Browsing by Author "Ruchita Tripathi"

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Characterization and evaluation of mycosterol secreted from endophytic strain of Gymnema sylvestre for inhibition of α-glucosidase activity
(Nature Research, 2019) Amit Ranjan; Rajesh Kumar Singh; Saumya Khare; Ruchita Tripathi; Rajesh Kumar Pandey; Anurag Kumar Singh; Vibhav Gautam; Jyoti Shankar Tripathi; Santosh Kumar Singh
Endophytic fungi produce various types of chemicals for establishment of niche within the host plant. Due to symbiotic association, they secrete pharmaceutically important bioactive compounds and enzyme inhibitors. In this research article, we have explored the potent α-glucosidse inhibitor (AGI) produced from Fusarium equiseti recovered from the leaf of Gymnema sylvestre through bioassay-guided fraction. This study investigated the biodiversity, phylogeny, antioxidant activity and α-glucosidse inhibition of endophytic fungi isolated from Gymnema sylvestre. A total of 32 isolates obtained were grouped into 16 genera, according to their morphology of colony and spores. A high biodiversity of endophytic fungi were observed in G. sylvestre with diversity indices. Endophytic fungal strain Fusarium equiseti was identified through DNA sequencing and the sequence was deposited in GenBank database (https://ncbi.nim.nih.gov) with acession number: MF403109. The characterization of potent compound was done by FTIR, LC-ESI-MS and NMR spectroscopic analysis with IUPAC name 17-(5-ethyl-6-methylheptan-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a] phenanthren-3-ol. The isolated bioactive compound showed significant α-amylase and α-glucosidase inhibition activity with IC50 values, 4.22 ± 0.0005 µg/mL and 69.72 ± 0.001 µg/mL while IC50 values of acarbose was 5.75 ± 0.007 and 55.29 ± 0.0005 µg/mL respectively. This result is higher in comparison to other previous study. The enzyme kinetics study revealed that bioactive compound was competitive inhibitor for α-amylase and α-glucosidase. In-silico study showed that bioactive compound binds to the binding site of α-amylase, similar to that of acarbose but with higher affinity. The study highlights the importance of endophytic fungi as an alternative source of AGI (α-glucosidase inhibition) to control the diabetic condition in vitro. © 2019, The Author(s).
Fungi as potential candidates for bioremediation
(Elsevier, 2019) Rajesh Kumar Singh; Ruchita Tripathi; Amit Ranjan; Akhileshwar Kumar Srivastava
The accumulations of waste and hazardous material have often increased toxicity level in nature, causing several adverse effects including human health. At present, the incineration is widely used as common method for remediation; however, it has several limitations. One of the alternative approaches to incineration is bioremediation that exploits the potential microorganisms for abolishing the accumulated pollutants in nature. Fungi are among potential candidates producing various hydrolyzing enzymes that play a significant role in decomposing the waste materials and adapt to different environmental conditions which enables them to survive in diverse condition. Therefore, it is required to identify the fungal species targeting to certain pollutant for achieving an effective mycoremediation. This chapter describes the different fungal groups with their habitat and potential role in bioremediation of hazardous material occurring in environment. The several fungal strains in nature are producing numerous enzymes, viz. hydrolases, lyases, transferases, and oxidoreductases, and decomposing and detoxifying the different pollutants and materials. The application of fungi in bioremediation for different toxic waste materials with details of the bioremediation pathways and fungal stains has also been described for the development of potential technology for removal of pollutant and toxic materials from environment. © 2020 Elsevier Inc. All rights reserved.
GC-MS and NMR spectroscopy based metabolite profiling of Panchvalkal kwath (polyherbal formulation)
(Taylor and Francis Ltd., 2023) Pratistha Singh; Renuka Ranjan; Ruchita Tripathi; Jyoti Dixit; Neeraj Sinha; Anil Kumar Singh; Kavindra Nath Tiwari
Panchvalkal kwath (PK) is a bark formulation of five pharmacologically important plants, i.e., Ficus benghalensis, Ficus racemosa, Ficus religiosa, Thespesia populnea, and Ficus lacor. The Ayurvedic formulation is being used since ancient times to cure diabetes, bacterial infections and heal wounds. The present study aims to identify the metabolite profiles of PK which could explain its properties and its mode of action against specific diseases and disorders. The aqueous extract of Panchvalkal is prepared through a hot maceration process. The extract is subjected to preliminary identification of phytoconstituents and FTIR spectroscopy to recognize functional groups. GC-MS analysis reveals that the extract is enriched with 24-Norursa-3,12-diene (25.16%); Lup-20(29)-en-3-one (16.76%); 2-methyl-3-(4-propan-2-ylphenyl) propanal (7.04%); 2-(hydroxymethyl)-2-nitropropane-1,3-diol (11.21%) and 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one (4.15%). The presence of three new phytocompounds that are 4-(hydroxymethyl)-7-methyl-1,3-dioxepane-5,6-diol; 1-(4-isopropylphenyl)-2-methylpropylacetate and 4,4,6 A,6B,8A,11,11,14B-octamethyl-1,4,4A,5,6,6A,8,8a,910,11,12,12a,12b,13,14,14a,14b-ctadecahydro-3(2H)-picenone are detected in the extract. Metabolite profiles of the extract also constitute isoeugenol, stigmasterol, ergosterol, ocimene, myrcene, squalene, sphingosine, betulin, methyl ferulate and cis-jasmone, which are unraveled by 1 D 1H and 2 D 1H-13C HSQC NMR spectroscopy. This article focuses on the presence of different phytocompounds in PK in order to demonstrate its efficacy as a therapeutic formulation for a variety of diseases. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Panchvalkal (an Ayurvedic Formulation) Proliferates Pancreatic β-Cells and Increases the Potency of Conventional drug Glibenclamide
(Springer, 2023) Ruchita Tripathi; Amit Ranjan; Raghvendra Pratap; Anil Kumar Singh; Rajesh Kumar Singh
Diabetes is a common metabolic syndrome in Indian population. The drugs used for its management based on enzyme and hormone. The development of resistance against conventional drugs in diabetic patients is a major challenge for clinicians to treat the patients effectively. Panchavalkala is a polyherbal formulation, described in Ayurveda for management of many clinical ailments and potentiating the antibacterial drugs in recent studies. In this study it has been evaluated for integration with conventional drug, Glibenclamide for potentiating the efficacy and safety for management of patient with diabetes mellitus in north Indian population. The methodology of Panchvalkala kwath preparation is according to protocol described in Ayurvedic Pharmacopoiea of India (API) and evaluated for safety and efficacy alone and integrated with conventional drug, Glibenclamide in in vitro as well as in-vivo model. The waster rats as used as in-vivo model with Streptozotocin diabetes induction. The results show that the integration of the Ayurvedic formulation is safe and potentiating the efficacy of Glibenclamide. © 2023, The Author(s), under exclusive licence to The National Academy of Sciences, India.
Prophyletic origin of algae as potential repository of anticancer compounds
(Elsevier Science Ltd., 2020) Ruchita Tripathi; Rachana Shalini; Rajesh Kumar Singh
Algae are photosynthetic eukaryotic organism which is a promising source of therapeutic bioactive compounds, used in treatment of many clinical ailments such as obesity, cancer, diabetes, inflammation, neurodegenerative diseases, viral infection, microbial infections, etc. Several studies on algal genera especially on Chlorophyceae, Pheophyceae, and Rhodophyceae have reported their potential use as antiproliferative, antiangiogenic, apoptotic-inducer and cytotoxic. The algal compounds such as some polysaccharide, glycoprotein, siphonaxanthin, plocornulides, sodwanone, stypodiol diacetate, cannabinoids, etc. have well studied using different cancer cell lines and they showed promising results for cancer treatment and improvement of quality of life of the patients. The molecular mechanisms of algal bioactive compound as anticancer include dysregulation of the mitochondrial dynamics, caspases activation, amplification of death signals through death receptors, etc. These mechanisms may support to defeat the multifaceted of cancerous cells. In this chapter, the anticancer property of algal species belongs to different algal genera and their bioactive compounds have discussed with relevant mechanism of actions. © 2021 Elsevier Inc. All rights reserved.
Semecarpus anacardium L.f. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice
(Taylor and Francis Ltd., 2024) Rajesh Kumar Singh; Bhagaban Mallik; Amit Ranjan; Ruchita Tripathi; Sumit Singh Verma; Vinamra Sharma; Subash Chandra Gupta; Anil Kumar Singh
Semecarpus anacardium L.f. has been commonly used in various traditional medicines from ancient times. The nuts have been described in Ayurveda medication systems to treat numerous clinical ailments. However, isolating phytochemical constituents from nuts remain challenging and exhibits cytotoxic effects on other cells. In this study, we have standardized procedures for isolating phytochemicals from the leaf extract. The ethyl acetate leaf extract selectively affects cancer cells in a dose-dependent manner (IC50: 0.57 µg/ml in MCF-7 cells) in various cancer cell lines and induces apoptosis in cancer cells. However, the non-malignant cells were relatively insensitive to the extract. Next, the incubation of the leaf extract induces cell cycle arrest and suppresses cancer cell migration in the cell culture model. Moreover, oral administration of extract significantly restored tumor growth in mice. Together, these observations suggest the anti-cancer activities of S. anacardium L.f. leaf potential for both in vitro and in vivo models. © 2023 Informa UK Limited, trading as Taylor & Francis Group.