Browsing by Author "S.K. Yadav"
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PublicationArticle Assessment of climate change impact on different pigeonpea maturity groups in north Indian condition(Association of Agrometeorologists, 2021) M.K. Yadav; C. Patel; R.S. Singh; K.K. Singh; R. Balasubramanian; R.K. Mall; M.K. Singh; S.M. Singh; S.K. YadavThe CROPGRO-pigeonpea model embedded in DSSAT v4.7.5 was used to assess the impact of climate change on phenology and grain yield of reference genotype of different pigeonpea maturity groups. The impact of climate change delayed reproductive stages (anthesis, maturity) and decreased grain yield of reference genotype of different pigeonpea maturity groups were evident in all scenarios. Short duration genotypes (MN5, ICPL88039, Prabhat, UPAS120) showed progressively higher decrease in yield as compared to medium (Maruti, Asha, ICP7035) and long (Bahar, MAL13) duration genotypes with each successive increase in scenatio from RCP2.6 to RCP8.5 and projected year from 2010 to 2095. Anthesis was delayed 9 days in MN5 to 20 days in Bahar and maturity delayed 15 days in MN5 to 24 days in Bahar with RCP 8.5 in year 2095 in comparison to RCP2.6 in years 2010, whereas, grain yield was decreased 14% in Bahar to 66% in MN5 among genotypes of different maturity groups. © 2021, Association of Agrometeorologists. All rights reserved.PublicationArticle Assessment of climate change impact on productivity of different cereal crops in Varanasi, India(Association of Agrometeorologists, 2015) M.K. Yadav; R.S. Singh; K.K. Singh; R.K. Mall; C.B. Patel; S.K. Yadav; M.K. SinghDecision Support System for Agrotechnology Transfer (DSSAT v4.5) Cropping System Model (CSM) was used to study the impact of climate change and variability on productivity of different kharif (rice, maize, jowar and bajra) and rabi crops (wheat and barley) at Varanasi. Keeping in view the observed trends in climate variability, productivity of different kharif and rabi crops were simulated under plausible synthetic climatic scenarios of changes in temperature, solar radiation and carbon dioxide. Productivity of kharif crops viz. rice, maize, jowar and bajra and rabi crops viz. wheat, and barley decreased with an increase in temperature or a decrease in solar radiation above normal. However, productivity of different kharif and rabi crops increased under expected enhanced CO2 concentrations. Highest productivity decreased in barley crop (40.7%) of rabi season and minimum in rice crop (5%) of kharif season with an increase of 3.0 0C in temperature from normal. Whereas, maximum productivity decreased in barley crop (5.0%) of rabi season and minimum in jowar crop (1.8%) of kharif season with a decrease of 2.5 per cent in solar radiation from normal. Highest productivity increase in barley crop (58.2%) of rabi season and lowest in jowar crop (4.2%) of kharif season were simulated under expected enhanced CO2 concentration of 660 ppm. The maximum decrease in productivity of barley crop (45%) in rabi season and minimum in rice crop (7%) in kharif season were simulated when a decrease in temperature by 30C and solar radiation by 2.5 percent from normal. Highest counter-balance on productivity of rice crop (13%) in kharif season and lowest in barja crop (-23%) of kharif season were simulated when an increase in temperature by 30C from normal under expected enhanced CO2 concentration of 660 ppm. © 2015, Association of Agrometeorologists. All rights reserved.PublicationArticle Assessment of climate change impact on pulse, oilseed and vegetable crops at Varanasi, India(Association of Agrometeorologists, 2016) M.K. Yadav; R.S. Singh; K.K. Singh; R.K. Mall; Chandrabhan Patel; S.K. Yadav; M.K. SinghDecision Support System for Agrotechnology Transfer (DSSAT v4.6.1) was used to study the impact of climate change and variability on productivity of different monsoon (pigeonpea and groundnut) and winter season crops (chickpea, mustard, tomato and potato) at Varanasi. Keeping in view the observed trends in climate variability, productivity of different cash crops were simulated under plausible synthetic climatic scenarios of changes in temperature and carbon dioxide. Productivity of pigeonpea and groundnut in monsoon season and mustard, tomato and potato in winter season decreased with an increase in temperature. Productivity of different pulse, oilseed and vegetable crops increased under expected enhanced CO2 concentrations. Highest productivity decreased in pigeonpea crop (96.0%) in monsoon season and minimum in tomato crop (4.0%) in winter season with an increase of 3.0 °C in temperature above normal. Highest productivity increase in mustard crop (164.0%) in winter season and lowest in pigeonpea crop (33.0%) in monsoon season were simulated under projected enhanced CO2 concentration of 760 ppm. Highest counter-balance in productivity of mustard crop (150%) followed by tomato crop (81%) during winter season and lowest in pigeonpea crop (99%) during monsoon season were simulated when an increase in temperature by 3.0 °C above normal under projected enhanced CO2 concentration of 760 ppm. © 2016, Association of Agrometeorologists. All rights reserved.PublicationArticle CA 19-9 in various gastrointestinal malignancies in an indian population(2004) A.K. Khanna; S.K. Yadav; V.K. Dixit; G. Nath; Mohan Kumar[No abstract available]PublicationArticle Combining ability for yield and quality attributes in tomato (Lycopersicon esculentum Mill.)(Agri Bio Research Publishers, 2014) B.K. Singh; Anand K. Singh; S.K. Yadav; V. Manju Vani; Harit Kumar; B.V. RajkumarTen diverse lines of tomato were crossed with three testers in line x tester mating fashion to study combining ability effects for yield and yield traits. The parent material was sown to raise F1 during rabi season of 2009-10 and the parents along with F1 were raised in rabi season of 2010-11 at Vegetable Research Farm, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi. The analysis of variance revealed the predominance of non-additive gene action for all the traits. In respect of both gca and sea effects, the parents and hybrids differed significantly. Among the parents, Potato Leaf, Pant T-7, IC- 177371 and NOTVR- 60 were the best general combiners for improving plant height, yield per plant and other characters under study and these may be used as valuable donors in the hybridization programme for producing promising combinations. Among the crosses, RCMT-2 × VR-20, LCT-6 × VR-20 and Azad-T-5 × VR-20 were the most valuable specific combiners for yield per plant and other characters under study.PublicationArticle Comparative evaluation of locally available casing materials for quantitative and qualitative effect on two strains of agaricus bisporus (Lange)(Connect Journal, 2017) M.K. Yadav; Ram Chandra; S.K. Yadav; P.K. Dhakad; A.K. Srivastava; P.K. Dwivedi; Sushreeta NaikCasing materials are important practice for enhancement of quantitative and qualitative production of white button mushroom [Agaricus bisporus (Lange) Sing]. This investigation was done to study the effects of seven different casing materials such as Soil+ Cotton wastes (2:1), Soil+ Cotton wastes + Jute Coirpith (1:1:1), Jute Coirpit + Soil (1:1), Jute Coirpith + Soil + Sand (1:1:1), Vermi-compost+ Sand + Cotton wastes + Soil (1:1:1:1), Vermi-compost+ Saw dust+ Cotton wastes + Soil (1:1:1:1) and Vermi-compost+ Cotton wastes + Soil (1:1:1) on production and harvest quality of two strains S-1 and S-2 of A. bisporus. Result was found significantly differences in yield potential and growth behavior of mushroom due to type of casing materials. The Jute Coirpith + Soil (1:1) was gave better yield potential and growth behavior in both strains. The Soil+ Cotton wastes + Jute Coirpit (1:1:1) and Jute Coirpit + Soil + Sand (1:1:1) were good for same. Lowest yield and poor quality were obtained from Vermi-compost+ Cotton wastes + Soil (1:1:1) and Vermi-compost+ Saw dust+ Cotton wastes + Soil (1:1:1:1). Finally higher yield with fruit body quality of A. bisporus may be achieved by application of suitable casing materials. This investigation will help to farmers for selection and better utilization of locally available casing materials for obtaining higher yield of button mushroom (Agaricus bisporus).PublicationArticle Comparison of stool microscopy and serology (enzyme linked immunosorbent assay) in epidemiology of amebiasis.(1990) S.K. Yadav; A.K. Jain; V.K. Srivastava; J.P. GuptaStools from 634 individuals from Varanasi were examined for Entamoeba histolytica (EH). Serology was done in these subjects by enzyme linked immunosorbent assay (ELISA) employing filter paper technique. Stools were positive for EH in 16.9%, and serology in 15.9%. Both the tests were positive in only 5.2%. In 72.4% both the tests were negative. In 11.7% of stool positive cases, serology was negative, and in 10.7% with positive serology stool examination did not reveal EH. A majority (92.5%) of stool positive subjects had only cysts. Additional parasites were detected in 15.3%.PublicationArticle Daily variation in antioxidant enzymes lipid peroxidation in thyroid and plasma level thyroxine and triiodothyronine levels of a tropical bird Perdicula asiatica during reproductively active and inactive phases(Taylor and Francis Ltd., 2017) V.K. Verma; S.K. Yadav; C. Haldar; R.K. TripathiThe aim of this work was to study the variations in the interference of neuroendocrine pineal gland and metabolically active thyroid gland in a tropical bird, Perdicula asiatica. Maximum pineal gland activity (pineal weight and melatonin level), minimum thyroid gland activity (weight, T3/T4 and thymidine kinase activity) along with less oxidative load (MDA level, SOD, CAT and ABTS activity) were observed during reproductively inactive phase (RIP) was observed. Further, a robust and significant rhythmicity was noted in melatonin levels during RIP and RAP, but no significant rhythmicity was noted in T4/T3 level by cosinor analysis. Overall, melatonin and thyroid circadian profile suggested that melatonin might be acting as an antioxidant molecule with time of the day effect in rescuing thyroid gland from free radical load in birds. © 2016 Informa UK Limited, trading as Taylor & Francis Group.PublicationArticle Effect of glucocorticoid and melatonin on immune function of an Indian tropical bird, Perdicula asiatica: An in Vivo and in Vitro study(Biolife s.a.s., 2010) S.S. Singh; S.K. Yadav; C. HaldarInverse relationship between circulatory levels of glucocorticoids and melatonin has been reported for most of the vertebrates including human beings. We report the importance of glucocorticoid along with melatonin in avian immune regulation in an Indian tropical bird, Perdicula asiatica with in vivo and in vitro approach. Dexamethasone (30μg/bird/day) treatment of the male birds suppressed the immune activity as judged by the spleen activity (mass, anatomy), circulating total leukocyte, and lymphocyte count, blastogenic response, increased % apoptosis and cytokine (IL-2) production by splenocytes. Melatonin (25μg/100g B.wt./day) treatment increased the above-mentioned immune parameters whereas melatonin together with dexamethasone restored the suppressed immune parameters by dexamethasone to control level. In vitro melatonin (2.5 pM) supplementation to splenocyte cultures restored the dexamethasone (2 μM) suppressed splenocyte proliferation, % apoptosis and IL-2 production. Therefore, melatonin antagonized the suppressive effect of synthetic glucocorticoid on all immune parameters studied in vivo as well as in vitro. Furthermore, exogenous administration of dexamethasone and melatonin treatment altered the circulatory level of corticosterone and melatonin in an inverse manner. It is therefore, suggested that a hormonal trade-off between glucocorticoid and melatonin exists under in vivo and in vitro conditions, being involved in maintenance of the immune function of P. asiatica probably by involving cytokines i.e. IL-2 mediated pathway. Copyright © by Biolife, s.a.s.PublicationArticle Effect of organic nitrogen sources and biofertilizers on production potential and energy budgeting of rice (Oryza sativa)-based cropping systems(Indian Society of Agronomy, 2013) S.K. Yadav; Subhash Babu; Y. Singh; G.S. Yadav; Kalyan Singh; Raghavendra Singh; Harvir SinghA field experiment was conducted during 2005-06 and 2006-07 at Varanasi, Uttar Pradesh, to find out the effect of organic nitrogen sources and biofertilizers on system productivity and energy budgeting of rice (Oryza sativa L.)-based cropping system. Rice-potato [Solanum tubersoum (L.)]-onion [Allium cepa (L.)] system recorded significantly higher production efficiency (97.5 kg/ha/day), land-utilization efficiency (91.4%), economic efficiency (738/ha/day), energy input (61.08×103MJ/ha) and energy output (187.09×103MJ/ha). The lowest (39.4 × 103MJ/ ha) energy intensiveness was recorded in rice-table pea (Pisum sativum var. hortense)-cowpea [Vigna unguiculata (L.) Walp.] cropping system. However, the organic nutrition with biofertilizers (Azotobacter and phosphate-solubilizing bacteria) had the highest rice-equivalent grain yield (35.3 t/ha), production efficiency (96.7kg/ha/ day), land utilization efficiency (89.8%), economic efficiency (803 ha/day) and energy-use efficiency (3.15) of system, followed by organic nitrogen sources alone (3.0). Recommended dose of N @100% through organic nitrogen sources alone was the next best treatment resulting in higher productivity and system efficiency. Rice-potato-onion cropping system was found most productive, profitable and energy efficient with application of 100% recommended dose of nitrogen through organic sources along with biofertilizers. © 2013, Indian Society of Agronomy. All rights reserved.PublicationArticle Effect of organic nitrogen sources on yield quality and nutrient uptake of rice (Oryza sativa) under different cropping system(2013) S.K. Yadav; Yogeshwar Singh; R.P. Kumar; M.K. Yadav; Kalyan SinghA field experiment was conducted during 2005-06 and 2006-07 to study the effect of organic nitrogen sources on yield, quality and nutrient uptake of rice (Oryza sativa L.) under different cropping systems. Among the cropping system, rice-potato-onion gave the highest rice grain equivalent yield (355.7 q ha-1) and maximum net return (Rs.268656 ha-1). However the organic nitrogen nutrition (1/3FYM +1/3poultry manure + 1/3vermicompost) with bio-fertilizers (Azotobacter and PSB) had the highest rice grain equivalent yield (353.1 q ha-1) net monetary return (Rs.292454 ha-1) and benefit cost ratio (3.07). The application of organic manure alone or along with biofertilizers inoculation significantly improved the quality parameters, nutrient uptake and fertility status of soil over control. Organic nutrition treatments i.e. (1/3FYM +1/3poultry manure + 1/3vermicompost) with bio-fertilizers (Azotobacter and PSB) and also without bio-fertilizers (Azotobacter and PSB) significantly improved availability of major (nitrogen, phosphorus and potassium), and secondary (sulphur) nutrients over control under the study and the maximum gain of major (nitrogen, phosphorus, and potassium) and secondary (sulphur) nutrients was associated with the application of organic matter (1/3FYM +1/3poultry manure + 1/3 vermicompost) along with bio-fertilizers (Azotobacter and PSB).PublicationArticle Effect of organic nitrogen sources on yield, nutrient uptake and soil health under rice (Oryza sativa) based cropping sequence(2013) S.K. Yadav; Yogeshwar Singh; M.K. Yadav; Subhash Babu; Kalyan SinghA two year of field experimentation was carried out at Varanasi from 2005-07 to study the effect of organic nitrogen sources on yield, nutrient uptake and soil health under rice (Oryza sativa) based cropping sequence. The soil was sandy clay loam in the texture with pH 7.4, moderately fertile, being low in organic carbon (0.50%), available nitrogen (185.5 kg/ha). The experiment on rice based cropping sequences with 3 organic N nutrition treatments was laid out in split plot design with three replications. Seven rice based cropping sequences [rice-potato-onion; rice-green pea-onion; rice-potatocowpea (green pod); rice-green pea-cowpea (green pod); rice-rajmash (green pod)-onion; rice-rajmash (green pod)-cowpea (green pod) and rice-maize (green cob)-cowpea (vegetable)] were assigned to main plots and three treatments comprising nitrogen application (control; organic manure; organic manure + bio-fertilizer) were allocated to sub plots. The system productivity was the highest (355.73 q/ha) with rice-potato-onion cropping sequence under investigation. Among the manurial treatment, organic nitrogen nutrition with biofertilizers had the highest rice grain equivalent yield (353.08 q/ha) and net monetary return (292 454). The application of organic manure alone or along with biofertilizers inoculation significantly improved the N, P, K and S uptake by cropping sequence over control. However maximum improvement in soil health related to soil organic carbon, available nutrient status, soil micro-organism population were observed in organic nitrogen sources alone or along with biofertilizers.PublicationArticle Effect of organic nutrient sources on yield, nutrient uptake and soil biological properties of rice (Oryza sativa)-based cropping sequence(2013) S.K. Yadav; Subhash Babu; Yogeshwar Singh; M.K. Yadav; G.S. Yadav; Suresh Pal; Raghavendra Singh; Kalyan SinghA field experiment was carried out at Varanasi for two years (2005-2007) to study the effect of organic nutrient sources on yield, nutrient uptake and soil properties of rice (Oryza sativa L.) based cropping sequence. Seven rice-based cropping sequences viz. rice-potato (Solanum tuberosum L.)-onion (Allium cepa L); rice-green pea (Pisum sativum var. hortense)-onion; rice-potato-cowpea (Vigna unguiculata subsp. sesquipedalis); rice-green pea-cowpea; rice-rajmash (Phaseolus vulgaris L.)-onion; rice-rajmash-cowpea and rice-maize (Zea mays L.)-cowpea were assigned to main plots and three treatments of nitrogen application viz. control; organic manure; organic manure + biofertilizer were allotted to sub plots. Highest rice equivalent yield (35.57 t/ha), monetary net returns (268.7×103/ha), production efficiency (96.81 and 98.11 kg/ha/day) and irrigation efficiency (504.8 and 511.6 kg/ha-cm) were found under rice-potato-onion cropping sequence. Among the organic treatments, the highest rice equivalent yield (35.3 t/ha), production efficiency (95.7 and 97.8 kg/ha/day), irrigation efficiency (515.7 and 527.4 kg/ha-cm) and soil biological indictors like dehydrogenase activities, urease activities, soil microbial biomass carbon and soil microbial biomass nitrogen were obtained under organic nutrient management combined with biofertilizers.PublicationArticle Gastro-esophageal reflux disease (GERD): an appraisal of different tests for diagnosis.(1990) J.P. Gupta; A. Kumar; A.K. Jain; S.K. Yadav; M. Kumar; A.K. AgrawalFifty symptomatic patients with GERD, 20 each of non ulcer dyspepsia (NUD) & duodenal ulcer (DU) and 10 healthy controls were subjected to various tests employed for diagnosis of GERD. Among these endoscopy and histology had highest sensitivities (92% & 91% respectively) followed by Bernstein's test (overall 88%; early positivity 72%) and oesophagography (70%). The specificities of various tests were: endoscopy (86%), histology (82%) and Bernstein's test (overall 80%; early positive 94%). The false positivity was mainly in DU subjects where majority (greater than 84%) had two or more of these tests offitive. Any two of the three tests (endoscopy, histology & Bernstein's test) in combination had a sensitivity of 80-91% and a specificity of 90-92%. Our observations suggest that these tests, particularly in combination, are useful in establishing the diagnosis of GERD and that subclinical oesophagitis in DU might be responsible for the false positivity of these tests.PublicationArticle Irritable bowel syndrome: Therapeutic evaluation of indigenous drugs(1989) S.K. Yadav; A.K. Jain; S.N. Tripathi; J.P. Gupta[No abstract available]PublicationArticle Mn(II) assisted synthesis of N-phenyl-5-(pyridin-3-yl)-1,3,4-oxadiazol-2-amine and evaluation of its antiproliferative activity(Elsevier B.V., 2023) Shubham Jaiswal; Shivendra Kumar Pandey; Tarun Minocha; S. Chandra; M.K. Bharty; S.K. Yadav; D. Kushwaha; R.J. ButcherThe global burden of cervical cancer is continuously increasing day by day affecting the world population. Wide arrays of agents are prevalent for the treatment of this chronic disease but the survival rates are still limited. The titled compound N-phenyl-5-(pyridin-3-yl)-1,3,4-oxadiazol-2-amine (Pypao) was prepared through the cyclization of nicotinoyl-N-phenyl hydrazine carbothioamide catalyzed by manganese (II) acetate. Uv-Vis, IR, NMR, elemental analyses, and single-crystal X-ray data were utilized for the description of the compound Pypao. The crystal structure of Pypao is stabilized via intermolecular hydrogen bonding interactions, which is further confirmed with Hirshfeld surface analysis. The DFT methods were also used to optimize the Pypao and obtained bond parameters have been found coherent with the experimental data. FMO and TD-DFT studies are also performed to gain insights into the electronic behavior of Pypao. Furthermore, the binding potential of Pypao to different oncoproteins of HPV namely E6, E6AP, and E7 have been examined through a molecular docking study. The in-vitro anticancer activity of Pypao has been investigated against SiHa cells. The anticancer potential of Pypao has been evaluated through various assays including MTT, DAPI stating, AO/PI staining, etc. Both time and dose-dependent activity of Pypao have been investigated. © 2023 Elsevier B.V.PublicationArticle Neuroticism in patients with irritable bowel syndrome.(1990) S.K. Yadav; A.K. Jain; P.B. Bahre; J.P. GuptaEmploying a Hindi adaptation of the Middlesex Hospital Questionnaire (MHQ), neuroticism level was assessed in 133 subjects with irritable bowel syndrome and compared with that in patients with organic bowel disease (33), healthy population (140) and known neurotics (110). Compared with healthy controls and patients with organic bowel disease, significantly higher scores were found among irritable bowel syndrome patients in all the subscales except obsession. The total of scores was midway between those of the normal population and known neurotics. Neurotic disorder was detected in 51% of irritable bowel syndrome patients compared to 24% of organic bowel disease subjects (P less than 0.05) and 7.8% of the healthy population. Cut-off points, calculated as mean + 2 SD of scores in the healthy population, were found to be reliable as shown by clinical interview.PublicationArticle Production potential and nutrient uptake of wheat (Triticum aestivum) as affected by organic sources of nutrients and micronutrients(Indian Council of Agricultural Research, 2014) M.K. Yadav; R.K. Singh; S.K. YadavA field experiment was conducted during rabi season of 2005-06 and 2006-07 to study the effect of micronutrients and FYM on growth, yield and nutrient uptake by wheat (Triticum aestivum L.) in Gangetic alluvial soil of Uttar Pradesh. The experiment was laid out in split plot design with three replications. Wheat crop recorded significantly higher value of growth, yield attributes (effective tillers/m2, spike length, grains/spike and 1 000 grain weight), yields, benefit: cost ratio and nutrient accumulation under integrated source of nutrients than inorganic fertilizer alone. Maximum grain yield (4.45 tonnes/ha) was recorded with Zn @ 5 kg/ha + FYM @ 10 tonnes/ha + RFD, which was about 36.01% more than the grain yield obtained from application of 2.5 kg Zn /ha + recommended fertilizer dose. There was significant improvement in yield attributes, yields and nutrient uptake due to foliar treatment of micronutrients (Fe and Mn).PublicationArticle Profitability of high value crops with organic nitrogen sources under rice (Oryza sativa) based cropping sequence(Indian Council of Agricultural Research, 2014) S.K. Yadav; Subhash Babu; M.K. Yadav; Yogeshwar Singh; Kalyan SinghA field experiment was conducted during 2005-06 and 2006-07 at Campus Research Farm, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi to identify a suitable high value cropping sequence with organic nitrogen sources under rice (Oryza sativa L.) based cropping system. The experiment was laid out in split plot design with three replications. Seven rice based cropping sequences, viz. rice-potato-onion; rice-green pea-onion; rice-potatocowpea (green pod); rice-green pea-cowpea; rice-rajmash (green pod)-onion; rice-rajmash-cowpea and rice-maize (green cob)-cowpea were assigned to main plots and three organic treatments (control; 100% RDN through organic manure along with biofertilizers and 100% RDN through organic manure alone) were allocated to sub plots. Among the cropping sequences, rice-potato-onion gave the highest rice grain equivalent yield (35.57 tonnes/ha), maximum net return (268 656/ha), profitability (738/ha/day) and labour employment generation (469 man days/ha/year). However rice yield and soil fertility status was not significantly affected by cropping sequences. Application of 100% recommended dose of through organic manure along with biofertilizers (Azotobacter and PSB) had the highest rice equivalent grain yield (35.31 tonnes/ha), production efficiency (96.74 kg/ha/day), net monetary return (292 454/ ha), profitability (803/ha/day) and labour employment generation (419 man days/ha/year). Inclusion of pulses in sequence with proved superior due to its viable favorable effect on soil fertility. Thus organic nitrogen nutrition with biofertilizers had the highest rice equivalent grain yield, production efficiency, net monetary return and profitability.PublicationArticle Reciprocal interaction between melatonin receptors (Mel1a, Mel1b, and Mel1c) and androgen receptor (AR) expression in immunoregulation of a seasonally breeding bird, Perdicula asiatica: Role of photoperiod(2013) S.K. Yadav; C. HaldarLight is the major environmental stimulus affecting behaviour and physiology of avian species. Our study elaborates the photoperiodic regulation of melatonin (Mel1a, Mel1b, and Mel1c) and androgen receptor (AR) to elucidate its reciprocal interaction in regulation of general immunity in tropical wild bird, Perdicula asiatica. Effect of different photoperiodic exposures such as continuous light (LL), continuous dark (DD), long days (LD; 16 h light/day), short days (SD; 10 h light/day) and normal day length (NDL) was accessed both on cellular and humoral immune parameters like per cent stimulation ratio (%SR), total leukocyte count (TLC), leukocyte count (LC), plasma interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α), spleen and gonad weight, plasma melatonin, and testosterone level as well as their receptor expression on spleen and testis. Expression of melatonin receptor, Mel1a and Mel1b in spleen was high in SD experiencing bird as compared to LD birds. In all photoperiodic groups, AR expression was upregulated in spleen. In addition, our reverse transcription polymerase chain reaction (RT-PCR) results support differentially localized mRNA Mel1b and Mel1c expression in spleen and testis. In sum, photoperiodically modulated level of melatonin via reciprocal regulation of Mel1a, Mel1b, and Mel1c, and AR in spleen as well as in testis modulates immunity, suggesting a compensatory mechanism between reproduction and immunity in a seasonally breeding bird, P. asiatica. © 2013 Elsevier B.V. All rights reserved.
