Browsing by Author "S.D. Kharche"

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BONE MARROW BY ALKALINE PHOSPHATASE STAINING
(Indian Society for Sheep and Goat Production and Utilization, 2022) Sonam Rani; D. Jena; S.D. Kharche; R. Nigam; R. Singh
[No abstract available]
CERTAIN ASPECTS OF MESENCHYMAL STEM CELLS IN SMALL RUMINANTS: AN OVERVIEW
(Indian Society for Sheep and Goat Production and Utilization, 2022) Parul Yadav; Dayanidhi Jena; Sonam Rani; S.D. Kharche; J.S. Sohal; G.K. Aseri
In recent years, there has been tremendous progress in the fields of tissue engineering and cellular therapy. Bone tissue engineering is an alternative for regeneration of large bone defects caused by trauma and other bone pathologies. Treatments employed for bone regeneration are based on the use of bioactive molecules, stem cells and scaffold among others. Among these treatments, mesenchymal stem cells (MSCs) have number of advantages: i) available in large quantities from patients (ii) capable of suppressing large immune response and (iii) migrate to sites of injury. A number of farm animal models have been used for tissue-engineered products. Within each animal model, studies were categorised further according to MSC delivery/culture techniques. These techniques included direct application, fibrin glue/gel/clot, intra-articular injection, scaffold, tissue-engineered construct, meniscus tissue, pellets/aggregates and hydrogel. Till date MSCs have been isolated from various sources in small ruminants like bone marrow, adipose tissues, amniotic fluids and characterised by flow cytometry, specific staining methods and immunocytochemistry methods. Moreover, consequent to their isolation and characterization they have differentiated into osteocytes, chondrocytes, adipocytes, neural-like cells, oocyte-like cells etc. This review focuses on the biology of MSCs, including isolation, characterization, purification, in vitro differentiation potential and goat and sheep models that can be used to test tissue-engineered constructs. © 2022, Indian Society for Sheep and Goat Production and Utilization. All rights reserved.
GROWTH KINETICS OF CAPRINE BONE MARROW-DERIVED MESENCHYMAL STEM CELLS AT DIFFERENT PASSAGES
(Indian Society for Sheep and Goat Production and Utilization, 2022) Sonam Rani; S.D. Kharche; D. Jena; S.P. Singh; Y.K. Soni; R. Nigam; R. Singh
A study was conducted on cellular growth kinetics of caprine bone marrow mesenchymal stem cells (MSCs) with the help of growth curve, population doubling time (PDT) and colony forming unit fibroblast assay (CFU-f). MSCs were cultured up to 10 passages (from P0 to P10) consequent to their isolation, purification and characterization. After employing statistical analysis it was observed that maximum cellular growth (270.00±4.33 cells/well) and minimum PDT (39.16±4.18 hr) were noticed in P3 passage followed by in passage 4 which was 263.33±5.07/well and 39.92±1.36 hr respectively. In a similar fashion maximum number of colonies were also counted in passages which depicted the maximum proliferation and differentiation ability of cells which was 74.00±3.61 in P3 followed by in P4 (66.67±3.84). The potency was calculated at the same passages on the basis of CFU-f assay which indicated that cells of P3 and P4 passages are best suited for transplantation, cryopreservation and experimental studies. © 2022, Indian Society for Sheep and Goat Production and Utilization. All rights reserved.