Browsing by Author "S.P. Agarwal"

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Can dual staining with p16 and Ki67 be biomarkers of epithelial dysplasia in oral lesions?
(Wolters Kluwer Medknow Publications, 2022) Anju Bharti; Sumaira Qayoom; Riddhi Jaiswal; Preeti Agarwal; R.K. Singh; S.P. Agarwal; Shalini Bhalla; Annu Makker; Madhu Mati Goel
Background: Oral carcinogenesis is a multistage process with epithelial dysplasia as a premalignant condition. There is a significant inter-observer variation in diagnosing and grading the oral epithelial dysplasia. As human papillomavirus (HPV) is believed to have à strong relationship with oral carcinogenesis, using P16 as a biomarker may help in identifying the cells which may be undergoing the malignant transformation. However, due to the low specificity of P16, dual staining test P16 INK4 /Ki67 might be a better promising marker for identifying the transformed cells. This study was designed to evaluate the dual expression of P16 and Ki67 as a promising biomarker for dysplasia and their correlation with clinicopathological factors. Materials and Methods: Immunohistochemical analysis for p16 and ki67 was performed on 30 premalignant oral lesions and 36 oral squamous cell carcinoma (OSCC) by dual staining using the CINtec PLUS kit. Results: CINtec positivity was observed only in leukoplakia with dysplasia (46.7%) and squamous cell carcinoma (25%). None of the cases of leukoplakia without dysplasia or oral submucosal fibrosis stained positive for CINtec plus staining. In leukoplakia with dysplasia, there was no significant association with any of the clinicopathological parameters studied. In OSCC cases, alcohol intake showed statistically significant association with CINtec positivity. Conclusion: P16 INK4 /Ki67 assessment by dual staining is a promising biomarker for identifying dysplasia in cases with diagnostic dilemmas. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Clinical and aetiological study of childhood mental retardation
(1974) G. Subrahmanyam; K.N. Agarwal; S.P. Agarwal; A. Jaiswal
[No abstract available]
Dermatoglyphics in vitiligo
(1975) R.G. Sahasrabuddhe; G. Singh; S.P. Agarwal
A statistical comparison of dermatoglyphic features of hands was made on 50 children with vitiligo and 100 without as controls. Vitiligo males were different from normals in possessing decreased frequency of whorls and increased incidence of radial loops. Decreased frequency of whorls was significant on both hands. Digit wise distribution of patterns revealed increased frequency of radial loops on the second digit and of arches on the third digit. The ulnar loops were increased and whorls were decreased on the fifth digit. Features in vitiligo females were an increased frequency of arches (total patterns), decreased ulnar loops on the third digit and a significant reduction of thenar/11 patterns. Ulnar loops (total patterns) were reduced significantly on the right hand.