Browsing by Author "S. Kumari"

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A study of knowledge and traditional wisdom of makhana cultivation in bihar
(National Institute of Science Communication and Information Resources, 2021) A. Kumar; A.K. Singh; B. Kumar; S. Kumari
Makhana (Euryale ferox) is an aquatic crop from the family Nymphaeace and is commonly grown in stagnant water bodies like ponds, swamps and ditches with shallow water. Euryale is a self-pollinated plant, in which pollination takes place at the early stage of flower development. Bihar is the largest producer of makhana throughout the country with an area of approx 15,000 ha. In spite of several uses and immense market potential in India and abroad, makhana is still cultivated through traditional system, area of which is declining in recent years. Therefore, an attempt was made to understand traditional wisdom of makhana production practices with special reference to its post harvest management. For this purpose, 100 makhana growers were identified from Madhubani district of Bihar which occupies more than 30% of the total area and production. They were personally interviewed with the help of a structured schedule consisting of different components such as, suitable soils in terms of type, quality and its strength, the type, slope and depth of ponds of makhana, the preparation and management of pond for makhana cultivation, seeds with respect to source and quality, fertilizer and disease management, harvesting process of makhana with respect to its the time and process of maturity along with the grading and marketing of makhana. The results indicated that makhana production practices are primarily governed with the traditional knowledge system which the growers obtained through their forefathers. © 2021, National Institute of Science Communication and Information Resources. All rights reserved.
Burden of different beta-lactamase classes among clinical isolates of AmpC-producing Pseudomonas aeruginosa in burn patients: A prospective study
(Wolters Kluwer Medknow Publications, 2012) V. Kumar; M.R. Sen; C. Nigam; R. Gahlot; S. Kumari
Background: Pseudomonas aeruginosa is one of the most common pathogens causing infections in burns, and shows increasing resistance to -lactam antibiotics by producing different classes of beta-lactamases. It is also not unusual to find a single isolate that expresses multiple -lactamase enzymes, further complicating the treatment options. Thus, in this study, we aimed to determine the coexistence of different beta-lactamase enzymes in clinical isolates of P. aeruginosa in the burn ward. Materials and Methods: A total of 101 clinical isolates of P. aeruginosa from the burn ward were identified and tested for the presence of different beta-lactamase enzymes (extended spectrum beta lactamase (ESBL), Amp C and metallo -lactamases (MBL) from October 2006 to May 2009. In vitro susceptibility pattern of antipseudomonal antibiotics was done by the Kirby Bauer disc diffusion method. Results: A total of 33 (32.7%) isolates were confirmed to be positive for AmpC beta-lactamase. Co-production of AmpC along with ESBL and MBL was reported in 24.5% and 45.5% isolates, respectively. A total of 12 (11.9%) isolates were resistant to three or more antibiotic classes (multidrug resistance). Imipenem and piperacillin/tazobactum showed high sensitivity, with 86.1% and 82.2%, respectively. Conclusion: This study reveals the high prevalence of multidrug- resistant P. aeruginosa producing beta-lactamase enzymes of different mechanisms in this region from burn patients. The emerging antimicrobial resistance in burn wound pathogens poses serious therapeutic challenge. Thus proper antibiotic policy and measures to restrict the indiscriminate use of cephalosporins and carbapenems should be taken to minimize the emergence of this multiple beta -lactamase producing pathogen.
Electrochemical sensing of dopamine over polyindole-composite electrode
(American Ceramic Society, 2011) Prem C. Pandey; Dheeraj S. Chauhan; S. Kumari
We hereby report a new conducting polymer composite i.e., polyindole-camphorsulphonic acid (PIn-CSA) composite, and its use in the development of dopamine sensor. The processable PIn-CSA composite is developed by homogenizing equimolar ratio of chemically synthesized polyindole and camphor sulphonic acid in tetrahydrofuran. The homogenized PIn-CSA composite is cast over the Pt disc electrode under ambient conditions and assembled in a homemade electrode body equipped with Ag/AgCl reference electrode. The ion sensor exhibits excellent response towards dopamine in presence of ascorbic acid over a wide concentration range. The sensor can be used for 1 month without any major drift in its sensitivity and limit of detection.
Expression of p27 and p16 and their clinical significance in gastric cancer
(Springer Science and Business Media Deutschland GmbH, 2021) S. Kumari; P. Kumar; M. Kumar; S. Singh; G. Narayan
Background: Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. p27kip1 and p16INKa are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods: We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results: We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions: The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients. © 2020, Federación de Sociedades Españolas de Oncología (FESEO).
Square planar Pd(II) complexes derived from 1-ethyl-3-phenylthiourea, 3-mercapto-4-methyl-1,2,4-triazole and 2-mercapto-5-methyl-1,3,4-thiadiazole: Syntheses, spectral, structural characterization and photoluminescence properties
(Elsevier S.A., 2016) P. Bharati; A. Bharti; P. Nath; S. Kumari; N.K. Singh; M.K. Bharty
The reaction of PdCl2 with 1-ethyl-3-phenyl-thiourea (Heptu), 3-mercapto-4-methyl-4H-1,2,4-triazole (Hmmtrz) and 2-mercapto-5-methyl-1,3,4-thiadiazole (Hmthd) respectively, yielded three new complexes [Pd(eptu)2] (1), [Pd(Hmmtrz)4]Cl2 (2) and [Pd(Hmthd)4]Cl2·2CHCl3 (3). These complexes have been isolated in pure form and characterized by elemental analyses, IR, NMR and single crystal X-ray diffraction technique. In all compounds the metal ion adopts a square-planar geometry. Two nitrogen and two sulfur atoms from the monoanionic eptu ligand in complex 1 are bonded to Pd(II) centre in trans fashion. Complexes 2 and 3 are salt-like and the metal centre is bonded through four neutral ligands. The electrical neutrality in these complexes is maintained by the presence of two chlorides as counter ions. Migration of hydrogen, within the ligand framework in complexes 2 and 3, from sulfur to nitrogen is observed that resulted in the thione form of the ligand and its coordination to the metal centre in both the complexes. Complex 2 is stabilized by intermolecular C-H⋯N and intramolecular N-H⋯Cl hydrogen bonding leading to an extended structure. Complex 3 is stabilized by intermolecular C-H⋯S hydrogen bonding. Complexes 1-3 are fluorescent materials which upon excitation at 31 000, 38 600 and 32 300 cm-1 exhibit an emission at 25 200, 27 000 and 26 700 cm-1, respectively. © 2016 Elsevier B.V. All rights reserved.