Browsing by Author "S. Pandey Rai"

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Supplemental ultraviolet-B and ozone: Impact on antioxidants, proteome and genome of linseed (Linum usitatissimum L. cv. Padmini)
(2011) R. Tripathi; A. Sarkar; S. Pandey Rai; S.B. Agrawal
The present investigation used Linum usitatissimum L. cv. Padmini (linseed), under field conditions in open-top chambers, to evaluate the interactive effects of supplemental ultraviolet-B (sUV-B; ambient +7.2 kJ.m-2.d-1) and ozone (O3; ambient +10 ppb). Treatment of plants with sUV-B and O3, individually or in combination, caused several changes in enzymatic and non-enzymatic components of the antioxidant defence system. Photo-oxidative damage caused by sUV-B and O3, included lipid peroxidation, changed protein profiles and caused DNA strand breakage. One-dimensional gel electrophoresis revealed that proteins of 222.24 and 50.5 kDa are specific and appear after sUV-B and O3 exposure, and could be used as indicator proteins. Effects of sUV-B and O3 given separately are more detrimental as compared to combined treatment. Mutational and structural alterations in linseed DNA after these stresses were also examined using RAPD with ten different primers. The study concluded that both stresses, i.e. sUV-B and O3, are phytotoxic, causing significant changes in metabolites, antioxidants, the leaf proteome and the genome of linseed, but their interactive effect was always less than additive. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.