Browsing by Author "Renu Singh"

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Allelic sequence variation in the Sub1A, Sub1B and Sub1C genes among diverse rice cultivars and its association with submergence tolerance
(Nature Research, 2020) Anuradha Singh; Yashi Singh; Ajay K. Mahato; Pawan K. Jayaswal; Sangeeta Singh; Renu Singh; Neera Yadav; A.K. Singh; P.K. Singh; Rakesh Singh; Rajesh Kumar; Endang M. Septiningsih; H.S. Balyan; Nagendra K. Singh; Vandna Rai
Erratic rainfall leading to flash flooding causes huge yield losses in lowland rice. The traditional varieties and landraces of rice possess variable levels of tolerance to submergence stress, but gene discovery and utilization of these resources has been limited to the Sub1A-1 allele from variety FR13A. Therefore, we analysed the allelic sequence variation in three Sub1 genes in a panel of 179 rice genotypes and its association with submergence tolerance. Population structure and diversity analysis based on a 36-plex genome wide genic-SNP assay grouped these genotypes into two major categories representing Indica and Japonica cultivar groups with further sub-groupings into Indica, Aus, Deepwater and Aromatic-Japonica cultivars. Targetted re-sequencing of the Sub1A, Sub1B and Sub1C genes identfied 7, 7 and 38 SNPs making 8, 9 and 67 SNP haplotypes, respectively. Haplotype networks and phylogenic analysis revealed evolution of Sub1B and Sub1A genes by tandem duplication and divergence of the ancestral Sub1C gene in that order. The alleles of Sub1 genes in tolerant reference variety FR13A seem to have evolved most recently. However, no consistent association could be found between the Sub1 allelic variation and submergence tolerance probably due to low minor allele frequencies and presence of exceptions to the known Sub1A-1 association in the genotype panel. We identified 18 cultivars with non-Sub1A-1 source of submergence tolerance which after further mapping and validation in bi-parental populations will be useful for development of superior flood tolerant rice cultivars. © 2020, The Author(s).
Co-Implementation of Tillage, Precision Nitrogen, and Water Management Enhances Water Productivity, Economic Returns, and Energy-Use Efficiency of Direct-Seeded Rice
(MDPI, 2022) Vijay Pratap; Anchal Dass; Shiva Dhar; Subhash Babu; Vinod Kumar Singh; Raj Singh; Prameela Krishnan; Susama Sudhishri; Arti Bhatia; Sarvendra Kumar; Anil Kumar Choudhary; Renu Singh; Pramod Kumar; Susheel Kumar Sarkar; Sunil Kumar Verma; Kavita Kumari; Aye Aye San
The sustainability of conventional rice (Oryza sativa L.) production systems is often questioned due to the over-mining of groundwater and environmental degradation. This has led to the development of cost-effective, resource-efficient, and environmentally clean rice production systems by optimizing water and nitrogen (N) use. Hence, a 2-year field study (2019 and 2020) was conducted at the ICAR–Indian Agricultural Research Institute, New Delhi, to assess the effect of precision N and water management strategies on growth, land, and water productivity, as well as energy-use efficiency in scented direct-seeded rice (DSR). Two crop establishment methods, conventional-till DSR (CT-DSR) and zero-till DSR (ZT-DSR) along with three irrigation scenarios (assured irrigation (irrigation after 72 h of the drying of surface water), irrigation at 20% depletion of available soil moisture (DASM), and 40% DASM+Si (80 kg ha−1)) were assigned to the main plots; three N management options, a 100% recommended dose of N (RDN): 150 kg ha−1; Nutrient Expert® (NE®)+leaf color chart (LCC) and NE®+soil plant analysis development (SPAD) meter-based N management were allocated to sub-plots in a three-time replicated split-plot design. The CT-DSR produced 1.4, 11.8, and 89.4, and 2.4, 18.8, and 152.8% more grain yields, net returns, and net energy in 2019 and 2020, respectively, over ZT-DSR. However, ZT-DSR recorded 8.3 and 10.7% higher water productivity (WP) than CT-DSR. Assured irrigation resulted in 10.6, 16.1 16.9, and 8.1 and 12.3, 21.8 20.6, and 6.7% higher grain yields, net returns, net energy, and WP in 2019 and 2020, respectively, over irrigation at 20% DASM. Further, NE®+SPAD meter-based N management saved 27.1% N and recorded 9.6, 18.3, 16.8, and 8.3, and 8.8, 21.7, 19.9, and 10.7% greater grain yields, net returns, net energy, and WP over RDN in 2019 and 2020, respectively. Thus, the study suggested that the NE®+SPAD-based N application is beneficial over RDN for productivity, resource-use efficiency, and N-saving (~32 kg ha−1) both in CA-based and conventionally cultivated DSR. This study also suggests irrigating DSR after 72 h of the drying of surface water; however, under obviously limited water supplies, irrigation can be delayed until 20% DASM, thus saving two irrigations, which can be diverted to additional DSR areas. © 2022 by the authors.
Diagnostic Methods of Parasitic Diseases of Poultry
(Bentham Science Publishers, 2025) S. Kumar; Pradeep Kumar; R. L. Rakesh; Alok Kumar Singh; Vivek Agarwal; Krishnendu Kundu; Renu Singh
The use of diagnostic methods for the diagnosis of parasitic diseases in poultry has been almost constant over the past few decades. Since the introduction of PCR, few major advances have been adopted in clinical diagnostic tests. Many diagnostic tests that form the backbone of the “modern” microbiology laboratories rely on very old and labour-intensive technologies such as microscopy for the diagnosis of parasites including helminths, protozoans, arthropods, and haemoprotozoans. Urgent needs include more rapid tests without compromising the sensitivity, value-added tests, and point-of-care tests for both high- and low-resource settings. In recent years, research has been focused on alternative methods to improve the diagnosis of parasitic diseases. These include molecular technique-based approaches, immunoassays and proteomics using mass spectrometry platforms technology. This chapter discusses the progress of several approaches in parasite diagnosis and some of their silent characteristics. © 2025, Bentham Books imprint.
Effect of nitrogen and zinc fertilizer on zn biofortification in pearlmillet (Pennisetum glaucum)
(Indian Society of Agronomy, 2014) Renu Singh; S.K. Prasad; M.K. Singh
A field experiment was conducted during the rainy (kharif) season of 2011–12 at Mirzapur, Uttar Pradesh to study the response of pearlmillet [Pennisetum glaucum (L.) R. Br.] to nitrogen and zinc on Zn biofortification in this crop. The field experiment was laid out in a factorial randomized block design, with 3 replications, comprising 4 nitrogen (N) levels (0, 20, 40 and 60 kg N/ha) and 3 levels of zinc (0, 5 and 10 kg Zn/ha) with constant rates of phosphorus and potassium at 40 and 30 kg/ha respectively. Application of 60 kg N/ha and 10 kg Zn/ha resulted in the maximum grain yield, N content and uptake by grain and stover of pearlmillet. Zinc content in grain linearly enhanced with sole application of N and Zn levels up to 20 kg N/ha and 5 kg Zn/ha. Combined application of 5 kg Zn/ ha × 20 kg N/ha was found optimum for enhancement of Zn content in grains of pearlmillet. Agronomic nitrogen and zinc efficiency decreased with the increasing nitrogen and zinc levels. © 2014, Indian Society of Agronomy. All rights reserved.
From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network
(Elsevier Ireland Ltd, 2015) Renu Singh; Yashi Singh; Suchit Xalaxo; S. Verulkar; Neera Yadav; Shweta Singh; Nisha Singh; K.S.N. Prasad; K. Kondayya; P.V. Ramana Rao; M. Girija Rani; T. Anuradha; Y. Suraynarayana; P.C. Sharma; S.L. Krishnamurthy; S.K. Sharma; J.L. Dwivedi; A.K. Singh; P.K. Singh; Nilanjay; N.K. Singh; Rajesh Kumar; S.K. Chetia; T. Ahmad; M. Rai; P. Perraju; Anita Pande; D.N. Singh; N.P. Mandal; J.N. Reddy; O.N. Singh; J.L. Katara; B. Marandi; P. Swain; R.K. Sarkar; D.P. Singh; T. Mohapatra; G. Padmawathi; T. Ram; R.M. Kathiresan; K. Paramsivam; S. Nadarajan; S. Thirumeni; M. Nagarajan; A.K. Singh; Prashant Vikram; Arvind Kumar; E. Septiningshih; U.S. Singh; A.M. Ismail; D. Mackill; Nagendra K. Singh
Rice is a staple cereal of India cultivated in about 43.5Mha area but with relatively low average productivity. Abiotic factors like drought, flood and salinity affect rice production adversely in more than 50% of this area. Breeding rice varieties with inbuilt tolerance to these stresses offers an economically viable and sustainable option to improve rice productivity. Availability of high quality reference genome sequence of rice, knowledge of exact position of genes/QTLs governing tolerance to abiotic stresses and availability of DNA markers linked to these traits has opened up opportunities for breeders to transfer the favorable alleles into widely grown rice varieties through marker-assisted backcross breeding (MABB). A large multi-institutional project, "From QTL to variety: marker-assisted breeding of abiotic stress tolerant rice varieties with major QTLs for drought, submergence and salt tolerance" was initiated in 2010 with funding support from Department of Biotechnology, Government of India, in collaboration with International Rice Research Institute, Philippines. The main focus of this project is to improve rice productivity in the fragile ecosystems of eastern, northeastern and southern part of the country, which bear the brunt of one or the other abiotic stresses frequently. Seven consistent QTLs for grain yield under drought, namely, qDTY1.1, qDTY2.1, qDTY2.2, qDTY3.1, qDTY3.2, qDTY9.1 and qDTY12.1 are being transferred into submergence tolerant versions of three high yielding mega rice varieties, Swarna-Sub1, Samba Mahsuri-Sub1 and IR 64-Sub1. To address the problem of complete submergence due to flash floods in the major river basins, the Sub1 gene is being transferred into ten highly popular locally adapted rice varieties namely, ADT 39, ADT 46, Bahadur, HUR 105, MTU 1075, Pooja, Pratikshya, Rajendra Mahsuri, Ranjit, and Sarjoo 52. Further, to address the problem of soil salinity, Saltol, a major QTL for salt tolerance is being transferred into seven popular locally adapted rice varieties, namely, ADT 45, CR 1009, Gayatri, MTU 1010, PR 114, Pusa 44 and Sarjoo 52. Genotypic background selection is being done after BC2F2 stage using an in-house designed 50K SNP chip on a set of twenty lines for each combination, identified with phenotypic similarity in the field to the recipient parent. Near-isogenic lines with more than 90% similarity to the recipient parent are now in advanced generation field trials. These climate smart varieties are expected to improve rice productivity in the adverse ecologies and contribute to the farmer's livelihood. © 2015 Elsevier Ireland Ltd.
The Role of Biochar in Sustainable Agriculture
(Springer Science+Business Media, 2025) Manoj N. Shrivastava; Renu Singh; S. Srivastava
Biochar, a carbonized biomass derived from sustainable sources, offers significant potential for improving soil fertility and mitigating climate change. This chapter explores biochar production from urban waste, focusing on its agricultural applications. Urban waste, particularly municipal solid waste, presents a viable feedstock due to its abundant organic and inorganic components. The chapter details the pyrolysis process, a critical method for biochar production, highlighting its efficiency and environmental benefits. Biochar’s porous nature enhances soil water retention, nutrient availability, and microbial activity, thus improving crop yields and quality. Furthermore, biochar sequesters carbon, reduces greenhouse gas emissions, and decreases reliance on chemical fertilizers. Traditional and modern biochar production techniques are reviewed, emphasizing optimizing conditions to maximize yield and quality upon agricultural use. Challenges such as lack of awareness, technical barriers, and environmental concerns are discussed alongside potential solutions. The chapter underscores biochar’s role in sustainable agriculture, especially in developing countries, where it can contribute to food security, reduce deforestation, and promote cleaner cooking practices. Integrating biochar systems into agricultural practices can realize significant environmental, economic, and social benefits. © 2025 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.