Browsing by Author "Sandeep Kumar Rajput"

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Detection and diagnostic applicability of human urinary kininogen in kala-azar patients
(2012) Vinod Kumar; Manish Mishra; Sandeep Kumar Rajput; Surabhi Bajpai; Rakesh K. Singh
The present study was aimed to detect urinary proteins excreted in kala-azar patients. Urinary proteins were isolated by ammonium sulfate precipitation and purified by Amicon ultra using 3 kDa cutoff membrane device. The proteins were resolved on 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis and blots were developed with the patients sera. The excretion of urinary proteins was differential in kala-azar patients. A total eight proteins of molecular weights 25, 28, 46, 54, 57, 60, 72, and 95 kDa were detected in the urine of visceral leishmaniasis (VL) patients. On blot, the 57 kDa protein was found to be host origin and characterized as human kininogen. All other proteins were leishmanial origin. Out of 50 urine samples analyzed, the kininogen was detected in 45 urine samples. Following treatment, this protein was not detectable in the urine samples of any patient. The appearance of kininogen in urine of VL patients offers a novel possibility for the development of diagnostic tool and a test of cure. ©Springer-Verlag 2012.
P53 gene: Mutation and immunohistochemical analysis in patients with invasive ductal carcinoma of breast
(2013) Shinjini Singh; Sandeep Kumar Rajput; Mritunjai Singh; Pravas Kumar Misra; Gyanendra Mohan; Mohan Kumar; Rakesh Kumar Singh; Indrajeet Singh Gambhir
The p53 tumor suppressor gene is the most commonly mutated gene in cancer. In breast cancer, the presence of p53 gene alterations has been associated with worse prognosis. This study was attempted to associate p53 gene mutations with its protein expression in North Eastern Indian population. We used single-stranded conformation polymorphism to screen samples for mutations in five conserved regions, exons 4, 5, 6, 7 and 8, of the p53 gene. Mutations were confirmed by direct DNA sequencing. Samples were also analyzed for expression of p53 immunohistochemically. We found two critical mutations in the exon 4. A well known missense mutation at codon 72 (pro to arg) with a frequency of 47% was found which was significantly correlated with the immunohistochemical analysis of p53 protein in such patients. A novel nonsense mutation at codon 107 which leads to stop codon was also found. Although the occurrence of this mutation was very less, we did not find expression of p53 protein immunohistochemicaly. We support that mutation in p53 gene can be exploited as a prognostic marker for the early diagnosis of breast cancer, although more clinical and epidemiological data is required to establish this claim. © 2013 Science Publication.