Browsing by Author "Reena Mukherjee"

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Evaluation of canine bone marrow-derived mesenchymal stem cells for experimental full-thickness cutaneous wounds in a diabetic rat model
(Taylor and Francis Ltd., 2021) Deepika Bist; A.M. Pawde; Amarpal; Prakash Kinjavdekar; Reena Mukherjee; K.P. Singh; Med Ram Verma; Khan Sharun; Amit Kumar; Pawan K. Dubey; Divya Mohan; Amit Verma; G. Taru Sharma
Background: The wound healing potential of canine bone marrow-derived mesenchymal stem cells (BMSCs) was evaluated in the excisional wound of streptozotocin-induced diabetic rats. Research design and methods: Xenogenic BMSCs were collected aseptically from the iliac crest of healthy canine donors under general anesthesia. Full-thickness experimental wounds (20 × 20 mm2) on the dorsum of forty-eight adult healthy Wistar white rats. The wounds were assigned randomly to three treatment groups: PBS (Group A) or BMSCs (Group B) injected into the wound margins on days 0, 7, and 14 or BMSCs (Group C) injected into the wound margins on days 7, 14, and 21 post-wounding. The degree of wound healing was evaluated based on macroscopical, hemato-biochemical, histopathological, and histochemical parameters. Results: The results indicated granulation tissue formation with reduced exudation and peripheral swelling in the treatment groups compared to the control group A. Similarly, the degree of wound contraction was significantly higher in groups B and C animals than group A on days 14 and 21 post-wounding. The transplantation of BMSCs resulted in early drying of wounds, granulation tissue appearance, and enhanced cosmetic appearance. Conclusion: The histopathological, histochemical, and gross findings suggested the therapeutic potential of xenogeneic mesenchymal stem cell therapy in managing diabetic wounds. Abbreviations: BMSCs-bone marrow-derived mesenchymal stem cells, PBS-phosphate-buffered saline, MSCs-mesenchymal stem cells, FBS-fetal bovine serum, ECM-extracellular matrix. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Nutritional secondary hyperparathyroidism–induced facial osteodystrophy in a Labrador puppy
(Springer Science and Business Media Deutschland GmbH, 2021) Deeksha Bharti; Y. Ajith; E. Madhesh; Naveen Kumar Verma; Mamta Singh; E. Kalaiselvan; Raja Raghuvaran; Srishti Soni; Kruti Debnath Mandal; Reena Mukherjee; Umesh Dimri
Parathormone is a key polypeptide hormone controlling bone remodeling by influencing calcium, phosphorus, and vitamin D homeostasis in mammalian body. This report describes unusual presentation of osteodystrophy causing facial hyperostosis due to nutritional secondary hyperparathyroidism in a puppy. A female Labrador Retriever dog aged 5 months was presented with progressive bilaterally symmetrical swelling on facial region, hypersalivation, and reduced growth rate. Excessive meat intake in daily ration since weaning was reported. Clinical examination revealed dyspnoea, open mouth breathing, epiphora, excessive nasal secretions, tachycardia, pale mucous membranes, debility, loosely attached teeth, and pigmented oral mucosa of the upper jaw. Microcytic anemia, lymphocytosis, eosinophilia, monocytopenia, hypoproteinemia with hypoglobulinemia, hyperalbuminemia, hypocalcemia, hyperphosphatemia, and decreased Ca:P ratio were evident in the hemato-biochemical study. Lateral radiographic view of the head revealed hyperostosis of the facial bones with irregularly arranged teeth on the upper jaw. Nutritional secondary hyperparathyroidism–induced facial osteodystrophy was diagnosed in the animal based on the history and other supporting clinical evidence. The animal was treated with a protocol extending for 4 weeks using calcium, calcitriol, essential minerals, vitamins, and steroids. The animal showed considerable improvement on day 14 and regained normal facial architecture by day 28 of the therapy. © 2021, The Author(s), under exclusive licence to Springer-Verlag London Ltd. part of Springer Nature.