Browsing by Author "Sanjay Kumar Ojha"

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Evaluation of biomethane potential of codigested sheep manure and kitchen refuse
(Springer Science and Business Media Deutschland GmbH, 2023) Puneet Kumar Singh; Haragobinda Srichandan; Sanjay Kumar Ojha; Ritesh Pattnaik; Sunil Kumar Verma; Sreyasi Pal; Jagriti Singh; Snehasish Mishra
A study on the biomethanation potential of codigested sheep manure (SM) and kitchen refuse (KR) was carried out. Both the substrates varied in their C:N ratio, cellulose, hemicellulose and lignin contents, although they had very similar moisture and volatile solid (VS) contents. Anaerobic codigestion to balance the C:N ratio thereby making the process more efficient to help enhance biomethanation is a universally accepted strategy. Various combinations (80:20, 60:40, 40:60, 20:80) were used for biogas production alongside pure SM (100:0) and KR (0:100) to arrive at an efficient ratio using the digested slurry from an active biodigestor as the microbial inoculum. A 40-day experiment was set at 40 °C and pH 7.0 initial conditions, and the effect of the codigestion on the pH, volatile fatty acids (VFA) and the C:N ratio was evaluated. It was observed that the biogas and methane generation were maximal at SM:KR::40:60 combination with 7.0–7.5 pH, 23.3 C:N ratio, and a balanced VFA production and utilisation. The maximum cumulative biogas and methane generated were 2575 mL and 1660 mL, and the calculated biogas and methane generation based on the VS content were 209.35 and 135.13 mL/g VS. Based on the results obtained from the study, it is suggested that sheep manure and kitchen refuse codigestion could help enhance biogas production at 40:60 combination. Although reports on codigestion of other such biogasifiable substrates exist, similar studies could be tried for numerous other nonconventional organic biogasifiable substrates to validate their biomethanation potentials and enhancements. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Primary and acquired drug resistance patterns of Mycobacterium tuberculosis isolates in India: A multicenter study
(2013) Jitendra Prasad Mathuria; Jai Kumar Samaria; Govind Narayan Srivastava; Bharat Lal Mathuria; Sanjay Kumar Ojha; Shampa Anupurba
Tuberculosis is the most prevalent infection worldwide. The emergence of drug-resistant Mycobacterium tuberculosis (M. tuberculosis) isolates emphasizes that it is necessary to monitor drug resistance of the organism against anti-tubercular drugs. We analyzed 327 M. tuberculosis isolates from patients who were cared for at three different health care centers, hereinafter known as study areas (SAs), in North India. Of the 327 total M. tuberculosis isolates, 255 were from a tertiary health care center (Varanasi, Uttar Pradesh [SA-1]), 48 were from a District tuberculosis center (Sawai Madhopur, Rajasthan [SA-2]), and 24 were from a different District tuberculosis center (Buxar, Bihar [SA-3]). Drug susceptibility testing against first-line antibiotics (viz. isoniazid, rifampicin, streptomycin, and ethambutol) was conducted for all the isolates using 1% proportional method. We found that the rates of acquired resistance were consistently higher than the rates of initial drug resistance. In new, untreated cases, a higher degree of MDR-TB was observed at SA-1 (13.3%) and SA-3 (25.0%), whereas it was observed in only 7.1% of the isolates at SA-2. In previously treated patients, MDR cases were found in 35.7% of the isolates from SA-1, 66.6% of the isolates from SA-2, and 43.8% of the isolates from SA-3. Resistance to a single drug was found at a much lower rate, ranging from 0.0 to 6.3% in new cases as well as previously treated cases. In conclusion, the primary resistance of M. tuberculosis is low, but acquired drug resistance is slightly higher in North India. © 2013 King Saud Bin Abdulaziz University for Health Sciences.