Browsing by Author "Soumya Ranjan Mohanty"

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Circadian desynchronization in pregnancy of Golden hamster following long time light exposure: Involvement of Akt/FoxO1 pathway
(Elsevier B.V., 2022) Megha Das; Soumya Ranjan Mohanty; Tarun Minocha; Nitesh Kumar Mishra; Sanjeev Kumar Yadav; Chandana Haldar
Coordination between central and peripheral reproductive clocks in females is poorly understood. Long light is having a hazardous effect on reproductive health. Hence, explored the effect of long-time light exposure (LLD; 16L:8D) on the central and peripheral reproductive (ovary and uterus) clock genes (Bmal1, Clock, Per1, Per2, Cry1 and Cry2) and its downstream regulators (Aanat, Egf, Cx26, Cx43, ERα, pAktS-473, pAktT-308, pFoxO1T-24, 14–3-3, HoxA10, HoxA11 and Pibf) expression in non-pregnant and pregnant Golden hamster. Young adult Golden hamsters were exposed to LLD for 30 days and then were mated. We observed that LLD exposure increased the thickness of the endometrium and reduced myometrium thickness, resembling uterine adenomyosis. In non-pregnant females LLD altered the expressions of clock genes in suprachiasmatic nuclei (SCN), ovary and the uterus along with serum estradiol rhythm. LLD upregulated Egf and downregulated Aanat, Cx26, and Cx43 mRNA levels in uterus. LLD upregulated Akt/FoxO1 phosphorylation and 14-3-3 expressions in the uterus of nonpregnant females. LLD exposure to pregnant females lowered serum progesterone, Aanat, Pibf, Hoxa10, and Hoxa11 mRNA expressions on D4 (peri-implantation) and D8 (post-implantation) resulting in a low implantation rate on D8 (post-implantation). Hence it is evident that the frequent pregnancy anomalies noted under a long light schedule might be due to desynchronization in Aanat, Pibf, Hoxa10, and Hoxa11 as well as the central and peripheral clock genes (Bmal1, Clock, Per1, Per2, Cry1 and Cry2). LLD exposure desynchronized the central and peripheral reproductive clock affecting uterine physiology via Akt/FoxO1 pathway in Golden hamsters. Thus, LLD is a risk factor for female reproductive health and fertility. © 2022 Elsevier B.V.
Detecting instant of multiple faults on the transmission line and its types using time-frequency analysis
(Institution of Engineering and Technology, 2019) Shweta Na; Nand Kishor; Kjetil Uhlen; Soumya Ranjan Mohanty
In a complex interconnected network, due to dynamic interaction between the AC networks, during occurrences of faults, the successful relay operation is threatened. For security and reliability of the power system network, a fast and accurate protection scheme is of great importance. This study presents a protection scheme for multiple fault detection at bus/line and its type in a wide area network. If a fault occurs on a line, followed by another fault at the same/different line, before the clearance of former fault, the proposed scheme is capable of detecting such multiple fault events. The scheme applies processed signal information for its time-frequency representation using Smoothed Pseudo Wigner-Ville distribution, followed by Hilbert transform and calculation of indices to interpret the fault events. It is shown that faulted bus, faulted line, time instant, and types of faults can be easily identified with low computational burden. The scheme is validated on the signals simulated on Kundur's model, IEEE 39 bus system and verified with signals on real-time digital simulator for different fault conditions; fault resistance and fault location on the line. The scheme can be successfully applied on signals available from phasor measurement units for wide area network protection. © The Institution of Engineering and Technology 2019.
Impact of STATCOM and BFCL on the performance of distance relay and its improvement by adaptive setting for gridinteractive offshore wind and marine current farm
(Institution of Engineering and Technology, 2020) Satendra Kumar Singh Kushwaha; Soumya Ranjan Mohanty; Paulson Samuel
This study explores the impact of the dynamic impedance of static synchronous compensator (STATCOM) and bridge type fault current limiter (BFCL) on the performance of distance relay and its improvement by adaptive setting for Grid Interactive Offshore Wind and Marine-Current Farm. For assessments, analytical results are presented under different STATCOM settings, low voltage ride through (LVRT) condition, and different types of fault conditions. Moreover, the presence of STATCOM and BFCL significantly changes the apparent impedance characteristic of the studied system. It is also seen that the presence of STATCOM likely to induce an under reach and overreach problem of distance relay. Further, the LVRT capability is significantly affected as STATCOM supplies reactive power to offshore wind generators during a short circuit on the grid side. It is found that the grid integration of OWF and MCF with STATCOM and BFCL is likely to induce mis-coordination and subsequently deteriorate the performance of distance relay, leading to system security problems. Therefore, this study proposed an adaptive setting in order to eliminate the simultaneous influence upon the dynamic impedance due to incorporation of STATCOM and BFCL on the distance relay setting. The simulation results show the efficacy of the proposed adaptive setting. © 2020 The Institution of Engineering and Technology.
Melatonin: A journey from bovine pineal gland to a promising oncostatic agent
(Elsevier Science Ltd., 2020) Tarun Minocha; Megha Das; Nitesh Kumar Mishra; Soumya Ranjan Mohanty; Sanjeev Kumar Yadav
Now a day's cancer is one of the second leading health issue across the globe. Low- and middle-income countries are more prone to cancer and approximately 70% of demises occur in these countries. Melatonin, being a natural signaling molecule is ubiquitously found in almost all the living creatures from organisms to plants. During the past few decades, several studies have inflamed the attention of melatonin as a potent candidate to combat myriad of cancers because of its anti-cancer attribute. Melatonin exhibits the oncostatic property hence, halts the growth of different tumors during different stages of their progression. In a nutshell, this chapter epitomes the therapeutic potential of melatonin against cancer and serves as a concrete platform for researchers to pillar the basic and clinical studies on melatonin for future research directions. © 2021 Elsevier Inc. All rights reserved.
Synthesis, characterization, and anticancer potency of coumarin-derived thiosemicarbazones and their Copper(II) complexes
(Elsevier B.V., 2024) Ramina Maharjan Shrestha; Kuldeep Mahiya; Asmita Shrestha; Soumya Ranjan Mohanty; Sanjeev Kumar Yadav; Paras Nath Yadav
A series of 3-acetylcoumarin-based thiosemicarbazones and their copper(II) complexes were synthesized and characterized by elemental analysis, IR, UV–Vis, HR-ESI Mass, NMR, EPR and single crystal XRD. The XRD study revealed thione tautomer for TSCs 3 (AcCmDEtTSC) and 4 (AcCmDPrTSC) whereas Cu(II) complex 8 [Cu(AcCmDPrTSC)Cl] has distorted square planar geometry around the metal ion. Compound 1 (AcCmMeHTSC) among the thiosemicarbazones (TSCs) was more effective against both breast cancer cell lines: MCF-7 (IC50; 13.02 µg/mL) and MDA-MB-231 (IC50; 42.71 µg/mL), whereas compounds 7 [Cu(AcCmDEtTSC)Cl] (IC50; 32.84 µg/mL) and 8 [Cu(AcCmDPrTSC)Cl] (IC50: 34.69 µg/mL) among the Cu(II) complexes were more effective against MDA-MB-231 cell lines. Anticancer activity of the test compounds enhanced in dose dose-dependent manner against both MCF-7 and MDA-MB-231 cells. Molecular docking study showed significant binding affinity of the test compounds, notably compound 2 (AcCmEtHTSC) with −7.1 kcal/mol and −8.0 kcal/mol, compound 3 with −7.3 kcal/mol and −7.8 kcal/mol against target proteins EGFR and HER2 respectively. Although the IC50 value of the synthesized compounds is not very encouraging, their significant binding affinity values (−5.8 kcal/mol to −8.0 kcal/mol) against the target proteins EGFR and HER 2 is encouraging for the further exploration of their anticancer activity in the future. © 2024 Elsevier B.V.
Synthesis, characterization, anticancer, pharmacokinetics and molecular docking investigation of N (3)-alkyl incorporated-3-acetyl-4-hydroxycoumarin thiosemicarbazones and their copper(II) complexes
(Elsevier B.V., 2024) Ramina Maharjan Shrestha; Kuldeep Mahiya; Asmita Shrestha; Soumya Ranjan Mohanty; Sanjeev Kumar Yadav; Paras Nath Yadav
Coumarin based thiosemicarbazones (TSC), (E)-2-(1-(4‑hydroxy-2-oxo-2H-chromen-3-yl)ethylidene)-N-methylhydrazine-1-carbothioamide (HACmMeTsc) (3), (E)-N-ethyl-2-(1-(4‑hydroxy-2-oxo-2H-chromen-3-yl)ethylidene)hydrazine-1-carbothioamide (HACmEtTsc) (4) and (E)-N,N‑diethyl-2-(1-(4‑hydroxy-2-oxo-2H-chromen-3-yl)ethylidene)hydrazine-1-carbothioamide (HACmDEtTsc) (5) and their respective copper(II) complexes ([Cu(ACmMeTsc)Cl] (3a), [Cu(ACmEtTsc)Cl] (4a) and [Cu(ACmDEtTsc)Cl] (5a)) are synthesized and characterized by elemental analysis, FTIR, UV–Vis, ESI HRMS, 1H NMR, 13C NMR and single crystal X-ray diffraction analysis. The in vitro anticancer activity of the synthesized compounds by MTT assay against breast cancer cell lines MCF-7 and MDA-MB-231 exhibited strong anti-cancer potential in a dose dependent manner with IC50 value in the range of 12.94 -23.70 μg/mL and 42.18- >100 μg/mL in MCF-7 and MDA-MB-231 cells respectively. Molecular docking study showed that compounds 3, 4 and 5 have significant binding affinity (ΔG = -6.8 to -8.4 kcal/mol) along with interactions including hydrogen bonding with active sites of amino acid residues of both studied proteins, EGFR and HER 2, which may be responsible for the inactiveness of the receptors. © 2023 Elsevier B.V.
Tinospora cordifolia Silver Nanoparticles Attenuated the Lipopolysaccharide-induced Testicular Inflammation in Golden Hamster
(Springer Science and Business Media Deutschland GmbH, 2025) Nitesh Kumar Mishra; Kanchan Yadav; Soumya Ranjan Mohanty; Avanish Singh Parmar; Sanjeev Kumar Yadav; Chandana Haldar
Purpose: Testicular inflammation is one of the primary causes of male infertility. Plant-rich remedies are known to reduce testicular inflammation, but the exact mechanism is not known. Tinospora cordifolia (T. cordifolia), an Indian ayurvedic medicinal plant, possesses excellent immunomodulatory properties. We therefore studied the anti-inflammatory potential(s) of green synthesized silver nanoparticles (AgNPs) from T. cordifolia (TC-AgNPs) on the testes of golden hamster. Methods: Aqueous extract of T. cordifolia was used to synthesize the silver nanoparticles (TC-AgNPs), validated by ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry (UHPLC-HRMS). Physical characterization was done using UV–Vis spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared analysis (FTIR) spectroscopy. Biological parameters were measured using enzyme-linked immunosorbent assay (ELISA), western blot analysis, histopathology, photoacoustic, and ultrasound imaging. Results: The UV–Vis surface plasmon resonance (SPR) band at 420 nm confirmed the formation of TC-AgNPs. TEM revealed the semi-spherical morphology of silver nanoparticles with an average diameter of 15 nm. FTIR spectroscopy revealed the presence of multiple functional groups, indicating the bioformulation of silver nanoparticles with T. cordifolia extract. Biological studies with lipopolysaccharide (LPS) challenged male golden hamsters and demonstrated that TC-AgNPs treatment reduced the serum pro-inflammatory cytokine levels (IL-1β, IL-6, and TNF α). The testicular photoacoustic and ultrasound imaging indicated a significant improvement in testicular volume and oxygenation level. Western blot analysis suggests that TC-AgNPs reduced inflammation by downregulating NF-κB protein expression. Furthermore, TC-AgNPs alleviated LPS-induced testicular damages along with an increase in serum testosterone level. Conclusion: The synthesized TC-AgNPs have shown strong anti-inflammatory effects against LPS-induced testicular inflammation in male golden hamsters. Lay Summary: Medicinal plants are increasingly used to treat diseases, with inflammation being a common cause of male infertility. Phytoconstituents are safer and more effective anti-inflammatory agents than synthetic ones. Developing plant-based nanoformulations with high therapeutic efficacy and fewer side effects is essential. T. cordifolia is known for its strong immunomodulatory effects and was used to synthesize TC-AgNPs. Our study found that TC-AgNPs reduced pro-inflammatory cytokines and protected against LPS-induced testicular inflammation in golden hamsters. Further, TC-AgNPs improved testicular volume and oxygen saturation, demonstrating potent anti-inflammatory effects. Future Work/Prospective: Future research should focus on how individual bioactive compounds lower inflammation and explain the molecular mechanisms behind it. © The Author(s), under exclusive licence to The Regenerative Engineering Society 2024.
Tinospora cordifolia Silver Nanoparticles Attenuated the Lipopolysaccharide-induced Testicular Inflammation in Golden Hamster
(Springer Science and Business Media Deutschland GmbH, 2024) Nitesh Kumar Mishra; Kanchan Yadav; Soumya Ranjan Mohanty; Avanish Singh Parmar; Sanjeev Kumar Yadav; Chandana Haldar
Purpose: Testicular inflammation is one of the primary causes of male infertility. Plant-rich remedies are known to reduce testicular inflammation, but the exact mechanism is not known. Tinospora cordifolia (T. cordifolia), an Indian ayurvedic medicinal plant, possesses excellent immunomodulatory properties. We therefore studied the anti-inflammatory potential(s) of green synthesized silver nanoparticles (AgNPs) from T. cordifolia (TC-AgNPs) on the testes of golden hamster. Methods: Aqueous extract of T. cordifolia was used to synthesize the silver nanoparticles (TC-AgNPs), validated by ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry (UHPLC-HRMS). Physical characterization was done using UV–Vis spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared analysis (FTIR) spectroscopy. Biological parameters were measured using enzyme-linked immunosorbent assay (ELISA), western blot analysis, histopathology, photoacoustic, and ultrasound imaging. Results: The UV–Vis surface plasmon resonance (SPR) band at 420 nm confirmed the formation of TC-AgNPs. TEM revealed the semi-spherical morphology of silver nanoparticles with an average diameter of 15 nm. FTIR spectroscopy revealed the presence of multiple functional groups, indicating the bioformulation of silver nanoparticles with T. cordifolia extract. Biological studies with lipopolysaccharide (LPS) challenged male golden hamsters and demonstrated that TC-AgNPs treatment reduced the serum pro-inflammatory cytokine levels (IL-1β, IL-6, and TNF α). The testicular photoacoustic and ultrasound imaging indicated a significant improvement in testicular volume and oxygenation level. Western blot analysis suggests that TC-AgNPs reduced inflammation by downregulating NF-κB protein expression. Furthermore, TC-AgNPs alleviated LPS-induced testicular damages along with an increase in serum testosterone level. Conclusion: The synthesized TC-AgNPs have shown strong anti-inflammatory effects against LPS-induced testicular inflammation in male golden hamsters. Lay Summary: Medicinal plants are increasingly used to treat diseases, with inflammation being a common cause of male infertility. Phytoconstituents are safer and more effective anti-inflammatory agents than synthetic ones. Developing plant-based nanoformulations with high therapeutic efficacy and fewer side effects is essential. T. cordifolia is known for its strong immunomodulatory effects and was used to synthesize TC-AgNPs. Our study found that TC-AgNPs reduced pro-inflammatory cytokines and protected against LPS-induced testicular inflammation in golden hamsters. Further, TC-AgNPs improved testicular volume and oxygen saturation, demonstrating potent anti-inflammatory effects. Future Work/Prospective: Future research should focus on how individual bioactive compounds lower inflammation and explain the molecular mechanisms behind it. © The Author(s), under exclusive licence to The Regenerative Engineering Society 2024.