Browsing by Author "Sateesh Chandra Gupta"

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Engineered an ultrasmall curcumin oral nanoformulation restores intestinal integrity and gut microbiota dysbiosis
(Elsevier B.V., 2025) Vivek Sharma; Prateeksha Prateeksha; Balwant Singh Paliya; Sateesh Chandra Gupta; Sarvendra Singh; Anand Anunay; Sushil Agrahari; Shailendra P. Singh; Chandana Venketswara Rao; Saroj Kanta Barik; Brahma Nand Singh
Antibiotic therapy for bacterial infections often disrupts gut microbiota (GM) and intestinal integrity, inducing chronic inflammation and inflammatory bowel diseases. An effective therapeutic intervention is urgently needed to alleviate the aforementioned adverse effects of antibiotics. Curcumin (CUR) reveals great potential to restore intestinal integrity and GM dysbiosis due to its strong antiinflammatory and prebiotic effects. However, the weak solubility and stability of CUR result in limited bioavailability and a short half-life, which restricts its clinical uses. An ultra-small CUR oral nanoformulation was created using a carboxylated galactomannan (cGM), facilitated by hydrogen bonding between the phenolic hydroxyl groups of CUR and the carboxyl groups present on cGM. The developed nanoformulation increased CUR stability both in vitro and in vivo, extended its retention period in the gastrointestinal tract, and enhanced its permeability across the mucus layer and intestinal epithelium to improve oral bioavailability of CUR. The nanoformulation attained notable therapeutic efficacy in restoring intestinal epithelial barrier dysfunction and GM dysbiosis, as validated in an antibiotic-induced in vivo model. This research highlights the benefits of cGM in creating a very stable and ultrasmall nanoformulation for CUR, offering a promising oral nanoplatform for the delivery of CUR. © 2025
Growth, phytochemical and gene expression changes related to the secondary metabolite synthesis of Solanum viarum Dunal
(Elsevier B.V., 2021) Preeti Patel; Archana Prasad; Sateesh Chandra Gupta; Abhishek Niranjan; Alok Lehri; Satya Shila Singh; Pratibha Misra; Debasis Chakrabarty
Solanum viarum Dunal, is an important medicinal plant widely used as a source of raw material for the steroidal drug industry. Out of various steroidal precursors, solasodine, an analogue of diosgenin is the most important source of raw material for the synthesis of steroidal drugs. In the present study, growth and phytochemical variations in different tissue (leaves, stem, roots, and berries) of two contrasting genotypes, prickled and prickleless Solanum viarum were evaluated under in vitro and field conditions. Significant variation in growth, yield and contents of glycoalkaloids, phenolics, and flavonoids were evident between two genotypes under in vitro and field conditions. Prickleless genotypes showed improved growth parameters both under in vitro and field conditions, that helps in contributing to higher yield over prickled plants. Tissue-specific chemical analysis revealed that the leaves and roots of prickleless plants serve as a better repertoire for bioactive phytomolecules. The vegetative parts of in vitro grown 50 days old prickleless plants showed comparable/higher metabolite content than 7–8 month old field-grown plants. Hence it can be used as an alternative production platform for further upscaling, elicitation, and precursor feeding targets that enhances its commercial utility. The expression analysis of seven genes involved in the regulation of important metabolite biosynthesis in both prickled and prickleless genotype of Solanum viarum was also examined. The diverse metabolite profiles are correlated with variations in gene expression profiles. This study provides information about the key metabolites and its biosynthetic pathway genes, which could be useful for the selection of an improved prickleless genotype (“Nishkantak”) of Solanum viarum. © 2021 Elsevier B.V.