Browsing by Author "Riden Saxena"

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Ab initio modeling and ligand docking of quercetin and the MC-LR transporter protein Oatp1b2/OATP1B3
(Elsevier B.V., 2023) Kriti Shrinet; Ritika K. Singh; Riden Saxena; Avinash K. Chaurasia; Arvind Kumar
Trans-membrane proteins (TMPs) play a crucial role in the translocation of organic and inorganic molecules. Unlike other proteins, TMPs are difficult to model structurally because of their location within the amphipathic plasma membrane. In this study, we focused on examining the transport of the cyanotoxin microcystin-LR (MC-LR) through organic ion transporting polypeptides (OATPs) and whether the bioactive phytoconstituent quercetin can function as a barrier to the transportation of MC-LR. To test this hypothesis, we first modeled the transporters OATP1B3 and Oatp1b2 localized in the human and mouse liver, respectively, by ab initio modeling with the Iterative Threading ASSEmbly Refinement server and refined the generated model using the refinement tool of the ModLoop server. Using different tools and servers, the structural quality of the transmembrane helices was validated and found to be an accurate structure of a TMP. Docking analysis was performed with the ligands MC-LR and quercetin with both OATPs using the PatchDock and FireDock online servers. The results, in the form of the global energy of both docked structures, were based on predictions made earlier. The Oatp1b2 global energy for quercetin was −36.4 kcal/mol, compared with the corresponding value at the MC-LR location which was only −5.59 kcal/mol. Similarly, in the case of OATP1B3 with quercetin, the global energy was found to be −39.0 kcal/mol, whereas with MC-LR it was −15.6 kcal/mol. These results clearly show that quercetin competitively inhibits the binding of MC-LR to its respective targets. © 2023 The Authors
Development and evaluation of herbal tablet loaded with Pueraria tuberosa water extract with use of different excipients
(BRNSS Publication Hub, 2018) Harsh Pandey; Shivani Srivastava; Brahmeshwer Mishra; Riden Saxena; Yamini Bhusan Tripathi
Aim: The aim of present paper is to develop the best quality of low cost tablets and with better effectiveness . Material and Methods: In this experiment we have used starch and some other excipients such as Microcrystalline cellulose, Acacia, Gellatin, Polyvinylpyrolidone and sodium Aliginate. The Water extract of plant Pueraria tuberosa belonging to the family of fabeacea. The herbal tablet of PT was made by wet granulation method. The finished products were characterized by standered method. For the determination of concentration, we have drawn the standard calibration curve of PT extract having absorption maxima at 221 nm in 0.1 N HCL solution. The drug obeyed Beer's lamberd law in the concentration range of 10 µg/ml to 50 µg/ml and was found to be linear with r2 =0.988 and regression equation y= 0. 003x- 0.009. Result and Discussion: The parameters of pre formulation such as Angle of repose, Carr's index and Husnner ratio showed good flow property. Tablets were evaluated by both official and non official testing, according to Indian Pharmacopoeia. After the official testing, the tablets made up by starch were not shown any significant change in properties . The friability of all formulations is less than 1%, except F0. Out of all, one of our formulation of herbal tablet mixed with 5% starch (FA) showed good release property within 1 hour in dissolution media, studied through in vitro method. Conclusion: These results concludes that starch can be proved as the better excipient for preparation of herbal tablets. © 2018 BRNSS Publication Hub. All rights reserved.
Diagnosis of Genital Tuberculosis in Infertile Women by Using the Composite Reference Standard
(Hindawi Limited, 2022) Riden Saxena; Kriti Shrinet; Sachchida Nand Rai; Kamal Singh; Shivi Jain; Shuchi Jain; Deeksha Singh; Shampa Anupurba; Madhu Jain
Female genital tuberculosis (FGTB) can be asymptomatic or even masquerade as other gynecological conditions. Conventional methods of FGTB diagnosis include various imaging, bacteriological, molecular, and pathological techniques that are only positive in a small percentage of patients, leaving many cases with undiagnosed condition. In the absence of a perfect diagnostic method, composite reference standards (CRSs) have been advocated in this diagnostic study. This study assesses the agreement between traditional diagnostic modalities using CRS and prevalent TB groups among different fallopian tube infertility manifestations. A total of 86 women with primary and secondary infertility were included in the study and subjected to bacteriological, pathological, and radiological examination for the diagnosis of FGTB. Results were evaluated statistically for concordance of the diagnostic tests to the CRS by sensitivity and specificity, while PPV and NPV were calculated for the performance of diagnostic tests of FGTB. We observed that 11.2% of women were found to be true positives by means of CRS. The positive findings by CRS were as follows: ultrasonography (13.9%), laparoscopy (14%), hysteroscopy (12%), GeneXpert (4.8%), culture (4.8%), polymerase chain reaction (4.8%), and histopathology (6.4%). GeneXpert and culture were found to have a perfect agreement with CRS. Hysterosalpingography, laparoscopy, and hysteroscopy have a fair agreement with CRS. Out of 43 women with tubal factor infertility, 6 women were found in the definitive TB group with mixed conditions of tubal manifestations. This study evaluates and demonstrates the reliability of the collective assessment of various diagnostic methods with CRS findings that help in identifying different TB groups of genital tuberculosis patients from all infertile patients by applying the criteria of CRS. © 2022 Riden Saxena et al.
Effect of Orlistat Versus Metformin in Various Aspects of Polycystic Ovarian Syndrome: A Systematic Review of Randomized Control Trials
(Federation of Obstetric and Gynecologycal Societies of India, 2018) Soumya Ranjan Panda; Madhu Jain; Shuchi Jain; Riden Saxena; Smrutismita Hota
Background: Polycystic ovarian syndrome (PCOS), a commonly prevalent endocrinopathy among reproductive age group women, is most often associated with obesity. Increased insulin resistance appears to be the central pathophysiologic mechanism responsible for various complications of PCOS. This makes ‘weight loss’ as the first-line treatment approach in PCOS. So various trials have tried to compare metformin (an insulin-sensitizing agent) and orlistat (an anti-obesity drug) aiming to achieve weight loss and hence higher ovulation rate for the group of obese PCOS patients. Keeping an eye on all these background facts, we designed this systematic review and metaanalysis to compare the effects of metformin and orlistat on various aspects of PCOS and to pick the better among the two drugs. Materials and Methods: This is a systemic review of randomized control trials that studied the effectiveness of orlistat versus metformin in terms of improvement in ovulation rate, weight loss, lipid profile, etc. Systematic literature search over the period January 2000–December 2016 was performed in the following electronic databases: Medline, embase, google scholar, pubmed and The Cochrane Library and only randomized controlled clinical trials were included in our study. All authors carefully went through all sources of information independently. Results: According to this study, weight loss, testosterone level after 4 weeks of treatment, total serum cholesterol and triglyceride level showed significant fall in orlistat-treated group. Conclusion: Our review shows that orlistat is a more effective drug than metformin and should be the preferred drug in obese PCOS in combination with weight loss. © 2018, Federation of Obstetric & Gynecological Societies of India.
GC-MS Profiling of Phytobioconstituents Present in Andrographis paniculata (Burm.f.) Nees Extract and Pharmacokinetic and Toxicity Properties by ADME/Tox Analysis
(Asian Publication Corporation, 2022) Riden Saxena; Kriti Shrinet; Ritika Saxena; Yatender Singh Panwar; Madhu Jain
Andrographis paniculata, commonly known as the "King of Bitters" has traditionally been used as a medicinal herb. The metabolite composition of A. paniculata is generally determined by its properties, which may be influenced by a range of factors, one of which is the part of the plant extracted. The objective of this research is to identify and characterize the putative metabolite composition in n-hexane extract of stem and leaf using gas chromatography-mass spectrometry. A total of 22 different phytobioconstituents were identified, including flavonoids, terpenoids, fatty acids, phenolic acids, quinones, alkaloids and vitamin. The pharmacokinetic properties of the identified phytobioconstituents, like drug-likeness and toxicity, were also predicted by ADME/Tox analysis. Phytobioconstituents, predicted to be drug-like can be further investigated as ligands by using in silico docking approaches, for disease targets. The study provides empirical evidence for future study that might lead to their therapeutic applications and industrial product development. © 2022 Chemical Publishing Co.. All rights reserved.