Browsing by Author "Satyendra Pratap Singh"

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A scientometric analysis on WoS reports to evaluate the research on Fusarium oxysporum since 2009
(Taylor and Francis Ltd., 2019) Prachi Singh; Anukool Vaishnav; Satyendra Pratap Singh; Jagajjit Sahu
Fusarium oxysporum is one of the most devastating fungal pathogen, worldwide having wide host range, infecting large number of monocot and dicot. In the present study, scientometrics approach has been used to summarise all study on F. oxysporum in a single article. We collected a total 5358 reports from Web of Science (WoS) since 2009 to till date and analysed using R scripts. The average citations per documents and annual growth percentage of 10.66 and 1.44, respectively, were obtained. Most productive country was China with 951 followed by India with 774 articles. However, based upon the citation parameters, India became the top most country with 9781 citations and China came on the second place with 9024 citations. When topical classification was performed, Plant science was found to contain maximum of 1522 documents. Collectively, information on F. oxysporum synthesised and identified gaps, which are most deserving for exploration. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Auxins of microbial origin and their use in agriculture
(Springer Science and Business Media Deutschland GmbH, 2020) Chetan Keswani; Satyendra Pratap Singh; Laura Cueto; Carlos García-Estrada; Samia Mezaache-Aichour; Travis R. Glare; Rainer Borriss; Surya Pratap Singh; Miguel Angel Blázquez; Estibaliz Sansinenea
To maintain the world population demand, a sustainable agriculture is needed. Since current global vision is more friendly with the environment, eco-friendly alternatives are desirable. In this sense, plant growth–promoting rhizobacteria could be the choice for the management of soil-borne diseases of crop plants. These rhizobacteria secrete chemical compounds which act as phytohormones. Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class which regulates various processes of plant growth. IAA compound, in which structure can be found a carboxylic acid attached through a methylene group to the C-3 position of an indole ring, is produced both by plants and microorganisms. Plant growth–promoting rhizobacteria and fungi secrete IAA to promote the plant growth. In this review, IAA production and mechanisms of action by bacteria and fungi along with the metabolic pathways evolved in the IAA secretion and commercial prospects are revised. Key points • Many microorganisms produce auxins which help the plant growth promotion. • These auxins improve the plant growth by several mechanisms. • The auxins are produced through different mechanisms. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
Bacillus spp. as Bio-factories for Antifungal Secondary Metabolites: Innovation Beyond Whole Organism Formulations
(Springer, 2023) Bruno Salazar; Aurelio Ortiz; Chetan Keswani; Tatiana Minkina; Saglara Mandzhieva; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Akansha Jain; Harikesh B. Singh; Estibaliz Sansinenea
Several fungi act as parasites for crops causing huge annual crop losses at both pre- and post-harvest stages. For years, chemical fungicides were the solution; however, their wide use has caused environmental contamination and human health problems. For this reason, the use of biofungicides has been in practice as a green solution against fungal phytopathogens. In the context of a more sustainable agriculture, microbial biofungicides have the largest share among the commercial biocontrol products that are available in the market. Precisely, the genus Bacillus has been largely studied for the management of plant pathogenic fungi because they offer a chemically diverse arsenal of antifungal secondary metabolites, which have spawned a heightened industrial engrossment of it as a biopesticide. In this sense, it is indispensable to know the wide arsenal that Bacillus genus has to apply these products for sustainable agriculture. Having this idea in our minds, in this review, secondary metabolites from Bacillus having antifungal activity are chemically and structurally described giving details of their action against several phytopathogens. Knowing the current status of Bacillus secreted antifungals is the base for the goal to apply these in agriculture and it is addressed in depth in the second part of this review. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2025) Aurelio Ortiz; Estibaliz Sansinenea; Keswani Chetan; Tatiana Mikhailovna Minkina; Satyendra Pratap Singh; Bhagwan Narayan Rekadwad; Rainer Borriss; Kathleen Laura Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2024) Aurelio Ortiz; Estibaliz Sansinenea; Chetan Keswani; Tatiana Minkina; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Kathleen Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Consortium of compatible Trichoderma isolates mediated elicitation of immune response in Solanum melongena after challenge with Sclerotium rolfsii
(Taylor and Francis Ltd., 2019) Kartikay Bisen; Shatrupa Ray; Satyendra Pratap Singh
Biocontrol agents contribute to the multifaceted benefits associated with sustainable agriculture. In the current study, Solanum melongena seeds were subjected to single as well as consortial application of two compatible Trichoderma isolates (BHU51 and BHU105) for assessment of growth promotion and defence induction against Sclerotium rolfsii. Minimum damping-off (19.67%) was recorded in Trichoderma consortium treated seeds as compared to the pathogen inoculated control (56.67%). Physiological assays suggested an overall increase in phenolic content, particularly shikimic acid (SA), gallic acid (GA), t-cholorogenic acid (CHA) and syringic acid (SGA) in Trichoderma consortia treated plants upon S. rolfsii infection. In addition, defence-related enzymes and antioxidants also showed significantly higher activities in Trichoderma-treated hosts, especially upon application of the consortium. Thus, the above study provides a comparative evaluation of individual as well as consortial application of Trichoderma isolates on host physiology, an indispensible step towards sustainable agriculture. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Effect of hydrolysed Trifolium repens L. extract on menopause induced obesity and depressive symptoms: an in-vitro and in-silico approach
(Taylor and Francis Ltd., 2025) Poonam Rawat; B. Kumar; Ankita N. Misra; Satyendra Pratap Singh; Surya Pratap Singh; Sharad Kumar Srivastava
This study aimed to evaluate acid-hydrolysis effects on phytoestrogen content and the efficacy of phytoestrogen-rich extract in alleviating post-menopausal symptoms (PMS). Bioactive phytoestrogens were quantified using HPTLC, and unhydrolyzed and hydrolysed extracts were assessed for oestrogenic, anti-proliferative, MAO-A inhibition, ROS quenching, and PL inhibition potential through in-vitro models. In-silico analysis of quantified phytoestrogens against PMS-related proteins was conducted. Hydrolysis increased phytoestrogen content significantly, particularly biochanin-A (56.48-fold), daidzein (22.2-fold), genistein (10.77-fold), and formononetin (5.26-fold). The hydrolysed extract demonstrated improved efficacy in in-vitro models, including reduced IC50 in oestrogenic activity, decreased cell proliferation, increased MAO-A inhibition, and decreased IC50 for lipase. High binding energies of phytoestrogens against ERβ, MAO-A, and PL supported their potential in managing post-menopausal symptoms. The study scientifically validated T. repens for women’s health at menopause, highlighting acid-hydrolysis for enhancing therapeutic activity. T. repens could be explored commercially as an alternative to T. pratense. © 2025 Informa UK Limited, trading as Taylor & Francis Group.
Nano-Inputs: A Next-Generation Solution for Sustainable Crop Production
(Springer, 2023) Satyendra Pratap Singh; Chetan Keswani; Tatiana Minkina; Aurelio Ortiz; Estibaliz Sansinenea
A sustainable agriculture is a challenge for human health. In the agriculture field, the factors like stress created due to the climate change and the newly emerging pathogens, their recognition, and the intensity of damage that they can do are some of the vital challenges in plant disease management worldwide. There is an urgent call for adoption of environmentally friendly biopesticides/biofertilizers to apply to agriculture. In this context, nanomaterials promise to support this transition by promoting mitigation, enhancing productivity, and reducing contamination. Nanotechnology can be used in agriculture to reduce the use of chemicals, decrease nutrient losses, and increase crops’ yield. The nanotechnology can be applied in the form of nanofertilizers and nanopesticides to increase the crops production. Besides, nanosensors act monitoring soil quality of agricultural field and maintaining the health of agricultural plants. Although large-scale production and in-field testing of nano-agrochemicals are still ongoing, the collected information indicates improvements in uptake, use efficiency, targeted delivery of the active ingredients, and reduction of leaching and pollution. In this review we summarize how nanotechnology impacts in agriculture. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Nanomaterial-based biosensors: a new frontier in plant pathogen detection and plant disease management
(Frontiers Media SA, 2025) Jeetu Narware; Jharjhari Chakma; Satyendra Pratap Singh; Divya Raj Prasad; Jhumishree Meher; Prachi Singh; Priya Bhargava; Shraddha Bhaskar Sawant; Pitambara; Jyoti Prakash Singh; Nazia Manzar; Abhijeet Shankar Kashyap
Nanotechnology has significantly advanced the detection of plant diseases by introducing nano-inspired biosensors that offer distinct advantages over traditional diagnostic methods. These biosensors, enhanced with novel nanomaterials, exhibit increased sensitivity, catalytic activity, and faster response times, resulting in improved diagnostic efficiency. The increasing impact of climate-induced stress and emerging plant pathogens have created an urgent demand for real-time monitoring systems in agriculture. Nanobiosensors are revolutionizing plant disease management by enabling on-site detection of pests and weeds, facilitating precise pesticide applications. This article provides a comprehensive overview of the development and application of nanobiosensors in real-time plant disease diagnosis. It highlights key innovations, such as smartphone-integrated nanozyme biosensing and lab-on-a-chip technologies. Special emphasis is placed on the detection of molecular biomarkers, demonstrating the critical role of nanobiosensors in addressing the evolving challenges of plant disease management and agricultural sustainability. © © 2025 Narware, Chakma, Singh, Prasad, Meher, Singh, Bhargava, Sawant, Pitambara, Singh, Manzar and Kashyap.
Paradendryphiella arenariae (MW504999) as a Novel Fungal Source of Tenuazonic Acid in Tomato (Lycopersicon esculentum)
(Springer, 2025) Ankita Kumari; Karuna Singh; Neha Tiwari; Diksha Katiyar; Satyendra Pratap Singh; Anurag Mishra
Tenuazonic acid (TeA) is a mycotoxin usually produced by Alternaria species. Its toxicological potency is considered to be the highest among all Alternaria-mycotoxins. The present study for the first time reports Paradendryphiella arenariae isolated from tomato (Lycopersicon esculentum) as a source of TeA mycotoxin, thus adding a new genus to the array of TeA-producing fungi. The study involves optimizing culture conditions for maximum TeA production, and employing analytical techniques to characterize the compound. Thin-layer chromatography and high-pressure liquid chromatography (HPLC) were employed for the isolation and characterization of the mycotoxin produced by P. arenariae. Structural elucidation was achieved using Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Quantitative determination of TeA was conducted using HPLC with a standard TeA reference. The presence of TeA was further confirmed through electronspray ionization-mass spectrometry and high-resolution liquid chromatography-mass spectrometry. In cytotoxicity assays, the isolated TeA exhibited significant toxicity to murine splenocytes, with an IC50 of 25 µg/mL. This study highlights the need for vigilance regarding TeA contamination in food products. The identification of P. arenariae as a new source of TeA underscores the importance of expanding monitoring efforts to include diverse fungal species. Ensuring food safety through stringent regulations and routine testing is essential to mitigate health risks associated with TeA exposure. © Association of Microbiologists of India 2025.
The Impact of Biofortified Vermicompost on Enhancing Nutritional Quality, Micronutrient Content, and Antioxidants in Carrot (Daucus Carota Cv Surbhi)
(Springer Science and Business Media Deutschland GmbH, 2025) Jharjhari Chakma; Jeetu Narware; Satyendra Pratap Singh; Dawa Dolma Bhutia; Amitava Rakshit
Purpose: Beneficial soil microbes are pivotal players in the intricate dance of nutrient cycling. Their abundance in the soil and its nutrient reservoir hinges on the soil’s structure and functions, influencing nutrient mobilization and uptake. This study explores the effects of biofortified vermicompost on improving the nutritional quality, micronutrient content, and antioxidants in carrots. By analyzing the influence of this formulation on the growth and development of carrots, we aim to shed light on its potential benefits for enhancing the overall health benefits of this popular vegetable. Methods: Carrots were cultivated in pots under nine different treatments, with vermicompost biofortified using various combinations of three beneficial microbes: Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluroscence, along with a control. Results: The results of the study revealed that the combination of T. harzianum and B. subtilis produced the most significant results (p < 0.05) across all parameters tested, including micronutrient content in carrot root (Fe, 77.65 ± 0.65 mg kg− 1) Cu (12.67 ± 0.12 mg kg− 1) and in soil Mn (18.13 ± 0.07 mg kg− 1), Zn (28.43 ± 0.08 mg kg− 1), antioxidant activity, and biochemical analysis. Following closely behind was the combination of B. subtilis and P. fluroscence where the treatment has shown best in micronutrient content of carrot root (Zn, 51.84 ± 2.48 mg kg− 1) and soil (Fe, 20.12 ± 0.03 mg kg− 1and Cu, 8.71 ± 0.40 mg kg− 1). Conclusions: Positive outcomes in all the parameters have been observed when beneficial microorganisms were recruited along with vermicompost in combination with different microorganisms and using the microorganisms individually, suggesting a strong correlation between plant-microbe interactions and improving the overall quality of carrots. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2025.
Trichoderma spp. mediated induction of systemic defense response in brinjal against Sclerotinia sclerotiorum
(Elsevier Ltd, 2021) Satyendra Pratap Singh; Chetan Keswani; Surya Pratap Singh; Estibaliz Sansinenea; Trinh Xuan Hoat
Induction of resistance to pathogen is associated with the colonization of root by Trichoderma spp. has been attributed as one of the major mechanisms contributing to pathogenic invasion. The present study sheds light on the defense network of brinjal plant bioprimed with Trichoderma spp. challenged with Sclerotinia sclerotiorum. Plants treated with dual inoculation of Trichoderma harzianum and Trichoderma asperellum triggered further synthesis of TPC under S. sclerotiorum challenge with maximum increment recorded at 72 hours. In consortium treated and pathogen challenged plants, a higher amount of shikimic acid was observed at 72 hours, whereas other phenolics showed little differences among the treatments. The consortium treatment showed significantly higher defense related enzymes (Phenylalanine Ammonia Lyase, Peroxidase and Polyphenol Oxidase) activity than other treatments. The study signifies how Trichoderma spp. reprograms the host's defense network to provide robust protection against S. sclerotiorum. In the present case, overall protection was provided to the brinjal plants against the attack of S. sclerotiorum. © 2021 The Author(s)
Trichoderma-Mediated Synthesis of ZnONPs: Trend Efficient for Repressing Two Seed- and Soil-Borne Phytopathogens Phomopsis vexans and Colletotrichum capsici
(Springer, 2024) Rahul Kumar; Satyendra Pratap Singh; Fedor Ivanov; Aleksey Yu Maksimov; Elizaveta Latsynnik; Tatiana Minkina; Chetan Keswani
This study provides insight into the synthesis, optimization, and characterization of zinc oxide nanoparticles (ZnONPs) employing a highly promising Trichoderma isolate. The effectiveness of the synthesized ZnONPs was assessed against two plant pathogenic fungi, Phomopsis vexans, which causes Phomopsis blight in brinjal, and Colletotrichum capsici, which causes fruit rot in chili. ZnONP synthesis was optimized through systematic parameter adjustments, including different ratios of Zn to culture filtrate, varying pH ranges, and different time intervals of synthesis. The synthesized nanoparticles were characterized through diverse analytical techniques, including UV‒vis spectroscopy, scanning electron microscopy (SEM) and energy-dispersive analysis of X-rays (EDAX), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and others. The UV–visible spectra exhibited absorption peaks within the range of 240–380 nm, indicating the formation of ZnO nanoparticles. The size of ZnONPs during SEM and TEM analysis was found to be 20–30 nm and 9–25 nm, respectively. These analyses yielded valuable insights into the morphology, size distribution, crystalline structure, and functional groups present in the nanoparticles and their antimicrobial properties. Furthermore, the antifungal efficacy of the synthesized ZnONPs was assessed against P. vexans and C. capsici at various concentrations. The nanoparticles demonstrated strong antifungal activity in vitro at 200–500 ppm concentrations against both pathogens. The outcome of this study highlights the potential of using Trichoderma-derived ZnONPs as a sustainable and effective approach for managing plant fungal diseases. Overall, this research contributes to the expanding field of nanoparticle-based agricultural solutions and underscores the significance of understanding nanoparticle-fungus interactions for practical applications in crop protection. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Unraveling efficient strategies for inducing systemic resistance in crops for managing biotic stress
(Elsevier B.V., 2023) Chetan Keswani; Raffaella Balestrini; Tatiana Minkina; Satyendra Pratap Singh; Ugo De Corato; Estibaliz Sansinenea
[No abstract available]
Unravelling the Dynamic Role of Beneficial Microbes in Regenerative Agriculture
(CRC Press, 2024) Rahul Kumar; Kapil Jindal; J.P. Singh; Satyendra Pratap Singh
The concept of sustainable agriculture is a well-discussed practice ensuring that it ensures the resilient socio-economic status of a farm without compromising its ill effects on our environment. Although Regenerative Agriculture (RA) is a relatively new term with no set meaning, it is a farming concept based on the notion that “no-size-fits-all”, and it works by fixing degraded land through zero tillage, reducing chemical application, and strengthening agriculture by means of nature-based resources. RA is the next step in sustainable agriculture (SA). Due to the increasing human population, there is immense pressure on the farming world to feed them with increased crop production, for which farmers injudiciously use pesticides and synthetic fertilizers without thinking of their side effects. Here comes the role of beneficial microbes in rejuvenating and healing the soil, water, and plant systems. Unraveling the biota black box using modern molecular methods can help us to find modern molecular suits for beneficial organisms. The incorporation of some of these microbes, viz., Rhizobium, Pseudomonas, Cyanobacteria, Trichoderma, Mycorrhizae, etc., can certainly help to boost up agricultural production globally. Crop productivity is greatly influenced by soil management practices, which influence the microbial diversity and soil microbial processes. Microbes function as a community, not in isolation. This chapter focuses on the diverse roles of beneficial microbes and various processes helping in the regeneration of agriculture. © 2024 CRC Press.
Unravelling the multifarious role of wood vinegar made from waste biomass in plant growth promotion, biotic stress tolerance, and sustainable agriculture
(Elsevier B.V., 2025) Sumit Kumar; Mehjebin Rahman; Ali Chenari Bouket; Reza Ahadi; Mukesh Kumar Meena; Ingudam Bhupenchandra; Udai B. Singh; Rameshkumar Arutselvan; Ravindra Kumar; Satyendra Pratap Singh; Abhijeet Shankar Kashyap; Ruchi K. Tripathi; Sachin Gupta; Pranab Dutta; undefined Harish; Ramesh Pratap Singh; Prashant Swapnil
The population of the world has increased intensively, and sustainable agriculture practices are important in achieving the zero-hunger goal. The agriculture sector is constantly facing serious problems caused by climate change and the occurrence of pesticide-resistant causal agents. In this context, one of the most crucial eco-friendly approaches, e.g., using plant-derived bioproducts, is gaining more attraction because they have multifarious potential to overcome challenges. Wood vinegar (WV) or pyroligneous acid (PA) is a liquid biomaterial that is produced by the thermo-pyrolysis process of woody biomass and it was defined as a reddish-brown aqueous liquid that contains methanol, acetic acid, tars, and wood oils by the distillation process of woods. PA production and their application in agricultural fields, have attracted more attention recently because they may work as good bio-stimulants, biopesticides, and biofertilizers. Therefore, it may open a novel, promising window for agriculture and food production. PA has a significant role in elevating plant agronomic activities such as seed germination and vigor index, plant growth, nutritional value, and crop yields. This review aims to discuss in detail the following items: 1) The composition of wood vinegar and its production system; 2) Seed priming with the PA process; 3) Investigation of the role of PA in plant growth promotion activities, biocontrol potential, and its applications for alleviating biotic stresses; 4) Describe the molecular mode of action of PA in suppressing plant diseases causal agents and promoting plant immunity through a well-illustrated diagram; 5) Evaluation of PA role in soil physicochemical, biological, and enzymatic activities and its impact on improving compost quality and curtailing emissions of green house gasses; 6) PA different advantages and limitations, as well as future perspectives for its usage and development. © 2024 Elsevier B.V.