Browsing by Author "Sanjay Kumar Singh Patel"

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Correction to: Production, optimization, scale up and characterization of polyhydoxyalkanoates copolymers utilizing dairy processing waste (Scientific Reports, (2024), 14, 1, (1620), 10.1038/s41598-024-52098-0)
(Nature Research, 2024) Tejaswini Dhanaji Patil; Saptaneel Ghosh; Aparna Agarwal; Sanjay Kumar Singh Patel; Abhishek Dutt Tripathi; Dipendra Kumar Mahato; Pradeep Kumar; Petr Slama; Ales Pavlik; Shafiul Haque
Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-024-52098-0, published online 18 January 2024 The Author Contributions section in this Article was incomplete. “T.D.P., A.D.T., P.S., A.P., S.H. and S.K.S.P. conceptualized the experiment. T.D.P., A.D.T. and A.A did the methodology and validation. T.D.P., A.D.T. conducted the formal analysis and investigation. T.D.P., A.D.T., and S.K.S.P. wrote and prepared the original draft. A.D.T., P.S., A.P., S.H., and S.K.S.P. conducted the writing, review and editing. A.A. helped in visualization. A.D.T. and A.A. supervised the experiment. S.H. and D.K.M. edited the manuscript. T.D.P., A.D.T. and A.A. performed analytical work. All authors have read and agreed to the published version of the manuscript.” now reads, “T.D.P., A.D.T., P.S., A.P., S.H. and S.K.S.P. conceptualized the experiment. T.D.P., A.D.T. and A.A. did the methodology and validation. T.D.P., A.D.T. conducted the formal analysis and investigation. T.D.P., A.D.T., and S.K.S.P. wrote and prepared the original draft. A.D.T., P.S., A.P., S.H., and S.K.S.P. conducted the writing, review, and editing. A.A. helped in visualization. A.D.T. and A.A. supervised the experiment. S.H. and D.K.M. edited the manuscript. T.D.P., A.D.T., P.K., and A.A. performed analytical work. All authors have read and agreed to the published version of the manuscript.” The original Article has been corrected. © The Author(s) 2024.
Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria
(MDPI, 2022) Lokender Kumar; Sanjay Kumar Singh Patel; Kusum Kharga; Rajnish Kumar; Pradeep Kumar; Jessica Pandohee; Sourabh Kulshresha; Kusum Harjai; Sanjay Chhibber
Microbial biodiversity includes biotic and abiotic components that support all life forms by adapting to environmental conditions. Climate change, pollution, human activity, and natural calamities affect microbial biodiversity. Microbes have diverse growth conditions, physiology, and metabolism. Bacteria use signaling systems such as quorum sensing (QS) to regulate cellular interactions via small chemical signaling molecules which also help with adaptation under undesirable survival conditions. Proteobacteria use acyl-homoserine lactone (AHL) molecules as autoinducers to sense population density and modulate gene expression. The LuxI-type enzymes synthesize AHL molecules, while the LuxR-type proteins (AHL transcriptional regulators) bind to AHLs to regulate QS-dependent gene expression. Diverse AHLs have been identified, and the diversity extends to AHL synthases and AHL receptors. This review comprehensively explains the molecular diversity of AHL signaling components of Pseudomonas aeruginosa, Chromobacterium violaceum, Agrobacterium tumefaciens, and Escherichia coli. The regulatory mechanism of AHL signaling is also highlighted in this review, which adds to the current understanding of AHL signaling in Gram-negative bacteria. We summarize molecular diversity among well-studied QS systems and recent advances in the role of QS proteins in bacterial cellular signaling pathways. This review describes AHL-dependent QS details in bacteria that can be employed to understand their features, improve environmental adaptation, and develop broad biomolecule-based biotechnological applications. © 2022 by the authors.
Nutritional and Functional New Perspectives and Potential Health Benefits of Quinoa and Chia Seeds
(Multidisciplinary Digital Publishing Institute (MDPI), 2023) Aparna Agarwal; Rizwana; Abhishek Dutt Tripathi; Tarika Kumar; Kanti Prakash Sharma; Sanjay Kumar Singh Patel
Quinoa (Chenopodium quinoa Willd) and chia (Salvia hispanica) are essential traditional crops with excellent nutritional properties. Quinoa is known for its high and good quality protein content and nine essential amino acids vital for an individual’s development and growth, whereas chia seeds contain high dietary fiber content, calories, lipids, minerals (calcium, magnesium, iron, phosphorus, and zinc), and vitamins (A and B complex). Chia seeds are also known for their presence of a high amount of omega-3 fatty acids. Both quinoa and chia seeds are gluten-free and provide medicinal properties due to bioactive compounds, which help combat various chronic diseases such as diabetes, obesity, cardiovascular diseases, and metabolic diseases such as cancer. Quinoa seeds possess phenolic compounds, particularly kaempferol, which can help prevent cancer. Many food products can be developed by fortifying quinoa and chia seeds in different concentrations to enhance their nutritional profile, such as extruded snacks, meat products, etc. Furthermore, it highlights the value-added products that can be developed by including quinoa and chia seeds, alone and in combination. This review focused on the recent development in quinoa and chia seeds nutritional, bioactive properties, and processing for potential human health and therapeutic applications. © 2023 by the authors.
Production, optimization, scale up and characterization of polyhydoxyalkanoates copolymers utilizing dairy processing waste
(Nature Research, 2024) Tejaswini Dhanaji Patil; Saptaneel Ghosh; Aparna Agarwal; Sanjay Kumar Singh Patel; Abhishek Dutt Tripathi; Dipendra Kumar Mahato; Pradeep Kumar; Petr Slama; Ales Pavlik; Shafiul Haque
The microbial biotransformation using low-cost feedstock to produce biopolymers (degradable), an alternative to petrochemical-based synthesis plastics (non-degradable), can be a beneficial approach towards sustainable development. In this study, the dairy industry processes waste (whey) is used in polyhydroxyalkanoate (PHA) copolymer production. Initial screening suggested that Ralstonia eutropha produced higher PHA as compared to Bacillus megaterium. A central composite rotatable design-based optimization using two process variables (amino acid and tween-80) concentration remarkably influenced PHA co-polymer production under physiological conditions of pH (7), temperature (37 °C), and agitation rate of 150 rpm. High polyhydroxybutyrate (PHB) mass fraction yield of 69.3% was observed as compared to predicted yield of 62.8% from deproteinized whey as feed. The combination of tryptophan (50 mg L−1) and tween-80 (3 mL−1) enhanced R. eutropha mass gain to 6.80 g L−1 with PHB contents of 4.71 g L−1. Further, characterization of PHA and its copolymers was done by ESI–MS, FTIR, and TEM. On upscaling up to 3.0 L, the PHA contents and yields were noted as quite similar by R. eutropha. This study demonstrates that dairy waste processing waste can be potentially utilized as inexpensive feed for producing high content of biopolymers to develop a sustainable system of waste management. © 2024, The Author(s).
Valorization of Wastewater Resources Into Biofuel and Value-Added Products Using Microalgal System
(Frontiers Media S.A., 2021) Kanika Arora; Parneet Kaur; Pradeep Kumar; Archana Singh; Sanjay Kumar Singh Patel; Xiangkai Li; Yung-Hun Yang; Shashi Kant Bhatia; Saurabh Kulshrestha
Wastewater is not a liability, instead considered as a resource for microbial fermentation and value-added products. Most of the wastewater contains various nutrients like nitrates and phosphates apart from the organic constituents that favor microbial growth. Microalgae are unicellular aquatic organisms and are widely used for wastewater treatment. Various cultivation methods such as open, closed, and integrated have been reported for microalgal cultivation to treat wastewater and resource recovery simultaneously. Microalgal growth is affected by various factors such as sunlight, temperature, pH, and nutrients that affect the growth rate of microalgae. Microalgae can consume urea, phosphates, and metals such as magnesium, zinc, lead, cadmium, arsenic, etc. for their growth and reduces the biochemical oxygen demand (BOD). The microalgal biomass produced during the wastewater treatment can be further used to produce carbon-neutral products such as biofuel, feed, bio-fertilizer, bioplastic, and exopolysaccharides. Integration of wastewater treatment with microalgal bio-refinery not only solves the wastewater treatment problem but also generates revenue and supports a sustainable and circular bio-economy. The present review will highlight the current and advanced methods used to integrate microalgae for the complete reclamation of nutrients from industrial wastewater sources and their utilization for value-added compound production. Furthermore, pertaining challenges are briefly discussed along with the techno-economic analysis of current pilot-scale projects worldwide. © Copyright © 2021 Arora, Kaur, Kumar, Singh, Patel, Li, Yang, Bhatia and Kulshrestha.