Browsing by Author "Saurabh Pandey"

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Calcium homeostasis and potential roles to combat environmental stresses in plants
(Elsevier B.V., 2022) Rubab Shabbir; Talha Javed; Sadam Hussain; Sunny Ahmar; Misbah Naz; Hina Zafar; Saurabh Pandey; Jyoti Chauhan; Manzer H. Siddiqui; Chen Pinghua
The calcium (Ca+2) molecules being an important intracellular messenger are involved in various signal transduction mechanisms in plants. Many external stimuli such as drought, cold, heat stress, metalloid stress (copper (Cu), cadmium (Cd), nickel (Ni), and arsenic), flooding, and salinity stress increase the free Ca+2 ions in the cytosol. The H+/Ca+2 antiporters and Ca+2-ATPases actively transport the cytosolic Ca+2 in intracellular organelles or apoplasts. Increase in Ca+2 concentration is sensed by calcium-binding proteins or Ca+2-sensors which lead to activation of CDPKs (Calcium-dependent protein kinases). These CDPKs regulate various genes responsive to stress to show phenotypic responses against stress stimuli. Hormonal signaling and their crosstalk with Ca+2 have been studied extensively but a thorough understanding of Ca+2 in stress tolerance is limited. This review describes the various aspects of Ca+2 involvement in sensing stress stimuli, signal transduction, role against various stress factors (including temperature extreme, salinity, flooding, metalloids, and drought), the role of Ca+2 in the regulation of physiological processes, and Ca+2-ATPase. © 2022 SAAB
Crucial Cell Signaling Compounds Crosstalk and Integrative Multi-Omics Techniques for Salinity Stress Tolerance in Plants
(Frontiers Media S.A., 2021) Rajesh K. Singhal; Debanjana Saha; Milan Skalicky; Udit N. Mishra; Jyoti Chauhan; Laxmi P. Behera; Devidutta Lenka; Subhash Chand; Vivek Kumar; Prajjal Dey; Indu; Saurabh Pandey; Pavla Vachova; Aayushi Gupta; Marian Brestic; Ayman El Sabagh
In the era of rapid climate change, abiotic stresses are the primary cause for yield gap in major agricultural crops. Among them, salinity is considered a calamitous stress due to its global distribution and consequences. Salinity affects plant processes and growth by imposing osmotic stress and destroys ionic and redox signaling. It also affects phytohormone homeostasis, which leads to oxidative stress and eventually imbalances metabolic activity. In this situation, signaling compound crosstalk such as gasotransmitters [nitric oxide (NO), hydrogen sulfide (H2S), hydrogen peroxide (H2O2), calcium (Ca), reactive oxygen species (ROS)] and plant growth regulators (auxin, ethylene, abscisic acid, and salicylic acid) have a decisive role in regulating plant stress signaling and administer unfavorable circumstances including salinity stress. Moreover, recent significant progress in omics techniques (transcriptomics, genomics, proteomics, and metabolomics) have helped to reinforce the deep understanding of molecular insight in multiple stress tolerance. Currently, there is very little information on gasotransmitters and plant growth regulator crosstalk and inadequacy of information regarding the integration of multi-omics technology during salinity stress. Therefore, there is an urgent need to understand the crucial cell signaling crosstalk mechanisms and integrative multi-omics techniques to provide a more direct approach for salinity stress tolerance. To address the above-mentioned words, this review covers the common mechanisms of signaling compounds and role of different signaling crosstalk under salinity stress tolerance. Thereafter, we mention the integration of different omics technology and compile recent information with respect to salinity stress tolerance. © Copyright © 2021 Singhal, Saha, Skalicky, Mishra, Chauhan, Behera, Lenka, Chand, Kumar, Dey, Indu, Pandey, Vachova, Gupta, Brestic and El Sabagh.
Drought stress responses and inducing tolerance by seed priming approach in plants
(Elsevier B.V., 2022) Debanjana Saha; Prince Choyal; Udit Nandan Mishra; Prajjal Dey; Bandana Bose; Prathibha MD; Narendra Kumar Gupta; Brijesh Kumar Mehta; Pawan Kumar; Saurabh Pandey; Jyoti Chauhan; Rajesh Kumar Singhal
Field crops are subjected to drought at different growth stages and cause for substantial yield loss in major crops, thus threaten to global food security. The crop researcher have evaluated numerous physiological, biochemical and molecular strategies to combat drought stresses but these approaches are not enough in present scenario. Therefore, it is argued that plants can be primed by assorted organic and in-organic promoters for excelling fortitude under stress conditions. Hence, seed priming with different agents is an auspicious area of research in stress biology and crop stress management, for conferring tolerance when plants are subjected to drought stress. However, the adaptation and tolerance mechanisms of drought stress are complex and quantitative in nature, which have been explored at physiological, biochemical and molecular levels thoroughly in this review. The concept of stress memory and its implication in future generation has also been discussed. Finally, in this review the challenges and opportunities of seed priming with effective application in crop stress management along with expanding the knowledge on deep understanding of drought stress tolerance to reduce the future yield gap are discussed thoroughly. © 2022
Editorial: Emerging pollutants in the environment: impact and challenges for agriculture and nutritional security
(Frontiers Media SA, 2023) Rajesh Kumar Singhal; Saurabh Pandey; Sudhakar Srivastava
[No abstract available]
Evaluation of Uterocervical Angle and Cervical Length as Predictors of Spontaneous Preterm Birth
(Georg Thieme Verlag, 2022) Pramod Kumar Singh; Resham Srivastava; Ishan Kumar; Sangeeta Rai; Saurabh Pandey; Ram C. Shukla; Ashish Verma
Aim The aim of this article was to evaluate uterocervical angle (UCA) and cervical length (CL) measured at 16 to 24 weeks of gestation using transvaginal sonography (TVS) as predictors of spontaneous preterm birth. Methods In this prospective study, TVS was performed in 159 primigravidas with a singleton, uncomplicated pregnancy at 16 to 24 weeks of gestation to measure the anterior UCA and CL. All the cases were followed until labor to document gestational age at delivery. Results The risk of spontaneous preterm birth was higher in women with obtuse UCA (>95 degrees) with sensitivity of 86.7%, specificity of 93.0%, positive predictive value of 83.0%, negative predictive value of 94.6%, and p -value of <0.001. The difference between the means was statistically significant (p -value < 0.001). UCAs ≥105degrees and 95 to 105 degrees were found to be significantly associated with spontaneous preterm births at <34 weeks and 34 to 37 weeks, respectively. CL <2.5 cm was found to predict spontaneous preterm births at <37 weeks with sensitivity of 31.1%, specificity of 95.6%, and p -value of <0.001. UCA was found to be a better predictor of spontaneous preterm birth with a higher coefficient of variation (56.4%) when compared with CL (16.9%). Conclusions UCA proved to be a novel ultrasound parameter that can serve as a better predictor of spontaneous preterm births in comparison to CL. A strong correlation exists between obtuse UCA and a risk of spontaneous preterm birth. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Mycobacterium tuberculosis adaptation to host environment
(Elsevier, 2022) Aniruddha Banerjee; Shatabdi Sengupta; Nishant Nandanwar; Monika Pandey; Deeksha Tripathi; Saurabh Pandey; Ashutosh Kumar; Vidyullatha Peddireddy
Mycobacterium tuberculosis, a pathogen that causes tuberculosis encounters a variety of stresses and extreme conditions such as acidic conditions, hypoxic and immune system stress, metal, and heat shocks during host infection. It withstands these hostile environments by employing various survival strategies. The lung macrophages are the primary immune cells that interact with M. tuberculosis after infection. Various proteins of M. tuberculosis are responsible for the survival of M. tuberculosis in acidic and hypoxic conditions inside the host. M. tuberculosis tolerates temperature variations with the help of various heat shock proteins (Hsp) such as Hsp70, Hsp22.5, HspR, and the protein Acr2, which is an active member of α-crystalline family of molecular chaperones. M. tuberculosis also overcomes toxic concentrations of various metal ions. M. tuberculosis fulfills iron requirements by the acquisition of iron using siderophores such as mycobactins and carboxymycobactins. © 2023 Elsevier Inc. All rights reserved.
Plant photosynthesis under abiotic stresses: Damages, adaptive, and signaling mechanisms
(Elsevier B.V., 2023) Jyoti Chauhan; M.D. Prathibha; Prabha Singh; Prince Choyal; Udit Nandan Mishra; Debanjana Saha; Rajeev Kumar; Hirdayesh Anuragi; Saurabh Pandey; Bandana Bose; Brijesh Mehta; Prajjal Dey; K.K. Dwivedi; N.K. Gupta; Rajesh Kumar Singhal
Photosynthesis is crucial for sustaining life on this planet and necessary for plant growth and development. Abiotic stresses such as high and low temperatures, and excess, or deficit of water limit the crucial plant processes, thus threatening the global food security. However, recent molecular approaches allowed elucidation of the photosynthetic components/compounds and their efficiency under stress conditions. In the present scenario, these approaches are not enough to reduce the yield penalty due to the reduction in photosynthetic efficiency. Therefore, comprehensive data on plant behavior and stress crosstalk networks could assist in understanding the in-depth mechanism of photosynthesis. In recent years, information regarding crosstalk, signaling characterization of candidate genes, and responses to multiple stressors have advanced our knowledge to understand the mechanism of photosynthesis. Therefore, in this review, we provide a comprehensive overview of various studies conducted on photosynthesis under multiple abiotic stress factors that affect the photosynthetic efficiency of a plant. We also discuss the role of crosstalk signaling compounds (plant growth regulators and micro RNAs) for an in-depth understanding of the photosynthesis mechanism. Finally, based on our gathered data set, the mechanism of damage and adaptive response of photosynthesis under multiple stressors are explained to enhance the scientific community's knowledge toward boosting photosynthesis and to accelerate stress tolerance strategies for crop improvement. © 2023
Plant stress phenotyping: Current status and future prospects
(Academic Press Inc., 2024) Vishal Dinkar; Sayantan Sarkar; Saurabh Pandey; Suresh H. Antre; Amarjeet Kumar; R. Thribhuvan; Ashutosh Singh; Ashish Kumar Singh; Badal Singh; Md. Afjal Ahmad
Scientists aim to improve crop response under stress conditions and gain better yields in continuously changing environmental conditions. They rely on plant phenotyping to quantify crop response under adverse conditions to achieve this goal and select the most tolerant genotypes. Recent advances in phenotyping platforms allow dissecting of complex traits such as abiotic stress. For example, the phenotyping platform is integrated with artificial intelligence (AI) and remote sensing tools to provide more robust, high throughput data collections in real-time changing environments. This review will give a deep understanding of the requirement of phenomics in crop improvement under stress conditions. We have discussed different phenotyping platforms, suitable traits for phenotyping, and machine learning and AI integration with the high throughput phenotypic platform for collecting a large data set of crops under stress conditions. Overall our review will dissect the phenomics aspects of complex traits, such as biotic and abiotic stress-related traits requiring sensor advancement, high-quality imagery combined with machine learning methods, and efforts in transdisciplinary science to foster integration across disciplines and better our understanding of plant stress biology. © 2024 Elsevier Inc.
Pyrene-Appended Luminescent Probes for Selective Detection of Toxic Heavy Metals and Live Cell Applications
(John Wiley and Sons Inc, 2024) Ankur Srivastava; Gargi Mishra; Amit Kumar Pathak; Saurabh Pandey; Chhama Awasthi; Mrituanjay D. Pandey; Kamalakanta Behera
Heavy metal contamination has become a global environmental problem and currently drawn much attention from researchers in worldwide. High exposure to heavy metals can lead to problems like kidney, liver and brain damage, skin and lung cancer, etc. Rapid and sensitive detection of heavy metals is of immense importance in environmental monitoring. Over the past few decades, enormous efforts have been made to design various sensors to identify and monitor these harmful metal ions. In Comparison with other methods, fluorescence-based chemosensors have received extensive attention because of the advantages of high sensitivity, low cost, real-time monitoring, simple operation, etc. The pyrene derivatives are significant polycyclic aromatic hydrocarbons (PAHs) with strong fluorescence. They are widely used as fluorescent probes in many applications due to their easy modification, high fluorescence quantum yield, and strong fluorescence emission in live cells, outstanding cell permeability, and very low cytotoxicity. The SDG 6: Clean water and sanitation alarm a strong strategy, and careful identification of the risk connected to environmental pollutants needs urgent attention in this situation. Therefore, we highlight different types of pyrene-based fluorophores and the fluorescence mechanisms for the sensitive and selective detection methodology of Arsenic, Mercury, Cadmium, Lead, and Chromium heavy metals. © 2024 Wiley-VCH GmbH.
Roles of Nitric Oxide in Conferring Multiple Abiotic Stress Tolerance in Plants and Crosstalk with Other Plant Growth Regulators
(Springer, 2021) Rajesh Kumar Singhal; Hanuman Singh Jatav; Tariq Aftab; Saurabh Pandey; Udit Nandan Mishra; Jyoti Chauhan; Subhash Chand; Indu; Debanjana Saha; Basant Kumar Dadarwal; Kailash Chandra; Mudasser Ahmed Khan; Vishnu D. Rajput; Tatiana Minkina; Eetela Sathya Narayana; Manoj Kumar Sharma; Shahid Ahmed
Nitric oxide (NO) is a free-radical gasotransmitter signaling molecule associated with a varied spectrum of signal transduction pathways linked to inducing cross-adaptation against abiotic stresses. It has crucial roles from seed germination to plant maturity, depending upon its cellular concentration. The functional cross-talk of NO among different stress signaling cascades leads to alteration in the expression of developmental genes that regulate biosynthesis and function of plant growth regulators (PGRs). NO-PGRs and secondary signaling compounds cross-talk trigger reprogramming of stress-responsive gene expressions, transcriptional gene modulations, redox regulating machinery, oxidative metabolisms, and multiple regulatory pathways under plant abiotic stress. Recent findings suggest NO as critical components of numerous plant signaling network that interplays with auxin, gibberellins (GA), abscisic acid (ABA), ethylene (ET), jasmonic acid (JA), brassinosteroids (BRs), H2O2, melatonin, hydrogen sulfide (H2S), salicylic acid (SA), and other PGRs to modulate growth and development under multiple stresses. Considering the importance of NO signaling crosstalk under stress adaptation, in this review, we point out the biosynthesis and metabolism of NO and its crosstalk with numerous other signaling compounds. Further, recent cellular and molecular advances in NO signaling cross-talk under abiotic stress adaptations also have been discussed. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Seed priming with Mg(NO3)2 and ZnSO4 salts triggers physio-biochemical and antioxidant defense to induce water stress adaptation in wheat (Triticum aestivum L.)
(Elsevier B.V., 2021) Rajesh Kumar Singhal; Saurabh Pandey; Bandana Bose
Wheat is a widely grown cereal crop that provides >20% daily protein intake and calories worldwide. Drought stress drastically affects the crucial physiological (relative water content; RWC), biochemical (proline content), and molecular attributes in wheat plants from germination to maturity stage and ultimately reduces the yield and quality traits. Given this, the present work was conducted to know the effect of salt seed priming on wheat crop. For this, we have used two wheat variety HUW-468 (V1), and HUW-510 (V2) primed with water (T2), Mg(NO3)2 (T3), ZnSO4 (T4), and combinations of Mg(NO3)2 and ZnSO4 salt (T5) with non-primed seed (T1) under water stress (W1) and control (W0) conditions. We have observed significant differences in plant height (84 to 90 cm), leaf number, total leaf area (431.82 to 505.46 cm2 plant−1), relative water content, chlorophyll content (4.78 to 5.86 mg g−1fresh weight), membrane stability index (MSI) (56.25 to 61.42%), superoxide dismutase (SOD) activity (133.47 to 150.10), MDA content, proline content (101.64 to 100.46), nitrate reductase activity (NRA), and nutrient (N, Zn, and Fe) contents under control and stress conditions. These results concluded that seed priming with a combination of Mg(NO3)2 and ZnSO4 salt is best for improved wheat crop traits under drought stress compared to individual salt and non-priming treatments. Overall, this analysis elucidated that seed priming strengthens the antioxidant capacity under drought conditions by enhancing antioxidant enzymes and alleviating oxidative stress. © 2021 The Author(s)
Structural and functional prediction of WsMYB34 transcription factor in Withania somnifera (L.) Dunal by deciphering its role in NaCl-induced secondary metabolism
(Elsevier B.V., 2023) Lakee Sharma; Bipin Maurya; Saurabh Pandey; Krishna Kumar Rai; Shashi Pandey-Rai
In the present study, a MYB transcription factor from Withania somnifera, was identified i.e., WsMYB34, known for development and stress tolerance in plants. MYB transcription factors have been widely implicated in conferring stress tolerance in medicinal plants. To validate this notion, we analyzed the impact of different concentrations of NaCl on W. somnifera and hypothesized the potentiality of MYB protein in regulating the expression of secondary metabolite-related genes by docking approach. Since structural and functional characteristics of R2R3 type MYB TF in W. somnifera remain largely unknown, in-silico analysis was exploited to understand conserved binding motifs, phylogeny, 3-D structure, and role in combating stress. Based on these outcomes, WsMYB34 was found to be an R2R3 type MYB protein, which is phylogenetically close to that of Capsicum baccatum (CbMYB34). In addition, in-silico interaction analysis depicted the binding capacity of WsMYB34 protein on the promoter region of a triterpenoid synthesising gene viz CYP85A69 (having CAACTG cis-regulatory sequence). The comparative microarray analysis also offered the role of MYB34 in abiotic stress tolerance in model plants. Exposure to NaCl imposed increased withanolide and flavonoid contents and significant up-regulation of key genes for withanolide and WsMYB34 gene by 0.93–3.68 and 2.61–3.47-folds respectively. The enhanced expressivity of transcript encoding WsMYB34 indicated its possible role in inducing terpenes, flavonoids, anthocyanins, and trichome organogenesis to provide defence against salt. In addition, NaCl caused a reduction in morphology and photosynthesis of W. somnifera. However, enhanced contents of phenolics, proline, MDA, and free radical scavenging activities were observed. © 2023 Elsevier B.V.