Browsing by Author "Ravindra Kumar"
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PublicationBook Chapter Ancient, mid-time, and recent history of seed pathology(Springer Singapore, 2020) Seweta Srivastava; Ravindra Kumar; Sumant Bindal; Vinit Pratap Singh; Meenakshi Rana; Jay Prakash Singh; Asha SinhaSeed is the basic unit of crop production. An optimum plant count depends on seed for their next progeny. Issues related to seed should be handled very carefully, since sowing of a poor-quality seed leads to undesirable loss in plant stand and hence crop yield. Focus on seed quality and seed health has been a point of serious consideration since time immemorial. Seed-related aspects are puzzling the researchers since long back. Well-developed science of seed pathology has been in existence for more than 100 years. Seed pathology includes studies on the mechanism of seed transmission, pathogenesis, epidemiology of seed-borne diseases, and control measures adopted against these diseases, ranging from crop management through chemical control to legislative precautions, as well as the technology of microbiological seed testing by covering the total range of seed-borne pathogens, viz., fungi, bacteria, viruses, nematodes, and physiological defects which may affect any member of the plant kingdom. The various aspects, viz., seed health, management of seed-borne diseases, seed treatments, and their detection and diagnosis techniques, were developed with the time. The systematic application of these techniques leads to the significant availability of healthy and disease-free quality seed. The institutional and technological developments in the science of seed pathology are being discussed in the present chapter. © Springer Nature Singapore Pte Ltd. 2020.PublicationArticle Antagonistic Behavior of Different Bioagents Against Dominant Seed Borne Fungi of Mungbean Seeds Under In Vitro Condition(Springer India, 2017) Saurabh Singh; Asha Sinha; S.M. Yadav; Bhupendra Kumar Singh; Harendra Singh; Shewta Srivastava; Ravindra KumarPulses are high value crops and generally greater attention is paid to protect them against diseases caused by microbial pathogens after harvest during storage. Use of fungicides may cause environmental hazards besides residual problems. Therefore Trichoderma viride, Trichoderma harzianum, Trichoderma virens, Pseudomonas fluorescence and Bacillus subtilis were evaluated in vitro against seed borne diseases of mungbean seeds. In vitro studies were carried out in both dual culture technique and Agar plate methods. The four dominant fungi of mungbean seeds viz, Aspergillus niger, Penicillium rubrum, Alternaria alternata and Fusarium moniliforme were used to assess their vulnerability to an array of bio-agents. Results showed that all bioagents significantly reduced the percent growth inhibition of all tested fungi. Among all the bioagents T. harzianum was found superior in controlling the seed borne mycoflora of mungbean seeds. Among the fungal pathogens, P. rubrum and A. niger were effectively suppressed by T. harzianum and P. fluorescence. Bacillus subtilis, T. virens and T. viride gave the promising results against all the tested fungi. Trichoderma harzianum showed highest inhibition of mycelium growth of the A. alternata followed by T.viride (8.00 %). Bacterial antagonist, B. subtilis showed intermediate effect against all tested fungi. Trichoderma virens and T. viride showed least effect against all tested seed borne fungi. © 2015, The National Academy of Sciences, India.PublicationArticle Association studies for yield and its component traits in tomato (Solanum lycopersicum L.)(2013) K. Srivastava; Kalyani Kumari; S.P. Singh; Ravindra KumarThe present investigations were conducted during winter 2010-11 at Vegetable Research Farm, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (U.P.), India. The experiment was laid out in Randomized Block Design with three replications. Correlations and path coefficient were studied in 52 exotic tomato (Solanum lycopersicum L.) genotypes for eight yield contributing characters to find out the inter relationship among the characters studied. Yield/plant was found highly significant and positively correlated with days to 50% flowering, days to 50% fruiting, plant height (cm), number of primary branches/plant, number of fruits/cluster, number of fruits/plant and average fruit weight (gm), which indicated that yield could be increased by improving a traits. In order to obtain a clear picture of the inter relationship between yield per plant and its components, direct and indirect effects were measured using path coefficient analysis. The path coefficient analysis splits total correlation coefficient of different characters in to direct effect and indirect effect in equal to genotypic correlation. The data revealed that average fruit weight (1.0218) showed the highest positive direct effect on yield/plant followed by number of fruits/plant (0.7286), day to 50% flowering, number of primary branches/plant (0.1101) and number of fruits/cluster (-0.3707) and plant height (-0.0617) showed negative direct effects. The characters showed high direct effect on yield/plant indicated that direct selection for these traits might be effective and there is a possibility of improving yield/plant through selection based on these characters. Residual effect was considerably low (0.183) which indicated that characters included in this study explained almost all variability towards yield.PublicationBook Chapter Bioremediation Potential of Rhizobacteria associated with Plants Under Abiotic Metal Stress(wiley, 2021) Shrvan Kumar; Saroj Belbase; Asha Sinha; Mukesh Kumar Singh; Brajesh Kumar Mishra; Ravindra KumarBiological remediation is considered as the most effective method of toxic metal removal as these are natural, environmentally friendly, low-cost, and high societal acceptance technologies. One such technology is the use of plant growth promoting microbes for bioremediation of heavy metal polluted soil and is quite important in the context of the global climate change and excessive fertilizer use in agricultural soils. This is feasible because microorganisms have developed many resistance mechanisms for survival in the presence of toxic heavy metals in their environment. Microbes also enhance bioavailability of metals from soil by chelation, acidification, and precipitation as, for example, organic acids released by microbes and plant roots lower the soil pH and help in the sequestration of metal ions. Plant growth promoting rhizobacteria (PGPR) are the microorganism basically present in the rhizosphere, which includes the bacterial species Alcaligenes, Azospirillum, Arthrobacter, Acinetobacter, Azoarcus, Bradyrhizobium, Bacillus, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Pseudomonas, Pantoea, Paenibacillus, Rhizobium Azorhizobium, Bradyrhizobium, Allorhizobium, Sinorhizobium, Methylobacterium, Frankia, and Mesorhizobium. Genetically transformed bacteria possess one or more genes to increase the remediation of heavy metals. Against this background, genes for metal chelators, metal homeostasis, transporters, biodegradative enzymes, metal uptake regulators, and biotic and abiotic stress tolerance are important candidates for making recombinant bacteria. The heavy metals Hg, Cr, As, Zn, Cd, Pb, Ur, Se, Ag, Au, and Ni are hazardous heavy metals. Among heavy metals, four heavy metals As, Pb, Cd, Zn, and Hg are considered as the most toxic metals by the US Agency for Toxic Substances and Disease Registry, based on their toxicity, frequency of occurrence, and most importantly, their exposure potential to flora and fauna. The effectiveness of bioremediation depends on several factors such as the nature of the organisms utilized, the prevailing environmental factors at the contaminated site, as well as the degree of the pollutants in that environment. © 2021 John Wiley & Sons Ltd. All rights reserved.PublicationArticle Biotechnological role of fungal microbes in sustainable agriculture(Plant Archives, 2019) Seweta Srivastava; Sumant Bindal; Meenakshi Rana; Ravindra Kumar; Sangeeta Yadav; Jay Prakash Singh; Asha SinhaOver the past several decades, advances in biotechnology have been used as a tool to increase food production. Specifically, advances in genetic engineering have made possible the manipulation of crops to increase yield, guaranteeing food supplies for the increasing world population. The broad application of microbes in sustainable agriculture is due to the genetic dependency of plants on the beneficial functions provided by symbiotic cohabitants. Therefore, microbial biotechnology and its applications in sustainable development of agriculture and environmental health are getting better attention. Finally, a brief highlight has been given on the biotechnological role of mycobionts to sustain the agriculture sector. © 2019 Plant Archives. All rights reserved.PublicationArticle Chromosomal distribution of constitutive heterochromatin in eight species of mahseers (Family: Cyprinidae) from India(2013) Indra Mani; Ravindra Kumar; Mamta Singh; B. Kushwaha; N.S. Nagpure; P.K. Srivastava; W.S. LakraThe chromosomal distributions of constitutive heterochromatin were analyzed in 8 species of mahseer, namely, Tor chelynoides (McClelland), T. khudree (Sykes), T. mosal mahanadicus (David), T. mussullah (Sykes), T. putitora (Hamilton), T. progeneius (McClelland), T. tor (Hamilton) and Neolissochilus hexagonolepis (McClelland) using C-banding technique. The constitutive heterochromatin bands were observed on several pairs of chromosomes with maximum numbers in T. progeneius (11 pairs), followed by T. putitora (7 pairs), T. mosal mahanadicus (4), T. khudree (3), T. mussullah (3), N. hexagonolepis (3), T. chelynoides (3) and T. tor (2). In all species, the C-bands were observed mainly on p arm with few on centromeric region of chromosomes. This seems to be the characteristic feature for characterization of these species. The presence of similar chromosome numbers (2n=100) with diverse karyotypes in the mahseer species suggests evolution among the species through pericentric inversions and/or heterochromatin processes. The number and location of the heterochromatic bands were found species-specific and thus useful for the cytotaxonomy of these species. The findings of the present study can also help to understand the evolutionary relationships among the mahseer species.PublicationBook Chapter Diagnosis and Detection of Major Airborne Fungal Phytopathogens(Springer Science+Business Media, 2025) Ravindra Kumar; Seweta Srivastava; Aishwarya Singh Rathore; R. C. Tiwari; Shyam Saran Vaish; Shiv Shankar Patel; S. Dasaratha Kumar; Sunil Kashyap; Udai B. Singh; Gyanendra Pratap SinghFood safety and security are at risk because agricultural crops are infected by a number of pathogens, including bacteria, fungus, viruses, viroids, and plant viruses. The diminutions in foods caused by these phytopathogenic agents have been persistent concerns related to agriculture for generations all throughout the world. Airborne fungal phytopathogens create significant threats to agricultural ecosystems, causing sizeable economic losses and jeopardizing food security worldwide. When developing management methods, the most crucial factors are thought to be the prompt detection and precise identification of the causative agents linked to crop diseases. The methods and technologies now used in the identification and detection of the main airborne fungal phytopathogens are thoroughly reviewed in this chapter. Firstly, traditional diagnostic techniques such as microscopy, culture-based methods, and air samplers are discussed, highlighting their advantages and limitations. Additionally, molecular techniques, including polymerase chain reaction (PCR)-based assays, loop-mediated isothermal amplification (LAMP), etc., are examined for their sensitivity, specificity, and applicability in detecting airborne fungal pathogens. Overall, this chapter aims to provide valuable insights for researchers, practitioners, and policymakers involved in the management and surveillance of airborne fungal phytopathogens, fostering advancements in disease control strategies and safeguarding global crop production. © 2025 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.PublicationArticle Effect of high density planting systems on physiological and biochemical status of rejuvenated mango plants of cv. Amrapali(Indian Academy of Horticultural Sciences, 2017) Amit Raj; V.B. Patel; Ravindra Kumar; Kalyan Barman; R.B. Verma; Sashikant; S.K. PathakMango trees grown under high density planting systems show a gradual decline in yield after 11-12 years due to overcrowding of canopies. To find out the effect of high density planting systems on physio-biochemical parameters of mango, an experiment was conducted on an old senile, rejuvenated high density cv. Amrapali orchard over two consecutive years. High density planting systems showed significant effect on physiological, biochemical and nutritional parameters of plants. Among the different planting systems, plants under cluster planting system recorded the highest leaf area (156.68 cm2), transpiration rate (3.77 m mol-1 m-2 s-1); and N (1.28%) P (0.20 %) and K (0.54%) contents. The maximum leaf relative water content (87.63%) was noted in plants under square planting system, while, the photosynthetic rate (8.36 µmol CO2 m-2 s-1) was highest in hedge-row planting system. The stomatal conductance (0.17 µmol CO2 m-2 s-1) was the highest in plants under double-hedge row planting system. The highest internal carbon concentration was recorded in paired planting system, while the maximum chlorophyll ‘a’ (1.50 mg g-1), chlorophyll ‘b’ (0.65 mg mg g-1) and total chlorophyll (2.02 mg g-1) contents were found in plants under square planting system. The highest total phenolics (53.09 mg g-1) content was recorded under paired planting system. The micronutrient contents in plants, viz. Cu, Zn, Fe and Mn also differed among different planting systems. © 2017, Horticulture Society of India. All rights reserved.PublicationArticle Effect of organic amendments on soil mycoflora(2010) Ravindra Kumar; Seweta Srivastava; Manisha Srivastava; Asha SinhaIn present study, the influences of different organic soil amendments on soil fungi was studied. Soil organisms carry a wide range of processor that are important for soil health and partially in both natural and managed agricultural scales. The total number of organisms, the diversity of species and activity of soil biota will fluctuate as soil environment changes. Three types of soil amendments and fertilizers viz., urea, FYM and vermicompost were used to amend the cultivated agricultural soil. The fungi were isolated from soil by using dilution plate technique and soil plate method. The dynamics of soil fungi were observed qualitatively as well as quantitatively. The maximum number of fungi was recorded when soil amended with FYM (40.6xl04 g-1), urea (38.8xl04 g-1) of dry soil at different concentration 2.0, 1.5 and 2.0%, respectively. In control where soil was not amended with any organic amendment, the number of fungi was 13.0xl04 to 16.8x104 g-1 14.4xl04 to 16.8x104 g-1 13.8xl04 to 16.8xl04 g-1 in urea, FYM and Vermicompost, respectively. A total 25 fungi were observed during the experimental period. Eighteen were observed when soil amended with urea, twenty-two observed when soil amended with FYM and 20 when soil amended with vermicompost. In control soil only fifteen fungi were recorded. The result showed that the number of fungi was increased in amended soil. Qualitatively, the fungi Rhizopus stolomfer, Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Penicillium citrinum, Alternaria alternata and Curvularia lunata, White Sterile Mycelium and Black Sterile Mycelium were observed. © 2010 Academic Journals Inc.PublicationArticle Effect of seed-borne mycoflora on protein and amino acid content of Jatropha curcas L. Seeds during storage(2013) Seweta Srivastava; Manisha Srivastava; Ravindra Kumar; Asha SinhaJatropha curcas is a non-edible oil crop predominately used to produce bio-diesel. J. curcas seed meal (with 1 -2 % residual oil) has 58 -64 % crude protein (90 % of which is present in the form of true protein) and levels of essential amino acids except lysine are higher which were constantly effected by the seed-borne mycoflora during storage. The objective of this study was to estimate the change in protein and amino acid content of Jatropha seeds after deterioration. For protein and amino acid estimations fresh, stored as well as infested Jatropha seeds were used. Whole seed and kernels were infested with six fungi isolated from Jatropha curcas seeds during storage viz. Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Fusarium chlamydosporum and Penicillium glabrum separately. Four different concentrations of seed samples viz. 50μl, 100μl, 150μl and 200μl were taken for the protein and amino acid estimation of stored as well as infested Jatropha seeds. Protein content of two years stored Jatropha seeds at 200μl concentration is much less as compared to fresh seeds. Minimum protein content was found in Fusarium chlamydosporum infested Jatropha seeds i.e., 10μg/ml which is very less as compared to fresh seeds i.e., 110.67μg/ ml at 50μl concentration respectively. Protein profiling by SDS-PAGE revealed that the maximum number of bands was shown by fresh Jatropha seeds and kernels. The presence or absence of bands in protein profiling might be responsible for presence or absence of protein of that size. Surprisingly, amino acids of infested seeds were increased due fungal infection and maximum amino acids content was found in Aspergillus flavus infested kernels at 200μl concentration followed by Fusarium chlamydosporum and Aspergillus flavus infested Jatropha kernels.PublicationArticle Efficacy of vinblastine and prednisone in multicentric reticulohistiocytosis with onset in infancy(American Academy of Pediatrics, 2016) Vijay Kumar Jha; Ravindra Kumar; Abhijeet Kunwar; Ankur Singh; Mahendra Kumar; Mohan Kumar; Rajniti PrasadMulticentric reticulohistiocytosis (MRH) is a rare histiocytic proliferative disorder of uncertain etiology, characterized by mucocutaneous papulonodular lesions and progressive, symmetric erosive arthritis. MRH can coexist with various autoimmune disorders, tuberculosis, and malignancy. It usually occurs in the elderly and is very rare in children. This is probably the first case in which disease manifestation appeared in infancy in the form of skin lesions. The patient had recurrent ulceration of cutaneous lesions, which is unusual in MRH. Early diagnosis and aggressive treatment are essential to prevent progressive irreversible course and development of arthritis mutilans. Various drugs, such as steroids, nonsteroidal anti-inflammatory drugs, immunosuppressants, interleukin inhibitors, and tumor necrosis factor-α antagonist, have been tried with variable responses. The present case responded well to vinblastine and steroid, which have not been reported previously. Here, we document a case of MRH with early onset in infancy along with role of vinblastine and prednisone in its treatment. Copyright © 2016 by the American Academy of Pediatrics.PublicationArticle Evaluation of biocontrol potential of some fungal decomposers of Sesbania aculeata L. Green manure against some soil-borne plant pathogens(Triveni Enterprises, 2017) Ravindra Kumar; Asha Sinha; Seweta Srivastava; Gaurav MahajanAim: Soil borne phytopathogens are one of the major concern of today's agricultural system. In the present study, dominant fungal decomposers were selected and their potential as biological control agents was evaluated against some soil borne plantpathogens. Methodology: Effect of green manure amendment on the sclerotia viability of three soil-borne plant pathogens viz., Sclerotium rolfsii, Rhizoctonia solani and Sclerotinia sclerotiorum, effect of fungal decomposers on soil-borne phytopathogens in dual culture, effect of volatile and non-volatile metabolites of dominant fungal decomposers on the radial growth and sclerotia production of test pathogens were evaluated. Results: Green manure amendment resulted in reduced in sclerotia of S. rolfsii, R. solani and S. sclerotiorum by 40, 36 and 36.50%, respectively. In dual culture, the maximum growth inhibition of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 49.95, 47.62 and 57.83%, respectively The maximum inhibition of S. rolfsii and S. sclerotiorum caused by the volatile metabolites produced by Trichoderma harzianum were 56.64 and 43.95%, whereas the maximum inhibition of R. solani was caused by volatile metabolites of Penicillium citrinum (44.96%). The maximum inhibition of S. rolfsii, R. solani and S. sclerotiorum through non-volatile metabolites of Trichoderma harzianum were 51.04,57.30 and 49.10%, respectively. The maximum reduction in sclerotia of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 86.44, 88.54 and 88.20% respectively, under dual culture after 21 days of incubation. The maximum reduction in sclerotia of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 95.42, 93.60 and 91.32%, respectively, under the effect of volatile metabolites after 21 days of incubation. The maximum reduction in sclerotia of S. rolfsii, R. solani and S. sclerotiorum with Trichoderma harzianum were 87.46, 79.74 and 85.10% respectively, under the effect of non-volatile metabolites after 21 days of incubation. Interpretation: All eight fungal decomposers effectively contribute in controlling the soil borne phytopathogens. Overall T harzianum, Aspergillus niger and Penicillium citrinum proved as potential bio-control agents against all soil borne plant pathogens viz., S. rolfsii, R. solani and S. sclerotiorum. © Triveni Enterprises, Lucknow (India).PublicationArticle Evaluation of pigeonpea genotypes for their resistance against pod fly, Melanagromyza obtusa (Malloch) under natural conditions(Malhotra Publishing House, 2018) Sunil Verma; R.P. Maurya; Gouri Shankar Giri; Ravindra KumarAn experiment was conducted to screen thirty one genotypes of pigeonpea with three checks viz., PUSA 992, PA 291 & UPAS 120 for their resistance/tolerance to M. obtusa under natural infestation. None of the genotypes was found resistant, but marked differences were noticed among different genotypes. On the basis of larval population of M. obtusa and pod damage twelve genotypes were found to be least susceptible, while nine genotypes were moderately susceptible and ten genotypes were highly susceptible on a rating scale of 1-9. © 2018, Malhotra Publishing House.PublicationArticle Floral and flushing pattern of baramasi, regular and biennial bearing cultivars of mango in Eastern India(Indian Academy of Horticultural Sciences, 2019) D.K. Tiwari; V.B. Patel; Kalyan Barman; Ravindra Kumar; R.B. Verma; S.N. Singh; B.B. MishraFlowering in mango is a very complex phenomenon. The potentiality to form flower buds depends on the florious condition of the tree that depends up on many factors like genotype, climatic factors, physiological behaviour, agro-techniques, pests and disease management etc. The present study was carried out on flowering and flushing behaviour of Baramasi, regular and biennial bearing cultivars of mango at Bihar (Eastern Indian) condition. Results indicated that Baramasi had more than four times panicle and flush emergence in a year. Time of panicle emergence was the earliest in cv. Alphonso among all the cultivars. The maximum number of panicles per branch was recorded in Langra (725.21) during its ‘on’ year. However, it was the minimum in cv. Baramasi. The maximum number of flowers per panicle was recorded in cv. Langra (1215 and 1132) during both the years of study. The longest panicle was measured in cv. Alphonso (31.72-33.99 cm) while, the shortest panicle was observed in cv. Baramasi (21.30-29.02 cm). Cultivar Alphonso took more time (35.6 and 33.2 days) for bud break to full bloom whereas; Amrapali (23.80 and 22.14 days) took fewer periods during both the years. The shortest duration of flowering was also noticed (17 days) in cv. Amrapali. Cultivar Langra had the maximum number of hermaphrodite flowers (65.0 and 66.2) as well as flowering intensity (4448 and 4143 flowers/ft2) however, it was the minimum in cv. Baramasi (23.8 and 24.4%and 2006.4 and 3235.6 flowers/ft2) during both the consecutive years. Higher incidence of malformation was found in cv. Amrapali but at the same conditions cv. Langra did not show any incidence of malformation. Variation in floral and flushing behaviour among the cultivars might be due to variation in climatic conditions and individual genetic characteristics. © 2019, Horticulture Society of India. All rights reserved.PublicationArticle Gene effects and heritability for yield traits in tomato (Solanum Lycopersicum L.)(Bangladesh Botanical Society, 2021) Ravindra Kumar; K. SrivastavaGeneration mean analysis is a key determinant in designing the appropriate breeding approaches for implementing new desirable character/s in crop varieties and also in studying inheritance of quantitative traits. Parents, F1, F2 and backcrosses exhibited significant digenic interactions in the majority of cross combinations in tomato. Cross combinations and characters indicated the adequacy of simple additive dominance for 50% flowering (CO3 х Floradade, Pant T3 х Azad T5), branches/plant and plant height (CO3 х Floradade, Pant T3 х Kashi Sharad), and fruit/cluster (CO3 х Floradade) indicating absence of non-allelic interactions. Duplicate type of epistasis occurred for 50% flowering (CO3 х Floradade, Kashi Amrit х Kashi Sharad), branches/plant (CO3 х Floradade, Kashi Amrit х Kashi Sharad, Pant T3 х Kashi Sharad), plant height (CO3 х Floradade, Pant T3 х Kashi Sharad), fruit/plant (CO3 х Floradade, Pant T3 х Azad T5, Kashi Amrit х Kashi Sharad, Pant T3 х Kashi Sharad), fruit weight (CO3 х Floradade, Pant T3 х Azad T5), fruit/cluster (Kashi Amrit х Kashi Sharad, Pant T3 х Kashi Sharad), yield/plant (CO3 х Floradade, Punjab Upma х Azad T5, Pant T3 х Kashi Sharad) indicating selection strength should be lenient in the earlier and rapid in later generations because advancement should be through selection. Characters governed by additive components were predominant in fruit/plant and plant height, indicating direct selection for improvement of these traits. © 2021 Bangladesh Botanical Society. All rights reserved.PublicationArticle Genetic parameters and correlation study for yield and quality traits in tomato (Solanum lycopersicum L.)(2013) Vinod Kumar; R. Nandan; K. Srivastava; S.K. Sharma; Ravindra Kumar; Anuj KumarThe present investigation was conducted at Vegetable Research Farm, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (U.P.), India during Rabi 2008-09. The genetic parameters and correlation were studied for yield and quality traits in tomato. The experimental material comprised of thirteen genotypes (10 lines + 3 testers) and their thirty crosses alongwith two checks of tomato and the experiment was laid out in Randomized Block Design with three replications. The maximum genotypic and phenotypic variation was (168.30 and 169.95 cm.) for plant height and minimum for fruit shape index (0.02 and 0.02), respectively. Analysis of coefficient of variation revealed that magnitude of phenotypic coefficient was higher than genotypic coefficient of variation for all the characters except primary branches per plant under study. The magnitude of genotypic and phenotypic coefficient of variation was higher for number of fruits per cluster (18.04 and 18.32), lycopene content (22.07 and 22.46), respectively. High values of heritability (broad sense) for plant height (99%) and fruit per cluster, total soluble solids and lycopene (97%) and high genetic advance were observed for plant height and average fruit weight (26.59 and 14.88%), respectively. A positive and significance association of yield per plant with all the traits both at genotypic and phenotypic levels.PublicationArticle Gerbode defect of congenital variety in an infant: A case report(Journal of Clinical and Diagnostic Research, 2016) Ankur Singh; Ravindra Kumar; Abhishek Abhinay; Rajniti Prasad; Om Prakash MishraGerbode defect is a rare communication from left ventricle to right atrium. It is of two types: congenital versus acquired OR Direct (type I) versus Indirect (type II). Acquired forms are more common and increasingly reported than congenital. We report a second Indian case of such a rare defect and highlight the salient points of all such previously reported cases to make aware the clinicians and paediatricians of need of early diagnosis and timely surgery/ referral for successful outcome. © 2016, Journal of Clinical and Diagnostic Research. All rights reserved.PublicationArticle Heterosis analysis in tomato (Solanum lycopersicum L.) for Lycopene, TSS, titrable acidity and Ascorbic acid(Indian Society of Plant Breeders, 2019) Ravindra Kumar; K. Srivastava; Vinod Kumar; S.K. Saroj; S.K. Sharma; R.K. SinghHeterosis were estimated using ten lines and three testers and their thirty F1 combinations, crossed in line x tester fashion which were evaluated for four seasons for important quality traits viz., lycopene, ascorbic acid, titrable acidity (TA), total soluble solids (TSS) and pericarp thickness. Some of the parents having good potentiality for generating superior F1 combinations for most of the quality characters under study have been identified. The analysis of variance (ANOVA) indicated significantly higher amount of differences among genotypes for all the five characters studied. In this study, among crosses, the cross NDTVR60 x Floradade exhibited positive desirable heterosis over best parent for lycopene (60.22%) and Selection7 x Floradade (25.12%) for ascorbic acid. The cross NDTVR60 x Floradade showed the highest positive heterosis over best parent for titrable acidity (31.37%) whereas cross CO3 x Azad T5 (30.65%) exhibited the significantly highest positive heterosis over best parent for TSS and cross combination DT2 x Azad T5 (56.82%) exhibited positive heterobeltiosis for pericarp thickness. © Indian Society of Plant Breeders.PublicationArticle Heterosis for quality attributes in tomato (Lycopersicon esculentum Mill.)(2013) Ravindra Kumar; K. Srivastava; R.K. Singh; Vinod KumarIn present study 43 genotypes (13 parental lines and 30 F1 hybrids) were evaluated during 2010 and 2011 in order to estimate the extent of heterosis and quality trait like ascorbic acid (vitamin C), titratable acidity, lycopene content and total soluble solids (TSS). Significant differences among genotypes were obtained for all four traits. Positive significant heterosis was found for ascorbic acid (32.52% and 23.07% over better parent as well as 30.81% and 15.98% over standard check BT-12), titratable acidity (25.49% and 26.42% over better parent as well as 14.04% and 16.48% over standard check), lycopene content (63.99% and 71.25% over the better parent as well as 77.26% and 62.27 % over standard check) and TSS (27.32% and 34.86% over better parent as well as 17.25% and 13.61% over standard check) over both the years, respectively.PublicationArticle Heterosis for yield and yield components in Tomato (Lycopersicon esculentum Mill)(Indian Society of Plant Breeders, 2012) Ravindra Kumar; K. Srivastava; J. Somappa; Sunil Kumar; R.K. SinghA trial comprising 15 hybrids and 8 parental lines was in conducted a randomized block design with three replications during winter 2010-11 at Vegetable Research Farm, Banaras Hindu University, Varanasi, India. Heterosis was estimated in fifteen single experimental cross hybrids, obtained by five parental lines namely H-24, DT-2, CO-3, Punjab Upma, Pant T-3 and three testers of tomato viz. Floradade, Kashi Sharad, Azad T-5 for yield and yield related traits; plant height, days to 50% flowering, number of fruits per plant, average fruit weight, fruit diameter, number of fruits per cluster and total yield per plant. Significant differences among genotypes were observed for all the traits. Positive and highly significant heterosis was found for number of fruits per plant 25.27%, 25.13% and 21.13% over better parent and 29.95%, 25.27% and 24.46% over standard parent and for total yield per plant 32.06%, 18.34%, 13.36% and 11.27% over better parent and 31.83%, 31.14%, 30.10% and 25.26% over standard check 'Azad T-5'. The hybrid also showed significantly high percentage of positive heterosis over better and standard parent for number of fruits per cluster, average fruit weight and the hybrids showed negative heterosis for plant height and day to 50% flowering which are desirable characters. © Indian Society of Plant Breeders.
