Browsing by Author "Srishti Ramsaha"

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Fermented papaya preparation modulates the progression of N-methyl-N-nitrosourea induced hepatocellular carcinoma in Balb/c mice
(Elsevier Inc., 2016) Jhoti Somanah; Srishti Ramsaha; Shalini Verma; Ashok Kumar; Poornima Sharma; Ranjan Kumar Singh; Okezie I. Aruoma; Emmanuel Bourdon; Theeshan Bahorun
Aim and main method The medicinal properties of fermented papaya preparation (FPP) derived from Carica papaya fruit was investigated in order to determine its ability to modulate the progression of N-methyl-N-nitrosourea induced hepatocellular carcinoma in Balb/c mice. Key findings As well as reducing the physical symptoms associated with N-methyl-N-nitrosourea (MNU)-induced hepatocellular carcinoma, supplementation of Balb/c mice with 500 mg FPP/kg BW for 92 days normalized the blood cell count, led to an increased activity of several key antioxidant enzymes (SOD: + 20%, CAT: + 81%, GPx: + 66.1%, GR: + 54.4%; P < 0.001 vs. MNU control), increased the ferrous reducing antioxidant potential (+ 36.7%, P < 0.001 vs. MNU control) and reduced the extent of lipid peroxidation in the liver by 44.3% (P < 0.001 vs. MNU control). Significance Results demonstrated the ability of FPP to preserve the integrity of liver against oxidative damage and protect hepatocytes against irreversible DNA structural modifications induced by MNU, highlighting its potential role as an immune-defense modulator during hepatocarcinoma. © 2016 Elsevier Inc.
Modulation of hepatocarcinogenesis in N-methyl-N-nitrosourea treated Balb/c mice by mushroom extracts
(Royal Society of Chemistry, 2016) Srishti Ramsaha; Vidushi S. Neergheen-Bhujun; Shalini Verma; Ashok Kumar; Rahul Kumar Bharty; Amit Kumar Chaudhary; Poornima Sharma; Ranjan Kumar Singh; Priya Huzar Futty Beejan; Kang Kyung-Sun; Theeshan Bahorun
The hepatoprotective potential of edible mushrooms from Mauritius, namely Pleurotus sajor-caju and Agaricus bisporus was evaluated using an N-methyl-N-nitrosourea (MNU)-induced hepatocarcinogenesis Balb/c mice model. Mushroom extracts restored normal weight in MNU treated mice over a 3 month supplementation period. Blood parameter analyses indicated a clear modulation of hemoglobin concentration, leukocyte, platelet, lymphocyte, neutrophil, monocyte and eosinophil counts in MNU-induced mice (p < 0.05). Mushroom extract supplementation effectively reduced oxidative damage in MNU-primed mice, which was marked by a significant decrease in the extent of lipid peroxidation (p < 0.05) and a concomitant increase in the enzymatic antioxidant levels, primarily catalase, superoxide dismutase, glutathione reductase and peroxidase, and FRAP values (p < 0.05). DNA protective effects of the extracts were confirmed by Raman spectroscopy, where, the MNU-DNA interaction, as evidenced by an intense peak at 1254 cm-1, was normalized. The findings demonstrate hepatoprotective, immunomodulatory and anti-carcinogenic effects and suggest the use of mushrooms as potential dietary prophylactics in cancer chemoprevention. © The Royal Society of Chemistry 2016.