Browsing by Author "Satyajit Pradhan"

Now showing 1 - 20 of 41

5-fluorouracil induces defects in platelet function
(Carfax Publishing Company, 1999) Anand Kumar; Rajeev Kumar; Amitabh Sandilium; Jyoti Shukla; Satyajit Pradhan
Platelet factor-3 (PF3) availability and platelet aggregation to ADP (4 μmol/l) and adrenaline (1.2 μmol/l) were evaluated in patients being treated with 5-fluorouracil (5-FU) for gastrointestinal malignancy. It produced a significant reduction in platelet aggregation and platelet factor-3 (PF3) availability (P < 0.001) without being associated with thrombocytopenia. The changes in platelet aggregation occurred during the first week of chemotherapy and continued with subsequent courses. This acquired abnormality may be responsible for a haemorrhagic diathesis even without obvious thrombocytopenia. Study of platelet function is important and may be considered to assess the haematological toxicity in patients being treated with systemic 5-FU therapy.
A comparison between revised Manchester Point A and ICRU-89–recommended Point A definition absorbed-dose reporting using CT images in intracavitary brachytherapy for patients with cervical carcinoma
(Elsevier Inc., 2021) Ankur Mourya; Sunil Choudhary; Uday Pratap Shahi; Neeraj Sharma; Himani Gautam; Ganeshkumar Patel; Satyajit Pradhan; Lalit Mohan Aggarwal
Purpose: This study is a comparison between revised Manchester Point A and International Commission on Radiation Units and measurements (ICRU) 89 report–recommended Point A absorbed-dose reporting in intracavitary brachytherapy for patients with cervical carcinoma. Methods and Materials: The retrospective dosimetric study is based on the data of 32 patients with cervical carcinoma treated with high-dose-rate brachytherapy. Patients received 21 Gy in three fractions (7.0 Gy X three fractions) to Point A (Aflange, revised Manchester definition). All the patients were replanned with a new Point A (Aicru89) defined on CT images as per the American Brachytherapy Society/ICRU-89. The data collected were compared with the data obtained from Point A (Aflange). Results: When using the Aflange plan normalization method, the mean dose of 0.1 cc, 1 cc, and 2 cc bladder volumes was 820.79 ± 207.47 cGy, 654.66 ± 152.69 cGy, and 588.91 ± 136.35 cGy, respectively. Likewise, when using the ICRU-89 Point Aicru89 normalization method, the mean dose of 0.1 cc, 1 cc, and 2 cc bladder volumes was 869.30 ± 224.67 cGy, 693.24 ± 166.20 cGy, and 616.61 ± 150.32 cGy, respectively. For the rectum, Point Aflange normalization plans, the mean dose of 0.1 cc, 1 cc, and 2 cc volumes was 589.37 ± 163.26 cGy, 487.51 ± 126.03 cGy, and 442.70 ± 111.43 cGy, respectively. Likewise, using the Aicru89 plan, the mean 0.1 cc, 1 cc, and 2 cc rectum volume was 625.07 ± 171.31 cGy, 517.50 ± 131.05 cGy, 464.94 ± 121.81 cGy, respectively. The statistical mean difference of Total Reference Air Kerma rate, V100 (cc), bladder, rectum and sigmoid, was found significant. Conclusions: It has been found that the position of revised Manchester (Aflange) and ICRU-89 Point A does not match on CT images/radiograph, which resulted in variation in doses to the tumor, V100 (cc), organ at risk, and Total Reference Air Kerma. © 2021 American Brachytherapy Society
A comparison of six fractions per week chemoradiation versus five fractions per week of conventional chemoradiation in carcinoma cervix: A prospective controlled study
(Wolters Kluwer Medknow Publications, 2019) Deepak Kumar; Satyajit Pradhan; Sunil Choudhary; Lalit Aggarwal; Avipsa Das; Sovan Sarangdhar; Prashant Kaser; Satish Dewangan
Aims: The standard of care for carcinoma cervix stage IB2-IVA is five fractions per week of radiotherapy (RT) with concurrent cisplatin. We compared the standard treatment with six fractions per week of RT with concurrent Cisplatin to see whether the later had improved survival outcomes with comparable toxicities. Settings and Design: 46 patients of carcinoma cervix with stage IB2-IVAwere randomized into two arms. Materials and Methods: Study arm: 46 Gy/23 fractions/26 days, 6 fractions/week with injection CDDP 40 mg/m2 and Control arm: 46 Gy/23 fractions/31 days, 5 fractions/week with injection Cisplatin 40mg/m2. Patients in both the arms received LDR brachytherapy to a dose of 29 Gy at point A. Statistical Analysis Used: The primary end points were disease-free survival (DFS) and overall survival (OS). Compliance to treatment and treatment toxicities were the secondary end points. P value ≤0.05 were considered significant. Results: The study was carried out during June, 2014-April, 2015. Statistical analysis was done in May, 2019. Of 46 patients, 39 patients completed the treatment. The study and control arms had 17 and 22 patients, respectively. Median follow-up period is 45 months (range: 1-54 months). 3-year DFS rates and OS was 69.5% vs. 72.7% (P = 0.73) and 63% vs. 68% (P = 0.45) in study and in control arm, respectively. There was no significant difference in acute and late radiation toxicities between two arms. Conclusion: Chemoradiotherapy with six fractions per week seems feasible and equally efficacious in terms of survival outcomes and toxicity profile. Further prospective randomized controlled study is required to prove the merit of altered fractionation with concurrent cisplatin. © 2019 Journal of Cancer Research and Therapeutics - Published by Wolters Kluwer - Medknow.
A mathematical model to predict the different isodose volumes using TRAK value in HDR intracavitary brachytherapy for revised Manchester and ICRU-89 based Point A plans using computer tomography images
(Wolters Kluwer Medknow Publications, 2022) Ankur Mourya; Sunil Choudhary; Neeraj Sharma; Uday Pratap Shahi; Gaganpreet Singh; Satyajit Pradhan; Lalit Mohan Aggarwal
Purpose: To find out the simple relationship between Total Reference Air Kerma (TRAK) and various isodose volumes. Calculated isodose volumes were compared with experimental data for revised Manchester and International Commission on Radiation Units and measurements (ICRU)-89 Point A-based treatment plans. The accuracy of the formula was compared with the results of other relationships available in the literature. Materials and Methods: Dosimetric data from 62 intracavitary brachytherapy (ICBT) treatment plans of 31 patients with cervical cancer were studied. Each patient had treatment plans normalized to revised Manchester and ICRU-89 Points A (A flange and A icru89). For each treatment plan, TRAK values, V 350, V 700, V 1050, and V 1400 were obtained. The modeling curve was plotted between Isodose volume (V d) and the ratio of d/TRAK obtained from A flange plans to get a mathematical relation. The results of this formula were compared with the experimental data and outcomes of other formulas available in the literature. A paired-sample t-Test was performed to assess the statistical significance. Results: In the case of revised Manchester-based A flange normalization plans, the mean isodose volume of V 350, V 700, V 1050, and V 1400 were 285.98 ± 32.3 cm 3, 101.96 ± 10.63 cm 3, 52.71 ± 4.72 cm 3, and 31.44 ± 2.33 cm 3 respectively. Likewise, for ICRU-89 based A icru89 normalization plans, the mean isodose volumes of V 350, V 700, V 1050, and V 1400 were 304.11 ± 26.17 cm 3, 108.88 ± 8.29 cm 3, 56.62 ± 3.69 cm 3 and 34 ± 2.23 cm 3 respectively. The mean difference was significant. The Mathematical relationship developed was [INLINE:1]. No correlation was found between TRAK and D 0.1cm 3,D 2cm 3 for organs at risk. Conclusions: The developed formula calculated isodose volumes within the accuracy of ± 3% in ICBT plans. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
A Prospective Study to Evaluate the Feasibility and Outcome of Completing Concurrent Chemo-radiation and Brachytherapy Within 4 Weeks Using Two Different Chemotherapy Regimens in Carcinoma Cervix
(Springer, 2018) Sovan Sarang Dhar; Uday Pratap Shahi; Tejbali Singh; Anupam Kumar Asthana; Satyajit Pradhan; Lalit Mohan Aggarwal; Sunil Choudhary; Abhijit Mandal; Deepak Kumar
Purpose: A prospective study was performed to assess the feasibility and outcome of completing concurrent chemo-radiotherapy (CCRT) in ≤ 4 weeks in cervical cancer patients using combination of paclitaxel and cisplatin as weekly regimen versus weekly cisplatin as concurrent chemotherapy. Methods: Forty patients of mostly locally advanced stage were prospectively allocated to receive either weekly cisplatin (40 mg/m2) [arm A] or weekly paclitaxel (50 mg/m2) and cisplatin (30 mg/m2) [arm B]. External Beam Radiotherapy [EBRT] in both arms given was 45 Gy/20 fractions/5 fractions per week (using conventional technique, Co60 teletherapy), intracavitary radiotherapy 30 Gy to point A (using LDR in a single setting) in between EBRT fractions, starting after completion of 10 fractions of EBRT. Results: Treatment of 65% of arm B and 70% of arm A patients could be completed by 4 weeks. At a median follow-up of 24 months, 85% patients in each arm had complete loco-regional control, on intention to treat analysis. Thirty-month survival in the respective arms were 69 and 72.2%. Grade 3 acute toxicity varied between 10 and 15% in both arms. Grade-5 acute haematological toxicity was found in one patient (5%) of each arm. Difference between the two arms was statistically not significant for response, survival or toxicity. Conclusion: Completing CCRT by 4 weeks is feasible with acceptable acute toxicity and encouraging outcome for carcinoma cervix. Concurrent weekly cisplatin + paclitaxel do not improve loco-regional control or survival as compared to weekly cisplatin alone at the cost of similar toxicity. Large sample study and long follow-up are needed to establish the same and identify late toxicities. © 2018, Association of Gynecologic Oncologists of India.
A study for evaluating clinical relevance of circulating cell‑free DNA in cervical cancer
(Wolters Kluwer Medknow Publications, 2022) Anju Shrivastava; Garima Singh; Kumud Tiwari; Surendra Pratap Mishra; Satyajit Pradhan; Lalit Mohan Agarwal; Samarendra Kumar Singh
Introduction: Recent techniques available for the detection of cervical cancer (CC) are highly invasive and costly, which makes it a rate‑limiting step toward early diagnosis of this fatal disease. Evaluation of circulating cell‑free DNA (ccfDNA) through liquid biopsy is a minimally invasive and cost‑effective method that may serve as a unique tumor marker for early detection, treatment monitoring, the status of residual disease, and distant tumor metastasis in CC patients. Materials and Methods: In this study, initially, ccfDNA was measured in serum samples from 11 histopathologically proven cervix carcinoma patients and 8 controls. On successful screening, it was further extended to 2 more patients with a series of serum samples extracted at 3 different phases of the concurrent chemoradiotherapy (i.e., before, during, and after 6 months of follow‑up). Results: Agarose gel electrophoresis profile for ccfDNA of CC patients showed that of 11 patients, 4 patients had a comparatively higher tumor burden (ccfDNA) than the other 7 patients. Notably, during concurrent chemoradiotherapy, ccfDNA load disappeared and, after 6 months of follow‑up, appeared back due to distant metastasis. Conclusion: Hence, we propose that this method could be an affordable and reliable way to diagnose/screen CC. © 2020 Journal of Cancer Research and Therapeutics.
Addition of Etoricoxib During Concurrent Chemo-radiation of Cervical Cancer Patients Could Result in Faster Resolution of Gross Disease: A Prospective Single-Institution Study
(Springer, 2020) Sovan Sarang Dhar; Uday Pratap Shahi; Deepak Kumar; Ritusha Mishra; Prashant Kaser; Satish Dewangan; Abhijit Mandal; Sunil Choudhary; Lalit Mohan Aggarwal; Anupam Kumar Asthana; Satyajit Pradhan
Objective: A prospective study was conducted to assess the effect of adding COX-2 inhibitor Etoricoxib during concurrent chemo-radiotherapy schedule of cervical cancer patients on tumour response and acute toxicities. Materials and Methods: Forty patients of carcinoma cervix [mostly locally advanced] were treated using external beam radiotherapy (EBRT) [telecobalt, 45 Gy/20F/5F per week] concurrent with weekly cisplatin- or cisplatin + paclitaxel-based chemotherapy. Low-dose-rate (LDR) intracavitary brachytherapy (ICBT) 30 Gy to point A was delivered in between EBRT fractions in a single setting. Patients were prospectively allocated either to receive Etoricoxib 90 mg OD during the entire course of chemo-radiation [arm A] or not [arm B]. Weekly assessment with clinical evaluation and routine blood tests were done during the course of treatment, with pre-ICBT clinical evaluation taken into consideration for disease response comparison between arms. Results: When evaluated clinically before intracavitary brachytherapy procedure, the gross disease was found to have regressed more in the arm receiving Etoricoxib [p = 0.042]. Acute grade-3 toxicities ranged between 5 and 15% for patients who received Etoricoxib and 10–15% for those who did not. Difference in toxicities was not statistically significant. Conclusion: Addition of COX-2 inhibitor [Etoricoxib] during concurrent chemo-radiation results in a faster response of the primary disease in locally advanced cervical cancer patients, without a significant difference in acute toxicities. © 2019, Association of Gynecologic Oncologists of India.
An assessment of serum oxidative stress and antioxidant parameters in patients undergoing treatment for cervical cancer
(Elsevier Inc., 2021) Anju Shrivastava; Surendra Pratap Mishra; Satyajit Pradhan; Sunil Choudhary; Saurav Singla; Kulsoom Zahra; Lalit Mohan Aggarwal
Objectives: Oxidative stress and antioxidants are involved in all aspects of cervical cancer. The present study evaluated serum levels of oxidative stress and antioxidant biomarkers in cervical cancer patients and healthy controls. Moreover, the effect of Concurrent chemoradiotherapy (CCRT) on these biomarkers and their association with treatment outcome was investigated. Design: This study included ninety-seven cervical cancer patients and thirty controls. Three oxidative stress parameters (8-hydroxy-2-deoxyguanosine, Protein Carbonyl, and Malondialdehyde) and four antioxidant parameters (Superoxide Dismutase, Catalase, Glutathione Peroxidase, and Total Antioxidant Status) were measured. The analysis was conducted using repeated measures ANOVA for comparing among the phases (before, during, and follow-up) of treatment. The control group was compared using the Dunnet test. Logistic regression analysis was also conducted between oxidative stress and antioxidant parameters to study their association. Results: Significant rises in oxidative damage markers were observed in cervical cancer patients of all stages, compared to controls. There was a further increase in oxidative stress markers during CCRT among complete responders. However, among non-responders, the oxidative stress biomarkers like Protein Carbonyl and Malondialdehyde were unaltered during CCRT. Simultaneously, there was a significant decrease in antioxidant parameters in cervical cancer patients of all stages compared to controls. During CCRT, antioxidant levels continuously depleted among complete responders. Nevertheless, in non-responders, antioxidant parameters like Superoxide Dismutase and Total Antioxidant Status were consistent. The oxidative stress markers and antioxidant parameters normalized among complete responders at six months follow up. While in non-responders, the normalization of these parameters was not observed. Conclusion: Our results indicate that increased oxidative stress and diminished antioxidants among patients were associated with carcinoma cervix. Induced oxidative stress and decreased antioxidant parameters during CCRT among the complete responders show the treatment's efficacy. Oxidant-antioxidant profile merits investigation as markers of diagnosis, treatment response, survival, and recurrence in extensive prospective studies. © 2021 Elsevier Inc.
An inexpensive method of small photon field dosimetry with EBT3 radiochromic film
(Springer Verlag, 2018) Ankur Mourya; Lalit Mohan Aggarwal; Abhijit Mandal; Uday Pratap Shahi; Chhape Ram; Anupam Kumar Asthana; Satyajit Pradhan
The relative output factor for small field photon beams with EBT3 (External beam therapy, Gafchromic ™) Radiochromic film were obtained using indigenously developed a program in MATLAB. MLC and JAWS created square small field sizes for dosimetry. To calibrate Radiochromic film, we have cut the film into several small segments of 6 × 6 cm2. These small pieces of film were irradiated to known doses ranging from 25 to 300 cGy by SAD technique using 6 MV photon beam from Linear accelerator. A Program in MATLAB was written to analyze the scanned film data and all the images of the films were imported into this Program. To validate the accuracy of calibration curve, radiochromic films were irradiated with different field sizes of 10 × 10 cm2, 15 × 1 5 cm2, 20 × 20 cm2 to known dose of 200 cGy each by SAD technique. Field sizes were defined using two methods, in the first method fields were defined using X-Y collimator Jaws with the MLC retracted to their maximum position. In the Second method, MLCs were used to define square field sizes of 1 × 1 cm2 to 5 × 5 cm2, 10 × 10 cm2 and 15 × 15 cm2. Calibration curve created using gafchromic film show percentage variation between measured and delivered dose within 3% of standard field size. Output factors measured by Gafchromic EBT3 film showed close agreement with those measured using the other detector for field sizes of 2.0 × 2.0 cm2 and above. EBT3 film has properties such as linear response, energy, and dose-rate independence. It makes it suitable detector for small field dosimetry. Our Indigenous developed MATLAB Program for film dosimetry gave the desired results. From this study, we can conclude that EBT3 film can be used for relative output factors ranging from small field to large fields. © 2018, IUPESM and Springer-Verlag GmbH Germany, part of Springer Nature.
Anti-tumor activity of staurosporine in the tumor microenvironment of cervical cancer: An in vitro study
(Elsevier Inc., 2015) Suresh Singh Yadav; Chandra Bhushan Prasad; Shyam Babu Prasad; Lakshmi Kant Pandey; Sunita Singh; Satyajit Pradhan; Gopeshwar Narayan
Aim The fundamental events for cancer progression and metastases include loss of cell adhesion, cell proliferation, anchorage-independent cell growth (evading anoikis), cell migration and cell invasion. All these events leading to cancer progression happen in a favorable nurturing tumor microenvironment. This study was designed to explore the anti-tumor activity of staurosporine (a nonspecific protein kinase inhibitor) in the tumor microenvironment of cervical cancer. Main methods The anti-tumor activity of staurosporine was investigated by cell adhesion assay, colony formation assay, apoptosis assay and quantitative real-time polymerase chain reaction (PCR) in cervical cancer cell lines. Key findings The cell adhesion assay showed that staurosporine induces adhesion of cervical cancer cells to the extracellular matrix (ECM) protein fibronectin. The soft agar colony formation assay showed that staurosporine inhibits both the number and size of colony formation in a dose dependent manner and also induces adherent tendency in the cancer cells. Staurosporine also induces prominent apoptosis in single cell suspensions compared to adherent cells. Stroma cell induced transcription of matrix metalloprotease 1 (MMP1) and matrix metalloprotease 2 (MMP2) in cervical cancer cells was inhibited by staurosporine. Significance Our results indicate that staurosporine induces anti-tumor response in the cervical tumor microenvironment by inhibiting the fundamental events for cancer progression and metastases. The present study represents an attractive area for further research and opens up new avenues towards the understanding of cervical cancer therapeutics. © 2015 Elsevier Inc. All rights reserved.
Assessment of predictive markers of response to neoadjuvant chemotherapy in breast cancer
(2010) Mallika Tewari; Satyajit Pradhan; Usha Singh; Taj Bali Singh; Hari Shankar Shukla
OBJECTIVE: To identify the predictive markers associated with chemotherapy sensitivity, especially those producing pathological complete response (pCR) following neoadjuvant chemotherapy (NACT) in patients with locally advanced breast cancer. METHODS: Core needle biopsy of 50 locally advanced breast cancer patients was analysed for histopathology, grade, oestrogen receptor, progesterone receptor, HER2, Ki-67, p53, Bcl-2, and BAX before starting NACT. This was correlated with response to NACT using Response Evaluation Criteria in Solid Tumours criteria. RESULTS: The mean tumour reduction rate per chemotherapy cycle was significantly higher in BAXpositive (p = 0.01) and Bcl-2-negative (p = 0.04) tumours. BAX expression significantly (p = 0.043) correlated with a response of an at least 30% reduction in tumour size post-NACT on multivariate analysis. A significant relationship was seen between loss of Bcl-2 expression and pCR on univariate (p = 0.048) analysis. Overall, all of the above 12 parameters had 30.4% and 28.5% success in predicting clinical complete response and pCR, respectively, by the Cox and Snell formula. CONCLUSION: Of all parameters examined, only the apoptosis-related genes (Bcl-2 and BAX) seemed to exert some influence on the response to NACT, and neither by itself was sufficient to predict pCR; however, 50 patients is not sufficient to simultaneously analyse several predictive markers. © 2010 Asian Surgical Association.
Automated medical image segmentation techniques
(2010) Neeraj Sharma; Amit K. Ray; K.K. Shukla; Shiru Sharma; Satyajit Pradhan; Arvind Srivastva; Lalit Aggarwal
Accurate segmentation of medical images is a key step in contouring during radiotherapy planning. Computed topography (CT) and Magnetic resonance (MR) imaging are the most widely used radiographic techniques in diagnosis, clinical studies and treatment planning. This review provides details of automated segmentation methods, specifically discussed in the context of CT and MR images. The motive is to discuss the problems encountered in segmentation of CT and MR images, and the relative merits and limitations of methods currently available for segmentation of medical images.
Automating IMRT planning for cervical cancer using dimension-scaled prior-based Vanilla Bayesian optimization
(2025) Vinay Saini; Sanjay Barman; Satyajit Pradhan; Abhishek Shinghal; Ankita Rungta Kapoor; Neeraj K. Sharma
Objective.Manual inverse planning for radiation therapy is labor-intensive and often prone to inconsistent plan quality due to multiple adjustable planning hyperparameters, varying planner experience, and differing time constraints. Automated treatment planning offers a solution to these challenges. The present study investigates the effectiveness of dimension-scaled prior (DSP) Vanilla Bayesian optimization (BO) with a log-expected improvement (logEI) acquisition function in automating intensity-modulated radiation therapy (IMRT) planning for cancer cervix (CaCx) in high-dimensional settings.Approach.A Python-based auto-optimization script utilizing DSP Vanilla BO with logEI was employed to iteratively optimize the planning hyperparameters, including dose objectives and their corresponding weights for CaCx case IMRT plans on the Varian Eclipse treatment planning system (TPS) v18.0. This approach was assessed in 30 retrospectively selected pelvic node-positive CaCx cases, and the dosimetric parameters, based on EMBRACE-II protocol, were compared with plans generated from manual, Sparse Axis-Aligned Subspace BO (SAASBO), and stopping criterion-based DSP Vanilla BO.Main results.DSP Vanilla BO plans demonstrated superior dose conformity (CI95%PTV45&CI80%PTV45) and organs at risk (OAR) sparing (V40GyBowel,V30GyBowel,V40GyBladder,V40GyRectum,D0.01%femoral heads,Dmeanfemoral heads, andDmeankidneys) compared to manual planning with significant improvements (p< 0.05), while maintaining adequate clinical target coverage for CTVN, PTV55, ITV45, and PTV45. Compared to SAASBO, DSP Vanilla BO achieved comparable dosimetric quality but with less computation time (∼94 vs 360 min). The addition of stopping criteria further reduced the optimization time to ∼44 min while maintaining a plan quality comparable to manual planning.Significance.The study demonstrated that DSP Vanilla BO automated plans achieved comparable target coverage, with an improvement in OAR sparing, compared to manual plans. This highlights its effectiveness as an efficient, data-independent method for automating IMRT planning, which can be easily integrated into a TPS and benefit clinics with limited resources. © 2025 Institute of Physics and Engineering in Medicine. All rights, including for text and data mining, AI training, and similar technologies, are reserved.
Benzothiazole derivative bearing amide moiety induces p53-mediated apoptosis in HPV16 positive cervical cancer cells
(Springer, 2020) Arusha Modi; Meenakshi Singh; Gopichand Gutti; Ozasvi R. Shanker; Vinay Kumar Singh; Sunita Singh; Sushil Kumar Singh; Satyajit Pradhan; Gopeshwar Narayan
In our previous study, we screened the anti-cancer properties of 10 benzothiazole derivatives in cervical cancer cell lines. In the present study, we aimed to delineate the mechanism of the apoptotic pathway (whether intrinsic or extrinsic) following the treatment of N-(4-(benzo[d]thiazol-2-yl)phenyl)-5-chloro-2-methoxybenzamide (named as A-07) on cervical cancer cell lines. Cellular stress by reactive oxygen species was measured using DCFDA dye by flowcytometry. Protein expression and localization was checked by immunofluorescence for γH2A.X, TP53, and CASP-3. Expression profiles of BAX and BCL-2 was done by semi-quantitative RT-PCR and PARP-1 (Poly(ADP-ribose) polymerase-1) by Western blot analysis. Bioinformatic studies were done using PDB websites, metaPocket 2.0 server, YASARA software and Discovery Studio 3.5 Visualizer. We demonstrate that the compound A-07 leads to ROS generation and double strand breaks in SiHa and C-33A cells. The induction of apoptosis in SiHa cells is associated with increased nuclear expression of the tumor suppressor protein, TP53. The shift in BAX/BCL-2 ratio, increased expression of Caspase-3 and cleaved Poly(ADP-ribose) polymerase-1 favour apoptotic signal in SiHa. In silico studies revealed that A-07 has inhibiting capabilities to the E6/E6AP/P53 complex. Our data suggest that treatment of A-07 causes p53 and caspase dependent apoptosis in HPV 16 infected SiHa cells. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Cancer pattern in Varanasi district from Uttar Pradesh state of India, a foundation for cancer control based on the first report of the population-based cancer registry
(Wolters Kluwer Medknow Publications, 2024) Atul M. Budukh; Satyajit Pradhan; Virendra B. Singh; Divya Khanna; Sonali S. Bagal; Priyal S. Chakravarti; Anand N. Sharma; Rajesh K. Vishwakarma; Shraddha S. Shinde; Naveen C. Khargekar; Pankaj Chaturvedi; Rajesh P. Dikshit; Vijay K. Shukla; Rajendra A. Badwe
Background: The cancer registry provides reliable data from the population. In this article, we provide cancer burden and its patterns from the Varanasi district. Methods: The method adopted by the Varanasi cancer registry is community interaction along with regular visits to more than 60 sources to collect data on cancer patients. The cancer registry was established by the Tata Memorial Centre, Mumbai, in 2017 covering 4 million population (57% rural and 43% urban population). Results: The registry has recorded 1,907 incidence cases (1,058 male and 849 female). The age-adjusted incidence rate per 100,000 population in male and female of Varanasi district is 59.2 and 52.1, respectively. One in 15 male and one in 17 female are at risk of developing the disease. Mouth and tongue cancers are the predominant cancers in male, whereas breast, cervix uteri, and gallbladder are the leading cancer sites among the female. In female, cervix uteri cancer is significantly higher (double) in rural areas when compared with urban areas (rate ratio [RR] 0.5, 95% confidence interval [CI; 0.36, 0.72]), whereas in male, mouth cancer is higher in urban areas when compared with rural areas (RR 1.4, 95% CI [1.11, 1.72]). More than 50% of cancer cases in male are due to tobacco consumption. There may be underreporting of the cases. Conclusion: The results of the registry warrant policies and activities related to early detection services for the mouth, cervix uteri, and breast cancers. The Varanasi cancer registry is the foundation for cancer control and will play an important role in the evaluation of the interventions. © 2024 Indian Journal of Cancer.
Carcinoma of the gallbladder presenting as scalp tumour
(W.B. Saunders Ltd, 1998) Manoj Pandey; Nakul C. Aryya; Satyajit Pradhan; A.K. Asfhana; Amitabh Gautam; Vijay K. Shukla
Carcinoma of the gallbladder is characterized by rapid tumour growth associated with lymphatic and local tumour invasion. The peritoneum, GIT and lungs are common sites of seeding. Distant metastasis to bone rarely occurs. Here we document a case of silent gallbladder carcinoma presenting as scalp tumour with improved survival.
Color Doppler ultrasound as an objective assessment tool for chemotherapeutic response in advanced breast cancer
(2005) Seema Singh; Satyajit Pradhan; Ram Chandra Shukla; Mumtaz Ahmad Anson; Anand Kumar
Background: In our part of the world, the majority of the patients with breast cancer present with locally advanced disease and require neo-adjuvant chemotherapy as the primary treatment modality. It is essential to monitor the response to chemotherapy in these patients. Clinical examination as the sole criterion of response assessment is entirely subjective and fallacious. Magnetic Resonance Imaging (MRI) and Computed Tomography (CT) are expensive. The role of Doppler ultrasonography as an imaging modality for this purpose is therefore being evaluated. Methods: A prospective study was undertaken of 25 cases of locally advanced breast carcinoma (LABC) and Color Doppler sonography was used for the sequential assessment of chemotherapeutic response. The response assessed on the basis of clinical examination and Color Doppler was compared with the histological response. The parameters assessed on color Doppler were a change in the number of flow signals, maximum flow velocity (V max), pulsatility index (PI) and resistivity index (RI). Responses were analysed statistically using the Pearson correlation coefficient and Kappa statistics (κ). The sensitivity, specificity, positive predictive & negative predictive values for predicting complete histological response were calculated. Results: Color Doppler showed a sensitivity of 88.88% for predicting complete histological response. The negative predictive value of color Doppler was 92.3%. A significant correlation was obtained between color Doppler & histopathological response. Conclusions: Color Doppler was found to be an objective and effective tool or modality compared with clinical evaluation in sequential response assessment, especially for predicting complete histological response.
CXCL12 is a key regulator in tumor microenvironment of cervical cancer: an in vitro study
(Springer Netherlands, 2016) Suresh Singh Yadav; Shyam Babu Prasad; Chandra Bhushan Prasad; Lakshmi Kant Pandey; Satyajit Pradhan; Sunita Singh; Gopeshwar Narayan
CXCL12 is a small pro-inflammatory chemo-attractant cytokine which signals through chemokine receptor CXCR4. The importance of CXCL12/CXCR4 axis is coming to the fore in several divergent signaling pathway-initiating signals related to cell survival and/or proliferation and cancer metastasis. In the present study we have investigated whether deregulation in CXCR4 signaling (as a consequence of deregulated expression of CXCL12) modulate the metastatic potential of cervical carcinoma cells. We demonstrate that CXCL12 is frequently down regulated and its promoter is hypermethylated in cervical cancer cell lines and primary tumor biopsies. Exogenous treatment of cervical cancer cell lines (HeLa, SiHa and C-33A) with recombinant CXCL12 inhibited the metastasis promoting cell migration, cell invasion and anchorage independent cell growth events. Although this study will need further in vivo validation, our observations suggest that (a) silencing of CXCL12 in cervical cancer cells may be critical in migration and invasion, the key events in cancer cell metastases; (b) cervical cancer cells having down regulated CXCL12 are more prone to being attracted to CXCL12 expressed at secondary sites of metastases; and (c) CXCL12 inhibits anchorage independent cell growth via anoikis. These findings suggest the tumor suppressor functions of CXCL12 in cervical cancer. © 2016, Springer Science+Business Media Dordrecht.
Diagnostic and prognostic application of Raman spectroscopy in carcinoma cervix: A biomolecular approach
(Elsevier B.V., 2021) Anju Shrivastava; Lalit Mohan Aggarwal; Chilakapati Murali Krishna; Satyajit Pradhan; Surendra Pratap Mishra; Sunil Choudhary; Chandan Bhai Patel; Saurav Singla; Ashish; Ranjan Kumar Singh
Blood serum samples from 63 cervical cancer patients and 30 controls were collected at three different phases of the treatment (i.e. before, during, and at follow up). The spectra of serum samples from control as well as patients were classified into different groups using principal component analysis (PCA) and linear discriminant analysis (LDA) based on different phases of treatment using R software. The spectra of blood serum samples have shown the distinct changes and differences compared with each other in the profile of various biochemical parameters. The sensitivity (92.5%) and specificity (85%) were observed maximum between control and cervical cancer patients (before treatment). Between different phases of treatment, the sensitivity and specificity were less but, all accuracies of detection and classification reached above 50%. This method can be considered as a screening method for detection and treatment monitoring. © 2020
Doppler ultrasound scoring to predict chemotherapeutic response in advanced breast cancer
(2007) Anand Kumar; Seema Singh; Satyajit Pradhan; Ram C. Shukla; Mumtaz A. Ansari; Tej B. Singh; Rohit Shyam; Saroj Gupta
Background: Doppler ultrasonography (US) is increasingly being utilized as an imaging modality in breast cancer. It is used to study the vascular characteristics of the tumor. Neoadjuvant chemotherapy is the standard modality of treatment in locally advanced breast cancer. Histological examination remains the gold standard to assess the chemotherapy response. However, based on the color Doppler findings, a new scoring system that could predict histological response following chemotherapy is proposed. Methods: Fifty cases of locally advanced infiltrating duct carcinoma of the breast were studied. The mean age of the patients was 44.5 years. All patients underwent clinical, Doppler and histopathological assessment followed by three cycles of CAF (Cyclophosphamide, Adriamycin and 5-Fluorouracil) chemotherapy, repeat clinical and Doppler examination and surgery. The resected specimens were examined histopathologically and histological response was correlated with Doppler findings. The Doppler characteristics of the tumor were graded as 1-4 for <25%, 25-50%, >50% and complete disappearance of flow signals respectively. A cumulative score was calculated and compared with histopathological response. Results were analyzed using Chi square test, sensitivity, specificity, positive and negative predictive values. Results: The maximum Doppler score according to the proposed scoring system was twelve and minimum three. Higher scores corresponded with a more favorable histopathological response. Twenty four patients had complete response to chemotherapy. Sixteen of these 24 patients (66.7%) had a cumulative Doppler score more than nine. The sensitivity of cumulative score >5 was 91.7% and specificity was 38.5%. The area under the ROC curve of the cumulative score >9 was 0.72. Conclusion: Doppler scoring can be accurately used to objectively predict the response to chemotherapy in patients with locally advanced breast cancer and it correlates well with histopathological response. © 2007 Kumar et al; licensee BioMed Central Ltd.