Browsing by Author "Renu Yadav"

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Comparative Study of Trichoderma BHU-1 Mediated Drought Alleviation of Susceptible and Tolerant Rice Cultivars
(Journal of Pure and Applied Microbiology, 2025) Ram Nandan Yadav; Renu Yadav; Najam Waris Zaidi; Harikesh Bahadur Singh; Prashant Bisen; Md Mahtab Rashid; Sudheer Kumar Yadav
The study investigated Trichoderma-mediated morphological and biochemical responses in droughtsusceptible and tolerant rice cultivars, namely IR-64 and DRR-44, under drought-stressed and normal conditions. Various morphological and biochemical parameters were recorded 30, 60, and 90 days after transplanting. The shoot length was insignificant, while the root length was significant in droughtsusceptible DRR-44 compared to non-stressed plants. The number of roots was also significant in Trichoderma BHU-1 treated plants of both cultivars. Proline content was more substantial in drought susceptible cultivars than tolerant and similarly, lignin, TPC, PAL, and PO activities were higher in Trichoderma BHU-1 treated drought-stressed plants than in normal ones. The result revealed that Trichoderma BHU-1 treatment modulates an increase in root length, shoot length, and the total number of tillers and roots under drought conditions. It also maintained the level of phenolics in plants by upregulating the pathway thereby helping the plant to sustain drought. © The Author(s) 2025.
Symbiotic nitrogen fixation for sustainable chickpea yield and prospects for genome editing in changing climatic situations
(Frontiers Media SA, 2025) Rohit Kumar Mahto; B. S. Chandana; Raj Kumar Singh; Arun Kumar; S. Dasaratha Kumar; Renu Yadav; Debashish Dey; Aladdin Hamwieh; Rajendra A.J. Kumar
Chickpea (Cicer arietinum L.) is a vital/essential legume crop valued for its nutritional, agricultural, and economic importance, with a relatively large genome size of approximately 738 megabases. Chickpea roots establish symbiotic relationships with soil microorganisms, resulting in the formation of root nodules essential for biological nitrogen fixation. In this study, 20 chickpea genotypes were selected from a genome-wide association panel to assess nodulation traits under eight different treatment combinations involving biofertilizers (Rhizobium, vesicular-arbuscular mycorrhiza - VAM) and inorganic fertilizers (NPK) using a randomized block design with three replications. Pre-planting soil preparation included the application of fertilizers and biofertilizers. Comprehensive analyses including descriptive statistics, correlation, path analysis, principal component analysis, agglomerative hierarchical clustering, and gene expression studies were conducted. Among treatments, the NPK+Rhizobium combination significantly enhanced nodulation across genotypes, while the Rhizobium+VAM (T7) treatment identified ICC-9085 as a superior donor for the number of nodules, aiming for sustainable chickpea productivity. Gene expression profiling through qRT-PCR revealed that the RZ+VAM treatment notably upregulated several key genes, including CaNFP, GST, Leghemoglobin, Nodulin6, and CaLYK3, with CaNFP emerging as a pivotal regulator of nodulation. The marked upregulation of CaNFP underlines its potential as a target for enhancing symbiotic efficiency. The availability of the chickpea draft genome opens new avenues for employing genome editing tools such as CRISPR/Cas systems. Targeted editing of the CaNFP gene offers a promising strategy to improve nodule formation, nitrogen fixation, and overall plant vigor. Integrating CaNFP gene through genome editing with potential genotypes and use of microbial treatments can accelerate the development of elite chickpea cultivars, enhancing productivity while reducing reliance on chemical fertilizers and supporting sustainable agricultural practices. © © 2025 Mahto, B. S., Singh, Kumar, Kumar, Yadav, Dey, Hamwieh and Kumar.