Browsing by Author "Shilpi Chaudhary"

Now showing 1 - 4 of 4

Biogenic synthesis of dual-emission chlorophyll-rich carbon quantum dots for detection of toxic heavy metal ions - Hg(ii) and As(iii) in water and mouse fibroblast cell line NIH-3T3
(Royal Society of Chemistry, 2024) Ravi Pratap; Nurul Hassan; Monika Yadav; Saurabh Kumar Srivastava; Shilpi Chaudhary; Anita Kamra Verma; Jayeeta Lahiri; Avanish S. Parmar
Due to the excessive residues and serious adverse effects, it is urgent to develop an efficient method to detect Hg2+ and As3+ in drinking water to protect human health. In this study, a novel fluorometric sensor based on chlorophyll-rich carbon quantum dots (CQDs) for the detection of Hg2+ and As3+ ions was developed. The dual emitting (blue-green and red) CQDs were synthesized by a one-step solvothermal method using plumeria plant leaves as precursors. When Hg2+ ions were added in the presence of CQDs, the red emission (676 nm) of the CQDs was quenched (OFF), while in the presence of As3+ ions, the blue-green emission (485 nm) was enhanced (ON). The detection limits of 0.99 nM for Hg2+ and 12.15 nM for As3+ were validated for the proposed sensor. The biocompatibility, cytotoxicity, and bioimaging of the CQDs were investigated in the mouse fibroblast cell line NIH-3T3. The cellular uptake was also studied under the influence of Hg2+ and As3+ ions. Moreover, the novel chlorophyll-rich CQD-based fluorometric “ON-OFF” dual probe sensor was successfully applied to detect Hg2+ and As3+ in real water systems as well as in the mouse fibroblast cell line. © 2024 The Royal Society of Chemistry.
Biogenic synthesis of gold nanoparticles using dual extract of tulsi–Vinca for breast cancer tumor regression in mice
(Newlands Press Ltd, 2023) Ravi Pratap; Karishma Niveria; Saurabh Kumar Srivastava; Shilpi Chaudhary; Poonam Sharma; Anita K. Verma; Avanish Singh Parmar
Aim: This work aims to synthesize the gold nanoparticles (GNPs) using a dual extract of tulsi and Vinca (T+V-Gold) for breast cancer tumor regression. Methods: The GNPs were synthesized and characterized for their microscopic, spectroscopic and crystalline properties. Further, the GNPs were investigated for in vitro and in vivo studies for the treatment of the 4T1-induced triple-negative breast cancer murine model. Results: The GNPs for 4T1 tumor-challenged mice resulted in delayed tumor development and lower tumor burden, with T+V-Gold demonstrating the highest prevention of tumor spread. The antitumor effect of T+V-Gold is highly significant in the glutathione family antioxidants glutathione S-transferase and glutathione in tumor tissue samples. Conclusion: The bioefficacy and anticancer outcomes of T+V-Gold nanoformulation can be used as therapeutic agents and drug-delivery vehicles. © 2023 Newlands Press Ltd. All rights reserved.
Effect of carbon quantum dots derived from extracts of UV-B-exposed Eclipta alba on alcohol-induced liver cirrhosis in Golden Hamster
(Springer Nature, 2023) Kshama Rai; Kanchan Yadav; Megha Das; Shilpi Chaudhary; Kaustubh Naik; Priya Singh; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Shashi Bhushan Agrawal; Avanish Singh Parmar
The Eclipta alba plant is considered hepatoprotective, owing to its phytoconstituents wedelolactone. In the current study, effect of elevated ultraviolet-B (eUV-B) radiation was investigated on biochemical, phytochemical, and antioxidative enzymatic activities of E. alba (Bhringraj) plant. The UV-B exposure resulted in an increase in oxidative stress, which has caused an imbalance in phytochemical, biochemical constituents, and induced antioxidative enzymatic activities. It was observed that the UV-B exposure promoted wedelolactone yield by 23.64%. Further, the leaf extract of UV-B-exposed plants was used for the synthesis of carbon quantum dots (CQDs) using low cost, one-step hydrothermal technique and its biocompatibility was studied using in vitro MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay on HepG2 liver cell line. It revealed no toxicity in any treatment groups in comparison to the control. Both CQDs and leaf extract were orally administered to the golden hamster suffering from alcohol-induced liver cirrhosis. In the morphometric study, it was clearly observed that a combination of UV-B-exposed leaf extract and synthesized CQDs delivered the best result with maximum recovery of liver tissues. The present study reveals the positive impact of UV-B exposure on the medicinally important plant, increased yield of wedelolactone, and its enhanced hepatoprotective efficacy for the treatment of damaged liver tissues. Graphical Abstract: [Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
Skin Adhesive 3D-Printable BSA-Amyloid/Cellulose Hybrid Hydrogel Film for Rapid Wound Healing and Skin Regeneration with Enhanced Antioxidant and Anti-Inflammatory Properties
(American Chemical Society, 2025) Saurabh Kumar Srivastava; Shikha Tripathi; Sakshi Agarwal; Rahul Ranjan; Somesh Agrawal; Prodyut Dhar; Eugene B. Postnikov; Shilpi Chaudhary; Vinod N. Tiwari; Avanish Singh Parmar
Natural polymer-based hydrogels closely mimic the extracellular matrix, making them ideal for supporting cell growth and tissue regeneration. Recent advancements in tuning their porosity, morphology, and size have helped overcome key challenges in tissue engineering, such as vascularization and multicellular integration. However, their clinical use is often limited by drawbacks, such as low mechanical strength, structural instability, high production costs, and limited reproducibility. In this work, we present a skin-adhesive, 3D-printable/injectable hybrid hydrogel composed of natural protein and cellulose. This hybrid hydrogel overcomes the limitations of conventional systems by enhancing mechanical strength, scaffold stability, reproducibility, cost-effectiveness, and adhesive properties while preserving high biocompatibility and biodegradability. Using the same formulation, a wound dressing material is fabricated and applied at the wound site either by suturing or as an adhesive film. Furthermore, the hydrogel exhibits inherent antibacterial, antioxidant (60% of radical scavenging), anti-inflammatory, cell viability (up to 90%), and cell migration properties that significantly promote wound healing. This multifunctional hybrid hydrogel offers a promising solution for next-generation wound dressing applications and contributes to the advancement of bioactive and customizable materials in regenerative medicine. © 2025 American Chemical Society