Browsing by Author "Sanjay Yadav"

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A graphene-printed paper electrode for determination of H2O2in municipal wastewater during the COVID-19 pandemic
(Royal Society of Chemistry, 2022) Tushar Kant; Kamlesh Shrivas; Indrapal Karbhal; Monisha; Sanjay Yadav; Tikeshwari; Sushama Sahu; Yugal Kishor Mahipal; Vellaichamy Ganesan
Recently, hydrogen peroxide (H2O2) has been used as a disinfectant in sanitizers for cleaning hands, and solid surfaces of hospitals, offices and homes to prevent the spread of the COVID-19 virus. The effluents from domestic, hospital and municipal waste should be monitored for their H2O2 content to avoid the entry of this toxic pollutant into the ecosystem. Therefore, we developed a low-cost graphene (Gr)-printed paper electrode for determination of H2O2 using cyclic voltammetry (CV). An office inkjet-printer and Gr nano-ink stabilized with ethyl cellulose (EC) were used for the fabrication of printed paper electrodes (PPEs) to determine H2O2 quantitatively. A stable Gr-EC nano-ink (2%) with viscosity and surface tension values of 12 mPa S-1 and 35 mN M-1, respectively, was formulated to obtain conductive electrodes. A wide linear range (2 μM-25 mM) with a better limit of detection (0.28 μM) for the determination of H2O2 was obtained when the Gr-EC/PPE was used as a working electrode. Further, the Gr-EC/PPE was successfully employed for analysis of H2O2 in wastewater. The electrochemical determination of H2O2 using the Gr-EC/PPE as an electrode in CV is rapid, economical, flexible and eco-friendly when compared with previously reported methods. © 2022 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.
A new species of Podosphaera sect. Sphaerotheca subsect. Magnicellulatae from India and a key to all species reported on Fabaceae
(Magnolia Press, 2020) Sanjay Yadav; Sanjeet Kumar Verma; Raghvendra Singh; Vinay Kumar Singh; Prakash Kushwaha
Podosphaera thermopsidicola, a new sexual morph form of powdery mildew was discovered on living leaves of Thermopsis barbata Benth. (Fabaceae). It is described, illustrated and compared with other Podosphaera species reported on Fabaceae. The phylogeny of this species has been inferred from partial nuclear ribosomal 28S large subunit (LSU) and internal transcribed spacer (ITS) rDNA sequence data. On the basis of LSU, P. thermopsidicola represents characteristic feature of Podosphaera s. str. Phylogenetically, P. thermopsidicola is closer to P. erigerontis-canadensis but differs in having larger chasmothecia, longer appendages, thick-walled asci, smaller oculi and smaller ascospores. © 2020 Magnolia Press
A new species of Podosphaera sect. Sphaerotheca subsect. Sphaerotheca from India—first report of powdery mildew causing wilting and ultimately death of leaves of Filipendula vestita
(Magnolia Press, 2021) Sanjay Yadav; Sanjeet Kumar Verma; Raghvendra Singh
Podosphaera filipendulensis, a new teleomorph of powdery mildew was discovered on living leaves of Filipendula vestita (Rosaceae). It is the first species of powdery mildew that causes wilting and ultimately death of apical infected leaves of F. vestita. It is described, illustrated and compared with other Podosphaera species reported on Rosaceae and a key to all species reported on Rosaceae is provided. The phylogeny of this species has been inferred from internal transcribed spacer (ITS) rDNA sequence data. Phylogenetically, P. filipendulensis is close to P. ferruginea var. ferruginea and P. macularis but differs in having larger chasmothecia, smaller peridium cells, shorter, narrower and aseptate appendages, 8-spored asci and smaller ascospores. Copyright © 2021 Magnolia Press
Addition of three new lineages in Mycosphaerellaceae: Neoacervuloseptoria gen. nov., Neocercosporella gen. nov. and Neoramulariopsis gen. nov.
(Springer Science and Business Media Deutschland GmbH, 2023) Sanjay Yadav; Raghvendra Singh; Sanjeet Kumar Verma; Gargee Singh; Prakash Kushwaha
A colourless hyphomycetous fungus was found on living leaves of Peristrophe bicalyculata in India. Molecular phylogenetic analyses of several loci (LSU-RPB2-ITS) indicated a relationship with cercosporoid fungi (Mycosphaerellaceae). Since there is no other known lineage with similar morphology or DNA sequences, the new genus Neocercosporella is proposed with N. peristrophes comb. nov., based on Cercosporella peristrophes as type species. Pseudocercosporella andrographidis is recognized as new synonym. Similar ultrastructure of conidiogenous loci and hila confirm that N. peristrophes and P. andrographidis are conspecific. The conidiogenous loci are conical and have a small rim-like depression on the top encircling a small flat protuberant structure, which differs from other closely related members of the ramularioid complex. Neocercosporella and Cercosporella s. str. are paraphyletic as are several other genera in the Mycosphaerellaceae. Accordingly, we propose three new genera and four new combinations. The new genera are Neoacervuloseptoria gen. nov., Neocercosporella gen. nov. and Neoramulariopsis gen. nov. The new combinations are Neoacervuloseptoria fraxini comb. nov., Neocercosporella peristrophes comb. nov., Neoramulariopsis catenulata comb. nov. and Neoramulariopsis unguis-cati comb. nov. © 2023, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature.
An innovative technique for craniocaudal pedicle screw guidance: A technical note
(Lippincott Williams and Wilkins, 2018) Sanjay Yadav; Saurabh Singh
In spine surgery, pedicle screw instrumentation is being used very commonly. However, putting pedicle screws in the right place and direction needs the use of image intensifier in 2 orthogonal planes. This consumes valuable time while moving the image intensifier and operating table multiple times during the surgery and also excess radiation exposure. Here, we present a simple technique to apply surgical pearl to angulate the pedicle screws in cephalocaudal direction using simple instrument available on table. This may help in reducing the usage of image intensifier and expedite the procedure. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
Analysis of different entry portals for femoral nail with two different nail designs-straight nail versus lateral angulated nail - Does it make a difference?
(Elsevier B.V., 2019) Sanjay Yadav; Saurabh Singh; Anil Kumar Rai
[No abstract available]
Analysis of partial bundle anterior cruciate ligament tears- diagnosis and management with ACL augmentation
(Elsevier B.V., 2020) Sanjay Yadav; Saurabh Singh
Objectives: Partial ACL tears are increasingly recognized in young active patients. They can evolve into complete tears. Controversy exists regarding the need to spare intact ACL bundle as it has its advantages considering biomechanical strength, blood supply and proprioception. The current study determined the challenges in partial ACL tear management and assessed the functional outcomes. Methods: Twenty consecutive patients with partial ACL tears were studied. Inclusion criteria were: age 16–45yrs and patients operated for partial ACL tear. Exclusion criteria were: combined ACL-PCL injuries, associated collateral injuries, complete ACL tear, chondral defect or bony malalignment and patients with radiographic signs of arthritis. ‘Partial’ tear was defined as continuous fibers from native tibial ACL footprint to native femoral ACL footprint in arthroscopy. Clinical and radiological assessment was done to evaluate anteromedial(AM) or posterolateral(PL) bundle tears. We used the term “ACL-augmentation” without disturbing the intact bundle or preserving the intact fibers as much as possible. Functional scoring was done using Lysholm score. Standard post-operative protocols were followed. Statistical analysis was done using SPSS software. Results: Mean age of patients was 31.2 years. Physically active age group (<30yrs) included 62.5% patients. Males were 87.5%. Pain and instability were the presenting complaints in 75% and 70% respectively. Average duration of presentation since injury was 4.2 months. Sports activities were the most common mode of injury (45%) followed by road traffic accidents (37.5%). Anterior drawer test was positive in 40%, pivot shift in 35% and Lachman test was positive in 65%. On arthroscopy, 65% had AM bundle tears and 35% had PL bundle tears. The intact bundle was found lax in 13% cases. Associated meniscal tear was present in 28% patients. Stiff knee was the most common post-operative complaint. Preoperative Lysholm knee score of 74.5 improved to 87.7 at 12months (p < 0.001). Around 97.5% of the patients reported outcomes as good and fair. Conclusion: The treatment strategy needs to be individualized. The ACL augmentation needs more systematic and accurate placement of portals while sparing the intact ACL fibers. For AM bundle, tibial tunnel entry point is about 1–2 cm medial to tibial tuberosity. For PL bundle, it is about 3–4 cm medial to tibial tuberosity to protect the AM bundle. Long term studies with greater number of subjects are required. © 2019 Delhi Orthopedic Association
Analysis of vertical and horizontal offsets in displaced femoral neck fracture in elderly treated with indigenous bicentric bipolar hip device
(Elsevier B.V., 2021) Anil Kumar Rai; Sanjay Yadav; Vishal Verma; Saurabh Anand; Subhanshu Shekhar
Objectives: To analyze the effect of indigenous bicentric bipolar prosthesis on horizontal and vertical offsets in fracture neck of femur when compared to contralateral normal hip and to evaluate functional outcomes. We hypothesized that our non-modular bipolar device restores satisfactory offsets in such patients. Methods: All active elderly patients with displaced fracture NOF having contralateral normal hip were included. We used an indigenous bicentric bipolar hip-prosthesis, which is a non-modular single-piece device in all cases by lateral Hardinge approach. Postoperative radiograph AP view was taken in 15° internal rotation to decrease the effect of limb rotation on offset. CT scan was also used to evaluate offsets using ADW4.6 ADVANCED GE optima 128 slice software system. Subjects were followed for a minimum of 12 months postoperatively and functional outcome of effect of offsets change were evaluated by modified Harris Hip Score. Results: There is minimal difference in horizontal and vertical offset after bicentric bipolar hemi-replacement which is statistically insignificant supporting our hypothesis. The clinical outcomes were good to fair according to modified Harris Hip Score. The mean value of horizontal offset after our bipolar hemireplacement was 42.4 ± 2.04 mm and of normal hip was 41.8 ± 1.81 mm and P-value=0.08 in plain radiographs and value of horizontal offset in CT scan was 40.73 ± 0.27on bipolar side and 41.19 ± 0.77 on normal side. Vertical offset after bicentric bipolar was 32.67 ± 2.85 mm and vertical offset of normal hip was 32.53 ± 2.73 mm. Mean 9.77 ± 1.09 mm of calcar was preserved. Modified Harris Hip Score at 6 and 12 months postoperatively was 75.78 ± 4.16 and 79.53 ± 3.95 respectively. There was no incidence of hip dislocation. Conclusion: Our study data clearly demonstrates that vertical and horizontal offsets are effectively maintained by the indigenous bicentric hip device. There was insignificant change in offsets as compared to contralateral normal side due to its design modifications. Indigenous bicentric non-modular bipolar device offers an excellent option for femur neck fractures in elderly patients in resource constrained situations. It allows rapid rehabilitation due to reduced surgical time, minimal blood loss and early return to function and activities of daily living. © 2020 Delhi Orthopedic Association
Cercosporella bundelkhandae comb. nov. From India
(Mycotaxon Ltd, 2020) Raghvendra Singh; Sanjeet Kumar Verma; Sanjay Yadav; Shambhu Kumar
The new combination Cercosporella bundelkhandae ( Pseudocercosporella bundelkhandae) is proposed, based on critical morphological re-examination of the holotype specimen and fresh topotypic material and comparison with closely related species of cercosporoid hyphomycetes. The species was originally collected on living leaves of Tinospora sinensis from Jhansi, Utttar Pradesh, India. © 2020 Mycotaxon Ltd. All rights reserved.
Comparative analysis of transforaminal lumbar interbody fusion versus posterolateral instrumented fusion in degenerative lumbar spine disorders
(SAGE Publications Inc., 2020) Sanjay Yadav; Saurabh Singh; Raj Kumar Arya; Alok Kumar; Ishan Kumar; Abhinav Jha
Objectives: Spinal fusion is an effective treatment for degenerative lumbar spine; however, conflicting results exist regarding the best procedure. This study compares the clinical and radiological outcomes of transforaminal lumbar interbody fusion (TLIF) versus instrumented posterolateral fusion (PLF) in patients of degenerative lumbar spine disorders. Methods: Of the total 37 patients, 16 patients were operated with TLIF and 21 were operated with instrumented PLF with bone grafting. Duration of the study was from June 2017 to June 2019. Patients fulfilling the inclusion criteria were included in the study. Inclusion criteria were (1) age of patient ranging from 18 years to 70 years, (2) involvement of single level, (3) diagnosis of degenerative spine disease, and (4) minimum follow-up of 1 year. Radiographic parameters such as slippage of vertebrae, anterior and posterior disc heights, local disc lordosis, T12–S1 angle were measured, and fusion were assessed; comparison between preoperative and postoperative parameters was also done. Clinical outcome score was obtained using visual analog scale (VAS) and Oswestry disability index (ODI). Statistical analysis was done using SPSS software. Results: No significant difference was found in ODI and VAS between TLIF and PLF. Restoration of disc height and improvement of local disc lordosis was better in the TLIF group than in the PLF group. The fusion rate was 87.5% in the TLIF group and 81% in the instrumented PLF group. Amount of blood loss was slightly higher in the TLIF group (319.69 ± 53.8 mL) than in the instrumented PLF group (261.19 ± 34.9 mL). Operating time was also slightly higher in TLIF (133 ± 6.02 min) than in instrumented PLF (90.71 ± 6.3 min). Conclusion: TLIF is superior to instrumented PLF in terms of restoration of anterior and posterior disc heights and improvement in local disc lordosis and higher fusion rate, however it requires greater surgical expertise and more experience. Because of anterior cage support, early weight-bearing mobilization can be allowed in the TLIF group compared to the PLF group. Surgical time and blood loss were slightly higher in cases of TLIF than instrumented PLF. © The Author(s) 2020.
Comparison of intra-articular lumbar facet joint injection of platelet-rich plasma and steroid in the treatment of chronic low back pain: A prospective study
(SAGE Publications Inc., 2023) Chandan Singh; Sanjay Yadav; Sandeep Loha; Shashi Prakash; Anil Kumar Paswan
Study design: Prospective randomized clinical study. Purpose: To compare the effectiveness and safety of intra-articular platelet-rich plasma (PCP) and steroid along with radiofrequency ablation (RFA) in the treatment of chronic low back pain (LBP) due to facet joint arthropathy. Overview of literature: Facet joint pathology is an important cause of LBP—15–30% of all LBP cases. Lumbar intra-articular PRP is a relatively new method in the treatment of LBP. PRP stimulates the cells involved in regeneration. Hence, it seems a suitable option for the treatment of lumbar facet joint syndrome. Methods: We evaluated the efficacy and safety of facet joint injections in LBP secondary to facet joint arthropathy. Chronic LBP for ≥3 months (visual analogue scale (VAS) > 4), failed conservative treatment, no neurological deficit, unilateral facet joint pain, focal tenderness with hyperextension pain, and relief by diagnostic medial branch block were included. Patients were randomly allocated to Group S: Steroid (Triamcinolone) + RFA or Group P: PRP + RFA or Group R: 0.9% saline + RFA as control. Demographic, clinico-radiological, and outcome parameters were recorded till 6 months. Data were analyzed using SPSS and p < 0.05 was considered significant. Results: We studied 45 patients (n = 15 in each group) in the final analysis. Mean age was 45.7 ± 13.6 years and 60% were females in all groups. VAS decreased to 1.6 ± 0.8 (Group S) and 3.2 ± 0.8 (Group P) on day 1 (p < 0.05). At 3 and 6 months, VAS reduced more in Group P (0.47 ± 0.5; 0.07 ± 0.2) versus Group S (2.53 ± 0.5; 3.07 ± 0.2) (p < 0.001). Mean Oswestry Disability Index (ODI) score at baseline was 72.8 ± 7.6 (all groups). At 1 month, Group S (17.2 ± 3.2) showed better improvement than Group P (23.2 ± 3.1) (p < 0.05). At 6 months, Group P (8.9 ± 1.2) had more decrease in ODI than Group S (29.0 ± 2.1) (p < 0.001). NSAIDs usage and Patient Satisfaction Score (PSS) were significantly better at 6 months in Group P than Group S (p < 0.01; p < 0.05, respectively). Conclusion: Both PRP and corticosteroid injections were determined to be effective and safe for the treatment of lumbar facet joint syndrome after 6 months of follow-up. However, autologous PRP may be a superior treatment option for longer efficacy. © The Author(s) 2023.
Correction to: Plasma fibrinogen is a natural deterrent to amyloid β-induced platelet activation and neuronal toxicity (Molecular Medicine (2016) 22 (224-232) DOI: 10.2119/molmed.2016.00003)
(BioMed Central Ltd., 2019) Vijay K. Sonkar; Paresh P. Kulkarni; Susheel N. Chaurasia; Ayusman Dash; Abhishek Jauhari; Devendra Parmar; Sanjay Yadav; Debabrata Dash
Following publication of the original article [1], the author reported an error in Figure 1. The correct version of Figure 1 is as follows: (Figure Presented). © 2019 The Author(s).
Distocercospora curvulata sp. nov. from northern India
(Mycotaxon Ltd, 2022) Sanjeet Kumar Verma; Sanjay Yadav; Raghvendra Singh
A new species of asexual foliicolous fungus, Distocercospora curvulata, discovered on living leaves of Causonis trifolia from Uttarakhand, India, is described and illustrated. © 2022 Mycotaxon Ltd. All rights reserved.
Enhanced Osteogenesis by Molybdenum Disulfide Nanosheet Reinforced Hydroxyapatite Nanocomposite Scaffolds
(American Chemical Society, 2019) Umakant Yadav; Himanshu Mishra; Vimal Singh; Sunayana Kashyap; Anchal Srivastava; Sanjay Yadav; Preeti S. Saxena
The advances in the arena of biomedical engineering enable us to fabricate novel biomaterials that provide a suitable platform for rapid bone regeneration. Herein, we have investigated the in vitro and in vivo osteogenic differentiation, proliferation, and bone regeneration capability of molybdenum disulfide nanosheets (MoS2NSs) reinforced HAP nanocomposite scaffolds. The MG-63 cells were incubated with HAP and HAP/MoS2NSs nanocomposite and followed for various cellular activities. The cells incubated with HAP@2 shows higher cell adhesion, cell proliferation, and alkaline phosphatase activity (ALP) in contrast to HAP. The in vivo and in vitro results of the increased ALP level confirm that HAP@2 promotes osteogenic differentiation. This improved osteogenesis was validated with upregulation of osteogenic marker viz. transcription factor, RUNX-2 (∼34 fold), collagen-1 (∼15 fold), osteopontin (∼11 fold), osteocalcin (∼20 fold), and bone morphogenetic protein-2 (∼12 fold) after 12 week postimplantation in comparison to drilled. The X-ray imaging demonstrates that HAP@2 implants promote rapid osteogenesis and bioresorbability than HAP and drilled. The outcomes of the present study provide a promising tool for the regeneration of bone deformities, without using any external growth factor. © 2019 American Chemical Society.
Epoxyazadiradione exhibit activities in head and neck squamous cell carcinoma by targeting multiple pathways
(Springer, 2020) Vipin Rai; Sushil Kumar Aggarwal; Sumit Singh Verma; Nikee Awasthee; Anupam Dhasmana; Sadhna Aggarwal; Satya N. Das; Mangalam S. Nair; Sanjay Yadav; Subash C. Gupta
The head and neck squamous cell carcinoma (HNSCC) constitute about 90% of all head and neck cancers. HNSCC falls in the top 10 cancers in men globally. Epoxyazadiradione (EPA) and Azadiradione (AZA) are the limonoids derived from the medicinal plant Azadirachta indica (popularly known as Neem). Whether or not the limonoids exhibit activities against HNSCC and the associated mechanism remains elusive. Herein, we demonstrate that EPA exhibits stronger activity in HNSCC in comparison to AZA. The limonoids obeyed the Lipinski’s rule of 5. EPA exhibited activities in a variety of HNSCC lines like suppression of the proliferation and the induction of apoptosis. The limonoid suppressed the level of proteins associated with anti-apoptosis (survivin, Bcl-2, Bcl-xL), proliferation (cyclin D1), and invasion (MMP-9). Further, the expression of proapoptotic Bax and caspase-9 cleavage was induced by the limonoid. Exposure of EPA induced reactive oxygen species (ROS) generation in the FaDu cells. N-acetyl-L-cysteine (ROS scavenger) abrogated the down-regulation of tumorigenic proteins caused by EPA exposure. EPA induced NOX-5 while suppressing the expression of programmed death-ligand 1 (PD-L1). Further, hydrogen peroxide induced NF-κB-p65 nuclear translocation and EPA inhibited the translocation. Finally, EPA modulated the expression of lncRNAs in HNSCC lines. Overall, these results have shown that EPA exhibit activities against HNSCC by targeting multiple cancer related signalling molecules. Currently, we are evaluating the efficacy of this molecule in mice models. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
MicroRNA-29b-3p degenerates terminally differentiated dopaminergic SH-SY5Y cells by perturbation of mitochondrial functions
(John Wiley and Sons Inc, 2024) Sana Sarkar; Anuj Pandey; Sanjeev Kumar Yadav; Pragati Raghuwanshi; Mohammed Haris Siddiqui; Saripella Srikrishna; Aditya Bhushan Pant; Sanjay Yadav
Mitochondrial dysfunction is the main cause of gradual deterioration of structure and function of neuronal cells, eventually resulting in neurodegeneration. Studies have revealed a complex interrelationship between neurotoxicant exposure, mitochondrial dysfunction, and neurodegenerative diseases. Alteration in the expression of microRNAs (miRNAs) has also been linked with disruption in mitochondrial homeostasis and bioenergetics. In our recent research (Cellular and Molecular Neurobiology (2023) https://doi.org/10.1007/s10571-023-01362-4), we have identified miR-29b-3p as one of the most significantly up-regulated miRNAs in the blood of Parkinson's patients. The findings of the present study revealed that neurotoxicants of two different natures, that is, arsenic or rotenone, dramatically increased miR-29b-3p expression (18.63-fold and 12.85-fold, respectively) in differentiated dopaminergic SH-SY5Y cells. This dysregulation of miR-29b-3p intricately modulated mitochondrial morphology, induced oxidative stress, and perturbed mitochondrial membrane potential, collectively contributing to the degeneration of dopaminergic cells. Additionally, using assays for mitochondrial bioenergetics in live and differentiated SH-SY5Y cells, a reduction in oxygen consumption rate (OCR), maximal respiration, basal respiration, and non-mitochondrial respiration was observed in cells transfected with mimics of miR-29b-3p. Inhibition of miR-29b-3p by transfecting inhibitor of miR-29b-3p prior to exposure to neurotoxicants significantly restored OCR and other respiration parameters. Furthermore, we observed that induction of miR-29b-3p activates neuronal apoptosis via sirtuin-1(SIRT-1)/YinYang-1(YY-1)/peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α)-regulated Bcl-2 interacting protein 3-like-dependent mechanism. Collectively, our studies have shown the role of miR-29b-3p in dysregulation of mitochondrial bioenergetics during degeneration of dopaminergic neurons via regulating SIRT-1/YY-1/PGC-1α axis. (Figure presented.) © 2024 International Society for Neurochemistry.
Molecular phylogeny of Aplosporella abexaminans: a novel species revealing the second report of sexual-asexual connection in Aplosporellaceae (Botryosphaeriales) from India
(Magnolia Press, 2021) Sanjeet Kumar Verma; Sanjay Yadav; Raghvendra Singh
Aplosporella abexaminans, a new sexual morph fungus belongs to family Aplosporellaceae, was discovered on the bark of stem of Murraya koenigii (Rutaceae) and identified by morphological characteristics and analysis of combined ITS and LSU sequence data. This is the second report of a sexual morph with molecular evidence for this genus and the second record of conidiogenesis and chlamydospores associated with the asexual state of this family. It is characterized by its larger ascostromata, locules without ostioles, thinner locule peridium, two to multi-layered, larger asci, surrounded by an additional hyaline sac like structure, larger and hyaline to light olivaceous ascospores, asexual state without conidiomata formation, conidiogenous cells light brown to brown, conidia brown without granular content, smooth and presence of chlamydospores. The sexual morph of Aplosporella abexaminans resembles Bagnisiella and the asexual morph resembles Aplosporella, thus proving the sexual-asexual connection for the second time for this family. © 2021 Magnolia Press.
Morphology and phylogeny of a new species, Pseudocercospora haldinae (Mycosphaerellaceae) on Haldina cordifolia from India
(Magnolia Press, 2021) Sanjay Yadav; Sanjeet Kumar Verma; Vinay Kumar Singh; Raghvendra Singh; Archana Singh; Shambhu Kumar
Pseudocercospora haldinae, a new anamorphic foliicolous hyphomycetous fungus discovered on living leaves of Haldina cordifolia (Rubiaceae) is taxonomically described and illustrated. This species is compared with closely related species of Pseudocercospora and other dematiaceous cercosporoid forms reported on the same host genus. The phylogeny of this species has been inferred from partial nuclear ribosomal 28S large subunit (LSU) and complete internal transcribed spacer (ITS) rDNA sequence data. On the basis of LSU, P. haldinae represents characteristic features of Pseudocercospora s. str. and did not form red crystals when cultivated on agar media. © 2021 Magnolia Press. © 2021 Magnolia Press. All rights reserved.
Morphology and phylogeny of Teratoramularia rumicis—a new foliar pathogen of Rumex crispus from India and diversity of Ramularioid complex on Rumex spp.
(Magnolia Press, 2021) Sanjeet Kumar Verma; Prakash Kushwaha; Sanjay Yadav; Raghvendra Singh
Teratoramularia rumicis, a new hyaline anamorphic foliicolous hyphomycetous fungus was discovered on living leaves of medicinally important plant Rumex crispus (Polygonaceae) is described and illustrated. It is an obligate foliar pathogen that causes silvery white foliar disease. It is the first report of Teratoramularia from India. This species is compared with closely related species of Teratoramularia and species of Ramularioid complex along with dematiaceous cercosporoid forms reported on the same host genus and family. The phylogeny of this species has been inferred from combined 28S nrRNA gene (LSU) and internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon. Copyright © 2021 Magnolia Press
Neokamalomyces indicus gen. nov., sp. nov. (Mycosphaerellaceae)—a Septoria-like genus from India
(Magnolia Press, 2022) Sanjay Yadav; Sanjeet Kumar Verma; Raghvendra Singh; Vinay Kumar Singh; Balmukund Chaurasia; Paras Nath Singh; Shambhu Kumar
A new hyaline coelomycetous fungus discovered on living leaves of Ficus benghalensis (Moraceae) is described and illustrated. Morphologically, it is similar to Septoria or septoria-like genus, but based on cultural characteristics and multigene (LSU-RPB2-ITS) phylogenetic analysis this strain represents an additional lineage in Mycosphaerellaceae. Hence, a new genus and species, Neokamalomyces indicus is proposed. Copyright © 2022 Magnolia Press.