady healing rate of tumor. These observations clearly demonstrate that nitrate bound LDH nanoparticle is a potential drug delivery vehicle for anticancer drugs without any side effect. © 2016 Elsevier B.V. All rights reserved.