Browsing by Author "Sudarshan Maurya"

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Antibiotic potential of plant growth promoting rhizobacteria (PGPR) against Sclerotium rolfsii
(2012) Amitabh Singh; Sudarshan Maurya; Rashmi Singh; U.P. Singh
High performance liquid chromatographic (HPLC) analysis of culture filtrates of plant growth promoting rhizobacteria (PGPR) and medium of inhibitory zone of interaction of Sclerotium rolfsii with PGPR, viz. Pseudomonas aeruginosa, Pseudomonas fluorescens 4, Pseudomonas fluorescens 4 (new) and Pseudomonas sp. varied from sample to sample. In all the culture filtrates of PGPRs, P. aeruginosa had nine phenolic acids in which ferulic acid (14.52 μg/ml) was maximum followed by other phenolic acids. However, the culture filtrates of P. fluorescens 4 had six phenolic acids with maximum ferulic acid (20.54 μg/ml) followed by indole acetic acid (IAA), caffeic, salicylic, o-coumeric acid and cinnamic acids. However, P. fluorescens 4 culture filtrate had seven phenolic acids in which salicylic acid was maximum (18.03 μg) followed by IAA, caffeic, vanillic, ferulic, o-coumeric and cinnamic acids. Pseudomonas sp. also showed eight phenolic acids where caffeic acid (2.75 μg) was maximum followed by trace amounts of ferulic, salicylic, IAA, vanillic, cinnamic, o-coumeric and tannic acids. The analysis of antibiosis zone of PGPRs showed fairly rich phenolic acids. A total of nine phenolic acids were detected in which caffeic acid was maximum (29.14 μg/g) followed by gallic (17.64 μg/g) and vanillic (3.52 μg/g) acids but others were in traces. In P. aeruginosa, antibiosis zone had seven phenolic acids where IAA was maximum (3.48 μg/g) followed by o-coumeric acid (2.08 μg/g), others were in traces. The medium of antibiosis zone of P. fluorescens 4 and P. fluorescens 4 new had eight phenolic acids in which IAA was maximum with other phenolic acids in traces. © 2012 Copyright Taylor and Francis Group, LLC.
Antifungal activity of two alkaloids of Zephyranthes citrina and their field efficacy against powdery mildew (Erysiphe cichoracearum) of balsam
(2010) Udai P. Singh; Leena Gohain; Sudarshan Maurya; Amitabh Singh; Vidya B. Pandey; Ashok K. Singh
The anti-fungal activity of two alkaloids isolated from bulbs of Zephyranthes citrina was observed against 10 fungi, viz, Aternaria solani, A. triticina, Curvularia lunata, C. maculuns, Cercospora malvacearum, Erysiphe sp., Fusarium udum, Helminthosporium pisi, H. speciferum and Ustilago cynodontis. Different concentrations (200, 400, 600, 800, 1000 μg/ml of alkaloids (A and B) were used. Spore germination was inhibited at 600, 800, 1000 μg/ml. B alkaloid was used against Erysiphe cichoracearum causing powdery mildew in balsam (Impatiens balsamina) in the field as pre-and post-inoculation treatments at 1000, 1500, 2000 μg/ml doses. The extract was effective in both pre-and post-inoculation treatments. Foliar application of this alkaloid resulted in inducing synthesis of phenolic acid in the leaves of balsam. Maximum phenolics were detected in the leaves treated with 1500 μg/ml in both pre-and post-inoculation treatments. The increase in the production of phenolics in treated leaves of balsam can be correlated with the induction of resistance in treated plants against powdery mildew. The significant efficacy of the alkaloid under field conditions opens the possibility of its use by farmers for also controlling other diseases. © 2010 Taylor & Francis.
Antimicrobial efficacy, disease inhibition and phenolic acid-inducing potential of chloroform fraction of cow urine
(2012) Udai Pratap Singh; Sudarshan Maurya; Amitabh Singh; Gopal Nath; Mandavi Singh
High-performance liquid chromatographic (HPLC) analysis of chloroform fraction (CF) of cow urine showed rich pool of phenolic acids. Antifungal and antibacterial bioassays of CF have shown its tremendous efficacy against some fungal plant pathogens as well as human pathogenic bacteria at very low concentrations. The CF also inhibited powdery mildew (Erysiphe cichoracearum) of balsam (Impatiens balsamania) under field conditions during pre- and post-inoculation treatments. HPLC analysis of pre- and post-inoculation-treated plant leaves indicated that CF induced phenolic acid synthesis as compared to control. The results revealed that CF of cow urine has the potential for controlling some important human diseases. The result on balsam powdery mildew is a good signal that CF may also be effective against other plant diseases in the field. © 2012 Copyright Taylor and Francis Group, LLC.
Metabolic reprogramming of tomato plants under Ralstonia solanacearum infection
(Elsevier B.V., 2025) Dhananjaya Pratap Singh; Raman Ramesh; Sudarshan Maurya; Suresh Reddy Yerasu; R. Gangaraj; Lovkush Satnami; Ratna Prabha; Renu; Birinchi Kumar Sarma; Nagendra Pal Rai
Comprehensive metabolomic investigation of tomato (Solanum lycopersicum) cultivar Hawaii 7998 and variety Kashi Adarsh was performed to establish metabolic basis of resistance and susceptibility against bacterial wilt pathogen Ralstonia solanacearum. Using LC-MS/MS-based untargeted metabolomics, leaf samples were analyzed at 5 and 10-day post-inoculation, revealing significant metabolic distinctions between the plants. The resistant cultivar Hawaii 7998 demonstrated remarkably lower disease incidence (15.19%) compared to the susceptible variety (86.81%) underpinned by distinct metabolic profiles. Our analysis annotated metabolites across different treatment groups, with significant differential regulation in pathways related to phenylpropanoids, flavonoids, and primary metabolism. Hawaii 7998 exhibited higher constitutive levels of defense-related compounds and mounted more robust metabolic responses against the pathogen. The resistant cultivar Hawaii 7998 under non-treated condition showed enhanced accumulation of total phenolic content (32.81 and 35.17 mg GAE g-1 at 5 and 10DAI respectively) compared to susceptible plants. High antioxidant activities in terms of DPPH (43.52 and 47.19% in non-inoculated and 56.74 and 66.75% in pathogen inoculated condition at 5 and 10DAI respectively) and ABTS (44.36 and 48.06% in control and 58.24 and 64.05% in treated plants) were observed in Hawaii 7998, which was significantly high as compared to Kashi Adarsh. Network analysis showed complex interactions between metabolic pathways, highlighting key regulatory nodes in disease resistance, including carotenoid biosynthesis, trehalose metabolism, and phenylpropanoid pathways. Annotation of biomarker metabolites that included solasodine, biotin, uridine, phosphatidylcholine, asparagine, coumaryl alcohol and linolenic acid revealed cultivar-specific and pathogen interaction specific biomarkers in tomato. These findings are particularly significant in the uncovering the molecular mechanisms of plant-pathogen interaction and offer crucial insights for developing bacterial wilt-resistant tomato varieties, thereby contributing to food security. © 2025
Metabolomics of early blight (Alternaria solani) susceptible tomato (Solanum lycopersicum) unfolds key biomarker metabolites and involved metabolic pathways
(Nature Research, 2023) Dhananjaya Pratap Singh; Sudarshan Maurya; Suresh Reddy Yerasu; Mansi Singh Bisen; Mohamed A. Farag; Ratna Prabha; Renu Shukla; Krishna Kumar Chaturvedi; Md. Samir Farooqi; Sudhir Srivastava; Anil Rai; Birinchi Kumar Sarma; Nagendra Rai; Tusar Kanti Behera
Tomato (Solanum lycopersicum) is among the most important commercial horticultural crops worldwide. The crop quality and production is largely hampered due to the fungal pathogen Alternaria solani causing necrotrophic foliage early blight disease. Crop plants usually respond to the biotic challenges with altered metabolic composition and physiological perturbations. We have deciphered altered metabolite composition, modulated metabolic pathways and identified metabolite biomarkers in A. solani-challenged susceptible tomato variety Kashi Aman using Liquid Chromatography-Mass Spectrometry (LC–MS) based metabolomics. Alteration in the metabolite feature composition of pathogen-challenged (m/z 9405) and non-challenged (m/z 9667) plant leaves including 8487 infection-exclusive and 8742 non-infection exclusive features was observed. Functional annotation revealed putatively annotated metabolites and pathway mapping indicated their enrichment in metabolic pathways, biosynthesis of secondary metabolites, ubiquinone and terpenoid-quinones, brassinosteroids, steroids, terpenoids, phenylpropanoids, carotenoids, oxy/sphingolipids and metabolism of biotin and porphyrin. PCA, multivariate PLS-DA and OPLS-DA analysis showed sample discrimination. Significantly up regulated 481 and down regulated 548 metabolite features were identified based on the fold change (threshold ≥ 2.0). OPLS-DA model based on variable importance in projection (VIP scores) and FC threshold (> 2.0) revealed 41 up regulated discriminant metabolite features annotated as sphingosine, fecosterol, melatonin, serotonin, glucose 6-phosphate, zeatin, dihydrozeatin and zeatin-β-d-glucoside. Similarly, 23 down regulated discriminant metabolites included histidinol, 4-aminobutyraldehyde, propanoate, tyramine and linalool. Melatonin and serotonin in the leaves were the two indoleamines being reported for the first time in tomato in response to the early blight pathogen. Receiver operating characteristic (ROC)-based biomarker analysis identified apigenin-7-glucoside, uridine, adenosyl-homocysteine, cGMP, tyrosine, pantothenic acid, riboflavin (as up regulated) and adenosine, homocyctine and azmaline (as down regulated) biomarkers. These results could aid in the development of metabolite-quantitative trait loci (mQTL). Furthermore, stress-induced biosynthetic pathways may be the potential targets for modifications through breeding programs or genetic engineering for improving crop performance in the fields. © 2023, The Author(s).
Metabolomics Unveiled Metabolic Reprogramming in Tomato Due to Beneficial (Bacillus subtilis) and Pathogenic (Alternaria solani) Tripartite Interaction
(Springer, 2025) Dhananjaya Pratap Singh; Sudarshan Maurya; Suresh Reddy Yerasu; Lovkush Satnami; Nagendra Pal Rai; Ratna Prabha; Renu; Birinchi Kumar Sarma; Tusar Kanti Behera
The interaction between beneficial microbes and pathogens in crop plants can lead to complex metabolic reprogramming. Our study employed LC–MS/MS-based untargeted metabolomics approach to elucidate the metabolic changes in tomato plants induced due to the inoculation of plant growth-promoting rhizobacterium Bacillus subtilis (BV4) and the pathogen Alternaria solani. Multivariate analyses (MVA) revealed distinct metabolic signatures associated with BV4 inoculation, pathogen infection, and their combined treatment. We observed that plant’s inoculation with beneficial microbe BV4 induced up-regulation of metabolites involved in constitutive metabolism, like glycolysis, TCA cycle, amino acid metabolism, and lipid metabolism, potentially supporting plant growth. Pathogen infection majorly triggered up-regulation of specialized metabolites, such as phenylpropanoids, flavonoids, terpenoids, and oxylipins, suggesting enhanced defense responses. The combined treatment however, exhibited a synergistic effect, with up-regulation of metabolites involved in both constitutive and specialized metabolism, suggesting a primed state for defense responses. Galactose metabolism emerged as the most enriched pathway across all treatments indicating its importance in plant defense through cell wall reinforcement, signaling and antimicrobial specialized metabolite production. ROC-based biomarker analysis putatively identified metabolites, including quercetin, salvigenin, delfinidin-3-O-glucoside, and asparagine, as potential biomarkers for distinguishing various treatment conditions. This study provides insights into the metabolic reprogramming in tomato plants in response to beneficial microbe-pathogen interactions and highlights the potential of untargeted metabolomics in elucidating complex plant-microbe interactions. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Metabolomics Unveiled Metabolic Reprogramming in Tomato Due to Beneficial (Bacillus subtilis) and Pathogenic (Alternaria solani) Tripartite Interaction
(Springer, 2024) Dhananjaya Pratap Singh; Sudarshan Maurya; Suresh Reddy Yerasu; Lovkush Satnami; Nagendra Rai; Ratna Prabha; Renu; Birinchi Kumar Sarma; Tusar Kanti Behera
The interaction between beneficial microbes and pathogens in crop plants can lead to complex metabolic reprogramming. Our study employed LC–MS/MS-based untargeted metabolomics approach to elucidate the metabolic changes in tomato plants induced due to the inoculation of plant growth-promoting rhizobacterium Bacillus subtilis (BV4) and the pathogen Alternaria solani. Multivariate analyses (MVA) revealed distinct metabolic signatures associated with BV4 inoculation, pathogen infection, and their combined treatment. We observed that plant’s inoculation with beneficial microbe BV4 induced up-regulation of metabolites involved in constitutive metabolism, like glycolysis, TCA cycle, amino acid metabolism, and lipid metabolism, potentially supporting plant growth. Pathogen infection majorly triggered up-regulation of specialized metabolites, such as phenylpropanoids, flavonoids, terpenoids, and oxylipins, suggesting enhanced defense responses. The combined treatment however, exhibited a synergistic effect, with up-regulation of metabolites involved in both constitutive and specialized metabolism, suggesting a primed state for defense responses. Galactose metabolism emerged as the most enriched pathway across all treatments indicating its importance in plant defense through cell wall reinforcement, signaling and antimicrobial specialized metabolite production. ROC-based biomarker analysis putatively identified metabolites, including quercetin, salvigenin, delfinidin-3-O-glucoside, and asparagine, as potential biomarkers for distinguishing various treatment conditions. This study provides insights into the metabolic reprogramming in tomato plants in response to beneficial microbe-pathogen interactions and highlights the potential of untargeted metabolomics in elucidating complex plant-microbe interactions. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Metabolomics-Driven Mining of Metabolite Resources: Applications and Prospects for Improving Vegetable Crops
(MDPI, 2022) Dhananjaya Pratap Singh; Mansi Singh Bisen; Renu Shukla; Ratna Prabha; Sudarshan Maurya; Yesaru S. Reddy; Prabhakar Mohan Singh; Nagendra Rai; Tribhuwan Chaubey; Krishna Kumar Chaturvedi; Sudhir Srivastava; Mohammad Samir Farooqi; Vijai Kumar Gupta; Birinchi K. Sarma; Anil Rai; Tusar Kanti Behera
Vegetable crops possess a prominent nutri-metabolite pool that not only contributes to the crop performance in the fields, but also offers nutritional security for humans. In the pursuit of identifying, quantifying and functionally characterizing the cellular metabolome pool, biomolecule separation technologies, data acquisition platforms, chemical libraries, bioinformatics tools, databases and visualization techniques have come to play significant role. High-throughput metabolomics unravels structurally diverse nutrition-rich metabolites and their entangled interactions in vegetable plants. It has helped to link identified phytometabolites with unique phenotypic traits, nutri-functional characters, defense mechanisms and crop productivity. In this study, we explore mining diverse metabolites, localizing cellular metabolic pathways, classifying functional biomolecules and establishing linkages between metabolic fluxes and genomic regulations, using comprehensive metabolomics deciphers of the plant’s performance in the environment. We discuss exemplary reports covering the implications of metabolomics, addressing metabolic changes in vegetable plants during crop domestication, stage-dependent growth, fruit development, nutri-metabolic capabilities, climatic impacts, plant-microbe-pest interactions and anthropogenic activities. Efforts leading to identify biomarker metabolites, candidate proteins and the genes responsible for plant health, defense mechanisms and nutri-rich crop produce are documented. With the insights on metabolite-QTL (mQTL) driven genetic architecture, molecular breeding in vegetable crops can be revolutionized for developing better nutritional capabilities, improved tolerance against diseases/pests and enhanced climate resilience in plants. © 2022 by the authors.
Phenolic acids in some Indian cultivars of Momordica charantia and their therapeutic properties
(2011) Udai Pratap Singh; Sudarshan Maurya; Amitabh Singh; Mandavi Singh
Bitter gourd (Momordica charantia) is an important medicinal plant consumed mostly as vegetable. Often the whole plant is used in different forms for improving human health. Phenolic acid analysis by high performance liquid chromatograph (HPLC) of three cultivars of M. charantia (viz., Pusa Vishesh, Kalyanpur Barasati and Priya) has been done. Kalyanpur Barasati was rich in some phenolic acids (six phenolic acids) followed by Pusa Vishesh and Priya (five phenolic acids). Gallic, caffeic, chlorogenic and ferulic acids were detected in fruit parts of all the three varieties where gallic acid was in maximum amount. Caffeic acid was maximum in Pusa Vishesh and Kalyanpur Barasati. In roots and leaves, phenolic acids were detected in traces. The importance of fruits which are consumed as vegetable in human diet has been discussed in the light of phenolic acid content. © 2011 Academic Journals.
Rhizobium-mediated induction of phenolics and plant growth promotion in rice (Oryza sativa L.)
(2006) Ravi P. N. Mishra; Ramesh K. Singh; Hemant K. Jaiswal; Vinod Kumar; Sudarshan Maurya
Qualitative and quantitative estimation of phenolic compounds was done through reverse phase-high performance liquid chromatography (RP-HPLC) from different parts (leaf, stem, and root) of rice plants after inoculation with two rhizobial strains, RRE6 (Rhizobium leguminosarum bv. phaseoli) and ANU 843 (R. leguminosarum bv. trifolii) and infection by Rhizoctonia solani. On the basis of their retention time, the major phenolic acids detected in HPLC analysis were gallic, tannic, ferulic, and cinnamic acids. Furthermore, in all Rhizobium-inoculated rice plants, synthesis of phenolic compounds was more consistently enhanced than in control (uninoculated plants), where the maximum accumulation of phenolic compounds was observed in plants inoculated with RRE6 and infection with R. solani. Under pathogenic stress, RRE6 performed better because a relatively higher amount of phenolics was induced as compared with plants treated with ANU 843. Phenolic acids mediate induced systemic resistance and provide bioprotection to plants during pathogenic stresses. In addition, both rhizobial strains promote growth and productivity of rice plants in greenhouse conditions. This report on Rhizobium-mediated defense responses and growth promotion of nonlegume (such as rice) provides a novel paradigm of symbiotic plant-microbe interaction. © Springer Science+Business Media, Inc. 2006.
Role of air and light in sclerotial development and basidiospore formation in Sclerotium rolfsii
(2010) Sudarshan Maurya; Udai Singh; Rashmi Singh; Amitabh Singh; Harikesh Singh
Sclerotium rolfsii is one of the devastating soil-borne phytopathogens which causes severe loss at the time of seedling development. It also causes leaf spots in several crops and wild plants. Petri plates, containing potato dextrose agar medium, were inoculated with S. rolfsii. Two-third area of three, 50% area of three and 100% area of other three plates were sealed with cellophane tape. The other three plates were not sealed. All the plates were incubated at 27±2°C. Two sets of such plates were prepared. One set was incubated in light whereas the other set in the dark. There was no significant difference in mycelial growth and number of sclerotia among them but significant difference was observed when compared to the control, i.e. the plates which were not sealed. Sclerotium and basidiospore formation were directly influenced by air as completely sealed plates failed to produce sclerotia and basidiospores. Basidiospores were produced abundantly in the light and in the dark conditions in unsealed plates only on Cyperus rotundus rhizome meal agar medium.
Roots of resistance: Unraveling microbiome-driven plant immunity
(Elsevier B.V., 2024) Dhananjaya Pratap Singh; Sudarshan Maurya; Lovkush Satnami; Renu; Ratna Prabha; Birinchi K. Sarma; Nagendra Rai
The intricate interplay between microbiome and plant immunity represents a frontier in plant biology with significant implications for agriculture and ecosystem management. This review explores intricate relationship between plant immunity and the microbiome, highlighting its significance in addressing current agricultural and environmental challenges. The plant immune system, comprising pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), plays crucial role in shaping microbial communities in the rhizosphere. Phytohormones such as salicylic acid, jasmonic acid, and ethylene are the key modulators of plant defenses and contribute to rhizosphere microbiome composition. The concept of defense priming and plant immune memory emerges as a promising avenue for enhancing crop resilience against phytopathogens and environmental stresses. Root exudates and plant defense signatures actively influence rhizosphere microbiome structure, establishing a bidirectional relationship between plants and their microbial partners. This interaction is particularly relevant in the context of climate change, where plants face increasing biotic and abiotic stresses. Understanding and leveraging these complex interactions holds promise for developing more sustainable agricultural practices, reducing reliance on chemical inputs, and ensuring food security in the face of global challenges. We have stressed upon the importance of viewing the plant-soil-microbiome system as an integrated unit or holobiont. As agriculture grapples with the challenges of feeding a growing population under changing environmental conditions, harnessing the power of plant-microbiome interactions presents a promising strategy for improving food security and promoting ecosystem health. © 2024 The Author(s)
The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens
(Sociedade Brasileira de Microbiologia, 2016) Singh Rashmi; Sudarshan Maurya; Ram Sanmukh Upadhyay
The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagenesis of these two selected strains was performed. Two mutants, Th-m1 and T. atroviride m1, were found to be more effective than their parent strains. The enzymatic activities of the selected parent and mutant strains were assayed, and although both mutants were found to have enhanced enzymatic activities compared to their respective parent strains, Th-m1 possessed the maxi-mum cellulase (5.69 U/mL) and β-1,3-glucanase activity (61.9 U/mL). Th-m1 also showed high competitive saprophytic ability (CSA) among all of the selected parent and mutant strains, and during field experiments, Th-m1 was found to successfully possess enhanced disease control (82.9%). © 2015 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Therapeutic importance and analysis of phenolic acids in different parts of Syzygium cumini Linn
(IOS Press BV, 2013) Udai Pratap Singh; Sudarshan Maurya; Shraddha Nayak; Mandavi Singh; Amitabh Singh
Jamun (Syzygium cumini), which belongs to the family Myrtaceae, is an important indigenous minor fruit of commercial value. S. cumini fruit possesses considerable nutritive value because it is a rich source of carbohydrates, proteins, iron, calcium, minerals, vitamins as well as phenolic acids. Leaves are rich in phenolic acids in which tannic acid was maximum followed by caffeic acid. Tannic acid is also maximum in ripe fruit epicarp, seeds and mesocarp as compared to unripe fruit epicarp. Caffeic and cinnamic acids are also present in ripe fruit seeds. Other phenolic acids are present in traces. Tannic acid was maximum in the seeds of unripe fruits followed by chlorogenic and gallic acids and some other phenolic acids. The therapeutic properties of these phenolic acids are discussed. © 2013 Springer-Verlag Italia.
Untargeted Metabolomics of Alternaria solani-Challenged Wild Tomato Species Solanum cheesmaniae Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways
(MDPI, 2023) Dhananjaya Pratap Singh; Mansi Singh Bisen; Ratna Prabha; Sudarshan Maurya; Suresh Reddy Yerasu; Renu Shukla; Jagesh Kumar Tiwari; Krishna Kumar Chaturvedi; Md. Samir Farooqi; Sudhir Srivastava; Anil Rai; Birinchi Kumar Sarma; Nagendra Rai; Prabhakar Mohan Singh; Tusar Kanti Behera; Mohamed A. Farag
Untargeted metabolomics of moderately resistant wild tomato species Solanum cheesmaniae revealed an altered metabolite profile in plant leaves in response to Alternaria solani pathogen. Leaf metabolites were significantly differentiated in non-stressed versus stressed plants. The samples were discriminated not only by the presence/absence of specific metabolites as distinguished markers of infection, but also on the basis of their relative abundance as important concluding factors. Annotation of metabolite features using the Arabidopsis thaliana (KEGG) database revealed 3371 compounds with KEGG identifiers belonging to biosynthetic pathways including secondary metabolites, cofactors, steroids, brassinosteroids, terpernoids, and fatty acids. Annotation using the Solanum lycopersicum database in PLANTCYC PMN revealed significantly upregulated (541) and downregulated (485) features distributed in metabolite classes that appeared to play a crucial role in defense, infection prevention, signaling, plant growth, and plant homeostasis to survive under stress conditions. The orthogonal partial least squares discriminant analysis (OPLS-DA), comprising a significant fold change (≥2.0) with VIP score (≥1.0), showed 34 upregulated biomarker metabolites including 5-phosphoribosylamine, kaur-16-en-18-oic acid, pantothenate, and O-acetyl-L-homoserine, along with 41 downregulated biomarkers. Downregulated metabolite biomarkers were mapped with pathways specifically known for plant defense, suggesting their prominent role in pathogen resistance. These results hold promise for identifying key biomarker metabolites that contribute to disease resistive metabolic traits/biosynthetic routes. This approach can assist in mQTL development for the stress breeding program in tomato against pathogen interactions. © 2023 by the authors.