Browsing by Author "Sana Fatma"

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A reduced graphene oxide ceramic electrode modified with one MoNomer doubly imprinted acryloylated tetraamine cobalt phthalocyanine polymer for the simultaneous analysis of anticancerous drugs
(Elsevier B.V., 2019) Sana Fatma; Bhim Bali Prasad; Kislay Singh; Richa Singh; Swadha Jaiswal
The present work illustrates a novel technique for the development of One MoNomer dual imprinted polymer, utilizing acryloylated tetraamine cobalt phthalocyanine as a tetrafunctional monomer, on the surface of a reduced graphene oxide ceramic electrode. This electrode was used for the simultaneous analysis of a mixture of anticancerous drugs, chlorambucil and dacarbazine, at ultra-trace level in real samples. The acryloylated tetraamine cobalt phthalocyanine was typically selected in this work for polymerization because it served both as a monomer and a crosslinker, with higher electroconductivity. The detection sensitivity of the measurement was realized to be 0.041 ng mL−1 and 0.017 ng mL−1 for chlorambucil and dacarbazine, respectively in the aqueous environment. The complex matrices of blood plasma, urine and pharmaceutics were examined which yielded the validated results without any effects of matrix complications including cross-reactivity, and false-positives. The therapeutic ranges of the test analyte(s) (chlorambucil 0.159–28.524 ng mL−1, dacarbazine 0.063–37.286 ng mL−1) were realized to be larger demonstrating perfect linearity (R2 = 0.99) with the improved voltammetric response. This work merits special significance to decide the adequate supplementation of drug(s) in combined therapy for cancer treatment. © 2018 Elsevier B.V.
A spectroscopic and molecular docking approach on the binding of tinzaparin sodium with human serum albumin
(Elsevier B.V., 2017) Saleh M.S. Abdullah; Sana Fatma; Gulam Rabbani; Jalaluddin M. Ashraf
Protein bound toxins are poorly removed by conventional extracorporeal therapies. Venous thromboembolism (VTE) is a major cause of morbidity and mortality in patients with cancer. The interaction between tinzaparin, an inhibitor of angiotensin converting enzyme and human serum albumin, a principal plasma protein in the liver has been investigated in vitro under a simulated physiological condition by UV–vis spectrophotometry and fluorescence spectrometry. The intrinsic fluorescence intensity of human serum albumin was strongly quenched by tinzaparin (TP). The binding constants and binding stoichiometry can be calculated from the data obtained from fluorescence quenching experiments. The negative value of ΔG° reveals that the binding process is a spontaneous process. Thermodynamic analysis shows that the HSA-TP complex formation occurs via hydrogen bonds, hydrophobic interactions and undergoes slight structural changes as evident by far-UV CD. It indicated that the hydrophobic interactions play a main role in the binding of TP to human serum albumin. In addition, the distance between TP (acceptor) and tryptophan residues of human serum albumin (donor) was estimated to be 2.21 nm according to the Förster's resonance energy transfer theory. For the deeper understanding of the interaction, thermodynamic, and molecular docking studies were performed as well. Our docking results suggest that TP forms stable complex with HSA (Kb ∼ 104) and its primary binding site is located in subdomain IIA (Sudlow Site I). The results obtained herein will be of biological significance in pharmacology and clinical medicine. © 2016 Elsevier B.V.
Biomimetic Polymer-Based Electrochemical Sensor Using Methyl Blue-Adsorbed Reduced Graphene Oxide and Functionalized Multiwalled Carbon Nanotubes for Trace Sensing of Cyanocobalamin
(American Chemical Society, 2018) Richa Singh; Swadha Jaiswal; Kislay Singh; Sana Fatma; Bhim Bali Prasad
The paper reports a three-dimensional cyanocobalamin (Cbl)-selective biomimetic imprinted polymer. For this, a methyl blue-adsorbed reduced graphene oxide (rGO) and functionalized multiwalled carbon nanotubes (f-MWCNTs) composite, duly functionalized with acryloylurea, was used. This exhibited higher electroconductivity and larger surface area in comparison to either pristine carbon nanotubes or graphene. It is the first synthetic biomimetic polymer of Cbl, grown on the surface of a pencil graphite electrode. In this work, the adsorption of methyl blue over the rGO sheets improved its solubility, conductivity, and self-assembly properties. Such assemblies of rGO sheets and f-MWCNTs provide enhanced kinetics and conductivity to the entire architectural design of a molecularly imprinted polymer. The ultratrace detection of Cbl was feasible by differential-pulse voltammetric transduction. This sensor exhibited high sensibility and selectivity for Cbl detection, especially in real samples. © 2018 American Chemical Society.
Effect of Different Storage Conditions on Analytical and Sensory Quality of Thermally Processed, Milk-Based Germinated Foxtail Millet Porridge
(Blackwell Publishing Inc., 2018) Nitya Sharma; Tanweer Alam; S.K. Goyal; Sana Fatma; Sheetaal Pathania; Keshavan Niranajan
Abstract: Foxtail millet porridge was prepared using germinated grains and milk and was evaluated for its storage stability after thermal processing at ultra-high temperatures (UHT) of 142 °C for 5 s and retort processing temperatures of 121.5 °C for 15 min. Various physical, chemical, and microbial changes of the porridge were studied for a storage period of 180 days at 25 ± 1 °C. Using consumer perception and survival analysis, the predicted shelf life of the UHT treated and retort processed foxtail millet porridge samples stored at 25 ± 1 °C was found to be 186 ± 9 days and 245 ± 15 days, respectively. Also, data from consumer liking, profiling, physical, chemical, and microbial parameters showed significant changes (P < 0.05) in the thermally treated packaged porridge samples over time. As the consumer overall acceptability decreased, the detection of positive attributes (thick and uniformly colored texture and appearance; grainy mouth texture; caramel taste and aroma) in the porridge decreased, while the detection of negative attributes (uneven, decolored, and curdled texture and appearance; sticky mouth texture; cooked, sour and off smell; cooked, sour and off taste) increased. The present study could establish a significant difference (P < 0.05) in the storage induced properties of UHT and retort processed porridge samples. The analytical evaluation of foxtail millet porridge found that UHT treated porridge was better in quality, but consumers preferred retort processed porridge. Practical Application: The quality and sensory attributes, evaluated for UHT treated and retort processed porridge samples during the storage period of 180 days, were found to be contradictory. Based on the results of CATA sensory analysis, the shelf life of UHT treated and retort processed porridge samples was predicted to be more than 6 months. Therefore, both UHT treatment and retort processing can be effectively applied to prepare a ready to eat milk based porridge using germinated foxtail millet grains. © 2018 Institute of Food Technologists®
Effect of Germination on the Functional and Moisture Sorption Properties of High–Pressure-Processed Foxtail Millet Grain Flour
(Springer New York LLC, 2018) Nitya Sharma; S.K. Goyal; Tanweer Alam; Sana Fatma; Keshavan Niranjan
Foxtail millet is one of the commonly cultivated, nutritionally competitive source of protein, fibre, phytochemicals and other micronutrients, as compared to major cereals like wheat and rice. Considering the potential of these grains, the high pressure processed flours of germinated (GFMF) and non-germinated foxtail millet (NGFMF) grains were studied for its functional, moisture sorption and thermodynamic properties. Germination and high-pressure processing of foxtail millet grains significantly improved the functional properties of the flour. Apart from this, the moisture sorption isotherms of both the flours were determined at 10, 25 and 40 °C and the sorption data was fitted to Guggenheim-Anderson-De Boer (GAB) sorption model. The monolayer moisture content for NGFMF and GFMF ranged between 3.235–2.364 and 2.987–2.063 g g−1, respectively. The isosteric heat of sorption ranged between − 76.35 to − 38.23 kJ mol−1 for NGFMF and 172.55 to − 34.02 kJ mol−1 for GFMF at a moisture range of 0 to 36%, whereas, the integral entropy of sorption for NGFMF ranged between − 0.404 and − 0.120 kJ mol−1 K−1 and for GFMF between − 0.667 and − 0.383 kJ mol−1 K−1. Along with the validation of the compensation theory, the values of spreading pressures lied in the range of 0–0.078 J m−2 for NGFMF and 0– 0.124 J m−2 for GFMF, while, the glass transition temperatures ranged between 82.25 and 28.67 °C for NGFMF and from 51.11 to 11.83 °C for GFMF at all three temperatures. © 2017, Springer Science+Business Media, LLC.
Effect of high pressure soaking on water absorption, gelatinization, and biochemical properties of germinated and non-germinated foxtail millet grains
(Academic Press, 2018) Nitya Sharma; S.K. Goyal; Tanweer Alam; Sana Fatma; Araya Chaoruangrit; Keshavan Niranjan
Foxtail millet is one of the few crops that can thrive under relatively few agricultural inputs and have valuable amount of nutritional components. Therefore, processing of foxtail millet for value addition to various food products can significantly help in economic development as well as enhancing food and nutritional security. This study deals with the effect of high pressure soaking on water uptake, gelatinization characteristics, and nutritional and anti-nutritional properties of foxtail millet grains. The results demonstrated that high pressure soaking of germinated foxtail millet grains significantly increased the water uptake, thereby increasing the degree of starch gelatinization of the flour to attain a maximum value of 64.93%. The effective diffusion coefficient of water was found to increase with increasing pressures and temperatures, reaching maximum value of 6.77 × 10−9 m2s−1 for germinated foxtail millet grains treated at 200 MPa and 60 °C. For germinated foxtail millet grain flour, the total phenolic content and antioxidant activity (FRAP assay) improved significantly, although the protein content did not vary significantly. Further, the levels of anti-nutrients (phytic acid and tannin) decreased with high pressure soaking, which conclusively establishes that the quality of foxtail millet grains and its flour can be improved by using high pressure soaking. © 2018 Elsevier Ltd
Electrochemical sensing of ultra trace copper(II) by alga-OMNiIIP modified pencil graphite electrode
(Elsevier, 2016) Bhim Bali Prasad; Sana Fatma
One MoNomer Ion Imprinted Polymer (OMNiIIP) development is an attractive concept which typically uses a crosslinking monomer that can act both as a functional monomer and crosslinker, simultaneously. This technique is meant for enhancing binding and selective characteristics of polymer vis-a-vis traditionally formulated ethylene glycol methacrylate and functional monomer based imprinted polymers. The present work is aimed to evaluate Cu (II) ions using OMNiIIP developed from an acryloylated acidified biomass of blue green algae, Aulosira sp., which is efficient to mitigate non-specific adsorptions, especially biosorptive metal ions accumulation. The alga-OMNiIIP modified pencil graphite electrode was used for differential pulse anodic voltammetric analysis of copper (II) with detection limit upto 0.0018 ng mL-1 (3σ). Algal stains in monomeric unit of OMNiIIP chain in this work have not only inculcated its electroconducting activity, but also facilitated metal ion imprinting, exploiting their ligating carboxylate groups present in higher ratio. © 2016 Elsevier B.V. All rights reserved.
Electrochemical simultaneous analysis of dopamine and epinephrine using double imprinted One MoNomer acryloylated graphene oxide-carbon black composite polymer
(Elsevier Ltd, 2019) Sana Fatma; Bhim Bali Prasad; Swadha Jaiswal; Richa Singh; Kislay Singh
A novel One MoNomer dual imprinted graphene oxide/carbon black composite polymer was developed applying ‘surface-grafting from’ approach on the screen printed carbon electrode for the electrochemical sensing of dopamine and epinephrine. Acryloylated-graphene oxide/carbon black was synthesized for the first time. This served both as a crosslinker and monomer leading to the fast electron transfer from the redox centre to the electrode. The oxidation peak potentials of both the targets were found separated by 200 mV which enabled their simultaneous analysis in real world samples, without any cross reactivity, interferences, and false-positives. The detection limits realized by the proposed sensor, under optimized analytical conditions, were found to be as low as 0.028, 0.028,0.061 and 0.029 ng mL −1 for dopamine and 0.017, 0.018, 0.019 and 0.020 ng mL −1 for epinephrine (S/N = 3) in aqueous, blood serum, urine and pharmaceutical samples. Such sensor could be considered suitable for the primitive diagnosis of several chronic diseases, manifested at ultra-trace level. © 2019
Enantioselective analysis of D- and L- Serine on a layer-by-layer imprinted electrochemical sensor
(Elsevier Ltd, 2019) Swadha Jaiswal; Richa Singh; Kislay Singh; Sana Fatma; Bhim Bali Prasad
The present work describes a new, simple, and easy method of generating acrylamide functionalised reduced graphene oxide-fullerene layer-by-layer assembled dual imprinted polymers to quantify D- and L-Serine at ultra trace level in aqueous and real samples. Herein, the pencil graphite electrode was initially spin coated with D-Serine imprinted acrylamide functionalized reduced graphene oxide. After 10 min thermal treatment (50 °C), this electrode was again modified with L-Serine imprinted acrylamide functionalized fullerene molecules. This bilayer assembly was finally made thermally stable by 60 °C exposure for 3 h. The proposed sensor showed better electronic properties with an improved synergism. We have compared this modified electrode with other modified pencil graphite electrodes like single layered acrylamide functionalised reduced graphene oxide or fullerene, single layered acrylamide functionalised reduced graphene oxide-fullerene composite and double layered acrylamide functionalised reduced graphene oxide or fullerene molecules, which yielded very inferior sensitivity due to possible agglomeration and decreased synergism. The chosen system demonstrated a very good analytical figures of merit with differential pulse anodic stripping voltammetry and cyclic voltammetry transduction, showing lower limits of detection (0.24 ng mL −1 , S/N = 3) for both isomers. The proposed sensor assures practical applications as disease biomarker, manifesting several diseases at very ultra-trace level. © 2018 Elsevier B.V.
Inhibiting Effect of Zinc Oxide Nanoparticles on Advanced Glycation Products and Oxidative Modifications: a Potential Tool to Counteract Oxidative Stress in Neurodegenerative Diseases
(Humana Press Inc., 2018) Jalaluddin M. Ashraf; Mohammad Azam Ansari; Sana Fatma; Saleh M. S. Abdullah; Johar Iqbal; Aymen Madkhali; Al Hassan Hamali; Saheem Ahmad; Ahmed Jerah; Valentina Echeverria; George E. Barreto; Ghulam Md Ashraf
Advanced glycation end products (AGEs) are implicated in several central nervous system (CNS) pathologies including Alzheimer and Parkinson’s diseases. In the face-off of AGE menace, we have attempted to investigate the zinc oxide nanoparticle (ZnONP) role in inhibition of AGE formation. Synthesized ZnONPs were used to investigate the inhibitory effects on AGE formation. The inhibitory effects of ZnONPs on AGE formation were determined by biophysical immunological and biochemical techniques. The results showed that ZnONP is a potential anti-glycating agent inhibiting AGE formation as well as protecting the protein structure from change. Therefore, our findings suggest ZnONPs may be used as a therapeutic in resolving the AGE role in CNS-related complications. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
MODERN APPROACHES IN FOOD PACKAGING WASTE MANAGEMENT
(Apple Academic Press, 2009) Nitya Sharma; Aastha Bhardwaj; Sukirti Joshi; J.K. Sahu; Sana Fatma
Due to the current state of eco-pollution produced by plastic packaging wastes that are usually found contaminating soils and fresh water, it is critical to develop a long-term solution, particularly for the food industry. As previously said, packaging wastes from foods makes about two-thirds of total volume of the packaging wastes, which is driven by a variety of factors such as rising economies, new products, public perception changes, consumerism, increases in income, population growth, and so on. Therefore, methods of treating and processing of food packaging wastes must be relooked to understand the environmental, economic, and social ramifications involved with food packaging waste management. Source reduction, recycling, composting, burning, and landfilling are all part of the traditional integrated waste management method. Although just by altering the process design of these conventional food packaging waste treatment methods, yet various modern green waste management approaches are now being developed. Therefore, this chapter presents system of waste generation in the sector of food and then provides an insight on the conventional as well as modern food packaging waste management approaches, finally giving a detailed comparison thereof. Additionally, the goal of this chapter would be to widen understanding of packaging waste treatment techniques in the food sector. © 2024 by Apple Academic Press, Inc.
One MoNomer doubly imprinted dendrimer nanofilm modified pencil graphite electrode for simultaneous electrochemical determination of norepinephrine and uric acid
(Elsevier Ltd, 2017) Bhim Bali Prasad; Sana Fatma
One MoNomer dual imprinted hyperbranched polymer (dendrimer) with dispersed gold nanoparticle-functionalized multiwalled carbon nanotubes composite was used for the growth of a nanometer thin film applying ‘surface-grafting from’ approach on the pencil graphite electrode. The idea of combining dual template imprinting and One MoNomer molecular imprinting for the development of a dendritic nano-film, with homogenously generated molecular cavities (dendritic-boxes), is novel for the simultaneously better ingress-egress of pair of analytes (norepinephrine and uric acid). The differential pulse anodic stripping voltammetric peak potentials for both the analytes were found to be well apart approximately by 200 mV. This enabled simultaneous analysis, one in the presence of other, without any cross reactivity, interferences, and false-positives. The detection limits realized by the proposed sensor, under optimized analytical conditions, were found to be as low as 0.62 ng mL−1 for norepinephrine and 0.43 ng mL−1 for uric acid (S/N = 3) in aqueous, biological and pharmaceutical samples. Such stringent limits could be considered suitable for the primitive diagnosis of several chronic diseases, in clinical settings. © 2017 Elsevier Ltd
One-by-one imprinting in two eccentric layers of hollow core-shells: Sequential electroanalysis of anti-HIV drugs
(Elsevier Ltd, 2018) Kislay Singh; Swadha Jaiswal; Richa Singh; Sana Fatma; Bhim Bali Prasad
Double layered one-by-one imprinted hollow core-shells@ pencil graphite electrode was fabricated for sequential sensing of anti-HIV drugs. For this, two eccentric layers were developed on the surface of vinylated silica nanospheres to obtain double layered one-by-one imprinted solid core-shells. This yielded hollow core-shells on treatment with hydrofluoric acid. The modified hollow core-shells (single layered dual imprinted) evolved competitive diffusion of probe/analyte molecules. However, the corresponding double layered one-by-one imprinted hollow core-shells (outer layer imprinted with Zidovudine, and inner layer with Lamivudine) were found relatively better owing to their bilateral diffusions into molecular cavities, without any competition. The entire work is based on differential pulse anodic stripping voltammetry at double layered one-by-one imprinted hollow core-shells. This resulted in indirect detection of electro inactive targets with limits of detection as low as 0.91 and 0.12 (aqueous sample), 0.94 and 0.13 (blood serum), and 0.99 and 0.20 ng mL-1 (pharmaceutics) for lamivudine and zidovudine, respectively in anti-HIV drug combination. © 2018 Elsevier B.V.
Processing induced changes on coarse cereals (majorly millets) derived antioxidant compounds-a review
(Functional Food Institute, 2022) Dipesh Aggarwal; Aastha Bhardwaj; Anupreet Kaur Sobti; Sana Fatma; Nitya Sharma; Vasudha Bansal
Coarse cereals also known as nutricereals contain several bioactive components that provide many health-promoting and disease-preventing properties. This paper presents a review of the effect of processing on the various antioxidant compounds present in coarse cereals. Polyphenols, phenolic compounds, flavonoids, tannins, avenanthramides, vitamins, and phytoestrogens are the major categories that contribute to the antioxidant properties of coarse cereals. As per the literature, processing technologies like fermentation, boiling, malting, hydrolysis, soaking and germination, heat treatment, microwaving and extrusion, etc, have a significant effect on these antioxidant compounds present in coarse cereals. Coarse cereals and their processed products could be of potential benefit to human health, but extensive research is required to optimize the dietary recommendation for realizing these health benefits. © FFC 2022.
Ultra-high temperature (UHT) processing: Technological significance and updates
(Bentham Science Publishers, 2020) Prasad Rasane; Nitya Sharma; Sana Fatma; Sawinder Kaur; Alok Jha; Damanpreet Kaur; Jyoti Singh
Background: Milk forms an integral part of the human diet from the nutritional point of view. Besides nutrition, it has also unique functional properties which are harnessed by the industry for numerous uses. Being highly perishable specific techniques are required to minimize the losses during processing and adequate preservation of this precious commodity. In the U.S. and many other parts of the world, the traditional pasteurization of milk requires a minimum heat treatment of 72ºC for 15 seconds with subsequent refrigeration. However, the advent of Ultra High Temperature (UHT) treatment of milk has added a new dimension to the marketing of liquid milk in urban as well as re-mote areas without the requirement of cold chain management. The distinctive feature of UHT processed milk is that it is commercially-sterile-not pasteurized and so has long shelf life at room tem-perature. UHT milk, also known as long-life milk, is emerging as an attractive commercial alternative offering a hygienic product of unmatched quality, which can be bought anywhere, at any time and in any quantity. The present review will discuss numerous aspects of UHT processing of milk with reference to historical significance, fundamental principle, various systems used and prerequi-sites, type of exchangers used, fouling and other defects in system, chemical and microbiological effect of the treatment, its effect on nutritional components, organoleptic quality of milk and the ad-vantage and involved challenges of the process. Conclusion: Raw milk is easily contaminated with pathogens and microbes and hence its consumption of raw milk is associated with certain ill health effects. Therefore, heating milk before consumption is strongly suggested. Thus, UHT treatment of milk is done to ensure microbial safety and also to extend the shelf life of this highly perishable commodity. Heating milk at such a high temperature is often associated with the change of organoleptic properties like change in flavor or cooked flavor, rancidity due to microbes or acid flavor, etc. But UHT treatment does not substantially decrease the nutritional value or any other benefits of milk. © 2020 Bentham Science Publishers.