Browsing by Author "Sandeep Kumar"

Now showing 1 - 20 of 129

A non-covalently cross-linked self-healing hydrogel for drug delivery: characterization, mechanical strength, and anti-cancer potential
(Royal Society of Chemistry, 2024) Sheetal Jaiswal; Sandeep Kumar; Paramjeet Yadav; Krishtan Pal; Shere Afgan; Arvind Acharya; Ravi Prakash; Pralay Maiti; Rajesh Kumar
This research article introduced a novel one-pot method for synthesizing hydrogels, utilizing iron ions (Fe3+) and guar gum succinate (GGS) as cross-linkers. These hydrogels were characterized as cross-linked networks, with hydrogen bonds forming a sacrificial network and coordination bonds serving as the primary network. The reversible nature of these networks was attributed to the hydrogels’ exceptional toughness and remarkable self-healing properties. The hydrogel's chemical structure was confirmed through FTIR spectroscopy. XRD analysis highlighted the disruption of the crystalline nature of GGS upon cross-linking with Fe3+. By controlling the Fe3+ concentration, the hydrogels’ mechanical properties were tailored. Rheological measurements demonstrated mechanical and self-healing properties, while swelling studies revealed pH-dependent behavior. In vitro studies showed the hydrogels’ significant anti-proliferative effect against U-87MG (human glioblastoma) cancer cells, while remaining biocompatible with normal cell lines (HEK-293). These results indicated the potential application of these hydrogels in advancing cancer treatment strategies. © 2024 The Royal Society of Chemistry.
ABCD Rule for Breast Disease by Shukla Sandeep and Srivastava
(Springer, 2024) Hari Shankar Shukla; Sandeep Kumar; Anurag Srivastava
Breast diseases are on the rise throughout the world. It is essential to learn correct technique of “clinical breast examination” to ascertain the appropriate diagnostic workup in the most efficient manner. The ABCD rule is proposed by three highly skilled clinicians in the field of breast care trained in the famous Cardiff Breast Unit of Prof LE Hughes, Prof David JT Webster and Prof Robert E Mansel. The ABCD rule will allow early detection of breast cancer and other diseases with timely management of patients. It will help both the medical and nursing students as well as the field health workers in learning about diagnosis of breast diseases and in screening for breast cancer. © Association of Surgeons of India 2023.
Achyranthes aspera L. leaf extract induced anticancer effects on Dalton's Lymphoma via regulation of PKCα signaling pathway and mitochondrial apoptosis
(Elsevier Ireland Ltd, 2021) Rishi Kant Singh; Praveen Kumar Verma; Amit Kumar; Sandeep Kumar; Arbind Acharya
Ethnopharmacological relevance: Epidemiological studies promote the inclusion of natural-products in diet due to their inhibitory effects on various types of cancer. Among them, Achyranthes aspera L. (Family Amaranthaceae) is a medicinal plant in Ayurvedic pharmacopeia, found in India, Southeast Asia, America, and Sub-Saharan Africa. It is endowed with anti-inflammatory, anti-oxidant, and anti-cancer activities. However, its potential effect on Non-Hodgkin lymphomas (NHLs), has not yet been clarified. Aim of the study: In the present study, we aimed to investigate the effect of Achyranthes aspera L. leaf extracts on highly aggressive murine NHL called Dalton's Lymphoma (DL) in vitro and in vivo. Material and methods: GC-HRMS analysis was carried out for the identification of compounds present in A. aspera leaf extract. The cytotoxicity of various A. aspera leaf extracts was evaluated on DL cells by MTT assay. Chromatin condensation, nuclear fragmentation, and morphological changes were observed by microscopy technique. Flow cytometry was used to measure the changes in mitochondrial membrane potential (ΔΨm) and apoptosis. In addition, the expressions of apoptosis-related proteins were detected by western blotting. Meanwhile, the in vivo anti-tumor effect of leaf extract was tested in DL induced Balb/c mice. Result: GC-HRMS analysis of A. aspera methanolic leaf extract (AAML) revealed the presence of ten pharmacologically active compounds. The results showed that AAML suppressed cell proliferation, decreased mitochondrial membrane potential, changed the morphological structure, and induced apoptosis. Moreover, AAML could promote the release of cytochrome c by regulating Bcl-2 family proteins and then activated caspase-9/ -3 to triggered cell apoptosis. At the same time in DL cells treated with AAML, the protein kinase Cα (PKCα) pathway was inhibited in a concentration-dependent manner. Remarkably, in vivo, AAML mediated suppression of DL growth in Balb/c mice was accompanied by attenuation of the PKCα pathway and induction of apoptosis. Our result suggested that AAML promotes mitochondrial apoptotic cascade in DL cells by suppressing the PKCα signaling pathway. Conclusion: The study suggests that AAML could potently suppress DL progression by promoting apoptosis via mitochondrial-cascade and attenuation of the PKCα signaling pathway. © 2021
Advances in Legumes for Sustainable Intensification
(Elsevier, 2022) Ram Swaroop Meena; Sandeep Kumar
Advances in Legume-based Agroecoystem for Sustainable Intensification explores current research and future strategies for ensuring capacity growth and socioeconomic improvement through the utilization of legume crop cultivation and production in the achievement of sustainability development goals (SDGs). Sections cover the role of legumes in addressing issues of food security, improving nitrogen in the environment, environmental sustainability, economic-environmentally optimized systems, the importance and impact of nitrogen, organic production, and biomass potential, legume production, biology, breeding improvement, cropping systems, and the use of legumes for eco-friendly weed management. This book is an important resource for scientists, researchers and advanced students interested in championing the effective utilization of legumes for agronomic and ecological benefit. © 2022 Elsevier Inc. All rights reserved.
Agriculture ecosystem models for CO2 sequestration, improving soil physicochemical properties, and restoring degraded land
(Elsevier B.V., 2022) Ram Swaroop Meena; Ashutosh Yadav; Sandeep Kumar; Manoj Kumar Jhariya; Surendra Singh Jatav
Plans outlined in the conference of parties (COP) 26 included the restoration of degraded lands as one of the targets for achieving long-term food sustainability under climate change. The experiment aimed to assess carbon dioxide (CO2) sequestration and improvement in soil physicochemical properties of agriculture ecosystem models. The results of the experiment shows that the bulk density (ρb) and particle density (ρd) were significantly influenced at both depths 0–10 and 10–20 cm in developed ecosystems. The lowest (1.36 g cc−1) and highest (1.57 g cc−1) values of ρb were recorded at the depth of 0–10 cm in forest land (FL) and mono-cropping rice (Oryza sativa) cultivation land (MCRCL), respectively. The minimum values (kg ha−1) of N (161.27), P (14.87), and K (152.07) were recorded at the depth of 0–10 cm in mono-cropping wheat (Triticum aestivum) cultivated land (MCWCL), guava (Psidium guajava) + green gram (Vigna radiata) cultivation land (GGCL), and MCRCL, respectively. Moreover, the maximum N (207.60 kg ha−1) and P (19.27 kg ha−1) were recorded at the depth of 0–10 in FL system, and K (204.60 kg ha−1) in Karonda (Carissa carandas) cultivation land (KCL). The minimum soil N (158.93 kg ha−1) was recorded in pasture land (PL), while P (13.37 kg ha−1) and K (146.0 kg ha−1) was found in MCRCL system at the depth of 10–20 cm. While the highest soil organic carbon (SOC) stock was recorded in FL (18.40 Mg ha−1) and least in MCWCL system (6.57 Mg ha−1). The highest to the lowest value of CO2 sequestration (Mg ha−1) was found in the FL system (115.06) followed by KCL (41.11), GGCL (38.93), MCWCL (22.10), MCRCL (17.65), PL (9.40), and seasonal pond area (SPA 0.87). Likewise, the highest to lowest value of total C credit (US$) was found in FL (342.03) after that KCL (122.2), GGCL (115.71), MCWCL (65.7), MCRCL (52.45), PL (27.94), and SPA (2.6), respectively. With the creation of agricultural ecosystem models on degraded land, this work gives a roadmap for repairing degraded land, enhance the terrestrial CO2 sequestration, C-credit, and boosting ecological services, which may contribute to attaining long-term food sustainability. © 2022
Agriculture models for restoring degraded land to enhance CO2 biosequestration and carbon credits in the Vindhyan region of India
(Elsevier B.V., 2024) Ram Swaroop Meena; Gourisankar Pradhan; Kanchan Singh; Sandeep Kumar; Ambuj Kumar Singh; K.S. Shashidhar; Krishan Kant Mina; Ch. Srinivasa Rao
The study's objective was to evaluate the status of converted degraded land into productive agricultural models by improving the physicochemical properties of the soil, soil organic matter (SOM), soil organic carbon (SOC) fractions (active and passive), and microbial biomass carbon (MBC), while also generating carbon (C) credit for additional farmers' income. Six models were analyzed, namely: (1) Arjun forest-based agroecosystems (AFBAE); (2) Lemon grass-based agroecosystems (LGBAE); (3) Legume-cereal-moong-based agroecosystems (LCMBAE); (4) Bael-black mustard-based agroecosystems (BMBAE); (5) Guava-wheat-based agroecosystems (GWBAE), and (6) Custard apple -lentil -based agroecosystems (CALBAE). These models were replicated three times in a randomized block design (RBD). Soil samples were collected from the study area at two depths (0–0.30 and 0.30–0.60 m). At a 0–0.30 m depth, the highest bulk density (ρb) of 1.50 Mg m−3 was observed in LCMBAE, while the lowest ρb of 1.43 Mg m−3 was recorded in BMBAE. The soil organic carbon (SOC) and SOC stock values exhibited a range of 4.2–7.7 g kg−1 and 19.0–33.4 Mg ha−1, respectively. In the AFBAE, the highest levels of 163.1 % MBC were found over LCMBAE. At a 0–0.30 m depth, the recalcitrant index (RI) and lability index (LI) ranged from 0.35–0.46 to 1.97–2.11, respectively. Additionally, the AFBAE exhibited the highest total biomass accumulation (39.23 Mg ha−1), carbon dioxide (CO2) biosequestration (287.9 Mg ha−1), and the total social cost of CO2 at US$ 277 ha−1. Furthermore, in the AFBAE, there was a 198.1 % increase in total C credit (US$ 161 ha−1) compared to LCMBAE (US$ 54 ha−1). However, at 0.30–0.60 m depths, GWBAE and CALBAE were statistically equivalent (p ≤ 0.05) in total C stocks. Principal component analysis (PCA) reveals that component-1 accounts for 77.4 % of the variability, while component-2 contributes 18.6 %. This article aimed to convert the degraded land into a sustainable agricultural module by increasing SOC and CO2 biosequestration and producing more C-credit, or climate currency, on underutilized land. © 2024 Elsevier B.V.
Agroecology Towards Environmental Sustainability
(Springer Singapore, 2021) Shailesh Kumar Yadav; Arnab Banerjee; Manoj Kumar Jhariya; Abhishek Raj; Nahid Khan; Ram Swaroop Meena; Sandeep Kumar
Agroecology refers to the process based on ecological principles to be applied in the agroecosystem for effective soil management and gain sustainable yield. The scientific application leads to a diversified agroecosystem which addresses the issue of environmental sustainability. It also focuses on various ecosystemservices in the form of maintaining soil fertility, proper biogeochemical cycling, and proper nutrient exchange between crop and soil ecosystem. The process ncludes an integrated approach with diversified crops and animal husbandry practices all at a time. Thus, it would be successful to address the issue of food security, crisis, and help to build up climate-resilient agroecosystem. Agroecosystem is also helpful in terms of maintaining a daily livelihood, production of fuel, fodder, food for rural stakeholders, and socioeconomic well-being of people across the globe. Thus, agroecological addresses the sustainable agriculture practice on a large scale to promote eco-friendly, self-sustaining agriculture practices. The aim of this article is to reflect an all-round aspect of agroecologyn along with its roadmap towards environmental sustainability. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021.
Agroforestry and ecosystem services
(Elsevier, 2023) Abhishek Raj; Manoj Kumar Jhariya; Arnab Banerjee; Ram Swaroop Meena; Sandeep Kumar; Annpurna Devi; Poonam
Agroforestry system (AFs) comprises tree–crop and livestock management that has been considered as an integrated system of sustainable landscape. It is more diverse and provides uncountable ecosystem services (ES) to sustain life on the earth. The greater adaptability and multifunctional role of AFs are discussed by policymakers, stakeholders, and scientists worldwide. Traditional AFs and their development in due course of time maximize ES. AFs provide tangible and intangible services which maintain ecosystem health and productivity. Adopting climate-resilient AFs ensures soil-food and income security, fulfilling the SDGs (sustainable development goals). Timber and NTFPs (non-timber forest products) production, soil health and quality enhancement, water regulation and quality, carbon (C) footprint, climate change mitigation, and food and income security are the key ES AFs provide. AFs also contribute toward ecological stability along with better environmental health and sustainability. A better technological advancement with effective policy is needed to strengthen AFs in major ecological regions. Moreover, current and future research trends must be oriented to enhance ES through greater crop diversification. Thus, AFs must be transformed into sustainable landscapes at local and global soil–food–climate security. © 2024 Elsevier Inc. All rights reserved.
Agroforestry for Carbon and Ecosystem Management
(Elsevier, 2023) Manoj Kumar Jhariya; Ram Swaroop Meena; Arnab Banerjee; Sandeep Kumar; Abhishek Raj
Agroforestry for Carbon and Ecosystem Management is a comprehensive overview of current research, issues, challenges, and case studies in the area of agroforestry. The book focuses specifically on carbon source-sink relationship and management through agroforestry practices with a goal of improving overall environmental sustainability. Through expert insights and case studies, the book promotes carbon management, greenhouse gas emission reduction, forest, and ecosystem services management, along with relevant sustainable approaches for natural resources conservation. Users will find insights into novel approaches for natural resource management, with specific attention given to technologies related to carbon capture and management. In addition, the book addresses the knowledge gap in relation to agroforestry, sustainability, and agroecosystem management and explores the application of remote sensing and geospatial technologies for agroforestry management. © 2024 Elsevier Inc. All rights reserved.
Agroforestry for carbon and ecosystem management: an overview
(Elsevier, 2023) Manoj Kumar Jhariya; Ram Swaroop Meena; Arnab Banerjee; Sandeep Kumar; Abhishek Raj
Agroforestry is a land use practice that provides environmental protection and ecological restoration of degraded habitats. Globally it plays a vital role in carbon (C) and ecosystem management and helps to achieve food, nutritional, economic, and environmental security. Combating mega events in this human-altered world has necessitated the practice of agroforestry to cope with changing climate. Further, agroforestry tends to rehabilitate various forms of degraded lands and ecosystems. The agricultural system is the production of crops and livestock management. Agroforestry also offers extra advantages by increasing biological productivity. Consequently, agroforestry is more widely accepted by the scientific community as a producer of many ecosystem services. The potential of agroforestry systems for efficient C management, ecosystem services, and ecological restoration of damaged ecosystems was demonstrated through research evidence. To preserve the overall integrity of the ecosystem, agroforestry has also demonstrated tremendous promise in managing plant and soil C pools through appropriate biomass addition in the soil ecosystems to maintain the environmental sustainability. © 2024 Elsevier Inc. All rights reserved.
An improved method of bisulfite treatment and purification to study precise DNA methylation from as little as 10 pg DNA
(2012) Sandeep K. Rajput; Sandeep Kumar; Vivek P. Dave; Ankita Rajput; Haushila P. Pandey; Tirtha K. Datta
Methylation of vertebrate DNA is one of the most important epigenetic alterations which have become a center of scientific attraction, especially because of its important role in the regulation of transcription, genomic imprinting, developmental process, and pathogenesis of various diseases. Currently, there are wide ranges of methods available to produce quantitative and qualitative information on genomic DNA methylation. The vast majority of these methods rely on the optimization of the efficient bisulfite treatment. However, all the available methods for bisulfite treatment suffer from major disadvantages, such as large amount of starting material, poor conversion efficiency as well as low recovery and integrity of DNA after bisulfite treatment. Here, we developed a simple, rapid, and convenient column-based bisulfite treatment method by improving the several critical steps, which leads to consistent C-to-U conversion rate 99-100%, >75% recovery of DNA after bisulfate treatment. In addition, it is commercially viable and requires very less amount (∼10 pg) of DNA. © Springer Science+Business Media, LLC 2012.
Assessment of land use systems for CO2 sequestration, carbon credit potential, and income security in Vindhyan region, India
(John Wiley and Sons Ltd, 2022) Oindrila Roy; Ram Swaroop Meena; Sandeep Kumar; Manoj Kumar Jhariya; Gourisankar Pradhan
Land-use systems (LUSs) in the agriculture urgently need to be revised to reduce greenhouse gases emissions (GHGs), and promote long-term sustainability. This study aimed to estimate the highest amount of CO2 sequestration, carbon (C) stocks, and credit potential to mitigate climate change, and importantly identify a more sustainable LUS for income security, which can be easily adopted by farmers. The experiment was executed with the current year six LUSs: (1) legumes cereal wheat (Triticum aestivum) -based LUS (LCBLUS); (2) vegetable (cabbage -Brassica oleracea varcapitata) -based LUS (VBLUS) (farmer field); (3) guava (Psidium guajava) + linseed (Linum usitatissimum) -based LUS (GLBLUS); (4) custard apple (Annona reticulata) + barley (Hordeum vulgare) -based LUS (CABBLUS); (5) bael (Aegle marmelos) + mustard (Brassica juncea) -based LUS (BMBLUS), and (6) teak (Tectona grandis) -based forest LUS (TBFLUS) with four-times replicated in a randomized block design (RBD). The soil samples were collected at 0–15 and 15–30 cm depths from the study area at the start, and end of the experiment. Results revealed that the mean value of a bulk density (ρb) and particle density (ρd) was ranged from 1.38–1.54 Mg m−3 and 2.40–2.65 Mg m−3, respectively. The mean value of pH and EC was ranged from 4.49–5.84 and 0.07–0.21 dsm-1, respectively. Soil organic carbon (SOC) was ranged from 0.48%–0.76% and the total NPK stock range was from 51.69–58.97 Mgha-1. The biomass accumulation, C stock, sequestration potential, and credit for six LUSs were ranged from 9.39–75.82, 4.69–37.91, 17.23–139.93 Mgha-1, and 689–5565 US$ha-1, respectively. The highest biomass accumulation, C stock, sequestration, and credit potential were observed under TBFLUS and the lowest under LCBLUS. The highest estimated market price was 5583.14 US$ha-1 from BMBLUS, followed by CABBLUS (5284.42 US$ha-1), GLBLUS (5121.70 US$ha-1), and VBLUS (4198.40 US$ha-1). While the minimum price market price was 969.83 US$ha-1 recorded from LCBLUS. According to the results of this experiment, the TBFLUS had the highest soil enrichment, maximum C storage, and CO2 sequestration capacity among all the LUSs. © 2021 John Wiley & Sons, Ltd.
Bayesian estimation of the number of species from Poisson-Lindley stochastic abundance model using non-informative priors
(Springer Science and Business Media Deutschland GmbH, 2024) Anurag Pathak; Manoj Kumar; Sanjay Kumar Singh; Umesh Singh; Sandeep Kumar
In this article, we propose a Poisson-Lindley distribution as a stochastic abundance model in which the sample is according to the independent Poisson process. Jeffery’s and Bernardo’s reference priors have been obtaining and proposed the Bayes estimators of the number of species for this model. The proposed Bayes estimators have been compared with the corresponding profile and conditional maximum likelihood estimators for their square root of the risks under squared error loss function (SELF). Jeffery’s and Bernardo’s reference priors have been considered and compared with the Bayesian approach based on biological data. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Bayesian inference for Maxwell Boltzmann distribution on step-stress partially accelerated life test under progressive type-II censoring with binomial removals
(Springer, 2022) Anurag Pathak; Manoj Kumar; Sanjay Kumar Singh; Umesh Singh; Manoj Kumar Tiwari; Sandeep Kumar
This article deals with the estimation problem in step-stress partially accelerated life test of Maxwell Boltzmann distribution in presence of progressive type-II censoring with binomial removals. The maximum likelihood and Bayes estimators of the parameter are obtained under symmetric and asymmetric loss functions. Furthermore, the performances of the obtained estimators are compared in terms of risks. The proposed methodology is illustrated through the time to failure (in days) of Aluminium reduction cells and survival times (in weeks) for male rats that were exposed to a high level of radiation. © 2022, The Author(s) under exclusive licence to The Society for Reliability Engineering, Quality and Operations Management (SREQOM), India and The Division of Operation and Maintenance, Lulea University of Technology, Sweden.
Benign breast disorders in nonwestern populations: Part II-Benign breast disorders in India
(Springer-Verlag, 1989) Hari S. Shukla; Sandeep Kumar
Experience with benign breast disorders has been analyzed in 3 nonwestern populations: Hong Kong, India, and Northern Nigeria. Similarities to and differences from Western experience are found, but of great interest are notable differences between these populations which, as yet, lack explanation. All show "fibroadenosis" and fibroadenoma as common conditions, but the frequency with which phyllodes tumor is diagnosed varies between different centers in India as well as between different racial groups. Tuberculosis is another interesting example-wide differences in the frequency of breast infection are found although tuberculosis itself is common in all 3 countries. The value of prospective studies was shown when mastalgia was studied in this way in India. Often considered a "Western" affliction, these authors have been able to study 112 cases of mastalgia and found it to be twice as common as cancer as a presentation. These differing experiences between populations have been little explored and must hold promise for unravelling some of the enigmas of benign breast disorders in all countries. © 1989 Société Internationale de Chirurgie.
Benzimidazole-derived carbohydrazones as dual monoamine oxidases and acetylcholinesterase inhibitors: design, synthesis, and evaluation
(Taylor and Francis Ltd., 2024) Sandeep Kumar; Shivani Jaiswal; Sukesh Kumar Gupta; Senthil Raja Ayyannan
A series of novel benzimidazole-derived carbohydrazones was designed, synthesized and evaluated for their dual inhibition potential against monoamine oxidases (MAOs) and acetylcholinesterase (AChE) using multitarget-directed ligand approach (MTDL). The investigated compounds have exhibited moderate to excellent in vitro MAOs/AChE inhibitory activity at micromolar to nanomolar concentrations. Compound 12, 2-(1H-Benzo[d]imidazol-1-yl)-N'-[1-(4-hydroxyphenyl) ethylidene]acetohydrazide has emerged as a lead dual MAO-AChE inhibitor by exhibiting superior multi-target activity profile against MAO-A (IC50 = 0.067 ± 0.018 µM), MAO-B (IC50 = 0.029 ± 0.005 µM) and AChE (IC50 = 1.37 ± 0.026 µM). SAR studies suggest that the site A (hydrophobic ring) and site C (semicarbazone linker) modifications attempted on the semicarbazone-based MTDL resulted in a significant enhancement in the MAO-A/B inhibitory potential and a drastic decrease in the AChE inhibitory activity. Further, molecular docking and dynamics simulation experiments disclosed the possible molecular interactions of inhibitors inside the active site of respective enzymes. Also, computational prediction of drug-likeness and ADME parameters of test compounds revealed their drug-like characteristics. Communicated by Ramaswamy H. Sarma. © 2023 Informa UK Limited, trading as Taylor & Francis Group.
Biochemical markers of ketosis in dairy cows at post-parturient period
(Taylor and Francis Ltd., 2021) Mohammed Nazeer; Sandeep Kumar; Manu Jaiswal
Forty cows suffered from ketosis at post-parturient period were used in the current study together with 10 healthy cows who served as a control. Blood samples were collected from diseased and healthy animals and the harvested serum were used for determination of biochemical, the profiles of lipids and liver enzymes. The obtained results declared that serum insulin had highly significant decrease (P < 0.01) in group C (5.02 ± 0.46 µIU/ml), also there was a highly significant decrease in serum cortisol (P < 0.01) of both A and B groups (3.74 ± 0.15 and 3.98 ± 0.14 ng/ml), as compared to healthy cows (5.39 ± 0.27 ng/ml). The values of serum cholesterol, serum triglyceride and serum total lipids were significantly increased (P < 0.01) in groups A, B and C 146.54 ± 7.71, 153.22 ± 6.04 and 150.50 ± 6.02 mg/dl; 74.85 ± 0.95, 76.43 ± 1.85 and 80.05 ± 4.68 mg/dl and 271.31 ± 4.34, 281.76 ± 3.50 and 266.64 ± 4.26 mg/dl, respectively; also, the values of serum aspartate aminotransferase and alanine aminotransferase were highly significantly increased (P < 0.01) in ketotic cows of groups A, B and C (94.13 ± 1.05, 94.42 ± 1.29 and 88.30 ± 88.30 ± 3.44 IU/L, respectively, and 57.07 ± 3.97, 54.29 ± 4.40 and 64.20 ± 4.70 IU/L, respectively). There was a highly significant decrease (P < 0.01) in serum calcium of groups A, B and C (7.20 ± 0.23, 7.15 ± 0.14 and 8.95 ± 0.33 mg/dl, respectively). © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Chitosan-based self-healing hydrogel mediated by poly(acrylic-methacrylic acid) exhibiting high biocompatibility and anti-tumor activity
(John Wiley and Sons Inc, 2024) Krishtan Pal; Sandeep Kumar; Paramjeet Yadav; Sheetal Jaiswal; Rajesh Kumar; Arbind Acharya
Multi-responsive, self-healing hydrogels were developed utilizing positively charged polysaccharides, chitosan, and water-soluble chitosan. This fabrication employed free-radical synthesized polyacrylic acid and polymethacrylic acid along with the cross-linker FeCl3, resulting in the generation of polyelectrolyte metal complexes, which enhances the properties of the chitosan-based hydrogels, particularly their mechanical strength, self-healing ability, thermal stability, swelling behavior, porous structure, cell viability, and even anticancer activity. Characterization and stability assessment of the hydrogels were performed using FT-IR, nuclear magnetic resonance, gel permeation chromatography, scanning electron microscopy, rheometer, x-ray diffraction, thermogravimetric analysis, DSC, and UV spectroscopy. FT-IR measurements indicated that the facile complexation of the cross-linker's metal ions (Fe3+) with the carboxylate (COO−), amino (NH), and hydroxyl (OH) groups of the polymers and chitosan chains facilitated rapid gelation. Furthermore, the sustained release of the drug levofloxacin (up to 80%) was observed to increase with increasing pH due to the hydrogels' anionic nature. Biocompatibility and cytotoxicity tests were conducted using the MTT assay on splenocytes and Dalton Lymphoma cancer cell lines. These tests demonstrated the promising potential of these hydrogels for drug delivery applications. © 2024 Wiley Periodicals LLC.
Cleomiscosin D, a coumarino-lignan from seeds of Cleome viscosa
(1988) Sandeep Kumar; Anil B. Ray; Chohachi Konno; Yoshiteru Oshima; Hiroshi Hikino
Cleomiscosin D, a minor coumarino-lignan of the seeds of Cleome viscosa, has been proved to be regioisomer of cleomiscosin C. A method of degradation of coumarino-lignans for the identification of the coumarin moiety has been developed. © 1988.
Cobalt (III) complex exerts anti-cancer effects on T cell lymphoma through induction of cell cycle arrest and promotion of apoptosis
(Springer Science and Business Media Deutschland GmbH, 2022) Praveen Kumar Verma; Rishi Kant Singh; Sandeep Kumar; Alok Shukla; Sanjay Kumar; Mannu Kumar Gond; Manoj Kumar Bharty; Arbind Acharya
Purpose: Cobalt-based compounds are emerging as a non-platinum-based anti-cancer effective therapeutic agent. However, there is a limited study regarding the therapeutic efficacy of Cobalt-based drugs against Non-Hodgkin’s Lymphoma (NHLs) such as T cell lymphoma. Therefore, in the present study we investigated the anti-tumor role of cobalt(III) complex [Co(ptsm)NH3(o-phen)]·CH3OH on Dalton’s Lymphoma (DL) cells. Materials and methods: Cytotoxicity of the cobalt complex was estimated by MTT assay. Analysis of mitochondrial membrane potential, cell cycle and Reactive oxygen species (ROS) generation, and Annexin V/PI staining was done by Flow cytometry, while AO/EtBr staining by fluorescence microscopy in cobalt complex treated DL cell. Expression of cell cycle and apoptosis regulatory protein was analyzed by Western blotting. In addition, in vivo study of the cobalt complex was evaluated in well-established DL bearing mice by monitoring physiological parameters and mean survival time. Results: Our study showed that cobalt complex triggered apoptosis and induced cell cycle arrest in DL cells. Furthermore, this also decreased mitochondrial membrane potential and increased intracellular ROS generation in cancer cells. In addition, changed expression of cell cycle and apoptosis regulatory protein was found with enhanced activity of caspase-3 and 9 in the treated cells. Additionally, administration of cobalt complex showed a significant increase in the survivability of tumor-bearing host, which was accomplished by decreasing physiological parameters. Conclusion: Taken together, these data revealed anti-tumor potential of cobalt complex against DL cells through cell cycle arrest and mitochondrial-dependent apoptosis. Henceforth, cobalt-based drugs could be a new generation therapeutic drug to treat hematological malignancies. Graphical abstract: [Figure not available: see fulltext.]. © 2022, Springer Nature Switzerland AG.