Browsing by Author "Soni Tiwari"

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Multistep Structural and Chemical Evaluation of Sugarcane Baggase, Pretreated With Alkali for Enhancing the Enzymatic Saccharification by Cellulase and Xylanase of the Pseudomonas sp. CVB-10 (MK443365) and Bacillus paramycoides T4 (MN370035) Mix-Culture System
(Frontiers Media S.A., 2022) Soni Tiwari; Janardan Yadav; Rajeeva Gaur; Ranjan Singh; Tuhina Verma; Jay Shanker Yadav; Prabhash Kumar Pandey; Srikanta Kumar Rath
The enzymatic saccharification of sugarcane bagasse was significantly increased by alkali pretreatment under mild conditions. The effectiveness of different concentrations of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of lignocellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% celluloses, 25.63% hemicelluloses, and 30.36% lignin. After that, sugarcane bagasse was pretreated with 5 and 10% of H2SO4 and NaOH at 121°C for 60 min. FTIR, XRD, and SEM analyses also showed significant molecular and surface structure changes of the sugarcane bagasse with 10% NaOH. Maximum saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75, 301.25, and 276.6 mg/g from 10% H2SO4, 5% NaOH, and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1, and 20.6 times higher than the that of the control. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 (MK443365) and Bacillus paramycoides T4 (MN370035) were used to hydrolyze the pretreated bagasse, and the optimal condition was determined to be 30 h of the enzymatic reaction with the 3:1 ratio of enzymes under the temperature of 55°C, pH 5.0, and substrate concentration of 3%, leading to celluloses and hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient. Copyright © 2022 Tiwari, Yadav, Gaur, Singh, Verma, Yadav, Pandey and Rath.
Salt Tolerant Indigenous Zn Solubilizing Bacteria Isolated from Forest Organic Soils Promotes Yield and Root Growth in Oryza Sativa under Zinc Deficient Alluvial Soil
(Taylor and Francis Ltd., 2022) Jaya Prajapati; Janardan Yadav; Durgesh Kumar Jaiswal; Brajkishor Prajapati; Soni Tiwari; Jayshankar Yadav
The present study conducted to identifying and characterizing the efficient Zn solubilizing rhizobacteria isolated from chickpea and peas rhizosphere owing distinct plant growth advancing qualities for zinc-inadequate alluvial soil. Out of ten, Four strains ZnJ11, (Klebsiella pneumonia- MT509804), ZnJ10 (Acinetobacter calcoaceticus- MT509803), ZnJ15A (Acinetobacter pittii- MT509805), and ZnJ2A (Pantoea agglomerans- MT509806) were selected using basal medium contained insoluble Zn minerals such as ZnO and ZnPO4 at concentrations of 2000 µg/mL. In qualitative assay, ZnJ11 showed maximum zinc solubilization efficiency (SE: 525 ± 2.9 and pH-7.5), followed by ZnJ10 showed (SE: 383 ± 3.1 and 329 ± 3.2, pH-7.5) in all three Zn sources, then ZnJ15A and ZnJ2A exhibited (SE: 306 ± 2.2 and 206 ± 1.7) respectively. Contrary to plate assay, zinc solubilization in liquid medium quantified by AAS also revealed efficacy of all the strains. Bacterial growth and Zn solubilization at variable salt concentration (0–10%) and pH (4.5–10) was measured that showed Klebsiella sp. tolerate (8% & 6.5–8.5), Acinetobacter calcoaceticus (6% & 6.5–10), Acinetobacter pittii (10% & 6.5–10) and Pantoea sp. (8% & 5.5–10) respectively. In addition, all strains were showed different degree of susceptibility to tested antibiotics at variable concentrations. Simultaneously, phytostimulating properties like IAA production, nitrogen fixation, phosphate solubilization, potassium solubilization, siderophore production, ammonia production, HCN and amylase, cellulose, pectinase and catalase were also tested. Bio-inoculation of A. calcoaceticus × P. agglomerans was done in rice crop (HUR 105) with different combination of zinc oxide and zinc sulfate in pot experiment. Findings revealed that root growth (31 ± 1.6), straw yield (32.4 ± 1.7), and grain yield (13 ± 2.0) in the treatment of ZnO (Zn-8.0 kg/ha) with Bio-inoculation was found superior over all treatments. The positive action of Bio-inoculation was also exhibited on growth and yield parameters. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
Three-Step Purification and Characterization of Organic Solvent-Tolerant and Alkali-Thermo-Tolerant Xylanase from Bacillus paramycoides T4 [MN370035]
(MDPI, 2022) Soni Tiwari; Ranjan Singh; Janardan Yadav; Rajeeva Gaur; Anurag Singh; Jay Shankar Yadav; Prabhash Kumar Pandey; Santosh Kumar Yadav; Jaya Prajapati; Pukhrambam Helena; Jayant Dewangan; Farrukh Jamal
In the present study, an extracellular alkali-thermo-tolerant xylanase from Bacillus paramycoides was produced in the presence of an organic solvent. The enzyme was purified by ammonium sulphate precipitation, gel filtration, and ion exchange chromatography, with an overall recovery of 25.9%. The purified enzyme hada 70 kDa molecular weight (MW) confirmed by SDS-PAGE gel analysis. The maximum enzyme activity was reported at 55 °C and pH 7.0. Xylanase activity and stability were improved in the presence of 30% (v/v) n-dodecane, iso-octane, n-decane, and cyclohexane (7 days). The enzyme activity was improved by Co2+, EDTA, and Triton-X-100 while vigorously repressed by Hg2+ and Cu2+. The purified enzyme showed 1.473 mg/mL Km and 654.017 µg/mL/min Vmax values. The distinctive assets of the isolate verified the potential application in the field of biomass conversion into fuel and other industrial processes. Organic solvent-tolerant xylanases can be used for concurrent saccharification and bioethanol production, the amplification of intoxicating beverages, and the fermenting industry. © 2022 by the authors.