Browsing by Author "Romaan Nazir"

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Biotechnological interventions of in vitro propagation and production of valuable secondary metabolites in Stevia rebaudiana
(Springer Science and Business Media Deutschland GmbH, 2021) Shahnawaz; Devendra Kumar Pandey; Merinashwari Konjengbam; Padmanabh Dwivedi; Prabhjot Kaur; Vijay Kumar; Durga Ray; Puja Ray; Romaan Nazir; Harmeet Kaur; Sidharth Parida; Abhijit Dey
Abstract: Plant cell and tissue culture makes provision of a sustainable and nature-friendly strategy for the production of secondary metabolites, and modern progress in gene editing and genome engineering provides novel possibilities to improve both the qualitative and quantitative aspects of such phytochemicals. The ever-expanding quest for plant-based medicine to treat diabetes facilitates large-scale cultivation of Stevia rebaudiana to enhance the yield of its much-coveted low-calorie sweetener glycosides. The potential to process stevia as a “natural” product should enhance the acceptance of steviosides as a natural calorie-free sweetener especially suitable for use in diabetic and weight control drinks and foods. Besides sweetener agents, S. rebaudiana is a potent source of many antioxidant compounds and is used to cure immunodeficiencies, neurologic disorders, inflammation, diabetes mellitus, Parkinson’s disease, and Alzheimer’s disease. This comprehensive review presents the research outcomes of the many biotechnological interventions implicated to upscale the yield of steviol glycosides and its derivatives in in vitro cell, callus, tissue, and organ cultures with notes on the use of bioreactor and genetic engineering in relation to the production of these valuable compounds in S. rebaudiana. Key points: • Critical and updated assessment on sustainable production of steviol glycosides from Stevia rebaudiana. • In vitro propagation of S. rebaudiana and elicitation of steviol glycosides production. • Genetic fidelity and diversity assessment of S. rebaudiana using molecular markers. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Biotechnological strategies for the sustainable production of diosgenin from Dioscorea spp.
(Springer Science and Business Media Deutschland GmbH, 2021) Romaan Nazir; Vijay Kumar; Suphala Gupta; Padmanabh Dwivedi; Devendra Kumar Pandey; Abhijit Dey
Abstract: Diosgenin is a plant-derived secondary metabolite mainly present in the members of the plant family Dioscoreaceae. It is a pharmaceutically important compound because of its anti-cancerous, anti-diabetic, anticoagulant, anti-thrombosis, anti-inflammatory, anti-viral, anti-ageing and other properties. Biotechnology provides an opportunity to genetically manipulate cells, tissues, organs or the whole organisms by propagating them in vitro in order to harvest the bioactive compounds. Diosgenin production from botanical sources is being improved by in vitro techniques which include elicitation, genetic transformations and bioconversions. Various techniques have been developed to obtain compounds for drug detection including separation from plants and other natural sources, molecular modelling, synthetic chemistry and combinatorial chemistry. Development in molecular markers determines genetic relationship, genetic linkage map construction, genetic diversity and identification. For rapid clonal propagation and ex situ conservation, the in vitro tools involving plant cell, tissue and organ culture have been well documented for plant-derived diosgenin production. The present review encompasses the wide application of the biotechnological techniques for diosgenin production via elucidating its biosynthetic pathway, in vitro production and mass propagation and elicitation. In addition, molecular marker-mediated diversity assessment of diosgenin containing plant species is also discussed. The review also presents the recent literature to explore the limitations of the relevant studies and future direction of research on production of diosgenin from Dioscorea spp. Key points: • Critical and updated assessment on sustainable production of diosgenin from Dioscorea spp. • In vitro propagation of Dioscorea spp. and elicitation of diosgenin production. • Diversity assessment of Dioscorea spp. using molecular markers. Graphical abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
In vitro propagation and assessment of genetic fidelity in Dioscorea deltoidea, a potent diosgenin yielding endangered plant
(Elsevier B.V., 2021) Romaan Nazir; Suphla Gupta; Abhijit Dey; Vijay Kumar; Muzamil Yousuf; Shahnawaz Hussain; Padmanabh Dwivedi; Devendra Kumar Pandey
Dioscorea deltoidea (Family: Dioscoreaceae) is a high value endangered medicinal plant and the source of pharmaceutically important bioactive compound diosgenin, which is used in the synthesis of steroidal drugs. Establishing an efficient reproducible regeneration system is necessary for its sustainable utilization and conservation. The purpose of this research was to establish an effective and efficient in vitro regeneration protocol for D. deltoidea and assess its genetic and biochemical fidelity. In this report, Murashige and Skoog (MS) medium fortified with different types and concentration of Plant Growth Regulators (PGRs) were studied. Maximum shoot regeneration frequency was achieved in MS medium supplemented with 2.0 mg L − 1 BA whereas higher shoot multiplication was observed in 2.0 mg L − 1 BA + 1.0 mg L − 1 IBA. Highest rooting was recovered on MS medium supplemented with 2.0 mg L − 1 NAA. In vitro rooted plantlets were acclimatized with a survival rate of 96%. The genetic fidelity of the mother plant (MP), acclimatized plant (AP) and in vitro regenerants were found to be clonally similar as examined through ISSR fingerprinting. Of 7 ISSR primers tested, 5 produced scorable amplified products. The High Performance Thin Layer Chromatography (HPTLC) based chemical assessment showed varied chemical profile. The developed protocol would be beneficial in providing a germplasm conservation system, isolation of secondary metabolites and production of high value clonal regenerants for commercial production. © 2020 SAAB
Optimization of diosgenin extraction from Dioscorea deltoidea tubers using response surface methodology and artificial neural network modelling
(Public Library of Science, 2021) Romaan Nazir; Devendra Kumar Pandey; Babita Pandey; Vijay Kumar; Padmanabh Dwivedi; Aditya Khampariya; Abhijit Dey; Tabarak Malik
Introduction Dioscorea deltoidea var. deltoidea (Dioscoreaceae) is a valuable endangered plant of great medicinal and economic importance due to the presence of the bioactive compound diosgenin. In the present study, response surface methodology (RSM) and artificial neural network (ANN) modelling have been implemented to evaluate the diosgenin content from D. deltoidea. In addition, different extraction parameters have been also optimized and developed. Materials and methods Firstly, Plackett-Burman design (PBD) was applied for screening the significant variables among the selected extraction parameters i.e. solvent composition, solid: Solvent ratio, particle size, time, temperature, pH and extraction cycles on diosgenin yield. Among seven tested parameters only four parameters (particle size, solid: Solvent ratio, time and temperature) were found to exert significant effect on the diosgenin extraction. Moreover, Box-Behnken design (BBD) was employed to optimize the significant extraction parameters for maximum diosgenin yield. Results The most suitable condition for diosgenin extraction was found to be solid: Solvent ratio (1:45), particle size (1.25 mm), time (45 min) and temperature (45°C). The maximum experimental yield of diosgenin (1.204% dry weight) was observed close to the predicted value (1.202% dry weight) on the basis of the chosen optimal extraction factors. The developed mathematical model fitted well with experimental data for diosgenin extraction. Conclusions Experimental validation revealed that a well trained ANN model has superior performance compared to a RSM model. © 2021 Nazir et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Response surface methodology and artificial neural network modeling for optimization of ultrasound-assisted extraction and rapid HPTLC analysis of asiaticoside from Centella asiatica
(Elsevier B.V., 2022) Poonam Kumari; Prabhjot Kaur; Vijay Kumar; Babita Pandey; Romaan Nazir; Kajal Katoch; Padmanabh Dwivedi; Abhijit Dey; Devendra Kumar Pandey
The present study optimizes various extraction conditions for better yield of asiaticoside in Centellaasiatica. Response surface methodology (RSM) and artificial neural network (ANN) were used for the first time here in order to model and optimize the ultrasonic extraction parameters of asiaticoside from C.asiaticaleaves for comparing and establishment of effective prediction models.The quantitative determination of asiaticoside was carried out on silica gel 60 F254HPTLC plates by using the mobile phase consisting of butanol: ethyl acetate: water (4:1:5). The optimum sonication parameters solid:solvent ratio (1:15), sonication time (18 min), solvent composition (35% aqueous-ethanol), the experimental maximum yield obtained for asiaticoside were 0.198% and the maximum predicted yield were found to be 0.201% i.e closely related to the experimental yield. The results showed that RBF gives better performance as compared to MLP and RSM. The study suggests that RSM and ANN model system can be manipulated for the optimization and production of valuable bioactive compounds. © 2021 Elsevier B.V.
RETRACTED ARTICLE: A response surface-based approach to optimize elicitation conditions in Stevia rebaudiana (Bertoni) Bertoni shoot cultures for the enhancement of steviol glycosides(Plant Cell Tissue and Organ Culture, (2023), 155, 6, (1-11))
(Springer Science and Business Media B.V., 2023) Shah Nawaz; Prabhjot Kaur; Merinaswari Konjengbam; Vijay Kumar; Romaan Nazir; Padmanabh Dwivedi; Tuyelee Das; Abhijit Dey; Devendra Kumar Pandey
The Editor-in-Chief has retracted this article because of signifcant concerns regarding two fgures presented in this work, which question the integrity of the data. After publication, a number of overlaps were detected within Figure 1 of this article, and some of the images in this fgure appear to have been cropped to give the appearance of being diferent samples. Specifcally:• samples 2, 6, and 9 appear to be identical• samples 3, 7, and 10 appear to be identical• samples 5 and 8 appear to be identical Also, in Figure 6, lines 17–20 seem identical to lines 9–12.The Editor-in-Chief therefore no longer has confdence in the integrity of the data in this article.All authors agree with this retraction. © 2023 Springer Nature B.V.
Retraction notice:In vitro propagation and assessment of genetic fidelity in Dioscorea deltoidea, a potent diosgenin yielding endangered plant (South African Journal of Botany (2021) 140 (349), (S0254629920309960), (10.1016/j.sajb.2020.07.018))
(Elsevier B.V., 2024) Romaan Nazir; Suphla Gupta; Abhijit Dey; Vijay Kumar; Muzamil Yousuf; Shahnawaz Hussain; Padmanabh Dwivedi; Devendra Kumar Pandey
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief as the reliability of the peer-review process of the article cannot be guaranteed. After a thorough investigation, the Editor and publisher have concluded that the Managing Guest Editor of the Special Issue, Dr. Vijay Kumar, was also an author of the article and involved in the peer review and decision making. As such this represents an abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process. © 2020 South African Association of Botanists