Browsing by Author "Sangita Lahiry"

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Energy Dispersive X-Ray Fluorescence (EDXRF) study of the blood samples of Indian Kala-Azar patients
(Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2016) Sangita Lahiry; Rajiv Kumar; Shyam Sundar; Anindita Chakraborty; Mathummal Sudharshan; Madhumita Manna
Leishmaniasis is a disease spectrum caused by the protozoan parasites of Genus Leishmania. In this study, trace elements concentrations (K, Fe, Cu, Zn, Cl, S, Ca, Mn, Rb & Br) of twenty two Kala-azar (KA) patients were measured by Energy Dispersive X-Ray Fluorescence (EDXRF) Spectrophotometer along with six healthy control (HC) (n=28). Significant decrease in the levels of Fe and Zn and increase in level of Cu were found in untreated groups (UT) of KA patients compared to that of HC group (P < 0.001, P < 0.001 and P < 0.01, respectively). Treated group of patients (TR) who received Amphotericin B, showed significant increase in levels of Fe and Zn. Comparison between TR and UT groups for Fe and Zn showed statistically improved status but changes in Cu level was not statistically significant. Meanwhile, no significant difference in level of other trace elements was observed in all experimental groups. Among the ten trace element profiles, Cu, Fe and Zn imbalances were very clear in active KA patients. It corroborated earlier findings including ours. These changes came back to almost normal level after Amphotericin B therapy. Thus drug treatment stabilizes trace elements levels which may act as useful marker for the disease.
Particle induced X-ray emission study of blood samples of Indian Kala-azar patients
(Springer India, 2017) Sangita Lahiry; Supriya Khanra; Rajiv Kumar; Anindita Chakraborty; Shyam Sundar; Mathummal Sudarshan; Madhumita Manna
Visceral leishmaniasis (VL) or Kala-azar (KA) is a neglected tropical disease caused by protozoan parasite, Leishmania sp. and is fatal, if left untreated. In this study, we measured trace elements (K, Fe, Cu, Zn, Br, Cl, S, Ca, Mn, Cr, Ni, As, Se, Rb and Sr) in the blood of Indian VL patients (32) by particle-induced X-ray emission (PIXE) study. Blood was collected from 36 subjects including healthy controls from Rambagh Kala-azar Hospital, Muzaffarpur, Bihar, India. PIXE experiment was carried out at the Institute of Physics, Bhubaneswar, India and data were analyzed by GUPIXWIN software. We observed first time the association of bromine with the disease. The results showed 48.47 % decrease in Br, 35.16 % decrease in Zn and 29.05 % decrease in Fe in untreated state of the KA patients. In the same group, Cu has been increased by 16.73 %. Cu/Zn ratio has been altered in diseased state. The association of bromine with the disease is reported for the first time and altered levels of trace elements (Br, Cu, Fe and Zn) may come back to normal after completion of the treatment regimen with Amphotericin B. © 2016, Indian Society for Parasitology.