Browsing by Author "Safiya Ayesha"

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Correction: Intervention of Phytochemicals During Endometriosis and Their Conceivable Mechanisms (Revista Brasileira de Farmacognosia, (2023), 33, 6, (1126-1140), 10.1007/s43450-023-00426-2)
(Springer Science and Business Media Deutschland GmbH, 2023) Safiya Ayesha; Alka Sharma; Jayhind Kumar Chauhan; Vivek Pandey; Garima Tripathi; Pawan K. Dubey; Anima Tripathi
The graphical abstract in the original online version of this article was incorrect. It has been corrected. © 2023, The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia.
Intervention of Phytochemicals During Endometriosis and Their Conceivable Mechanisms
(Springer Science and Business Media Deutschland GmbH, 2023) Safiya Ayesha; Alka Sharma; Jayhind Kumar Chauhan; Vivek Pandey; Garima Tripathi; Pawan K. Dubey; Anima Tripathi
Endometriosis is a chronic and complex endocrine disorder that affects women of reproductive age. This syndrome is benign and is characterized by a combination of ovarian dysfunction and estrogen dependency symptoms, as well as pain and infertility. The most commonly stated symptoms include dysmenorrhea, significant dyspareunia, dyschezia, and dysuria. It is a severe public health issue. The prevalence rate is quite high and continues to rise on a daily basis. Starting with its diagnosis, pathophysiology, repercussions, and treatment options, there are numerous areas of disagreement. This study aims to provide an overview of the development of endometriosis diagnosis, symptoms, risk factors, etiology, medicinal plants, phytochemicals, and treatment, with a focus on research and the creation of novel therapeutic strategies. We conclude by making predictions and recommendations for the future of endometriosis-related research and prospective therapy approaches. Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia.
Shatavarin-IV rescues the Di (2-ethylhexyl) phthalate (DEHP) induced oxidative stress in rat granulosa cells in vitro
(Elsevier Inc., 2024) Vivek Pandey; Alka Sharma; Sonal Tiwari; Yashvant Patel; Jayhind Kumar Chauhan; Safiya Ayesha; Alakh N. Sahu; Rashmi Gupta; Anima Tripathi; Pawan K. Dubey
Studies provide notable evidence that oxidative stress (OS) mediated reactive oxygen species (ROS) disturb reproductive health. We have shown in our previous publication that exposure of Di-(2-ethylhexyl) phthalate (DEHP), induces OS mediated ROS generation which inhibits steroid synthesis. In the present study, we demonstrated the ameliorative/protective effects of one of the steroidal saponins, i.e., Shatavarin-IV, isolated from the roots of Asparagus racemosus against DEHP induced OS in rat granulosa cells. Granulosa cells were exposed with DEHP alone (400 μM), Shatavarin-IV alone (8 μg/ml), and a combination of DEHP + Shatavarin-IV (400 μM + 8 μg/ml) in vitro for 24 hrs. Intracellular ROS, OS/hypoxia, mitochondrial membrane potential, steroid-responsive genes expression were analyzed. The results revealed that the effective dose of DEHP (400 µg) significantly increased OS compared to the control by increasing ROS levels, mitochondrial membrane potential, and β-galactosidase activity with a higher level of apoptotic genes (Bax, Caspase-3) expression at mRNA level. Further, DEHP significantly (p < 0.05) reduced mRNA expression of steroidogenic responsive genes (StAR, CYP17A1, and CYP19A1) in granulosa cells treated with above combination compared to control. Interestingly, co-treatment of DEHP + Shatavarin-IV significantly suppressed the DEHP induced OS, ROS, β-galactosidase levels and enhanced steroidogeneic and apoptotic gene expression activities, which suggests that Shatavarin-IV rescued DEHP-induced changes that may useful for the prevention of DEHP- induced reproductive toxicity. © 2024 Elsevier Inc.