Browsing by Author "Shashank Kumar Mishra"

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Comprehensive illustration of transcriptomic and proteomic dataset for mitigation of arsenic toxicity in rice (Oryza sativa L.) by microbial consortium
(Elsevier Inc., 2022) Surabhi Awasthi; Reshu Chauhan; Yuvraj Indoliya; Abhishek Singh Chauhan; Shashank Kumar Mishra; Lalit Agrawal; Sanjay Dwivedi; Shiv Naresh Singh; Suchi Srivastava; Poonam C. Singh; Puneet Singh Chauhan; Debasis Chakrabarty; Sudhakar Srivastava; Rudra Deo Tripathi
The present article represents the data for analysis of microbial consortium (P.putida+C.vulgaris) mediated amelioration of arsenic toxicity in rice plant. In the current study the transcriptome profiling of treated rice root and shoot was performed by illumina sequencing (Platform 2000). To process the reads and to analyse differential gene expression, Fastxtoolkit, NGSQCtoolkit, Bowtie 2 (version 2.1.0), Tophat program (version 2.0.8), Cufflinks and Cuffdiff programs were used. For Proteome profiling, total soluble proteins in shoot of rice plant among different treatments were extracted and separated by 2D poly acrylamide gel electrophoresis (PAGE) and then proteins were identified with the help of MALDI-TOF/TOF. In gel based method of protein identification, the isoelectric focusing machine (IPGphor system,Bio-Rad USA), gel unit (SDS-PAGE) and MALDI-TOF/TOF (4800 proteomic analyzer Applied Biosystem, USA) were used for successful separation and positive identification of proteins. To check the differential abundance of proteins among different treatments, PDQuest software was used for data analysis. For protein identification, Mascot search engine (http://www.matrixscience.com) using NCBIprot/SwissProt databases of rice was used. The analyzed data inferred comprehensive picture of key genes and their respective proteins involved in microbial consortium mediated improved plant growth and amelioration of As induced phyto-toxicity in rice. For the more comprehensive information of data, the related full-length article entitled “Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling” may be accessed. © 2022
Metalliferous Soil Remediation Through Heavy Metal-Resistant Plant Growth-Promoting Bacteria: Prospects and Paradigms
(Springer Nature, 2023) Basudev Majhi; Pradeep Semwal; Shashank Kumar Mishra; Vaibhav Srivastava; Rajeev Pratap Singh; Puneet Singh Chauhan
Heavy metals (HMs) are natural assets on the planet, but they have become a serious threat to environmental pollution due to fast industrial enterprise, metropolitanism, and recent technological breakthroughs. The principal causes of HMs pollution in soil, water, and air are industrial waste, organic compounds, pesticides, paints (including small-medium industries), and mining activities. The land, water, and air utilized in farming are of major significance and may have an impact on the health of living organisms. Novel and robust ecotechnologies are needed to avoid HM contamination in the environment. Microbial bioremediation has long been recognized as the most well-understood biotechnological process for environmental restoration. For the treatment of HM-contaminated environ-mental sites, microbial bioremediation is a cost-effective option. Researchers worldwide are making strides in discovering new bacterial strains with plasmid-linked degradation/reduction ability. Genetic engineering and molecular biology aided in the development of microbes that would produce the desired results in the environment. Recent advances in microbial bioremediation techniques include biostimulation, bioaugmentation, bioaccumulation, biosorption, and the use of biofilms. This chapter assembles data on recent developments and applications of microbe-mediated bioremediation of HM-contaminated soils. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2023.