Browsing by Author "Sanjeev Kumar Maurya"

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Expression of procaspase 3 and activated caspase 3 and its relevance in hormone-responsive gallbladder carcinoma chemotherapy
(2013) Sanjeev Kumar Maurya; Mallika Tewari; Bechan Sharma; Hari Shanker Shukla
Background/Aims: The higher incidence of gallbladder cancer (GBC) in females has been accredited to the involvement of hormones. The clinical implications of sex hormone receptors in GBC are well established. Cysteine proteases (such as caspase-3-9, etc.) are known to play a central role in the apoptotic pathway. Of these, the downstream enzyme caspase-3 is often activated in the apoptotic path-way. The aim of this work was to examine the status of apoptosis (which directly correlated with the level of active caspase-3) in hormone-responsive GBC. Methods: We used 10 androgen receptor (AR)-positive, 14 estrogen receptor (ER)-positive, 12 HER/neu-positive, eight triple positive, and 10 triple negative malignant GBC human tissue samples. We isolated the total cellular protein from tumor tissues and carried out Western blotting using antipro-caspase-3 and anti-activated caspase-3 antibodies. Results: ER and HER/neu-positive GBC exhibited high caspase-3 activity and low procaspase-3 activity, whereas AR-positive GBC showed no significant level of apoptosis. We also evaluated the apoptosis status of triple positive GBC and triple negative GBC, and found significant apoptosis in triple positive GBC. Conclusions: The results indicate that ER and HER/neu-positive GBCs had active apoptosis, whereas AR-positive GBC was highly resistant to apoptosis. © 2013 The Korean Association of Internal Medicine.
Expression pattern of tumor endothelial marker 8 protein in gallbladder carcinomas
(Asian Pacific Organization for Cancer Prevention, 2011) Sanjeev Kumar Maurya; Mallika Tewari; Mohan Kumar; Mahendra Kumar Thakur; Hari Shanker Shukla
Tumor endothelial marker 8 protein (TEM8) is highly specific to tumor angiogenesis and is not required for normal adult angiogenesis and hence might prove to be a target for anti-angiogenic therapies in the future. We here evaluated protein and gene expression patterns in human endothelial cells of benign gallbladder - gallstone diseases (GSDs) and gallbladder carcinomas (GBCs) using immunostaining, immunofluorescence and western blotting techniques. Subjects comprised 175 GBC patients, 38 males and 137 females, aged 30-85 years (mean age 50.3±13.4 years) and twenty with GSDs, aged 30-75 years, (51.4±10.0 years) for comparison (male 4/20 and females 16/20). TEM8 protein expression increased significantly (p<0.0001) with increasing stage of GBC and was mostly limited to endothelial cells, although there was no significant change with the grade. Interestingly, only 80-85 kDa and 60 kDa isoforms of TEM8 increased significantly whereas 45 kDa isoform was absent in GBCs. Conclusions- These results suggest that TEM8 plays an unknown important biological role to promote tumor angiogenesis in GBC.
Genomic profiling of breast cancer
(2009) Anjita Pandey; Alok Kumar Singh; Sanjeev Kumar Maurya; Rajani Rai; Mallika Tewari; Mohan Kumar; Hari S. Shukla
Genome study provides significant changes in the advancement of molecular diagnosis and treatment in Breast cancer. Several recent critical advances and high-throughput techniques identified the genomic trouble and dramatically accelerated the pace of research in preventing and curing this malignancy. Tumor-suppressor genes, Proto-oncogenes, DNA-repair genes, Carcinogen-metabolism genes are critically involved in progression of breast cancer. We reviewed imperative finding in breast genetics, ongoing work to segregate further susceptible genes, and preliminary studies on molecular profiling. © 2009 Wiley-Liss, Inc.
Human tumour tissue bank: An essential requirement for Indian tumour biology research [6]
(2006) Sanjeev Kumar Maurya
[No abstract available]